Parasites, Hosts and Diseases

The Korea Society for Parasitology and Tropical Medicine (대한기생충학·열대의학회)
권호 표지
DOI QR코드

DOI QR Code

Fasciola hepatica in Snails Collected from Water-Dropwort Fields using PCR

  • Kim, Hwang-Yong (Rural Development Administration) ;
  • Choi, In-Wook (Department of Infection Biology, Chungnam National University School of Medicine) ;
  • Kim, Yeon-Rok (Rural Development Administration) ;
  • Quan, Juan-Hua (Department of Gastroenterology, The Affiliated Hospital of Guangdong Medical College) ;
  • Ismail, Hassan Ahmed Hassan Ahmed (Department of Infection Biology, Chungnam National University School of Medicine) ;
  • Cha, Guang-Ho (Department of Infection Biology, Chungnam National University School of Medicine) ;
  • Hong, Sung-Jong (Department of Medical Environmental Biology, College of Medicine, Chung-Ang University) ;
  • Lee, Young-Ha (Department of Infection Biology, Chungnam National University School of Medicine)
  • Received : 2014.09.09
  • Accepted : 2014.11.21
  • Published : 2014.12.31
Download PDF
⟨ Previous Next ⟩

Abstract

Fasciola hepatica is a trematode that causes zoonosis mainly in cattle and sheep and occasionally in humans. Fascioliasis has been reported in Korea; however, determining F. hepatica infection in snails has not been done recently. Thus, using PCR, we evaluated the prevalence of F. hepatica infection in snails at 4 large water-dropwort fields. Among 349 examined snails, F. hepatica-specific internal transcribed space 1 (ITS-1) and/or ITS-2 markers were detected in 12 snails and confirmed using sequence analysis. Morphologically, 213 of 349 collected snails were dextral shelled, which is the same aperture as the lymnaeid snail, the vectorial host for F. hepatica. Among the 12 F. hepatica-infected snails, 6 were known first intermediate hosts in Korea (Lymnaea viridis and L. ollula) and the remaining 6 (Lymnaea sp.) were potentially a new first intermediate host in Korea. It has been shown that the overall prevalence of the snails contaminated with F. hepatica in water-dropwort fields was 3.4%; however, the prevalence varied among the fields. This is the first study to estimate the prevalence of F. hepatica infection using the vectorial capacity of the snails in Korea.

