• Molecules and Cells
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• 제44권8호
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• pp.602-612
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• 2021

DNA methylation is an important epigenetic mechanism affecting genome structure, gene regulation, and the silencing of transposable elements. Cell- and tissue-specific methylation patterns are critical for differentiation and development in eukaryotes. Dynamic spatiotemporal methylation data in these cells or tissues is, therefore, of great interest. However, the construction of bisulfite sequencing libraries can be challenging if the starting material is limited or the genome size is small, such as in Arabidopsis. Here, we describe detailed methods for the purification of Arabidopsis embryos at all stages, and the construction of comprehensive bisulfite libraries from small quantities of input. We constructed bisulfite libraries by releasing embryos from intact seeds, using a different approach for each developmental stage, and manually picking single-embryo with microcapillaries. From these libraries, reliable Arabidopsis methylome data were collected allowing, on average, 11-fold coverage of the genome using as few as five globular, heart, and torpedo embryos as raw input material without the need for DNA purification step. On the other hand, purified DNA from as few as eight bending torpedo embryos or a single mature embryo is sufficient for library construction when RNase A is treated before DNA extraction. This method can be broadly applied to cells from different tissues or cells from other model organisms. Methylome construction can be achieved using a minimal amount of input material using our method; thereby, it has the potential to increase our understanding of dynamic spatiotemporal methylation patterns in model organisms.

• Journal of Electrical Engineering and Technology
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• 제11권6호
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• pp.1857-1862
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• 2016

Human activity recognition using depth information is an emerging and challenging technology in computer vision due to its considerable attention by many practical applications such as smart home/office system, personal health care and 3D video games. This paper presents a novel framework of 3D human body detection, tracking and recognition from depth video sequences using spatiotemporal features and modified HMM. To detect human silhouette, raw depth data is examined to extract human silhouette by considering spatial continuity and constraints of human motion information. While, frame differentiation is used to track human movements. Features extraction mechanism consists of spatial depth shape features and temporal joints features are used to improve classification performance. Both of these features are fused together to recognize different activities using the modified hidden Markov model (M-HMM). The proposed approach is evaluated on two challenging depth video datasets. Moreover, our system has significant abilities to handle subject's body parts rotation and body parts missing which provide major contributions in human activity recognition.

• 지능정보연구
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• 제28권1호
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• pp.329-352
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• 2022

미세먼지는 폐나 혈관에 침투해 각종 심장 질환이나 폐암 등의 호흡기 질환을 일으키는 것으로 보고되고 있다. 지하철은 일 평균 천만 명이 이용하는 교통수단으로, 깨끗하고 쾌적한 환경조성이 중요하나 지하터널을 통과하는 지하철의 운행 특성과 터널에 갇힌 미세먼지가 열차 풍으로 인해 지하역사로 이동하는 등의 문제로 지하역사의 미세먼지 오염도는 높은 것으로 나타나고 있다. 환경부와 서울시는 지하역사 공기질 개선대책을 수립하여 다양한 미세먼지 저감 노력을 기울이고 있다. 스마트 공기질 관리 시스템은 공기질 데이터 수집 및 미세먼지 농도를 예측하여 공기질을 관리하는 시스템으로 미세먼지 농도 예측 모델이 중요한 구성 요소이다. 그동안 시계열 데이터 예측에 관한 다양한 연구가 진행되어왔지만, 지하철 역사의 미세먼지 농도 예측과 관련해서는 통계나 순환신경망 기반의 딥러닝 모델 연구에 국한되어 있다. 이에 본 연구에서는 시공간 트랜스포머를 포함한 4개의 트랜스포머 기반 모델을 제안한다. 서울시 지하철 역사의 대합실을 대상으로 한 시간 후의 미세먼지 농도 예측실험을 수행한 결과, 트랜스포머 기반 모델들의 성능이 기존의 ARIMA, LSTM, Seq2Seq 모델들에 비해 우수한 성능을 나타냄을 확인하였다. 트랜스포머 기반 모델 중에서는 시공간 트랜스포머의 성능이 가장 우수하였다. 데이터 기반의 예측을 통하여 운영되는 스마트 공기질 관리 시스템은 미세먼지 예측의 정확도가 향상될수록 더욱더 효과적이고 에너지 효율적으로 운영될 수 있다. 본 연구 결과는 스마트 공기질 관리 시스템의 효율적 운영에 기여할 수 있을 것으로 기대된다.

