• 한국환경독성학회:학술대회논문집
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• 한국환경독성학회 2003년도 추계국제학술대회
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• pp.158-158
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• 2003

Bioassays using gametes of sea urchins are widely used in ecotoxicological assessments of marine environments. Since most of sea urchin species in Korean coastal water spawn from spring to autumn, bioassay with them during the winter is impossible. In the course of developing standard methods for bioassays with Korean species, we found a winter-spawning starfish, Asterias amurensis, Since reproductive mode of asteroids is similar to echinoids, the bioassay protocol for sea urchins could be applied similarly to the starfish. Here, we tested and determined several conditions for the acceptability of bioassay with A. amurensis. The least required time for formation of fertilization membrane of fertilized eggs to be easily distinguished from unfertilized ones was 60 min. The threshold of sperm to egg ratio that could make acceptable fertilization rates in controls was 3000. The allowed time for manipulation of sperm after dilution in seawater was at most 3 hr. The optimal exposure time of sperms when the response against toxicant solution was relatively stable was in the range of 20-60 min. The tolerance range of sperms to the salinity of test solution was 26-38 psu. The sensitivity of A. amurensis sperm was intermediate among marine organisms commonly used in aquatic toxicity tests. The sperm bioassay with A. amurensis can be satisfactorily applied to toxicity assessments of marine environments.

• 한국양식학회지
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• 제21권1호
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• pp.34-40
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• 2008

갯지렁이류 중 산업적으로 가치가 높은 바위털갯지렁이 Marphysa sanguinea의 인공종묘생산 기술 개발을 위하여 산란습성, 유생발달 및 치충의 성장에 미치는 사육환경의 영향을 조사하였다. 바위털갯지렁이는 수온 $18^{\circ}C{\sim}22^{\circ}C$에 서식공 주변으로 담륜자(trochophore) 시기의 유생을 방출하였고, 방출된 유생은 서식공 주변에 잠시 머무른 후 수조 전체로 퍼져나가는 것이 관찰되었다. 서식공에서 방출된 유생은 8일이 지나면 개구하면서 점액질이 분비되어 몸 전체를 둘러싸는 것이 관찰되었고, 방출 후 20일이 지나면 10체절을 형성하였다. 시판용 모래의 입도별 선택성을 조사한 결과 생존율은 ${\phi}1{\sim}2mm$인 실험구에서 가장 높았고, ${\phi}2{\sim}3mm$ 실험구에서 가장 낮게 나타났다. 수온에 따른 치충의 성장은 $24^{\circ}C$ 실험구의 성장이 가장 좋았고, 50일간의 실험기간동안 이 실험구의 일간성장률은 1.1%을 나타내어 $21^{\circ}C,\;18^{\circ}C$ 및 $15^{\circ}C$구보다 2-3배가 높은 체중과 일간 성장률을 보였다. 염분 또한 치충의 성장과 생존율에 큰 영향을 주었으며, 30 psu 조건에서 가장 높은 증중 및 성장률을 보였다.

• 수산해양기술연구
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• 제28권4호
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• pp.327-336
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• 1992

In order to establish one of the forecasting model for the fishing conditions of squid angling fisheries in the Eastern Korea Sea, the catch data for the years of 1955~1991 and the water temperature data for the years of 1979~1990 were analysed, and then some parameters, that is, the water temperature normal year anomaly in the spawning and the rapidly growing season, the adult resource amount and etc were examined statistically correlation with the catch fluctuation of the main fishing seasons. From the result, authors suggested a formula as a forecasting model, Y=25785+1099X sub(1)+1074X sub(2)+6.033X sub(3)+3.95X sub(4)+1.330X sub(5)(M/T)(R super(2)=0.867, P<0.01) in the case that Y is the yearly catch, X sub(1) and X sub(2) are the water temperature normal year anomalies in October and December of the previous year and that in February and April, and X sub(3), X sub(4) and X sub(5) are the catches in October, in September, in November of previous year respectively. Because these parameters could be checked in earlier time of a half year before the main fishing season, this model was assumed to be very useful for the prediction of fishing conditions of squid angling fisheries.

