• The Plant Pathology Journal
• /
• v.25 no.4
• /
• pp.381-388
• /
• 2009

On screening pathogen-resistant soybean, we identified a WRKY type transcription factor named a Glycine max WRKY1 (GmWRKY1). Expression of GmWRKY1 gene was induced in the soybean sprout by Pseudomonas infection. The GmWRKY1 was expressed in all of the tissues with high levels in stems, leaves and developing seeds. The protein Gm WRKY1 contains highly conserved two WRKY DNA-binding domains having two $C_2-H_2$ zinc-finger motif ($C-X_{4-5}-C-X_{22-23}-H-X-H$) in its N-terminal and C-terminal amino acid sequences. In electrophoresis mobility shift assay, the GmWRKY1 protein bound specifically to W-box elements in the promoters of defense related genes. These results demonstrated that GmWRKY1 is one of the soybean WRKY family genes and the plant-specific transcription factors for defense processes.

• The Plant Pathology Journal
• /
• v.40 no.3
• /
• pp.322-328
• /
• 2024

Soybean (Glycine max), a crucial global crop, experiences yearly yield reduction due to diseases such as anthracnose (Colletotrichum truncatum) and root rot (Fusarium spp.). The use of fungicides, which have traditionally been employed to control these phytopathogens, is now facing challenges due to the emergence of fungicide-resistant strains. Streptomyces bacillaris S8 strain S8 is previously known to produce valinomycin t through a nonribosomal peptide synthetase (NRPS) pathway. The objective of this study was to evaluate the antifungal activity of S. bacillaris S8 against C. truncatum and Fusarium sp., assessing its efficacy against soybean pathogens. The results indicate that strain S8 effectively controlled both above-ground and underground soybean diseases, using the NRPS and NRPS-related compound, suggesting its potential as a biological control in plant-microbe interactions. These findings underscore the pivotal role of the stain S8 in fostering healthy soybean microbial communities and emphasize the significance of microbiota structure studies in unveiling potent biocontrol agents.

• The Plant Pathology Journal
• /
• v.15 no.1
• /
• pp.1-7
• /
• 1999

The production of soybean cyst nematode (SCN), Heterodera glycines, including female formation and fecundity was much higher in SCN race 14 (R14) than in race 3 (R3) in susceptible soybean cultivars Bragg (intolerant), Lee74 (moderately tolerant), and PI 97100 (highly tolerant). The nematode body was also significantly larger in R14 than in R3 at 20 days after inoculation, but the further nematode growth appeared to be slower in R14 than in R3, resulting in no significant difference between the two races at 30 days after inoculation. Within each race, no significant difference was observed in the growth and reproduction among the soybean cultivars tested. Syncytial areas near the nematode lip regions (infection sites) were measured for each soybean cultivar-SCN race combination. R14 induced significantly larger syncytia than R3. Bragg had relatively larger syncytia than Lee74 and PI 97100, but the difference among the soybean cultivars was minimal or not significantly different. Syncytium occupation in the stelar region differed only between PI 97100 and the other two cultivars, which may be somewhat, but not exactly, related to tolerance levels. Syncytial cytomplasm was degenerated more with R14 and in Bragg than with R3 and in Lee74 and PI 97100, respectively. In light microscopy, degenerated syncytia were characterized by depleted and loose cytoplasm with less plastids than normal-looking (intact) syncytia which had dense syncytial cytoplasm. Electron microscopy revealed that degenerated syncytia contained highly vacuolated cytoplasm with degenerated plastids. The above results suggest that structural characteristics of syncytia may match the nematode growth and reproduction.

• The Plant Pathology Journal
• /
• v.26 no.2
• /
• pp.154-158
• /
• 2010

Early nematode development and subcellular responses in resistant soybean lines PI 88788 and PI 437654 infected with races 3 (R3) and 14 (R14) of soybean cyst nematode (SCN), Heterodera glycines Ichinohe, were compared. SCN R14 nematodes penetrated and developed significantly more than R3 at 5-6 days after inoculation. Both races also penetrated and developed more in PI 88788 than in PI 437654. Syncytia, characterized by cell wall dissolution and cellular hypertrophy, were developed more in PI 88788 than in PI 437654 and more by R14 than R3, for which less necrotic responses occurred in the former than the latter. This suggests that the latter two may be more resistant and less virulent than the former two, respectively. A common structural feature found in each of PI 437654 and PI 88788 in relation to SCN-resistance was the formation of prominent cell wall appositions and nuclear degeneration prior to cytoplasmic degradation in syncytial cells, respectively. Necrosis and cell wall apposition are types of hypersensitive responses occurring at early stages of the nematode infection so that these structural modifications indicate the inhibition of initial syncytial development related to the early nematode development. As soybean cultivars and lines with identical or similar genotypes have the same types of structural features related to SCN-resistance, the structural modifications induced by SCN infection may result from the expression of inheritable resistance genes, of which the information can be used for breeding soybean cultivars and lines specifically resistant to SCN races.

