• Title/Summary/Keyword: Species Comparison

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PCR-based markers developed by comparison of complete chloroplast genome sequences discriminate Solanum chacoense from other Solanum species

  • Kim, Soojung;Park, Tae-Ho
    • Journal of Plant Biotechnology
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    • v.46 no.2
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    • pp.79-87
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    • 2019
  • One of wild diploid Solanum species, Solanum chacoense, is one of the excellent resources for potato breeding because it is resistant to several important pathogens, but the species is not sexually compatible with potato (S. tuberosum) causing the limitation of sexual hybridization between S. tuberosum and S. chacoense. Therefore, diverse traits regarding resistance from the species can be introgressed into potato via somatic hybridization. After cell fusion, the identification of fusion products is crucial with molecular markers. In this study, S. chacoense specific markers were developed by comparing the chloroplast genome (cpDNA) sequence of S. chacoense obtained by NGS (next-generation sequencing) technology with those of five other Solanum species. A full length of the cpDNA sequence is 155,532 bp and its structure is similar to other Solanum species. Phylogenetic analysis resulted that S. chacoense is most closely located with S. commersonii. Sequence alignment with cpDNA sequences of six other Solanum species identified two InDels and 37 SNPs specific sequences in S. chacoense. Based on these InDels and SNPs regions, four markers for distingushing S. chacoense from other Solanum species were developed. These results obtained in this research could help breeders select breeding lines and facilitate breeding using S. chacoense in potato breeding.

DNA Barcoding of Benthic Ragworms of the Genus Nectoneanthes (Polychaeta: Nereididae) Collected in Korean Waters

  • Park, Taeseo
    • Animal Systematics, Evolution and Diversity
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    • v.37 no.3
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    • pp.235-241
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    • 2021
  • To provide better taxonomic information of the genus Nectoneanthes, the two DNA barcode regions of mitochondrial cytochrome c oxidase subunit I (COI) and 16S ribosomal DNA (rDNA) sequences of Nectoneanthes oxypoda and N. uchiwa were determined. In addition, the respective sequences of four nereidid species closely related to Nectoneanthes were retrieved from GenBank for comparison and to estimate intra- and inter-specific genetic distances. The aligned sequence lengths of COI and 16S rDNA were 570 bp and 419 bp long, respectively. The mean intraspecific variation in both markers was less than 1% in all species except for that in COI of H. diadroma (1.87%). The mean interspecific variation between N. oxypoda and N. uchiwa was 12.02% regarding COI and 1.85% regarding 16S rDNA. In contrast, the mean interspecific variation between species of other genera was comparably higher(i.e., genus Perinereis: 20.5% in COI and 8.3% in 16S rDNA; genus Hediste: 13.18% in COI and 2.64% in 16S rDNA), compared with that between the two Nectoneanthes species. This result indicated that these Nectoneanthes species are genetically more closely related than other congeneric species of different genera. The DNA barcoding information on Nectoneanthes species generated in this study provides valuable insights for further biodiversity studies on nereidid species.

Comparison of ecophysiological and leaf anatomical traits of native and invasive plant species

  • Rindyastuti, Ridesti;Hapsari, Lia;Byun, Chaeho
    • Journal of Ecology and Environment
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    • v.45 no.1
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    • pp.24-39
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    • 2021
  • Background: To address the lack of evidence supporting invasion by three invasive plant species (Imperata cylindrica, Lantana camara, and Chromolaena odorata) in tropical ecosystems, we compared the ecophysiological and leaf anatomical traits of these three invasive alien species with those of species native to Sempu Island, Indonesia. Data on four plant traits were obtained from the TRY Plant Trait Database, and leaf anatomical traits were measured using transverse leaf sections. Results: Two ecophysiological traits including specific leaf area (SLA) and seed dry weight showed significant association with plant invasion in the Sempu Island Nature Reserve. Invasive species showed higher SLA and lower seed dry weight than non-invasive species. Moreover, invasive species showed superior leaf anatomical traits including sclerenchymatous tissue thickness, vascular bundle area, chlorophyll content, and bundle sheath area. Principal component analysis (PCA) showed that leaf anatomical traits strongly influenced with cumulative variances (100% in grass and 88.92% in shrubs), where I. cylindrica and C. odorata outperformed non-invasive species in these traits. Conclusions: These data suggest that the traits studied are important for plant invasiveness since ecophysiological traits influence of light capture, plant growth, and reproduction while leaf anatomical traits affect herbivory, photosynthetic assimilate transport, and photosynthetic activity.