• 한국수산과학회지
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• 제8권3호
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• pp.185-195
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• 1975

1. 본 논문은 1969연부터 1974연에 이르기까지 인천 송도, 안면도, 부안 근해에서 채집한 대합 M. lusoria와 가무락 C. sinensis의 산란 유발, 초기 발생 및 유생 성장에 관한 연구를 한 것이다. 2. 대합은 4/100와 5/100N의 $NH_4OH$ 해수용액을 난소에 직벚 주사하여 $25.0\~33.3\%$ 산란유발이 가능했고, 가무락에서는 1/100, 1/180, 3/100 N의 $NH_4OH$의 해수 용액으로 $12.5\~19.0\%$의 산란 유발을 할 수 있었다. 그러나 가무락의 경우는 반복온도 자극에 의해서 $40.0\%$까지 방란 및 방정 유발을 가능하게 했다. 3. 대합과 가무락에 있어서의 각각 $36.9\%$와 $39.3\%$였다. 4. 1/1000N $NH_4OH$, 해수용액에 의한 대합과 가무락의 초기 발생과정에서는 수정란 50분에 극체가 출현하고 5시간 40분이 경과되면 담륜자 유생으로 되고 2시간이 지나면 D형 피면자 유생이 됨을 관찰하였다. 5. 두 종의 유생을 사육한 결과 대합은 수정 후 1일이 지나면 평균 각장이 $10.9.5{\pm}0.7\mu$인 초기 D형기에, 11일 경과하면 $14.6{\pm}1.3\mu$ 인 각정기에, 20일이면 $208.0{\pm}0.0\mu$인 변태기에 달하였고, 가무락은 1일이 지나면 평균 각장이 $110{\mu}5{\pm}0.6\mu$인 초기 D형기에, 10일 이면 $141.8{\pm}1.7$인 각정기에, 20일이면 $235.0{\pm}0.0\mu$의 변태기에 달함을 관찰하였다. 6. 초기 D형으로부터 변태기까지의 각장(L)과 각고(H)의 상대성장 관계는 대합이 H=0.77L+6.82 이고, 가무락은 H=0.75L+8.50의 식으로 나타낼 수 있다.

• The Korean Journal of Ecology
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• 제2권1호
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• pp.9-14
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• 1978

The study was made to expand the knowledge of the ecology of Cobitis koreensis which is very common in the upper and middle part of rivers in western part of Korea. A number of C. koreensis are found in the creek of Jeonju-cheon located at Segjang-ri, Wanju-gun, Jeonrabug-do Province. The area studied has pebble bottoms. The flow velocity of the river ranges from 20cm to 40cm per second under normal conditions. The sex ratio of male to female was 1:1.34 and the female was 10~25mm larger than the male in total length. Length-frequencies in this population indicate that 20~40 mm fish group is underyearling, 40~60mm is a yearling, and 60~85mm is two-year-old. And the fish longer than 90mm in total length is regarded as being over three-year-old. Based on the ratio of gonadal weight to body weight, spawning in this species began in April and expanded well into June with water temperature 20~26。C, and the individuals became sexually mature over the two-year-old. The number of the eggs was counted from ten matured female fishes. The mean number of eggs in seven 72.6~81.4mm fishes was 908 (595-1, 229) and that of the rest three 86.0~89.0mm was 1, 674 (1, 337-2, 023). The contents of stomach in the other fifteen specimens captured in September 1977 were investigated. While the younger fed almost on the algae, the foods in the adult were largely aquatic insects.

• Fisheries and Aquatic Sciences
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• 제16권1호
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• pp.15-23
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• 2013

Normal embryonic development at a constant temperature ($18^{\circ}C$) has been described for the Siberian sturgeon Acipenser baerii (Acipenseriformes). Hormone-induced spawning and artificial insemination were performed to prepare embryonic batches for embryologic examination. After insemination, early cleavages of the Siberian sturgeon embryos continued for 7 h post-fertilization (HPF), showing the typical pattern of uneven holoblastic cleavage. Blastulation and gastrulation began at 9 HPF and 19 HPF, respectively. Epiboly formation (2/3 covered) was observed at 25 HPF during gastrulation. Neurulation was initiated with the formation of a slit-like neural groove from the blastopore at 33 HPF. During neurulation, the primary embryonic kidney (pronephros) and s-shaped heart developed. The embryos underwent progressive differentiation, which is typical of Acipenseriform species. A mass hatching was observed at 130 HPF, and the average total length of the hatched prolarvae was 10.5 mm. The hatched prolarvae possessed a typical pigment plug (yolk plug). The results of this study are valuable not only as a reference guide for the artificial propagation of Siberian sturgeon in hatcheries but also as the basis for the derivation of developmental gene expression assays for this species.

