• Korean Journal of Microbiology
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• v.50 no.1
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• pp.53-59
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• 2014

This study was conducted to investigate the characteristics between non-fermented chinese yam and rice (non-FCYR) and fermented chinese yam and rice (FCYR). 1.1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity, total polyphenol, flavonoid contents and reducing power were investigated to evaluate the anti-oxidation activities of FCYR. Also, productivity of monacolin K, major medicinal ingredient of FCYR, was investigated, using Aspergillus terreus KCCM 12225. In case of non-FCYR, DPPH radical scavenging activities for three kinds of yam (Dioscorea japonica, Dioscorea batatas, Dioscorea opposita) and rice were estimated about 51.8, 66.4, 42.2 and 7.5%, while in case of FCYR, those for three kinds of yam and rice were estimated to increased about 64.7, 74.7, 52.8, and 32.3%, respectively. Total polyphenol contents of non-FCYR were estimated to 196.9, 265.7, 160.1, and 91.6 mg/kg, while total polyphenol contents of FCYR were estimated to increase about 530.7, 708.3, 427.2, and 265.9 mg/kg, respectively. Total flavonoid contents of FCYR increased to 2.8-3.3 times higher than those of non-FCYR. Reducing power of non-FCYR was about 0.97, 1.28, 0.64, and 0.17 (OD at 700 nm), while, that of FCYR increased about 1.75, 2.38, 1.24, and 0.46. Remakable increase in monacolin K productivity of FCYR was observed. Monacolin K productivity of FCYR was estimated to 467.1, 514.8, 339.2, and 272.5 mg/kg, respectively. In this study, fermented chinese yam (Dioscorea batatas) was estimated to be effective biological activity material.

• Journal of Microbiology and Biotechnology
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• v.30 no.5
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• pp.689-699
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• 2020

Brevibacillus brevis GZDF3 is a gram-positive, plant growth-promoting rhizosphere bacterium (PGPR) isolated from the rhizosphere soil of Pinellia ternata (an important herb in traditional Chinese medicine). The GZDF3 strain produces certain active compounds, such as siderophores, which are the final metabolite products of non-ribosomal peptide synthetase (NRPS) and independent non-ribosomal peptide synthetase (NIS) activity. With the present study, we attempted to investigate the siderophore production characteristics and conditions of Bacillus sp. GZDF3. The antibacterial activity of the siderophores on pathogenic fungi was also investigated. Optimal conditions for the synthesis of siderophores were determined by single factor method, using sucrose 15 g/l, asparagine 2 g/l, 32℃, and 48 h. The optimized sucrose asparagine medium significantly increased the production of siderophores, from 27.09% to 54.99%. Moreover, the effects of different kinds of metal ions on siderophore production were explored here. We found that Fe³⁺ and Cu²⁺ significantly inhibited the synthesis of siderophores. The preliminary separation and purification of siderophores by immobilized-metal affinity chromatography (IMAC) provides strong antibacterial activity against Candida albicans. The synergistic effect of siderophores and amphotericin B was also demonstrated. Our results have shown that the GZDF3 strain could produce a large amount of siderophores with strong antagonistic activity, which is helpful in the development of new biological control agents.

• Journal of Korean Clinical Nursing Research
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• v.18 no.2
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• pp.284-295
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• 2012

Purpose: This study was done to clarify effects of bispectral index monitoring sedative administration, compared to MOAA/S (Modified Observer's Assessment of Alertness and Sedation), on conscious sedation, physiological stability and recovery time for patients undergoing endoscopic submucosal dissection. Methods: Participants In this study were patients who underwent endoscopic submucosal dissection because of early gastric cancer. Participants were assigned randomly to an experimental group receiving sedatives based on bispectral index monitoring or to a control group with the MOAA/S instrument. Movements, belching, memory, pain, discomfort, physiological stability (MBP, PR, $SpO_2$), and recovery time were measured during the treatment and recovery. Data were analyzed using Spearman partial correlation coefficient analysis, Mixed model and Wilcoxon rank sum test to determine differences in the parameters. Results: There were no statistically significant differences between the two groups for conscious sedation(movement, belching, memory, pain, or discomfort), physiological stability and recovery time. Conclusion: The results of this study indicate that no differences were found between the two types of monitoring. Thus, use of a bispectral index monitor in clinical practice enabling medical staff to readily assess the conscious sedation of for these patients is expected to be increasingly used as an objective assessment tool for conscious sedation for patient safety.

