• 청정기술
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• 제26권3호
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• pp.177-185
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• 2020

본 연구에서는 석탄화력발전소에서 전량 폐기되고 있는 석탄재의 재활용 방안으로 석탄회의 80%를 차지하고 있는 비산재로 부터 유용성분을 회수하고 이를 산업재로 활용하기 위한 청정부유선별 공정을 개발하였다. 비산재로부터 미연탄소(unburned carbon, UC) 를 회수하기 위해 비이온성 포수제인 등유 대신에 친환경 식물성 오일인 대두유를 사용하여 등유로부터 악취 발생을 예방하였고 부유선별 후 잔류물로부터 CM (ceramic microsphere)과 CA (cleaned ash)를 분리하기 위해 황산용액을 사용하지 않고 hydro-cyclone를 사용함으로써 산성폐수를 발생시키지 않고 미립의 CM를 회수할 수 있었다. 등유를 포수제로 사용하여 UC를 분리할 때 보다 대두유를 포수제로 사용하였을 때, 대두유의 높은 점성으로 인한 UC의 흡착성 증가와 대두유에 포함된 리놀레산에 의해 부유성 향상으로 UC의 회수율이 85.8%로 높게 나타났다. 회수된 UC에 포함된 연소가능성분(combustible component, CC)은 모두 탄소성분으로 대두유를 사용하였을 때 탄소의 함량이 높게 나타났으며, 회수된 UC는 표면이 거칠면서 기공이 많아 분쇄가 쉬워 미립화로 산업용 소재로 활용할 수 있을 것이다. Hydro-cyclone을 이용한 입도선별 청정분리공정에 의해 회수된 CM과 CA는 구형 형상으로 입자들이 서로 뭉치지 않고 뚜렷하게 분리되었으며 입자의 평균직경(D₅₀)은 5 ㎛로 미세하여 공정변경에 의한 CM의 미립화를 구현할 수 있었다.

• The Plant Pathology Journal
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• 제20권4호
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• pp.252-257
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• 2004

We developed a polyclonal antibody based-ELISA system to monitor inocula accurately and rapidly before onset of anthracnose on soybean sprouts. Titer of mouse antisera against conidia of Colletotrichum gloeosporioides, determined by indirect ELISA, was high enough to be detectable up to ${\times}$25,600 dilutions. Both PAb1 and PAb2 had the highest level of reactivity to Colletotrichum gloeosporioides. Absorbance readings exceeded 0.15. Sensitivity of PAb to C. gloeosporioides was precise enough to detect spore concentration as low as 500 conidia/well by indirect ELISA. Both antibodies are very sensitive and highly specific to the target pathogen Colletotrichum gloeosporioides, apparently discriminating other unrelated pathogen, or epiphytes. This kit fulfills the requirements far detecting inocula before infection and onset of anthracnose. Our ELISA system should also be feasible to detect C. acutatum (Mungbean sprouts rot) and G. cingulata (C. gleosporioides), (apple, pepper). It was remarkable that absorbance value was not reduced even after 4 consecutive washings (Fig.4), suggesting that antigenic determinants are on the surface of conidia. Antigenic determinant was characterized by heating and enzyme treatment: Both PAb1 and PAb2 bind to protein epitope that does not contain residue of amino acid, arginine, and Iysine, even though more work needs to be done.

• Journal of Nutrition and Health
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• 제36권5호
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• pp.425-436
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• 2003

The present study was conducted to evaluate the usefulness of common Korean legumes as a high-fiber supplement in therapeutic diets for diabetic patients. Streptozotocin-induced diabetic rats were used as animal models and four kinds of legumes, black soybean (BS), yellow soybean (YS), green pea (GP) and soybean curd residue (SCR) were tested as high-fiber supplements. Seven groups of normal and streptozotocin-induced diabetic rats were fed isocaloric experimental diets containing 8％ dietary fiber from one of four legumes or purified cellulose and pectin for 6 weeks. The effects of the legumes on the glucose and lipid metabolism of diabetic rats was examined and compared with the effects of cellulose and pectin. The legume supplementation did not show any beneficial effect on glucose tolerance, however, it exhibited a plasma cholesterol-lowering effect in diabetic rats. The cholesterol-lowering action was especially strong in BS and the degree of the effect was comparable to that of pectin. The levels of total lipids, cholesterol, and triglyceride in the hepatic tissues of rats fed legume diets were similar to those of the pectin group. All legume supplements induced an increase in fecal steroid excretion. The fecal cholesterol contents were significantly high following the supplementations of YS and SCR (p < 0.05). The excretion of fecal bile acids in the BS and YS groups was significantly higher than it was in the pectin group (p < 0.05). Concentration of lipid peroxidation products in the blood and urine of diabetic animals was lower in the legume groups than in the cellulose group. The levels of hepatic lipid peroxidation products were significantly lower in the BS and YS groups than in the pectin group (p < 0.05). From the results of this study, the plasma cholesterol-lowering effect of BS is possibly due to the significant (p < 0.05) in-crease in fecal steroid excretion, which suggests that BS could be beneficial in improving abnormal lipid metabolism in diabetic rats. (Korean J Nutrition 36(5): 425∼436, 2003)