Studies on Planting Distrfbutien Status of Landscaping Plants in Korea (우리나라 조경 수목의 식재 분포에 관한 연구)

  • 방광자;이종석
    • Journal of the Korean Institute of Landscape Architecture
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    • v.23 no.1
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    • pp.67-94
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    • 1995
  • A total of 394 plant species in 74 families planted in home gardens, public building gardens, campuses of elementaiy, middle, and high schools, and universities, recreation areas, city parks, loeal and national ways, and highways from 1.21 local areal in Korea were surveyed- A comparison of the surveyed species to nation-widei.y growing species in those areas were studied. the results obtained were as follows : 1. The ratio of evergreen to deciduous plant species was 38.1:61,9 and that of tree:scrub:vine was 54:40.9:5.1. 2. The ratio of evergreen to deciduous plant species was 27.5:72.5 among total 276 land-scape plant species surveyed in Seoul and Kyongki-do province. The ratios were 32:68 in Kwangwon-do, 36.1:63.9 ill Chungchongbuk-do, 37:61 iii Chungchongnanl-do, 36.4:63.6 in Kyungsangbuk-do, 39.2:60.8 고 Kyungsangaaal-do, 35.7:64.5 in Jollabuk-do, 40.6:59.4 in Jollanam-do, and 43.1 :56.9 in Cheju-do. This results tell that more evergreen species are growing in the southern than in northern region in Korea. 3. Plants with 100% frequency in national planting were 8 species inclubing Forsythia koreana 91-99% were 6 species including Taxus cuspidata, 81-90% were 13 species including Magnolia liilflora, 71-80% were 10 speces including Buxus microphylla val, koreana, 61-70% were 13 species including Thuja occiduntakus, 51-60% were 15 species including Pius thunbergii, 41-50% were 15 species including Kerria japonica, 31-40% were 17 species including Cryptomeria japonica, 21-30% were 28 species including Viturnum awabuki, and below 20% were 267 species including Koelreuteria paniculata. 4 Uppermost north latitude of planting distribution of major temperature species was sum- marized as follows: Although it has been reported that Cryptomeria japonica is possible to grow in areas around -47, the result from this study shows that Cryptomeria japonica grows normally in Choonchun Seoul, Samchuck, and Kwangryeung, etc. , and is possible to grow normally in the areas with approximately -6$^{\circ}C$ in an average temperature in January, where are -2$^{\circ}C$ colder than the reported temperature, but locally warm Phyllostachys bambusoides is distributed to Mt. Kumkang and Mt. Sulak, and also planted in Seoul, Kwangryeung, and Chunchon.

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The Development of Climax Index by Analysis of Eco-morphological Characters for Major Deciduous Tree Species