• Korean Journal of Agricultural Science
• /
• v.20 no.2
• /
• pp.117-124
• /
• 1993

This study was conducted to determine the effects of alternating planting systems of soybean and tillering hybrid corn on yield of both crops. The planting systems included monocultures of both crops. 1 to 1 row ratio, 1 to 2 row ratio, 1 to 3 row ratio, 2 to 1 row ratio, 2 to 3 row ratio, 3 to 1 row ratio, 3 to 2 row ratio and 3 to 3 row ratio of soybean and corn. The results obtained are summarized as follows : The plant height of soybean was increased as the number of planting rows of corn increased. But the plant, height of soybean was quite stabilized when the ratio of soybean planting rows increased. When the plant height of soybean was increased due to the increased row number of corn, the branch number and diameter of soybean decreased and lodging ratio increased. Total fresh weight of soybean per 10a was decreased as the ratio of soybean planting decreased and it was increased when the ratio of soybean planting increased over monoculture of soybean. Dry weight of soybean per 10a showed the same tendency of the fresh weight. But the highest grain yield of soybean was obtained in the monoculture of soybean and it was 276 kg/10a. Comparatively high yield of soybean was obtained in the systems of 2 to 1 and 3 to 3 ratios. The plant height, number of tillers and number of ears per plant of tillering corn were greatly affected by the alternating planting of soybean and they were 208.9cm, 2.1 and 4 per plant, respectively. The fresh and dry weights and yield of corn per 10a showed significant differences among systems. The average fresh and dry weight of corn in the systems of 3 to 1 and 2 to 1 were 1000 kg and 100 kg higher than those of monoculture of corn. The kernel yields of corn per 10a were 438 kg in the monoculture system and 530 kg in the systems of 3 to 2 and 2 to 1. The total fresh weights of corn and soybean in systems of 3 to 1, 3 to 2 and 2 to 1 were higher than 5721 kg of soybean and 4358 kg of corn in the monoculture. Combined dry weight was high in the order of monoculture, 2 to 1 and 3 to 1 row ratios. Combined grain yield was high in the systems of 2 to 1 and 3 to 1 row ratios and it was over 430 kg per 10a.

• KOREAN JOURNAL OF CROP SCIENCE
• /
• v.41 no.1
• /
• pp.20-27
• /
• 1996

Greenhouse experiment was carried out to investigate the specifics in growth, weed competition, and herbicide response of soybean(Glycine max) under foggy condition. Plant height and leaf stage of soybean under foggy condition were greatly increased through all growing stage comparing with non-foggy condition. At 100 days after seeding, plant height of soybean also was increased by 14％ while number of pods, number of grains per pod were reduced by 16, 24 and 23％, respectively comparing with non-foggy condition. On the other hand, plant height of soybean under foggy condition was reduced with increasing the duration of competition and this tendency was similar to non-foggy condition. Plant height was reduced by above 50-day competition. Number of pods per plant of soybean was reduced regardless of foggy condition. Among the herbicides treated, quizalofop caused slightly crop injury under foggy condition, but the other herbicides did not affect growth of soybean. Number of pods and 100-grain weight of soybean by treatment of herbicides were similar to that of the untreated control but those of soybean under foggy condition were significantly reduced. At 40 days after herbicide application, weeding efficacy (based on shoot fresh weight) of herbicides treated under foggy and non-foggy condition, released exellent data(above than 97％) in all regardless foggy condition.