• Preventive Nutrition and Food Science
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• 제9권2호
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• pp.138-143
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• 2004

Soybean curd residues (SCR) obtained from hot and cold manufacturing processes were fermented by indigenous microorganisms, Lactobacillus rhamnosus LS and Bacillus firmus NA-l for 15 h at 37$^{\circ}C$. The pH, acidity, viable cell counts, and tyrosine content were evaluated in samples with variations in sugar, starter and type of SCR. The raw Doowon SCR (D-SCR, cold-processed) fermented by indigenous microorganism had a 0.9% acidity and 6.7 ${\times}$ 10$^{7}$ CFU/g viable cell counts, compared with the 0.11 % acidity and 6.7 ${\times}$ 10$^{6}$ CFU/g viable cell counts of raw fermented Pulmuwon SCR (P-SCR, hot-processed). After fermentation of raw P-SCR with 1 % glucose and 1 % L. rhamnosus LS starter, the viable cell counts, tyrosine content and acidity were 4.7 ${\times}$ 10$^{8}$ CFU/g, 16.3 mg% and 0.9%, respectively. In addition, the raw P-SCR fermented with Bacillus firmus NA-l as co-starter had a 0.45% acidity, 2.4 ${\times}$ 10$^{8}$ CFU/g lactic acid bacteria, and 3.3 ${\times}$ 10$^{6}$ CFU/g Bacillus sp. In particular, the tyrosine content was increased 5 fold. The drying of fermented SCR was completed by hot-air drying (5$0^{\circ}C$) within 12 h; the dried P-SCR and D-SCR had 1.8 ${\times}$ 10$^{7}$ CFU/g and 5.3 ${\times}$ 10$^{6}$ CFU/g viable cell counts, respectively. The concentrate of methanol extract from fermented D-SCR inhibited the initial cell growth of E. coli, Staphylococcus aureus and Pseudomonas aeruginosa in liquid culture.

• Journal of Ginseng Research
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• 제40권3호
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• pp.292-299
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• 2016

Background: We previously reported that two-phase partition chromatography between ginseng water extract and soybean oil efficiently eliminated pesticide residues. However, an undesirable odor and an unpalatable taste unique to soybean oil were two major disadvantages of the method. This study was carried out to find an alternative vegetable oil that is cost effective, labor effective, and efficient without leaving an undesirable taste and smell. Methods: We employed six vegetable oils that were available at a grocery store. A 1-mL sample of the corresponding oil containing a total of 32 pesticides, representing four categories, was mixed with 10% aqueous ginseng extract (20 mL) and equivalent vegetable oil (7 mL) in Falcon tubes. The final concentration of the pesticides in the mixture (28 mL) was adjusted to approximately 2 ppm. In addition, pesticides for spiking were clustered depending on the analytical equipment (GC/HPLC), detection mode (electron capture detector/nitrogen-phosphorus detector), or retention time used. Samples were harvested and subjected to quantitative analysis of the pesticides. Results: Soybean oil demonstrated the highest efficiency in partitioning pesticide residues in the ginseng extract to the oil phase. However, canola oil gave the best result in an organoleptic test due to the lack of undesirable odor and unpalatable taste. Furthermore, the qualitative and quantitative changes of ginsenosides evaluated by TLC and HPLC, respectively, revealed no notable change before or after canola oil treatment. Conclusion: We suggest that canola oil is an excellent vehicle with respect to its organoleptic property, cost-effectiveness and efficiency of eliminating pesticide residues in ginseng extract.

• 한국식품영양과학회지
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• 제29권5호
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• pp.948-956
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• 2000

There is growing evidence that soy isoflavone play beneficial roles in the prevention of chronic diseases such as hormone dependent cancers, cardiovascular diseases, osteoporosis and also reduced incidence of menopausal symptoms. However current data are not sufficient to determine the effective doses for beneficial as well as harmful effects and to support dietary recommendation for isoflavones. Since soy products containing isoflavone are one of the common food items that Koreans consume daily basis, assessing consumption of soy isoflavone by Koreans will give a valuable information on making dietary recommendations for isoflavones. the present study was designed to assess dietary intake of isoflavone of Korean middle-aged women who might receive the most beneficial effects from isoflavone consumption thereby prevent post menopausal related symptoms and diseases. In this study isoflavone contents of soy products were analyzed and soy products and isoflavone consumption of these subjects were assessed by a self-reported dietary questionnaire and isoflavone intake data base. Subjects were consisted of 178 Korean women (35~60 y) who live in urban and rural area. All subjects provided detailed information on demographics, anthropometry, health history, menopausal symptoms, health history, menopausal symptoms, and dietary intake using food frequency questionnaire (FFQ) which was developed for Koreans. The average age of the subjects was 46.6 years. The soy products which the subjects frequently consumed were soybean paste soup, braised fried soybean, tofu residue stew, tofu, soy milk, natto stew, bean sprouts, and soybean broth which contained 10.68 mg, 3.34 mg, 2.44 mg, 2.42 mg, 2.42 mg, 1.12 mg, 1.02 mg, 0.33 mg of isoflavone per 100 g, respectively. The estimated daily intake (EDI) of isoflavones ranged from 0 to 144.3 mg, and the mean daily isoflavone (sum of daidzein and genisten) intake of the subjects was 24.41 mg.

