• BMB Reports
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• 제48권10호
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• pp.583-588
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• 2015

Microtubule actin crosslinking factor 1 (MACF1), a widely expressed cytoskeletal linker, plays important roles in various cells by regulating cytoskeleton dynamics. However, its role in osteoblastic cells is not well understood. Based on our previous findings that the association of MACF1 with F-actin and microtubules in osteoblast-like cells was altered under magnetic force conditions, here, by adopting a stable MACF1-knockdown MC3T3-E1 osteoblastic cell line, we found that MACF1 knockdown induced large cells with a binuclear/multinuclear structure. Further, immunofluorescence staining showed disorganization of F-actin and microtubules in MACF1-knockdown cells. Cell counting revealed significant decrease of cell proliferation and cell cycle analysis showed an S phase cell cycle arrest in MACF1-knockdown cells. Moreover and interestingly, MACF1 knockdown showed a potential effect on cellular MTT reduction activity and mitochondrial content, suggesting an impact on cellular metabolic activity. These results together indicate an important role of MACF1 in regulating osteoblastic cell morphology and function.

• 대한치과마취과학회지
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• 제12권4호
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• pp.229-233
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• 2012

Severe한 혈우병 B 환아가 소아과에 입원하여 세계혈우병연맹(World Federation of Hemophilia, WFH) 지침서에 따라서 응고인자 수준을 유지하면서 다수의 발치를 포함한 관혈적 치과 치료를 받았다. 이와 같이 혈우병 환자에서 다수의 발치를 시행할 때 주기적인 혈액검사를 통한 응고인자 수준의 관리와 복잡한 의과적 처치가 요구된다. 따라서 다수의 발치가 요구되는 경우 전신마취 하에 가능한 모든 치과치료를 시행하는 것이 효과적이고 효율적인 방법이 될 수 있다.

• Parasites, Hosts and Diseases
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• 제52권6호
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• pp.645-652
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• 2014

Fasciola hepatica is a trematode that causes zoonosis mainly in cattle and sheep and occasionally in humans. Fascioliasis has been reported in Korea; however, determining F. hepatica infection in snails has not been done recently. Thus, using PCR, we evaluated the prevalence of F. hepatica infection in snails at 4 large water-dropwort fields. Among 349 examined snails, F. hepatica-specific internal transcribed space 1 (ITS-1) and/or ITS-2 markers were detected in 12 snails and confirmed using sequence analysis. Morphologically, 213 of 349 collected snails were dextral shelled, which is the same aperture as the lymnaeid snail, the vectorial host for F. hepatica. Among the 12 F. hepatica-infected snails, 6 were known first intermediate hosts in Korea (Lymnaea viridis and L. ollula) and the remaining 6 (Lymnaea sp.) were potentially a new first intermediate host in Korea. It has been shown that the overall prevalence of the snails contaminated with F. hepatica in water-dropwort fields was 3.4%; however, the prevalence varied among the fields. This is the first study to estimate the prevalence of F. hepatica infection using the vectorial capacity of the snails in Korea.

• Bulletin of the Korean Chemical Society
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• 제29권2호
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• pp.342-346
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• 2008

Aurintricarboxylic acid (ATA) prevents apoptosis in a wide range of cell types, including PC12 cells. ATA is known to increase the phosphorylation level of IGF-1 receptor (IGF-1R) and downstream signaling proteins. ATA can translocate across the plasma membrane of PC12 cells and inhibit protein tyrosine phosphatases (PTPs) and, therefore, it is not clear whether ATA exerted its antiapoptotic effect through activation of IGF-1R or by inhibition of cytosolic PTPs. When PC12 cells, deprived of serum, were treated with Fab fragment of anti-IGF-1R antibody to prevent the binding of ATA to the extracellular domain of IGF-1R, ATA was found to penetrate into the cytosolic space of the cells. Under these conditions, the survival-promoting effects of ATA were abolished, and the increase of phosphorylation and characteristic cleavage of IGF-1R were not observed. These results indicate that the antiapoptotic effect of ATA in PC12 cells is due to the binding of ATA to the extracellular domain of IGF-1R and subsequent activation of the IGF-1R, not inhibition of cytosolic PTP(s).

• Animal cells and systems
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• 제1권2호
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• pp.329-335
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• 1997

The final pattern of the skeletal muscle of a vertebrate depends on the position-specific behavior of the muscle precursor cells during early developmental process and the abdominal muscle is made of cells which migrate a relatively long distance from their original tissue, myotome of dorsal mesoderm. We report the spatia-temporal migration pattern of abdominal muscle in Xenopus laevis by in situ hybridization and immunohistological studies. Shortly after hatching tadpole stage (stage 31/32), a group of myotomal cells detaches from the lower tip of the second somite and migrates ventrally to the lower position of abdomen. At stage 34/35, a second cell group migrates away from the third somite. Total 7 myotomal cell groups migrate ventrally one by one from the second to eighth myotome along their own pathways through the cell free space located between epidermis and subepidermal layer of the abdomen. During migration, the sizes of the cell groups (abdominal muscle anlagens) are increased to several tens fold. Around stage 40 all the abdominal muscle anlagens reaches their final positions and are interconnected side by side rostrocaudally. They are also connected to other types of muscles, forming a large multisegmented abdominal muscle. Heat shock study suggests that the disruption of segmentation of somites does not block the detachment of abdominal muscle anlagen, though the treatment gave stage- and dosagedependent effects on the migration speed.