• KOREAN JOURNAL OF CROP SCIENCE
• /
• v.7 no.1
• /
• pp.133-137
• /
• 1969

This experiment was conducted to determine the optimum row width and plant spacing within row under the tropical environment for a leading soybean variety Palmetto and it was carried out at Eakmat Experiment Station in Vietnam. The experiments were arranged in a split plot design with four replications and the test was repeated twice in two years. Variations for seed yield due to the distance between rows were significant at 1 per cent level in 1967 test(dry season growing) and at 5 per cent level in 1968 test. Significant differences for plant height, lodging, maturity, number of branches per plant, and number of pods per plant due to the row width were not found in both tests, while significant differences in difference spacing within row was found in all traits studied. Interaction between width of row and spacing within row for seed yield and plant height were found at five percent level in 1967 test. These results indicate that close planting may increase in seed yield and plant height in both seasons, and decrease in loadging. From these studies, one could be understood that the plant population, particularly in dry season, plays decisive roles on seed yield in soybean culture, and the maximum plant height and minimum value of lodging index were also observed in closest spacing plots. The highest soybean yield in late planting would be expected by a combinations of 30 cm(between rows)${\times}$5cm(between hills) plot, while 40cm ${\times}$ 5cm planting method would be suggested for rainy season growing. Highest seed yield was obtained at closet spacing in both dry and rainy season, and these results led to drilling method in seeding where about 20 seeds per meter of row could be recommended. Besides the seed yield, the close planting may produce some advantages, such as increase plant height and decrease lodging, weeds and erosion of surface soil.

• KOREAN JOURNAL OF CROP SCIENCE
• /
• v.36 no.3
• /
• pp.220-225
• /
• 1991

Four cropping systems, soybean mono-cropping, Cassia tora mono-cropping, soybean-C. tora companion cropping and soybean-C. tora intercropping, were compared for number of soil nematodes and soybean growth in upland field in 1986. Soybean was cultivated as an after-crop of four different cropping systems from 1987 to 1989, and observations were continued. Soil nematode was not found in C. tora mono-cropping plot from mid growing season of C. tora while many nematodes were found in soybean mono-cropping plot in 1986. A few nematodes were found for 3 years in soybean field after one season cultivation of C. tora in 1986. Number of nematodes was lower in soybean-C. tora companion and inter-cropping plots compared with soybean mono-cropping, and was also lower for 3 years in soybean field after one season companion and inter-cropping culture in 1986 compared with continuous soybean cultivation plot. No root nodule was formed on C. tora root, but number of root nodules of soybean plant grown in C. tora companion and inter-cropping plots was not different with soybean mono-cropping plot. Number of pods. number of seeds and seed weight per soybean plant grown in C. tora companion and inter-cropping plots were almost same with soybean mono-cropping plot. When soybean was cultivated in field after one season C. tora mono, companion or inter-cropping, pod and seed number, and seed weight per plant were higher than continuous soybean cultivation field.

• KOREAN JOURNAL OF CROP SCIENCE
• /
• v.48 no.2
• /
• pp.96-102
• /
• 2003

This study was conducted to determine the effects of two plant populations (28 and 14 plants per $m^2$) and two toppings in conventional plant population (28 plants per $m^2$) on soybean (Glycine max L. cv. Pungsannamulkong) cultivated in the paddy field. The two topping time were taken at 6$^{th}$ to 7$^{th}$ and 8$^{th}$ to 9$^{th}$ trifoliolate leaf stages in the conventional plant population. Experimental design for growth data was a randomized complete block with three replications, and samples were taken at R1 (July 31), R3 (August 19), R5 (September 2) and R7 (September 23) growth stages. The branch number of soybean was relatively higher in the low plant population (14 plants per $m^2$) and with the topping at the 6$^{th}$ to 7$^{th}$ leaf stage, in the conventional plant population (28 plants per $m^2$), and with topping at the 8$^{th}$ to 9$^{th}$ trifoliolate leaf stage in descending order. The highest average branch length of soybean was observed in the low population and the longest branch length was observed from the soybean with topping at the 6$^{th}$ to 7$^{th}$ leaf stage. The leaf number per plant was decreased in order of in the low population, with the topping at 6$^{th}$ to 7$^{th}$ trifoliolate leaf stage, with the topping at 8$^{th}$ to 9$^{th}$ trifoliolate leaf stage, and in the conventional population. The leaf area was high in the low population and with topping at 6$^{th}$ to 7$^{th}$ trifoliolate leaf stage and was relatively low in the conventional population and with the topping at 8$^{th}$ to 9$^{th}$ trifoliolate leaf stage in soybean. The dry weight of leaves and branches was high in the low population and with the topping at 6$^{th}$ to 7$^{th}$ trifoliolate leaf stage and was relatively low in the conventional population and with topping at 8$^{th}$ to 9$^{th}$ trifoliolate leaf stage. The leaf number per plant was high in the low population and with topping at 6$^{th}$ to 7$^{th}$ trifoliolate leaf stage and was relatively low in the conventional population and with topping at 8$^{th}$ to 9$^{th}$ trifoliolate leaf stage. The grain yield per 10a was high with the topping at 6$^{th}$ to 7$^{th}$ trifoliolate leaf stage.

• Proceedings of the Korean Society of Plant Pathology Conference
• /
• 2005.04a
• /
• pp.58.2-59
• /
• 2005