  • Kim, Ji Hong;Chung, Sang Hoon;Lee, Jeong Min;Kim, Se Mi
    • Journal of Forest and Environmental Science
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    • v.28 no.4
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    • pp.199-204
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    • 2012
  • This study was conducted to estimate climax index by eco-morphology for major 36 tree and sub-tree species in natural deciduous forests so as to interpret seral position of each species in the forest community. Fourteen eco-morphological characters which were considered to be associated with successional gradient in the forest were selected for the study. Four levels per character for each species were given on a standardized scale of increasing climax, and the index was computed by the proportion of the sum of total scores, expressed by percent values. With calculated mean value of 54.8 for all indices, Carpinus cordata had the highest index value of 90.5, and Populus davidiana recorded the lowest of 13.2. The most climax group, greater than 70 of the index, contained only 8 species, intermediate group, between 41 to 70 of the index, had 23 species, and the most pioneer group, less than 40 of the index comprised 5 species. The result has noticed that the large number of species would take advantage of most diverse resource and niche in the intermediate stage of the sere in the forest. By cluster analysis all 36 species were subjected to be classified into several species groups which had common similar eco-morphological characteristics. The indices were additionally plotted on the two dimensional graph to recognize the positions related to the light absorption factor and reproduction factor. The climax index of tree and sub-tree species developed by this study could be applied to understand the present status of successional stage on the basis of species composition by the method of summing up the indices. And comparison of forest successional stage among various forest communities could be done by summing up the climax indices of composed species in each community. However, this kind of applied methodology should be limited to the forest of similar species composition and site condition.

Proximate Composition of Viscera of Major, Common and Some Chinese Carps Under the Effect of Different Fertilization Schemes and Feed Supplementation in Composite Culture System

  • Mahboob, Shahid;Sheri, A.N.
    • Asian-Australasian Journal of Animal Sciences
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    • v.11 no.2
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    • pp.196-200
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    • 1998
  • The proximate composition of viscera of six fish species viz., Catla catla, Labeo, rohita Cirrhina mrigala, Hypophthalmichthys molitrix, Ctenopharyngodon idella and Cyprinus carpio under the influence of artificial feed $(T_1)$, broiler manure $(T_2)$, buffalo manure $(T_3)$, N:P:K (25:25:0 $T_4$) and control pond $(T_5)$ has been studied on final harvest after the end of experimental period of one year. The overall comparison of six fish species under five different treatments showed that Cyprinus carpio remained the best for visceral total protein contents. The maximum visceral protein content was recorded in fishes under $T_2$ except Cirrhina mrigala, while the minimum under $T_5$. The comparison of five treatments showed that maximum (6.69) percentage of fat in viscera of fish was recorded under $T_2$, closely followed by $T_4$ while minimum (4.89) under the effect of $T_5$. Among the six fish species under five treatments Cyprinus carpio showed maximum accumulation/deposition of total fats in viscera.

Comparison of the Turf Performance of Bluegrasses, Fescues, Ryegrasses, and Zoysiagrass Under a Tree Shade (수목 그늘 환경에서 블루그라스속.훼스큐속.라이그라스속 및 한국잔디의 내음성 비교연구)

  • 김경남;남상용
    • Asian Journal of Turfgrass Science
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    • v.13 no.1
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    • pp.37-54
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    • 1999
  • This study was initiated to evaluate the seasonal turf performance in a tree shade and to suggest shade-tolerant turfgrasses suitable for domestic climate conditions. Atotal of 21 trufgrasses were tested, comprising of Korean lawngrass, shade-tolerant fine fescues, and newly-developed, shade-tolerant varieties of cool-season grasses. Dirrerences in shade tolerance were observed among varieties, species, and genera. Overall turf performance of C3 turfgrasses was better than that of C4 Korean lawngrass under a tree shade. Coarse-type fescues were excellent in shade tolerance, bluegrasses good, ryegrasses medium, fine-type fescues fair, and zoysiagrasses poor, respectively. Inter-species comparison in relative index of shade tolerance(IRST) demonstrated that tall fescue and fough bluegrass were greatest of 7.3, Poa supina 6.4, perennial ryegrass 5.0, Kentucky bluegrass 4.8, and fine fescues least, respectively. A great variation in RIST was observed with fine fescues; creeping red fescue was 3.6, chewings fescue 2.5, hard fescue 2.1, and sheep fescue 1.4, respectively. Among 21 turfgrasses evaluated, tall fescue 'Rebel Jr.', 'Era', and 'Oixie' and rough bluegrass 'Sabre' were the shade-tolerant varieties under a tree shade in Korea. Tall fescue, 'Rebel Jr.' was considered as the most shade-tolerant variety in the experiment. Fine fescues as creeping red fescue, chewings fescue, hare fescue, and sheep fescue, used as the shade-tolerant species in a cool climate of Europe and North America, were not suitable under domestic climate conditions.

