• Title/Summary/Keyword: Spacers

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Detection of Genus Phytophthora and Phytophthora cryptogea-P. drechsleri Complex Group Using Polymerase Chain Reaction with Specific Primers

  • Hong, Seung-Beom;Park, In-Cheol;Go, Seung-Joo;Ryu, Jin-Chang
    • The Plant Pathology Journal
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    • v.15 no.5
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    • pp.287-294
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    • 1999
  • A technique based on the polymerase chain reaction (PCR) for the specific detection of genus Phytophthora and Phytophthora cryptogea-P. drechsleri complex group was developed using nucleotide sequence information of ribosomal DNA (rDNA) regions. The internal transcribed spacers (ITS) including 5.8S were sequenced for P. cryptogea-P. drechsleri complex group and its related species. Two pairs of oligonucleotide primers were designed. Primer pair ITS1/Phy amplified ca. 240 bp fragment in 12 out of 13 specie of Phytophthora, but not in Pythium spp., Fusarium spp.and Rhizoctonia solani. Primer pair rPhy/Pcd amplified 549 bp fragment only in P. cryptogea-P. drechsleri complex group, but not in other Phytophthora spp.and other genera. Specific PCR amplification using the primers was successful in detecting Phytophthora and P. cryptogea-P. drechsleri complex group in diseased plants.

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Analysis of Intermolecular Interaction in Thermotropic Aromatic Polyurethanes with Flexible Spacers (Spacer를 가지고 방향족 polyurethane의 분자간 상호 작용에 의한 액정성의 발견)

  • Lee, Jong Back;Song, Jin Cherl
    • Textile Coloration and Finishing
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    • v.7 no.4
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    • pp.8-15
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    • 1995
  • A series of thermotropic Polyurethanes mesogenic unit were synthesized by polyaddition of a para-type diisocyanate such as 2,5-tolylene diisocyanate(2,5-TDI) with 4-4'-bis($\omega$-hydroxyalkoxy) biphenls(BPm: $HOCmH_{2m}OC_{6}H_{4}OC_{m}H_{2m}OH$ : m is the carbon number of the hydroxyalkoxy group) in DMF. Intrinsic viscosities of the polymers were in the range of 0.41~0.99dL/g DSC thermograms for these polymers exhibited two endothermic peaks corresponding to phase transitions of melting and isotropization. For examplem polyurethane 2,5-TDI/BPll with [η]=0.99 prepared from 2.5-TDI and 4,4'-bis[11-hydroxyundecaoxy biphenyl(BP11) a liquid crystalline phase from 156 to 173$^{\circ}C$. The thermotropic properites of liquid crystalline polyurethanes have been investigated by wide-angle X-ray scatter(WAXS), infrared (IR) spectroscopy, and differential scanning calorimetry (DSC). The mesomorphic behavior of the polyurethanes was concluded to be greatly dependent on the intermolecular hydrogen bonds through the urethane.

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Internal Transcribed Spacer (ITS) Regions Reveals Phylogenic Relationships of Rubus Species Cultivated in Korea (Internal Transcribed Spacers (ITS)로 분석한 국내 Rubus 재배종의 계통학적 유연관계)

  • Eu, Gee-Suck;Park, Myoung-Ryoul;Yun, Song-Joong
    • Korean Journal of Medicinal Crop Science
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    • v.17 no.3
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    • pp.165-172
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    • 2009
  • The bramble cultivated widely in South Korea, which is known as Bokbunja-ddal-gi, is regarded as having originated from Korean native Rubus coreanus. This study was carried out to obtain basic phylogenetic information on Korean cultivated bramble (KCB) by comparing the internal transcribed spacer (ITS) regions with those of R. coreanus, blackberry (R. lanciniatus), black (R. occidentalis) and red (R. idaeus) raspberry. Sequences of the ITS 1 suggest that some KCB accessions share a significant similarity with both R. occidentalis and R. coreanus in the ITS 1 region. The ITS 2 sequences of the three KCB accessions clustered more closely to those of two R. occidentalis accessions than to those of R. coreanus. These results suggest that there exist variations in the sequences of ITS among KCB accessions and KCB accessions are more closely related to black raspberry than R. coreanus in the ITS regions.

