• Applied Microscopy
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• 제44권1호
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• pp.1-7
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• 2014

The spermatogenesis of Siamese fighting fish, Betta splendens, belongs to Osphronemidae was investigated by light and electron microscopic observations. In primary spermatocyte stage, the nucleus was comparatively large ellipsoidal, and mitochondria showed a marked development in cytoplasm. In secondary spermatocyte stage, the germ cells were smaller than that of primary spermatocytes. The nucleus was a spherical shape and intercellular space was formed between germ cells. In spermatid stage, the early spermatids were not much different from a secondary spermatocyte. But, the chromatin condensation was occurred from the outside to the inside. The nucleus was more condensed. Intracellular space was larger than early spermatid. The mitochondria were rearranged in a middle piece, and occupied about half of the head part in early sperm. In sperm stage, the head of mature sperm was a spherical shape and had no acrosome. The flagellum was showed the typical 9+2 array of microtubules. Also, the tail of sperm had no lateral fins and outer coarse fibers. These ultrastructural characteristics can be used in classification of species.

• Bulletin of the Korean Chemical Society
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• 제30권11호
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• pp.2749-2753
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• 2009

Structural characteristics that influence on the insecticidal activity ($pI_{50}$) of 2-(n-octyl)isothiourea analogues (1-45) against the diamondback moth (Plutella xylostella, L.) based on three dimensional quantitative structure activity relationships (3D-QSARs) were discussed quantitatively using a comparative molecular field analysis (CoMFA) and a comparative molecular similarity indeces analysis (CoMSIA) methods. The statistical values of the CoMFA 2 model were better than those of the CoMSIA 1 model. The CoMFA 2 model was the optimized model with the correlativity (the training set: Ave. = 0.104 & PRESS = 0.613) and the predictability (the test set: Ave. = 0.086 & PRESS = 0.096). Insecticidal activities with the optimized CoMFA 2 model were dependent upon steric factors (79.4%) of $R_1-R_3$ substituents. From the analytical results of CoMFA contour maps, it is predicted that the R1 substituent of 1-45 which has a steric favor in a broad space, $R_2\;and\;R_3$ groups with a steric favor in a narrow space and a H-bond donor favor would have better the insecticidal activity.

• Applied Microscopy
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• 제24권1호
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• pp.114-122
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• 1994

Drastic changes were observed in egg chamber during oogenesis of Aedes togoi immediately after blood meal. The egg chamber, in which the oocyte remains at previtellogenic stage before blood meal, shows very little change in size even for 3 days after emergence but increased 25 folds in volume within 60 hours after blood meal, presumably due to rapid yolk formation. Upto 6 hours after blood meal structures considered to be the precursor of the yolk granules were not observed in the space between follicle cells and oocyte. Vitelline bodies, which are similar to dense bodies secreted from follicle cells, appeared in the space at 10 hours after blood meal. Although vitelline bodies were fused to form vitelline layer, these bodies seem to contribute to the formation of yolk granules. Nurse cells are connected to oocytes by cytoplasmic bridge before blood meal, but the cells are absorbed into oocyte at 6 hours after blood meal.

• BMB Reports
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• 제37권1호
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• pp.28-34
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• 2004

The discovery of biochemical and cellular functions of unannotated gene products begins with a database search of proteins with structure/sequence homologues based on known genes. Very recently, a number of frontier groups in structural biology proposed a new paradigm to predict biological functions of an unknown protein on the basis of its three-dimensional structure on a genomic scale. Structural proteomics (genomics), a research area for structure-based functional discovery, aims to complete the protein-folding universe of all gene products in a cell. It would lead us to a complete understanding of a living organism from protein structure. Two major complementary experimental techniques, X-ray crystallography and NMR spectroscopy, combined with recently developed high throughput methods have played a central role in structural proteomics research; however, an integration of these methodologies together with comparative modeling and electron microscopy would speed up the goal for completing a full dictionary of protein folding space in the near future.