• 한국작물학회지
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• 제66권4호
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• pp.358-364
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• 2021

본 연구에서는 콩 수확 효율 증진을 위한 건조제를 선발하기 위해 2019년부터 2020년까지 2년간 4종의 비선택성 제초제를 활용하여 건조제의 선발시험 및 지상부 건조와 약해 시험을 수행하였다. 1. 콩 수확기 건조제로 사용한 4종의 비선택성 제초제 모두 처리시기가 빠를수록 건조효과를 보이는 것으로 확인되었으나, 통계적인 차이를 보이지 않았다. 2년차 실험에서도 처리시기에 따른 건조효과를 확인하였으나, 통계적인 차이는 지역간 차이를 보였다. 이는 건조제가 콩 성숙기 전후에 처리하여 수확을 하였는데, 보통 성숙기 약 1~2주후면 예취 수확이 가능한 상태로 콩 잎이 대부분 떨어져 있으며, 줄기도 일정 부분 건조되어 있기 때문으로 무처리에서도 일정 수준 이상의 건조가 이루어지기 때문으로 보인다. 2. 건조제 처리에 따른 수확량의 감소는 보이지 않았으며, 100립중, 종실의 모양과 색변화, 수확된 종자의 발아율의 저하가 확인 되지 않아 약해가 없는 것으로 확인되었다. 3. 건조제 처리에 따른 종실 내 잔류농약은 처리시기가 빠를수록 검출량이 증가하였으며, 시험약제 중 글루포시네이트암모늄의 검출량이 높았으며, 티아페나실이 보다 안전한 것을 확인하였다. 다만 콩 잔류농약허용치 이하의 값을 나타내 적용은 가능한 것으로 판단되었다. 4. 본 연구는 콩 건조제 처리로 기계수확을 1~2주 정도 단축시킬 것으로 보여 작부체계의 선택 폭을 넓히는데 기여할 수 있는 것으로 판단된다.

• Preventive Nutrition and Food Science
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• 제5권3호
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• pp.160-164
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• 2000

The content of total dietary fiber in biji dried by hot air was 65.40%, and the percent of insoluble fiber and soluble fiber in total dietary fiber was 63.60% and 1.80%, respectively. In testing the feasibility of biji as a fiber source in cake, cakes were prepared with flour substituted with biji powder at the level of 0%, 5%, 10%, 15% and 20%. The Hunter value showed that the crumb colors of the cakes with 10%, 15% and 20% biji powder were significantly different from the control. The specific volume of the cake with 20% biji powder was significantly lower tan the other cakes. Cakes with 5%, 10% and 15% biji powder were les hard than the control after 10 days storage at 2$0^{\circ}C$. Sensory evaluations showed that their characteristics - crumb color, crumb texture, moistness, softness and overall preference - were significantly influenced by the replacement of flour with 10%, 15% and 20% biji powder. However, there was no difference in softness and overall preference of the cake prepared with 5% replacement compared to the control.

• Food Science and Biotechnology
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• 제18권6호
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• pp.1519-1522
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• 2009

This study reports the use of different inducing agents (olive, soybean, and used frying oils) and culture mediums [synthetic medium (SM), whey protein, and corn steep liqueur (SL)] to optimize the production of lipase by Fusarium oxysporum. A relationship among the inoculum size, presence of a fat source, fungal growth, and lipase production was evident during the fermentation. The best results were achieved when the inoculum was grown in SM or SL and the fermentation was developed in SM with frying oil as the inducing agent. The maximum activity (about 15 U/mL) was obtained after a 72 hr cultivation.

• 한국식품과학회지
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• 제30권6호
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• pp.1327-1332
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• 1998

The optimum thermal condition of retort Biji product was determined by heat penetration curve, aerobic bacteria count and sensory test. Retort Biji showed a simple logarithmic heating curve regardless of solid content. Heating time was a $26{\sim}27$ min until Fo value reached 9 min and the amount of microorganism in the Biji product sterilized for 26 min at $121^{\circ}C$ were decreased to $10^{-4}\;CFU/g$, indicating the safe range for retort product. The rate of heat penetration was reduced as solid content and size of product were increased, whereas sterilization temperature and initial temperature of product influenced the heat penetration curve. Sensory scare indicated that there was no significant difference in color, flavor, and appearance among different thermal processes. However, Biji product sterilized at $121^{\circ}C$ showed the highest score in overall preference value.