• Microbiology and Biotechnology Letters
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• v.16 no.4
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• pp.275-281
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• 1988

To develop cellulase and xylanase-producing strain by protoplast fusion, alkalophilic Bacillus sp. F204 and K17 were treated with NTG(N-methyl-N'-nitro-N-nitrosoguanidine) and isolated anti-biotics resistant strains of S20 (Km$^r$ , Cm$^r$) and G70 (Str$^r$). The frequency of protoplast formation was about 95% when cells of mid-log phase were treated with 200$\mu\textrm{g}$/ml Iysozyme at 37$^{\circ}C$ for 30-45 minutes. Under addition of 0.4-0.5M sodium succinate, 0.5% casamino acid, 1.5% polyvinylpyrrolidone, 25mM MgC1$_2$ and 50mM CaC1$_2$ to the regeneration medium, the regeneration frequency of Bacillus sp. F204 and K17 was 24.9% and 26.2%, respectively. The fusion frequency was 6.6$\times$10$^{-6}$ in the presence of 30% polyethylene glycol 6000 containing 50mM $Ca^{++}$ at 45$^{\circ}C$ for 5 minutes. Cellulase complex and xylanase activities of fusant were compared with parental strains.

• Journal of Fisheries and Marine Sciences Education
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• v.28 no.6
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• pp.1828-1833
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• 2016

The purpose of this research was to investigate the bacterial flora of healthy olive flounder larvae (Paralichthys olivaceus) and live feeds (Rotifer spp. and Artemia spp.). The total bacteria counts were $9.2{\times}10^7$ and $1.2{\times}10^{10}cfu/g$ and Vibrio sp.(82.8%) was dominant in rotifers. The total bacteria counts were $3.8{\times}10^6$ and $9.2{\times}10^6cfu/g$ and Vibrio sp.(73.3%) was dominant in artemia. In olive flounder larvae, the total bacteria counts were $1.4{\times}10^6{\sim}8.3{\times}10^7cfu/g$ and V. harveyi (38.5%) was dominant. It might be potential marker of disease outbreak in olive flounder larvae.

• Tuberculosis and Respiratory Diseases
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• v.60 no.6
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• pp.638-644
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• 2006

Backgrounds: This study investigated whether or not a polymorphism in the gene encoding the surfactant protein A(SP-A) has any bearing on the individual susceptibility to the development of chronic obstructive pulmonary disease(COPD) in a genetically homogenous Korean population. Methods: The genotypes of 19 COPD patients and 20 healthy neonates as controls were tested using a polymerase chain reaction followed by restriction fragment length polymorphism analysis for the SP-A gene. Results: The specific frequencies of the 6A2 and 6A18 alleles of SP-A1 and the 1A2 allele of SP-A2 were much higher in the COPD group than control group (p<0.05). However, the frequencies of the 6A3 and 6A4 alleles of SP-A1 and the 1A0 allele of SP-A2 in the COPD group were significantly lower than the control group. In the COPD group, the frequencies of the +50 locus genotypes GG of SP-A1 and the +9 locus genotypes CC of SP-A2 were 85.0% and 60.6%, respectively, and 19.7% and 24.8% in the control group, respectively. The frequencies of the polymorphic genotypes or alleles showed a statistically significant difference between the COPD group and the control group (P<0.05). Conclusion: A genetic polymorphism in SP-A is associated with the development of COPD in the Korean population.

• The Journal of the Korean Society for Microbiology
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• v.5 no.1
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• pp.19-26
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• 1970

1. Of the 35 various grains, 446 colonies of fungi were isolated. Among the 446 colonies, 423 were possible to identify into 17 genera. 2. Of the 10 bean-pastes, fungi were isolated 193 colonies. It can be identified 11 genera. 3. It will be noted that Aspergillus sp., Penicillium sp., Mucor sp., Cladosporium sp. were predominated genera. 4. A total of 155 colonies of Aspergillus sp. isolated from various grains were obtained from the samples. Asp. versicolor was the frequent species and 47 colonies isolated. 5. Penicillium where was isolated from various grains, obtained 115 colonies. Pen. camemberti was the most frequent species. 6. Penicillium palitans was most excellent amylase producing fungi and which was 1ml per 6.300 unit.

• Journal of Life Science
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• v.12 no.6
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• pp.765-768
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• 2002

Enantioselective hydrolysis of racemic styrene oxide by Rhodotorula sp. CL-82 was investigated. Reaction conditions including pH, temperature, and volume ratio of organic cosolvent were optimized using response surface methodology, and the optimal conditions of pH, temperature, and the volume ratio of cosolvent were determined to be 7.64, $33.26^{\circ}C$, and 3.09 %(v/v), respectively. Chiral (S)-phenyl oxirane could be obtained with high enantiomeric purity (ee ＞ 99%) and 20% yield (theoretical yield ＝ 50%) at the optimal rendition.