• 한국환경과학회지
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• 제23권3호
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• pp.503-510
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• 2014

To estimate the benefit of high-resolution meteorological data for building energy estimation, a building energy analysis has been conducted over Busan metropolitan areas. The heating and cooling load has been calculated at seven observational sites by using temperature, wind and relative humidity data provided by WRF model combined with the inner building data produced by American Society of Heating Refrigeration and Air-conditioning Engineers (ASHRAE). The building energy shows differences 2-3% in winter and 10-30% in summer depending on locations. This result implicates that high spatiotemporal resolution of meteorological model data is significantly important for building energy analysis.

• 한국수자원학회:학술대회논문집
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• 한국수자원학회 2022년도 학술발표회
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• pp.148-148
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• 2022

Spatiotemporal precipitation data is one of the primary quantities in hydrological as well as climatological studies. Despite the fact that the estimation of these data has made considerable progress owing to advances in remote sensing, the discrepancy between satellite-derived precipitation product (SPP) data and observed data is still remarkable. This study aims to propose an effective deep learning model (DLM) for bias correction of SPPs. In which TRMM (The Tropical Rainfall Measuring Mission), CMORPH (CPC Morphing technique), and PERSIANN-CDR (Precipitation Estimation from Remotely Sensed Information using Artificial Neural Networks) are three SPPs with a spatial resolution of 0.25o exploited for bias correction, and APHRODITE (Asian Precipitation - Highly-Resolved Observational Data Integration Towards Evaluation) data is used as a benchmark to evaluate the effectiveness of DLM. We selected the Mekong River Basin as a case study area because it is one of the largest watersheds in the world and spans many countries. The adjusted dataset has demonstrated an impressive performance of DLM in bias correction of SPPs in terms of both spatial and temporal evaluation. The findings of this study indicate that DLM can generate reliable estimates for the gridded satellite-based precipitation bias correction.

• 대한의용생체공학회:의공학회지
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• 제28권2호
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• pp.258-264
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• 2007

Spatiotemporal changes of brain rhythmic activity at a certain frequency have been usually monitored in real time using scalp potential maps of multi-channel electroencephalography(EEG) or magnetic field maps of magnetoencephalography(MEG). In the present study, we investigate if it is possible to implement a real-time brain activity monitoring system which can monitor spatiotemporal changes of cortical rhythmic activity on a subject's cortical surface, neither on a sensor plane nor on a standard brain model, with a high temporal resolution. In the suggested system, a frequency domain inverse operator is preliminarily constructed, considering the individual subject's anatomical information, noise level, and sensor configurations. Spectral current power at each cortical vertex is then calculated for the Fourier transforms of successive sections of continuous data, when a single frequency or particular frequency band is given. An offline study which perfectly simulated the suggested system demonstrates that cortical rhythmic source changes can be monitored at the cortical level with a maximal delay time of about 200 ms, when 18 channel EEG data are analyzed under Pentium4 3.4GHz environment. Two sets of artifact-free, eye closed, resting EEG data acquired from a dementia patient and a normal male subject were used to show the feasibility of the suggested system. Factors influencing the computational delay are investigated and possible applications of the system are discussed as well.

• KSII Transactions on Internet and Information Systems (TIIS)
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• 제17권1호
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• pp.216-238
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• 2023

In intelligent transportation systems, traffic management is an important task. The accurate forecasting of traffic characteristics like flow, congestion, and density is still active research because of the non-linear nature and uncertainty of the spatiotemporal data. Inclement weather, such as rain and snow, and other special events such as holidays, accidents, and road closures have a significant impact on driving and the average speed of vehicles on the road, which lowers traffic capacity and causes congestion in a widespread manner. This work designs a model for multivariate short-term traffic congestion prediction using SLSTM_AE-BiLSTM. The proposed design consists of a Bidirectional Long Short Term Memory(BiLSTM) network to predict traffic flow value and a Convolutional Neural network (CNN) model for detecting the congestion status. This model uses spatial static temporal dynamic data. The stacked Long Short Term Memory Autoencoder (SLSTM AE) is used to encode the weather features into a reduced and more informative feature space. BiLSTM model is used to capture the features from the past and present traffic data simultaneously and also to identify the long-term dependencies. It uses the traffic data and encoded weather data to perform the traffic flow prediction. The CNN model is used to predict the recurring congestion status based on the predicted traffic flow value at a particular urban traffic network. In this work, a publicly available Caltrans PEMS dataset with traffic parameters is used. The proposed model generates the congestion prediction with an accuracy rate of 92.74% which is slightly better when compared with other deep learning models for congestion prediction.