Keywords

References

  1. Mas-Coma S, Bargues MD, Valero MA. Fascioliasis and other plant-borne trematode zoonoses. Int J Parasitol 2005; 35: 1255-1278 https://doi.org/10.1016/j.ijpara.2005.07.010
  2. Sripa B. Global burden of food-borne trematodiasis. Lancet Infect Dis 2012; 12: 171-172. https://doi.org/10.1016/S1473-3099(11)70321-8
  3. Novobilsky A, Engstrom A, Sollenberg S, Gustafsson K, Morrison DA, Hoglund J. Transmission patterns of Fasciola hepatica to ruminants in Sweden. Vet Parasitol 2014; 203: 276-286. https://doi.org/10.1016/j.vetpar.2014.04.015
  4. Kaplan RM, Dame JB, Reddy GR, Courtney CH. The prevalence of Fasciola hepatica in its snail intermediate host determined by DNA probe assay. Int J Parasitol 1997; 27: 1585-1593. https://doi.org/10.1016/S0020-7519(97)00139-2
  5. Schweizer G, Meli ML, Torgerson PR, Lutz H, Deplazes P, Braun U. Prevalence of Fasciola hepatica in the intermediate host Lymnaea truncatula detected by real time TaqMan PCR in populations from 70 Swiss farms with cattle husbandry. Vet Parasitol 2007; 150: 164-169. https://doi.org/10.1016/j.vetpar.2007.08.006
  6. Kozak M, Wedrychowicz H. The performance of a PCR assay for field studies on the prevalence of Fasciola hepatica infection in Galba truncatula intermediate host snails. Vet Parasitol 2010; 168: 25-30. https://doi.org/10.1016/j.vetpar.2009.10.014
  7. Son BW, Bai GH, Jo JH, Park YS, Kim SJ. A survey on bovine fascioliasis in Gyeoggi-do: prevalence survey and comparative survey and comparative chemotherapeatical experiments with niclofolan and bithionol against Fasciola spp. and Paramphistomum spp. in dairy cattle. J Korean Vet Med Ass 1977; 13: 161-164.
  8. Jang DH, Youn HJ, Jun GS. Study on metacercarial productivity of Fasciola spp. in its intermediate host Austropeplea ollula (Lymnaea ollula). Korean J Vet Res 1987; 27: 291-299.
  9. Sohn BW, Kang GS, Han TH. Studies on the optimal time for therapy of Fasciola spp. infected cattle in central area of Korea. Korean J Vet Serv 1992; 15: 1-6.
  10. Kang BK, Jung BK, Lee YS, Hwang IK, Lim H, Cho J, Hwang JH, Chai JY. A case of Fasciola hepatica infection mimicking cholangiocarcinoma and ITS-1 sequencing of the worm. Korean J Parasitol 2014; 52: 193-196. https://doi.org/10.3347/kjp.2014.52.2.193
  11. Kim YH, Kang KJ, Kwon JH. Four cases of hepatic fascioliasis mimicking cholangiocarcinoma. Korean J Hepatol 2005; 11: 169-175.
  12. Malek EA, Cheng TC. Medical and Economic Malacology. New York, USA. Academic Press. 1974.
  13. Cho SH, Lee CG, Kim JH. Laboratory maintenance of field-collected Lymnaea viridis intermediate host for Fasciola hepatica. Korean J Vet Res 1997; 37: 185-188.
  14. Jung PL. Freshwater Mollusks in Korea. 1st ed. Seoul, Korea. Yeonhag-Sa. 2003.
  15. Choe SE, Nguyen TT, Kang TG, Kweon CH, Kang SW. Genetic analysis of Fasciola isolates from cattle in Korea based on second internal transcribed spacer (ITS-2) sequence of nuclear ribosomal DNA. Parasitol Res 2011; 109: 833-839. https://doi.org/10.1007/s00436-011-2323-6
  16. Morgan JA1, Blair D. Nuclear rDNA ITS sequence variation in the trematode genus Echinostoma: an aid to establishing relationships within the 37-collar-spine group. Parasitology 1995; 111: 609-615. https://doi.org/10.1017/S003118200007709X
  17. Dung BT, Doanh PN, The DT, Loan HT, Losson B, Caron Y. Morphological and molecular characterization of lymnaeid snails and their potential role in transmission of Fasciola spp. in Vietnam. Korean J Parasitol 2013; 51: 657-662. https://doi.org/10.3347/kjp.2013.51.6.657
  18. Caron Y, Martens K, Lempereur L, Saegerman C, Losson B. New insight in lymnaeid snails (Mollusca, Gastropoda) as intermediate hosts of Fasciola hepatica (Trematoda, Digenea) in Belgium and Luxembourg. Parasit Vectors 2014; 7: 66. https://doi.org/10.1186/1756-3305-7-66
  19. Rondelaud D, Vignoles P, Abrous M, Dreyfuss G. The definitive and intermediate hosts of Fasciola hepatica in the natural watercress beds in central France. Parasitol Res 2001; 87: 475-478. https://doi.org/10.1007/s004360100385

Cited by

  1. Monitoring of Fasciola Species Contamination in Water Dropwort by COX1 Mitochondrial and ITS-2 rDNA Sequencing Analysis vol.53, pp.5, 2014, https://doi.org/10.3347/kjp.2015.53.5.641
  2. Ectopic Human Fasciola hepatica Infection by an Adult Worm in the Mesocolon vol.53, pp.6, 2014, https://doi.org/10.3347/kjp.2015.53.6.725
  3. Fasciola hepatica: Infection Status of Freshwater Snails Collected from Gangwon-do (Province), Korea vol.55, pp.1, 2017, https://doi.org/10.3347/kjp.2017.55.1.95
  4. Morphological Characterization of Emerging Cercariae among Lymnaeid Snails from Barangay Cawongan, Padre Garcia, Batangas, Philippines vol.2018, pp.None, 2018, https://doi.org/10.1155/2018/5241217
  5. A Case of Fascioliasis in A Wild Nutria, Myocastor coypus, in Republic of Korea vol.56, pp.4, 2014, https://doi.org/10.3347/kjp.2018.56.4.375
  6. Snail-borne parasitic diseases: an update on global epidemiological distribution, transmission interruption and control methods vol.7, pp.1, 2014, https://doi.org/10.1186/s40249-018-0414-7