• Journal of Korean Neurosurgical Society
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• v.54 no.6
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• pp.525-527
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• 2013

Upward migration of the peritoneal catheter of a subgaleo-peritoneal (SP) shunt and coiling into the subgaleal space is an extremely rare complication of a SP shunt. A 32-year-old male patient visited our hospital presenting with a large skull defect due to a prior craniectomy performed elsewhere. The patient underwent a cranioplasty with methylmetacrylate, but subsequently developed progressive pseudomeningocele and subgaleal cerebrospinal fluid (CSF) collection. The patient underwent CSF diversion via a SP shunt. After SP shunting, the pseudomeningocele disappeared completely. Six months later, the patient presented with progressive scalp swelling. Skull X-ray showed migration and coiling of the distal catheter of the SP shunt. The patient was treated by removing the entire shunt catheter and the dura was covered with a subgaleal flap. We would like to report our experience with a very rare complication of subgaleo-peritoneal shunting.

• ALGAE
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• v.20 no.4
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• pp.279-293
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• 2005

Two new species of Martensia (Delesseriaceae, Rhodophyta) are described from Jeju Island, Korea. Martensia palmata sp. nov. is characterized by the following traits: more or less linearly elongated lobes arising from the distal margin of the flabellate membranous section; intact latticework with coarse and loose mesh; numerous spine-like projections on the leading margin of the latticework; tetrasporangia produced in sori along the entire blade; spermatangia borne in sori on the membranous section and the longitudinal lamellae of the latticework; and cystocarps borne on the margins of the membranous section. Martensia projecta sp. nov. has the following characteristics: subdichotomously furcated blades, with terminal segments slightly attenuated toward the apex; blades with uneven surfaces; basal parts with a multi-layered parenchymatous construction; small and poorlydefined latticework; tetrasporangia and spermatangia produced in sori on the membranous section; and cystocarps borne on the marginal surfaces of the blades. The existence of M. projecta sp. nov. supports the proposal that the genus Opephyllum Schmitz in Schmitz et Hauptfleisch should be assigned to the genus Martensia.

• Microbiology and Biotechnology Letters
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• v.23 no.5
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• pp.531-535
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• 1995

The identification of eicosapentaenoic acid (EPA) by Pseudomonas sp. CH-414 were investigated under the optimal culture conditions. The maximum dry cell weight and phospholipid production showed about 10 mg/ml and 0.6 mg/ml, respectively, at the 48 hr cultivation. The phospholipid form produced by Pseudomonas sp. CH-414 was elucidated as a phosphatidyt ethanolamine by thin layer chromatography. EPA was contained about 15% in the. extractable lipid, and the amount of EPA was about 83 $\mu $g/ml from the culture broth. Also myristic, palmitic, palmitoleic, stearic and oleic acids were identified with gas chromatography.

• The Korean Journal of Mycology
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• v.33 no.2
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• pp.92-94
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• 2005

A orchid rotting fungus was isolated and identified. The isolate was consistent with the genus Fusarium in morphological and cultural characteristics. The partial 18S rRNA sequence of the isolate showed high similarity with anamorph or telemorph of Fusarium and other Fusarium species. In phylogenetic analysis, the isolates was poorly related to other Fusarium species. The isolate closely related to Fusarium sp. LP-A2/3.

• Bulletin of the Korean Chemical Society
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• v.34 no.3
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• pp.743-748
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• 2013

The temperature dependence of the partition coefficients of a neuropeptide, substance P (SP), in isotropic acidic bicelles was investigated by using a pulsed field gradient nuclear magnetic resonance diffusion technique. The addition of negatively charged dimyristoylphosphatidylserine to the neutral bicelle changed the SP partitioning a little, which implies that the hydrophobic interaction between the hydrophobic residues of SP and the acyl chains of lipid molecules is the major interaction while the electrostatic interaction is minor in SP binding in a lipid membrane. From the temperature dependence of the partition coefficients, thermodynamic functions were calculated. The partitioning of SP into the acidic bicelles is enthalpy-driven, as it is for small unilamellar vesicles and dodecylphosphocholine micelles, while peptide partitioning into a large unilamellar vesicle is entropy-driven. This may mean that the size of lipid membranes is a more important factor for peptide binding than the surface curvature and surface charge density.