Dimesogenic Compounds with Chiral Tails: Synthesis and Liquid Crystalline Properties of a Homologous Series of a, w-Bis[4-(4'-(S)-( -)-2-methylbutoxycarbonylbiphenyl- 4-oxycarbonyl)phenoxy]alkanes

  • Choe, Lee Jun;Choe, Bong Gu;Kim, Jae Hun;Jin, Jeong Il
    • Bulletin of the Korean Chemical Society
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    • v.21 no.1
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    • pp.110-117
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    • 2000
  • A series of new liquid crystalline dimesogenic compounds with chiral tails was synthesized, and their thermal and liquid crystalline properties were studied. The chain length of the central polymethylene spacers (x) was varied from dimethylene (2) to decamethylene (12). These compounds were characterized by elemental analysis, IR and NMR spectroscopy, differential scanning calorimetry (DSC), and cross-polarizing microscopy. All compounds were found to be enantiotropically liquid crystalline, and the values of melting ($T_m$) and isotropization temperature ($T_i$) as well as enthalpy change (Δ$H_i$) and entropy change for isotropization (Δ$S_i$) decreased in a zig-zag fashion revealing the so-called odd-even effect as x increases. Their mesomorphic properties fall into three categories depending upon x; (a) compounds with x=2 and 4 formed two different mesophases, smectic and cholesteric phases in that order on heating, and vice versa on cooling, (b) compounds with x=3, 7, 8, 10 and 11 reversibly formed only the cholesteric phase, and (c) compounds with x=5, 6, 9 and 12 exhibited only a cholesteric phase on heating, whereas on cooling they formed two different mesophases, cholesteric and smectic phases, sequentially.

Isolation and Identification of Two Unreported Fungi in Korea: Dothidea insculpta and Metarhizium rileyi

  • Moe, Than Naing;Das, Kallol;Diane, Avalos-Ruiz;Kang, In-Kyu;Lee, Seung-Yeol;Jung, Hee-Young
    • The Korean Journal of Mycology
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    • v.49 no.3
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    • pp.315-324
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    • 2021
  • In this study, the fungal strains KNU-Gunwi 2B and KNU-SOT5 were isolated from root-soil in a hillside and the cherry tree bark (Prunus serrulata), respectively from Gyeongbuk province in Korea. The strain KNU-SOT5 produced dark brown chlamydospores that were smooth to lightly rough-walled, globose to ellipsoidal, and the conidia were aseptate, guttulate, mostly fusiform with a diameter of 5.3-17.6×4.2-7.0 ㎛. Strain KNU-Gunwi 2B produced phialides that were smooth-walled, cylindrical with semi-papillate apices and the conidia were pale-green, broadly ellipsoid, and sometimes cylindrical with a diameter of 4.4-8.0×2.3-4.0 ㎛. The strain KNU-SOT5 and KNU-Gunwi 2B were resolved based on cultural and morphological characteristics, along with the phylogenetic analysis using the small subunit (SSU), large subunit (LSU), and internal transcribed spacers (ITS) regions. The fungal strains KNU-SOT5 and KNU-Gunwi 2B were identified as Dothidea insculpta and Metarhizium rileyi, which have not been reported in Korea.

Effect Analysis of Spacer Stiffness and Interval on Galloping of Power Transmission Lines (스페이서 강성과 간격이 송전선 갤러핑에 미치는 영향분석)

  • Oh, Yun-Ji;Sohn, Jeong-Hyun
    • Journal of the Korean Society of Manufacturing Process Engineers
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    • v.18 no.1
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    • pp.52-58
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    • 2019
  • Due to icing and snow, power transmission lines have asymmetric cross sections, and their motion becomes unstable. At this time, the vibration caused by the wind is called galloping. If galloping is continuous, short circuits or ground faults may occur. It is possible to prevent galloping by installing spacers between transmission lines. In this study, the transmission line is modeled as a mass-spring-damper system by using RecurDyn. To analyze the dynamic behavior of the transmission line, the damping coefficient is derived from the free vibration test of the transmission line and Rayleigh damping theory. The drag and lift coefficient for modeling the wind load are calculated from the flow analysis by using ANSYS Fluent. Galloping simulations according to spacer stiffness and interval are carried out. It is found that when the stiffness is 100 N/m and the interval around the support is dense, the galloping phenomenon is reduced the most.

A report of the second chloroplast genome sequence in Veronica nakaiana (Plantaginaceae), an endemic species in Korea

  • LEE, Yae-Eun;LEE, Yoonkyung;KIM, Sangtae
    • Korean Journal of Plant Taxonomy
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    • v.51 no.1
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    • pp.109-114
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    • 2021
  • Veronica nakaiana Ohwi (Plantaginaceae) is an endemic taxon on Ulleungdo Island, Korea. We report the second complete chloroplast genome sequence of V. nakaiana. Its genome size is 152,319 bp in length, comprising a large single-copy of 83,195 bp, a small single-copy of 17,702 bp, and a pair of inverted repeat regions of 25,711 bp. The complete genome contains 115 genes, including 51 protein-coding genes, four rRNA genes, and 31 tRNA genes. When comparing the two chloroplast genomes of V. nakaiana, 11 variable sites are recognized: seven SNPs and four indels. Two substitutions in the coding regions are recognized: rpoC2 (synonymous substitution) and rpl22 (nonsynonymous substitution). In nine noncoding regions, one is in the tRNA gene (trnK-UUU), one is in the intron of atpF, and seven are in the intergenic spacers (trnH-GUG~psbA, trnK-UUU, rps16~trnQ-UUG, trnC-GCA~petN, psbZ~trnG-GCC, ycf3~trnS-GGA, ycf4~cemA, and psbB~psbT). The data provide the level of genetic variation in V. nakaiana. This result will be a useful resource to formulate conservation strategies for V. nakaiana, which is a rare endemic species in Korea.