• 한국동물학회지
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• 제23권1호
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• pp.13-24
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• 1980

소금쟁이의 精子形成에 對한 顯微鏡的 觀察을 통해 다음과 같은 結果를 얻었다. 1. Germarium은 合胞體로 된 多核의 apical complex를 포함한다. 2. Trophocyte는 不規則的인 모양을 하며 精原細胞에서 sperm bundle까지 分布해 있으며 核內 有系分裂에 의해서 크기가 증가한다. 3. 대부분의 昆蟲에 있어서는 成　이 되기 전 마지막 脫皮시에 減數分裂이 完成되는데, 本種은 成　에서도 減數分裂이 일어나서 卵子形成이 계속되며 染色體數는 2n=24로 나타났다. 4. 精子完成을 染色質濃縮, 細胞質內 顆粒, 核의 크기와 모양의 차이에 따라 7단계로 나누었으며, 이 단계들은 核의 모양에 따라 球形, 伸張形, 막대形의 3群으로 類別된다.

• Applied Microscopy
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• 제15권1호
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• pp.86-100
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• 1985

A observation of the ovarian development and oogenesis of Pieris rapae Linne has been carried out during metamorphosis using stereo-microscope, light microscope and electron microscope. The results obtained through this experiment are as follows: 1. The ovarian development and vitellogenesis begin at the 3-day old pupa and the 6-day old pupa respectively, and the adult ovary right after their emergence contains a few mature eggs. 2. The species described above are further observed at six different stages in oogenesis, and the results are summarized as follows. 1) Pieris rapae has polytrophic ovarioles. The cell organelles of the nurse cells are transfered to the oocyte through the ring canal at the early oogenesis. 2) At stage 2, the nuclear envelope of oocyte nucleus is less infolding than that of nurse cell nucleus. In the oocyte cytoplasm a large number of ribosomes are observed. 3) At stage 3 and 4, many micropinocytotic vesicles are observed in the oocyte cytoplasm. These vesicles are fused together to form large proteid yolks. 4) At stage 5, the vitelline membrane is laid down in the intercellular space between the follicle cells and oocyte. 5) At stage 6, the chorion is formed by the follicle cells. 6) A micropyle and a number of aeropyle are observed on the surface of a mature egg.

• BMB Reports
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• 제38권3호
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• pp.266-274
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• 2005

High temperature requirement A (HtrA) and its homologues constitute the HtrA familiy proteins, a group of heat shock-induced serine proteases. Bacterial HtrA proteins perform crucial functions with regard to protein quality control in the periplasmic space, functioning as both molecular chaperones and proteases. In contrast to other bacterial quality control proteins, including ClpXP, ClpAP, and HslUV, HtrA proteins contain no regulatory components or ATP binding domains. Thus, they are commonly referred to as ATP-independent chaperone proteases. Whereas the function of ATP-dependent chaperone-proteases is regulated by ATP hydrolysis, HtrA exhibits a PDZ domain and a temperature-dependent switch mechanism, which effects the change in its function from molecular chaperone to protease. This mechanism is also related to substrate recognition and the fine control of its function. Structural and biochemical analyses of the three HtrA proteins, DegP, DegQ, and DegS, have provided us with clues as to the functional regulation of HtrA proteins, as well as their roles in protein quality control at atomic scales. The objective of this brief review is to discuss some of the recent studies which have been conducted regarding the structure and function of these HtrA proteins, and to compare their roles in the context of protein quality control.

• 생명과학회지
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• 제16권7호
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• pp.1097-1103
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• 2006

Community structure varies not only in space but also in time. We stand in one position which located at Sinhung-ri, Sacheon-ci and observe the flora of area as time passes. This area begins after disturbance on sites where organisms are already present like secondary succession. In 2001, there are invaded by Trapa japonica, Salvinia natans, and Potamogon cristatus. Four or five years later, this abandoned areas support of clover, pine, and oak, resulting in a shifting pattern of species dominance and diversity through time. Patterns of community structure such as species composition and ecological diversity have shown through seasons and five years, that is the topic of this study. The values of turnover (TO) were changes through time intervals at three regions. The gradual and seemingly directional change in the structure of the community through time from aquatic like to field. Thus, we can observe the process of secondary succession at Sinhung-ri in the very short time.

• The Korean Journal of Ecology
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• 제8권2호
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• pp.61-68
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• 1985

The modeling of interception and absorption of light was studied in plant canopies at Mt. Kwanak. Followering results were obtained. Light intensity passing through the stacked leaves is attenuated exponentially. This phenomenon seems to be more clearly applied to the plant canopies, if they have large cumulative leaf area and are matured densely. Light interception and absorption are influenced by leaf thickness, shape pigments, and leaf area, and they have great effect on the maturation of canopies. It was confirmed that the light penetrating through the stratified canopies is decreased exponentially in dual pattern. The cumulative leaf area of a definite space in a certain plant canopy is the same as the growth of leaf area of the canopy at that time. A hypothetical model for calculating the light absorption in plant canopies, was established on the bases of phenomena that incident light is captured at the maximum level and light inerception effect is minimized by leaves.