• 환경생물
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• 제21권2호
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• pp.120-125
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• 2003

본 연구는 토지피복현황과 수림의 계층구조 등이 다양한 도시녹지를 대상으로 기온 및 급도에 미치는 영향물 점성 빛 정량적으로 파악하기 위해 녹지내의 기온 및 습도를 관측하였다. 그 데이터클 바탕으로 녹지내의 토지피복현황과 기온 및 습도분포, 녹지내의 수목그루와 녹적량및 습도와의 관련성을 회귀분석 둥에 의해 해석 하였다. 그 결과, 고온역은 인공구조물과 나지 주변에서, 저온역은 수림지와 수면 주변에서 형성되었다. 습도는 기온분포에 거의 대응하는 형태로 고온역이 저습역이고, 저온역이 고습역으로 나타났다. 또한, 수림의 계층구조가 2,3층으로 덮여 있는 구역과 수면을 핵으로 그 주변은 수림으로 둘러싸인 구역이 상대적으로 높은 습도를 나타냈다. 수루그루 빛 녹적량의 증가는 어떤 수고라도 습도의 상승에 효과적이고 그 효율은 교목, 소교목, 관루의 순이다.

• 대한심미치과학회지
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• 제31권1호
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• pp.4-10
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• 2022

하악 전치부에 임플란트를 이용한 수복시 몇가지 어려운 점이 있는데 그중 하나가 전 후방으로 공간이 부족하다는 것이다. 공간이 부족한 경우에 임플란트를 적절한 위치에 식립하는 것도 쉽지 않을뿐아니라 선택할 수 있는 임플란트도 제한될 수있다는 것이다. 적절한 위치에 적절한 임플란트를 식립한 경우에도 보철의 형태에따라 주변치아와 임플란트 주변골에 상당히 큰 영향을 미칠수 있는데 어떤 경우는 임플란트가 주변골을 손상시키는 위해로운 영향을 줄수도 있다. 이번 증례 발표에서는 비교적 양호한 증례와 불량한 증례를 통해서 치은하 형태(Subgingival cotour)의 차이가 임플란트 주위 Marginal bone과 인접치아의 Interproximal bone의 Biology에 다른 결과를 줄수도 있다는 것을 확인할 수있다.

• 한국동물학회:학술대회논문집
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• 한국동물학회 2001년도 한국생물과학협회 학술발표대회
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• pp.180.1-180
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• 2001

• Applied Microscopy
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• 제24권4호
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• pp.78-85
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• 1994

Sodium arsenite ($NaAsO_2$) was injected to the rat subcutaneously for the study of the acute toxicity of arsenite on hepatocytes, and the effects of pretreatment of arsenite and glutathione on the lethalty of the arsenite treated rats. Arsenite treated rat hepatocytes showed vacuolated cytosol and shrinked nuclear and expanded perinuclear space and cytoplasmic membrane whirl. Rats pretreated with BSO (L-Buthionine-SR-Sulfoximine), less survived than arsenite treated alone. It means that glutathione acts as a protecting agent against the arsenite. Subcutaneous sublethal dose (10mg/kg body weight) treatment was showed the protecting activity to lethality of lethal dose (15mg/kg body weight) treated rat. 10mg/kg body weight sublethal dose effects appeared in six hours intervals of between treatments.

• Genomics & Informatics
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• 제11권2호
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• pp.97-97
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• 2013

• Applied Microscopy
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• 제19권2호
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• pp.27-42
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• 1989

The forming process of scale and socket of Pieris rapae L. during in 30 hr. pupa to in adult was morphologically investigated with scanning electron microscopy and transmission electron microscopy. 1. The scale forming cells which were distinguished from other epidermal cells were first observed in 30 hr. pupa. In the aspect that scale forming cell beared some morphological relations to socket forming cells and in the distribution of its organelles, scale forming cell was divided into three regions-basal region in which nucleus located, neck region which was surrounded by socket forming cells and scale region that was the cytoplasmic projection region over the wing surface. In process of the development of scale forming cell neck region and scale region were extended into the molting space and at this time, the changes of surface structure of scale region have occurred initially. 2. There was a more distinct process that scale region changed into the scale. Scale region which was first originated as clublike projection of the cell body was subsequently elongated and flattened out by broadening of the cytoplasm. After that, in the surface of scale were formed longitudinal ridges and microribs. In the late pupa, the cytoplsam of scale region have autolyzed by lysosome-like bodies and at length, scale which had air spaces, trabecula, pigment granules, longitudinal ridges and transverse ridges. 3. The major protion of socket forming cell located beside neck region of scale forming cell under the wing surface but the processing portion of the cell lay over the wing surface, suggesting that socket forming cells have actively processing. In extending to the molting space of neck and scale region, socket forming cells developed to the molting space and constructed socket.