• 한국수자원학회:학술대회논문집
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• 한국수자원학회 2021년도 학술발표회
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• pp.136-136
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• 2021

In this study, a deep convolutional neural network (DCNN) model is proposed for short-term precipitation forecasting using weather radar-based images. The DCNN model is a combination of convolutional neural networks, autoencoder neural networks, and U-net architecture. The weather radar-based image data used here are retrieved from competition for rainfall forecasting in Korea (AI Contest for Rainfall Prediction of Hydroelectric Dam Using Public Data), organized by Dacon under the sponsorship of the Korean Water Resources Association in October 2020. This data is collected from rainy events during the rainy season (April - October) from 2010 to 2017. These images have undergone a preprocessing step to convert from weather radar data to grayscale image data before they are exploited for the competition. Accordingly, each of these gray images covers a spatial dimension of 120×120 pixels and has a corresponding temporal resolution of 10 minutes. Here, each pixel corresponds to a grid of size 4km×4km. The DCNN model is designed in this study to provide 10-minute predictive images in advance. Then, precipitation information can be obtained from these forecast images through empirical conversion formulas. Model performance is assessed by comparing the Score index, which is defined based on the ratio of MAE (mean absolute error) to CSI (critical success index) values. The competition results have demonstrated the impressive performance of the DCNN model, where the Score value is 0.530 compared to the best value from the competition of 0.500, ranking 16^th out of 463 participating teams. This study's findings exhibit the potential of applying the DCNN model to short-term rainfall prediction using weather radar-based images. As a result, this model can be applied to other areas with different spatiotemporal resolutions.

• 대한원격탐사학회지
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• 제36권5_1호
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• pp.807-821
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• 2020

이 연구에서는 작물 모니터링을 위한 시계열 고해상도 영상 구축을 위해 기존 중저해상도 위성영상의 융합을 위해 개발된 대표적인 시공간 융합 모델의 적용성을 평가하였다. 특히 시공간 융합 모델의 원리를 고려하여 입력 영상 pair의 특성 차이에 따른 모델의 예측 성능을 비교하였다. 농경지에서 획득된 시계열 Sentinel-2 영상과 RapidEye 영상의 시공간 융합 실험을 통해 시공간 융합 모델의 예측 성능을 평가하였다. 시공간 융합 모델로는 Spatial and Temporal Adaptive Reflectance Fusion Model(STARFM), SParse-representation-based SpatioTemporal reflectance Fusion Model(SPSTFM)과 Flexible Spatiotemporal DAta Fusion(FSDAF) 모델을 적용하였다. 실험 결과, 세 시공간 융합 모델은 예측 오차와 공간 유사도 측면에서 서로 다른 예측 결과를 생성하였다. 그러나 모델 종류와 관계없이, 예측 시기와 영상 pair가 획득된 시기 사이의 시간 차이보다는 예측 시기의 저해상도 영상과 영상 pair의 상관성이 예측 능력 향상에 더 중요한 것으로 나타났다. 또한 작물 모니터링을 위해서는 오차 전파 문제를 완화할 수 있는 식생지수를 시공간 융합의 입력 자료로 사용해야 함을 확인하였다. 이러한 실험 결과는 작물 모니터링을 위한 시공간 융합에서 최적의 영상 pair 및 입력 자료 유형의 선택과 개선된 모델 개발의 기초정보로 활용될 수 있을 것으로 기대된다.

• 한국물환경학회지
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• 제36권6호
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• pp.508-520
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• 2020

Dam reservoirs have been reported to contribute significantly to global carbon emissions, but unlike natural lakes, there is considerable uncertainty in calculating carbon emissions due to the complex of emission pathways. In particular, the method of calculating carbon dioxide (CO2) net atmospheric flux (NAF) based on a simple gas exchange theory from sporadic data has limitations in explaining the spatiotemporal variations in the CO2 flux in stratified reservoirs. This study was aimed to analyze the spatial and temporal CO2 distribution and mass balance in Daecheong Reservoir, located in the mid-latitude monsoon climate zone, by applying a two-dimensional hydrodynamic and water quality model (CE-QUAL-W2). Simulation results showed that the Daecheong Reservoir is a heterotrophic system in which CO2 is supersaturated as a whole and releases CO2 to the atmosphere. Spatially, CO2 emissions were greater in the lacustrine zone than in the riverine and transition zones. In terms of time, CO2 emissions changed dynamically according to the temporal stratification structure of the reservoir and temporal variations of algae biomass. CO2 emissions were greater at night than during the day and were seasonally greatest in winter. The CO2 NAF calculated by the CE-QUAL-W2 model and the gas exchange theory showed a similar range, but there was a difference in the point of occurrence of the peak value. The findings provide useful information to improve the quantification of CO2 emissions from reservoirs. In order to reduce the uncertainty in the estimation of reservoir carbon emissions, more precise monitoring in time and space is required.