• The Journal of Engineering Geology
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• v.24 no.4
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• pp.525-534
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• 2014

Environmental problems typically occurring in abandoned mine lands (AML) include: contaminated and acidic surface water and groundwater; stockpiled waste rock and mill tailings; and ground subsidences due to mining operations. This study examines the effectiveness of various geophysical techniques for mapping potential hazard and contaminated zones. Four AML sites with sedimentation contamination problems, acid mine drainage (AMD) channels, ground subsidence, manmade liner leakage, and buried mine tailings, were selected to examine the applicability of various geophysical methods to the identification of the different types of mine hazards. Geophysical results were correlated to borehole data (core samples, well logs, tomographic profiles, etc.) and water sample data (pH, electrical conductivity (EC), and heavy metal contents). Zones of low electrical resistivity (ER) corresponded to areas contaminated by heavy metals, especially contamination by Cu, Pb, and Zn. The main pathways of AMD leachate were successfully mapped using ER methods (low anomaly peaks), self-potential (SP) curves (negative peaks), and ground penetrating radar (GPR) at shallow penetration depths. Mine cavities were well located based on composite interpretations of ER, seismic tomography, and well-log records; mine cavity locations were also observed in drill core data and using borehole image processing systems (BIPS). Damaged zones in buried manmade liners (used to block descending leachate) were precisely detected by ER mapping, and buried rock waste and tailings piles were characterized by low-velocity zones in seismic refraction data and high-resistivity zones in the ER data.

• The Journal of Internal Korean Medicine
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• v.38 no.5
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• pp.628-640
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• 2017

Purpose: To describe the effectiveness of traditional Korean medicine (TKM) on the patients with secondary dementia (case 1: Rt. MCA infarction; case 2: traumatic subacute subdural hemorrhage and traumatic intracranial hemorrhage; case 3: delayed encephalopathy after carbon monoxide poisoning). Methods: We used a complete TKM treatment. The acupoints used were as follows: acupoints frequently used in hemiparesis (GV20, GV24, ST4, LI11, LI14, ST36, GB34, SP9, and LR3), and we used herbal medicine (補腎益腦湯加味, Boshiniknoe-tang-gami). The efficacy was evaluated using the Mini-Mental State Examination for Dementia Screening (MMSE-DS) and the Modified Barthel Index (MBI). Results: After treatment, the total MMSE-DS score was increased from 14 to 24 (case 1), 14 to 19 (case 2), and 0 to 17 (case 3). The total K-MBI was increased from 30 to 44 (case 1), 82 to 86 (case 2), and 30 to 86 (case 3) Conclusions: We suggest that TKM could positively influence cognition and improve activity of daily living (ADL). This study has limitations, so further studies are needed.

• The Journal of Korean Society of Virology
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• v.29 no.2
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• pp.129-136
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• 1999

Transient transfection assay has been done to evaluate whether the c-jun activation would be prerequisite to the induction of permissiveness against human cytomegalovirus using in vitro cell model in which U937 has been induced to express CD11b and CD14 to become potential monocyte/macrophage cells by TPA treatment. U937 cells were treated with $10\;{\mu}M$, $50\;{\mu}M$ or $100\;{\mu}M$ of TPA. The cell morphology change was observed and the expression of the CD11b and CD14 was confirmed by FACS. Differentiated cells were transfected with pJLuc reporter vector which contained the wild type murine c-jun promoter spanning the SP1, CTF, ATF/CREB and MEF-2 binding sites upstream of the firefly luciferase gene. After 48 hrs of transfection, the cells were infected with HCMV Towne strain and the luciferase activity was assessed at 1 hand 4 h pi. The transfection assay showed no activation of the c-jun promoter at 1 h pi, instead, it showed 2 times increase of the its activity at 4 h pi. There was no difference of the c-jun promoter activation between TPA treated and untreated U937 cells, implying that c-jun activation might not be prerequisite for allowing cells to be premissive to HCMV, although HCMV infection itself could activate c-jun promoter.

• Korean Journal of Ecology and Environment
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• v.53 no.1
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• pp.46-54
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• 2020