• 한국패류학회지
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• 제25권3호
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• pp.179-187
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• 2009

The effects of water temperature and salinity on embryonic development and spat survival of mussel Musculista senhousia were investigated. Embryos were incubated in water ranging from 0 to $35^{\circ}C$ and with salinity from 5‰ to 40‰. Mussel spat were tested in water from 0 to $40^{\circ}C$ and with salinity from 0‰ to 100‰. The optimal conditions for mussel embryos were $20-25^{\circ}C$ and salinity from 25‰ to 35‰, based on Within this temperature range, higher temperatures correponded to a shorter duration of the embryonic period. Optimisation of mussel spat survival was at $25-35^{\circ}C$ and salinity from 30‰ to 40‰; both values are higher than those for embryo, which hinted M. senhousia embryos are more vulnerable than spat. Temperatures below $15^{\circ}C$ were lethal for embryos, making temperature a feasible method with which to control the large population of M. senhousia in ark-shell farm during its spawning period.

• Fisheries and Aquatic Sciences
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• 제27권6호
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• pp.337-345
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• 2024

This study looked at some biometric and reproductive aspects of the Common carp (Cyprinus carpio) - the overexploited fish species in the Geray Reservoir as a foundation to develop an efficient fisheries management strategy. 50 fish samples (20 to 44 cm and 130 to 2,400 g) were collected between October and May 2022 using a cast net with a mesh size of 6 cm. The ratio of male to female C. carpio (1:0.52) was deviating from the anticipated 1:1 ratio. The absolute fecundity of C. carpio was relatively low (12,375 to 31,392 eggs). Gonadosomatic index varied from 0.01 to 4.6 (male) to 0.24-12.75 (female), and the spawning period of C. carpio extended from December to January. The length-weight relationships for males (TW = 0.0009TL^3.87) and females (TW = 0.0007L^3.97) indicate that C. carpio had positive allometric growth; which may have been caused by unsuitable habitat conditions and overfishing. There is a need to develop fisheries management like implementing integrated conservation efforts and reducing wetland farming. For this, this information provides a baseline data.

• 환경생물
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• 제27권1호
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• pp.1-5
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• 2009

1995년부터 2007년까지 군산연안의 조수웅덩이에 서식하는 돌가자미 Kareius bicololaratus (Basilewsky) 유어의 성장과 서식환경에 대해 조사하였다. 돌가자미의 부유성 치어는 3월 초순에 새만금 호 주변에서 출현하였고, 4월 중순에 군산 내초도 연안의 조수웅덩이에서 유어들이 저서생활을 시작하여 7월까지 성장하였다. 돌가자미의 유어들이 처음 조수웅덩이에 출현하는 4월 중순에 전장은 1.5$\sim$2.5cm(평균 2.1cm)이었고, 5월 중순에 2.8$\sim$3.5cm(3.3 cm), 6월 중순에 3.0$\sim$5.5cm(4.7cm), 7월 중순에는 6.0$\sim$7.2cm(6.7cm)이었다. 그러나 돌가자미의 유어는 새만금 방조제의 축조 이후 출현량이 감소하였고, 2005년 이후에는 채집이 되지 않았다. 새만금 방조제 축조 이후 염분도와 저질 변화 등의 환경변화가 새만금호 주변의 돌가자미의 산란과 성장에 영향을 미친 것으로 판단된다.

• 한국수자원학회논문집
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• 제38권7호
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• pp.545-554
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• 2005

금번 연구는 낙동강 유역의 영강, 위천, 회천, 거창위천, 청도천, 단장천에서 산란기와 성어기에 어류의 서식과 수심, 유속, 하상재료 등 수리학적 특성에 관한 자연과학과 공학의 학제간 상호 연계조사를 시행하였으며, 어종별(피라미와 갈겨니), 성장단계별(산란기와 성어기), 서식처조건별(수심, 유속과 하상재료) 어류 서식 적합도 기준을 작성하였다. 유지유량 증분법 개념의 물리적 서식처 모의시스템을 적용하여, 낙동강 유역의 주요 하천에서 어류서식에 적합한 유량을 산정하고, 그 중 가장 큰 유량값을 생태학적 추천유량으로 결정하였다. 금번 결정한 생태학적 추천유량은 내성천 $6.5\;m^3/s$ 등 $5.0\;m^3/s{\sim}10.0\;m^3/s$ 이었다. 또한 생태학적 추천유량과 기존에 산정된 생태계 필요유량 및 유황분석 결과를 비교${\cdot}$검토 하였다.