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Comparison of bivalves of Family Pinnidae from Southern Vietnam: A morphometric approach

  • Silina, Alla V.
    • The Korean Journal of Malacology
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    • v.27 no.4
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    • pp.345-351
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    • 2011
  • Comparison of different morphological characteristics of bivalves is very useful for distinguishing species. Therefore, this study used a morphometric approach to document patterns of phenotypic change through the specimens of family Pinnidae inhabiting the bottom sediments at the coasts of An Thoi Archipelago (south-eastern Gulf of Thailand), in that way excluding intraspecific variations among different populations along a wide geographical range. It was revealed that individuals determined as Pinna trigonium separated from specimens of Pinna nigra and Atrina vexillum at high level, but P. nigra and A. vexillum were the single species A. vexillum, as it is assumed now. Also, it was found that both groups of P. trigonium individuals and A. vexillum specimens divided into tree subgroups unified the specimens with the similar morphometric parameters: small, medium and large within the populations, though there were no evident divisions into subgroups in the size distributions of the populations. In addition to the interspecific morphological variations, the species also showed significant intraspecific morphological variations even in the same population. Most probable reason for the appearance of the intraspecific morphological variations and division of all specimens into size-specific subgroups in the population is the fact that the growth of Pinnidae bivalves is not isometric.

Genetic Diversity and Population Structure of Kaloula borealis (Anura, Microhylidae) in Korea

  • Yang, Suh-Yung;Kim, Jong-Bum;Min, Mi-Sook;Suh, Jae-Hwa;Kang, Young-Jin
    • Animal cells and systems
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    • v.4 no.1
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    • pp.39-44
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    • 2000
  • To assess the genetic diversity and population structure of Korean K. borealis, allozyme analysis was performed. The average genetic variability of Korean K. borealis populations was %P=13.2, Ho=0.048, and He=0.045. This value was the lowest in comparison with other Korean amphibian species studied. Also, the value was much lower than that of a reference population from Chinese K. borealis (%P=50, Ho=0.125, He=0.172). Wright's F-statistics showed that Korean K. borealis has distinctly low level of gene flow among regional populations (F$_{ST}$=0.339, Nm=0.487) in comparison with other Korean amphibian species studied. However, the average level of genetic divergence among Korean K. borealis populations was moderate (Nei's D=0.020). Therefore, it appeared that low levels of genetic diversity (He=0.045) and gene flow (Nm=0.487) among regional populations ave probably due to the results of decreasing population size and patchy distribution of this species in Korea.

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Fourteen new species of Allobathynella Morimoto and Miura, 1957 from South Korea: with a redescription of A. coreana Morimoto, 1970 (Crustacea, Bathynellacea, Parabathynellidae)

  • Park, Jong-Geun;Cho, Joo-Lae
    • Journal of Species Research
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    • v.5 no.1
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    • pp.49-156
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    • 2016
  • Fourteen new species and A. coreana Morimoto, 1970 of Allobathynella Morimoto and Miura, 1957 are (re-)described and illustrated from the Korean Penninsula. Comparison of the external morphology of these species and two previously known species, A. japonica Morimoto and Miura, 1957 and A. shinjongieei Park and Cho, 2008 enables us to amend the generic diagnosis. A 7-segmented antennule turns out to be inconsistent character due to A. wonjuensis sp. nov., which has a 6-segmented antennule. On the other hand, the exopodal distal segment of thoracopods I-VII with a tiny terminal knob and with outer seta covered by long and strong barbs is proved to be a autapomorphic character of the genus. The 14 new species belong to the mirabilis-group and display more or less similar morphology. However, they differ from A. coreana, A. japonica and A. shinjongieei and from each other not only in quantitative but also in qualitative characters. These differences are summarized in tables. The species inhabit hyporheic zones of rivers and each appears to be highly endemic to a given tributary, having a range limit less than 100 km.