Spatial and Temporal Genetic Diversity and Population Structure of Hemileia vastatrix from Peruvian Coffee Plantations

  • Quispe-Apaza, Cinthia;Mansilla-Samaniego, Roberto;Espejo-Joya, Rosa;Bernacchia, Giovanni;Yabar-Larios, Marisela;Lopez-Bonilla, Cesar
    • The Plant Pathology Journal
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    • v.37 no.3
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    • pp.280-290
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    • 2021
  • Population genetic studies of Hemileia vastatrix have been conducted in order to describe the evolutionary dynamics of the pathogen and the disease epidemiology as consequence of changes in disease management and host distribution occurred in Peru after the 2013 epidemic. These analyses were performed by sequencing the internal transcribed spacers of the nuclear ribosomal DNA (rDNA-ITS) of H. vastatrix collected from two coffee growing areas in 2014 and 2018. H. vastatrix population showed high haplotype diversity (Hd = 0.9373 ± 0.0115) with a low nucleotide diversity (π = 0.00322 ± 0.00018). Likewise, AMOVA indicated that fungus population has behaved as a large population without structuring by geographical origin and sampling years (FST = 0.00180, P = 0.20053 and FST = 0.00241, P = 0.19693, respectively). Additionally, the haplotype network based on intraspecific phylogenetic analysis of H. vastatrix using Peruvian and NCBI sequences revealed that Peruvian ancestral haplotypes, which were maintained in time and space, would correspond to the reported sequences of the races II and XXII. This result suggests that no substantial changes have occurred through time in Peruvian Hemileia vastatrix population.

Sensitivity Analysis of Thermal Parameters Affecting the Peak Cladding Temperature of Fuel Assembly

  • Ju-Chan Lee;Doyun Kim;Seung-Hwan Yu;Sungho Ko
    • Journal of Nuclear Fuel Cycle and Waste Technology(JNFCWT)
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    • v.21 no.3
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    • pp.359-370
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    • 2023
  • The thermal integrity of spent nuclear fuels has to be maintained during their long-term dry storage. The detailed temperature distributions of spent fuel assemblies are essential for evaluating the integrity of their dry storage systems. In this study, a subchannel analysis model was developed for a canister of a single fuel assembly using the COBRA-SFS code. The thermal parameters affecting the peak cladding temperature (PCT) of the spent fuel assembly were identified, and sensitivity analyses were performed based on these parameters. The subchannel analysis results indicated the presence of a recirculation flow, based on natural convection, between the fuel assembly and downcomer region. The sensitivity analysis of the thermal parameters indicated that the PCT was affected by the emissivity of the fuel cladding and basket, convective heat transfer coefficient, and thermal conductivity of the fluid. However, the effects of the wall friction factor of the canister, form loss coefficient of the grid spacers, and thermal conductivities of the solid materials, on the PCT were predominantly ignored.

The Study on the Uniformity, Deposition Rate of PECVD SiO2 Deposition

  • Eun Hyeong Kim;Yoon Hee Choi;Hyeon Ji Jeon;Woo Hyeok Jang;Garam Kim
    • Journal of the Semiconductor & Display Technology
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    • v.23 no.2
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    • pp.87-91
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    • 2024
  • SiO2, renowned for its excellent insulating properties, has been used in the semiconductor industry as a valuable dielectric material. High-quality SiO2 films find applications in gate spacers and interlayer insulation gap-fill oxides, among other uses. One of the prevalent methods for depositing these SiO2 films is plasma enhanced chemical vapor deposition (PECVD) favored for its relatively low processing costs and ability to operate at low temperatures. However, compared to the increasingly utilized atomic layer deposition (ALD) method, PECVD exhibits inferior film characteristics such as uniformity. This study aims to produce SiO2 films with uniformity as close as possible to those achieved by ALD through the adjustment of PECVD process parameters. we conducted a total of nine PECVD processes, varying the process time and gas flow rates, which were identified as the most influential factors on the PECVD process. Furthermore, ellipsometry analysis was employed to examine the uniformity variations of each process. The experimental results enabled us to elucidate the relationship between uniformity and deposition rate, as well as the impact of gas flow rate and deposition time on the process outcomes. Additionally, thickness measurements obtained through ellipsometer facilitate the identification of optimal process parameters for PECVD.

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