Artificial and natural changes such as weir construction and climate change often cause abnormal blooming of organism. Therefore, variations in species community of organisms have been actively investigated to identify influential environmental changes on the fresh water ecosystem. In this study, we investigated Chironomidae community and environmental factors at 5 representative weirs (Ipo, IP; Sejong, SJ; Juksan, JS; Gangjeong-goryung, GG; and Dalsung weir) in 4 Korean major rivers to figure out relationship between Chironomidae community and environmental factors. Environmental factors indicating organic matter (total organic carbon, TOC and Chlorophyll-a, Chl-a) showed lower concentration in IP and SJ compared with other sites(JS, GG and DS). 3 sub-family 18 genus 25 species of Chironomidae community were found in this study. Among them, Chironominae was dominant in JS (Tanytarsus sp.1), GG (Polypedilum scalaenum) and DS (Polypedilum scalaenum), while different sub-family were dominant in IP (Orthcladinae, Tokunagayusurika akamushi) and SJ (Tanypodinae, Tanypus punctipennis). Moreover, based on the dominant species of Chironomidae community and environmental factors, the cluster analysis classified our study sites into 3 groups. These results imply that the diet resource is the most important factor for dominance of Chironomidae in Korean rivers. We also suggest that further study on the identification of diet resources for each Chironomidae specie is required for better understating of distribution in species community of Chironomidae at various ecosystems.

• Childhood Kidney Diseases
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• v.12 no.2
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• pp.150-156
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• 2008

Purpose : Many results have reported a correlation between the spot urine protein/creatinine ratio(P/C ratio) and 24-hour urinary protein(24UP) amount. This study was designed to evaluated correlation between 24UP amounts and P/C ratio in children and to find the factors that affect this correlation. Methods : 210 patients who visited the Department of Pediatrics in Busan Paik Hospital from september 2003 to december 2007 were included in this study. All the patients were divided into I, II, III/A, B, C group[I:24UP(mg/$m^2$/day)]<100, II: 100$\leq$24UP<1,000, III: 24UP$\geq$1,000, A: Cr excretion(mg/kg)<15, B: 15$\leq$Cr excretion<25, C: Cr excretion$\geq$25)]. Pearson correlation analysis was performed between 24UP and P/C ratio to evaluate the relationship. We defined fractional difference between 24UP and P/C ratio, and then performed multiple regression analysis. Results : There was a strong positive linear correlation between 24UP and P/C ratio in all patients, and the correlation was also good in each group. The factors affecting accurate quantitation of proteinuria using spot urine P/C ratio was creatinine excretion. Conclusion : Spot urine P/C ratio is a useful test to predict proteinuria roughly. Therefore, we expect that urine P/C ratio can be used as parameter instead of 24UP, if we set cutoff value of P/C ratio considered to creatinine excretion according to age and sex in large pediatric population.

• Research in Plant Disease
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• v.11 no.2
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• pp.185-192
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• 2005

In 1999 and 2002, 130 and 258 isolates of Colletotrichum spp. causing red pepper anthracnose were obtained from infected red pepper fruits, respectively. Their responses to 4 protective and 3 ergosterol biosynthesis-inhibiting(EBI) fungicides were investigated by observing their mycelial growth on PDA incorporated with different concentrations of each fungicide. The Colletotrichum isolates obtained in 1999 showed higher $EC_{50}$ values than those isolated in 2002 against three protective fungicides such as dithianon, chlorothalonil, and propineb, whereas the response was reversed toward other protective fungicide, iminoctadine. On the other hand, the isolates of year 1999 were more resistant against three EBI fungicides such as tebuconazole, hexaconazole, and prochloraz than those of year 2002; the $EC_{50}$ values of the former were 1.2-4.4 times higher than those of the latter, The responses of the Colletotrichum isolates toward protective and EBI fungicides were fluctuated according to regions, where the infected fruits were collected. On the other hand, the resis tance of Colletotrichum isolates to protective fungicides increased during monitoring from July to September, However, their responses towards EBI fungicides were not changed.

• Research in Plant Disease
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• v.13 no.2
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• pp.104-109
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• 2007

Pink root disease of onion, a known worldwide constraint upon onion production, significantly reduces crop levels in the main Korea cultivation area. In order to examine the effect of flooding on incidence of pink root disease caused by Pyrenochaeta terrestris and on onion growth and on populations of soil fungi, field experiments were conducted during two seasons, 2003/04 and 2004/05 at Onion Research Institute. Populations of soil fungi from fields were assayed on selective media. Flooding treatment was effective in reducing populations of P. terrestris, Pythium spp., and Rhizoctonia sp. in soil; fungal populations in soils flooded for 90 days were reduced to 1/2 to 1/3 of those in non-flooded soils. In nursery bed, protective activities of soils flooded for over 60 days were 93.5 to 99.2% and their pink root incidences were less than 5%, which were 1/11 to 1/18 of that in control. Increased yield of onion bulbs was associated with control of soil borne pathogenic fungi by flooding treatments. As flooding period was prolonged, bulb grade showed the tendency to increase. Soil flooding for over 60 days resulted in effective disease control, facilitated accurate planning of plant population in the field, extension of the growing-season and consequently higher yields of better quality.