Browse > Article
http://dx.doi.org/10.5423/PPJ.2009.25.4.381

Identification of a Pathogen-Induced Glycine max Transcription Factor GmWRKY1  

Kang, Sang-Gu (Department of Biotechnology, School of Biotechnology, Yeungnam University)
Park, Eui-Ho (Department of Biotechnology, School of Biotechnology, Yeungnam University)
Do, Kum-Sook (Department of Biotechnology, School of Biotechnology, Yeungnam University)
Publication Information
The Plant Pathology Journal / v.25, no.4, 2009 , pp. 381-388 More about this Journal
Abstract
On screening pathogen-resistant soybean, we identified a WRKY type transcription factor named a Glycine max WRKY1 (GmWRKY1). Expression of GmWRKY1 gene was induced in the soybean sprout by Pseudomonas infection. The GmWRKY1 was expressed in all of the tissues with high levels in stems, leaves and developing seeds. The protein Gm WRKY1 contains highly conserved two WRKY DNA-binding domains having two $C_2-H_2$ zinc-finger motif ($C-X_{4-5}-C-X_{22-23}-H-X-H$) in its N-terminal and C-terminal amino acid sequences. In electrophoresis mobility shift assay, the GmWRKY1 protein bound specifically to W-box elements in the promoters of defense related genes. These results demonstrated that GmWRKY1 is one of the soybean WRKY family genes and the plant-specific transcription factors for defense processes.
Keywords
soybean; WRKY; W-box element; zinc-finger protein;
Citations & Related Records
Times Cited By KSCI : 2  (Citation Analysis)
Times Cited By Web Of Science : 0  (Related Records In Web of Science)
연도 인용수 순위
1 Asai, T., Tena, G., Plotnikova, J., Willmann, M. R., Chiu, W. L., Gomez-Gomez, L., Boller, T., Ausubel, F. M. and Sheen, J. 2002. MAP kinase signalling cascade in Arabidopsis innate immunity. Nature 415:977-983   DOI   ScienceOn
2 Chen, C. and Chen, Z. 2002. Potentiation of developmentally regulated plant defense response by AtWRKY18, a pathogen-induced Arabidopsis transcription factor. Plant Physiol. 129:706-716   DOI   ScienceOn
3 Ciolkowski, I., Wanke, D., Birkenbihl, R. P. and Somssich, I. E. 2008. Studies on DNA-binding selectivity of WRKY transcription factors lend structural clues into WRKY-domain function. Plant Mol. Biol. 68:81-92   DOI   PUBMED
4 Kang, S. G, Cha, H. W., Chang, M. U. and Park, E. 2003. ldentification of differentially displayed genes of a Pseudomonas resistant soybean (Glycine max). Plant Pathol. J. 19:239-247   DOI   ScienceOn
5 Li, J., Brader, G. Kariola, T. and PaIva, E. T. 2006. WRKY70 modulates the selection of signaling pathways in plant defense. Plant J. 46:477-491   DOI   ScienceOn
6 Maeo, K., Hayashi, S., Kojima-Suzuki, H., Morikami, A. and Nakamura, K. 2001. Role of conserved residues of the WRKY domain in the DNA-binding of tobacco WRKY family proteins. Biosci. Biotechnol. Biochem. 65:2428-36   DOI   ScienceOn
7 Yang, P., Wang, Z., Fan, B., Chen, C. and Chen, Z. 1999. A pathogen-and salicylic acid-induced WRKY DNA-binding activity recognizes the elicitor response element of the tobacco class I chitinase gene promoter. Plant J. 18:141-149   DOI   ScienceOn
8 Robatzek, S. and Somssich, I. E. 2001. A new member of the Arabidopsis WRKY transcription factor family, AtWRKY6, is associated with both senescence- and defence-related processes. Plant J. 28:123-33   DOI   ScienceOn
9 Xu, X. P., Chen, C. H., Fan, B. F. and Chen, Z. X. 2006. Physical and functional interactions between pathogen-induced Arabidopsis WRKY18, WRKY40, and WRKY60 transcription factors. Plant Cell 18:1310-1326   DOI   ScienceOn
10 Jones, J. D. and Dangl, J. L. 2006. The plant immune system. Nature. 444:323-329   DOI   ScienceOn
11 Li, J., Brader, G. and Palva, E. T. 2004. The WRKY70 transcription factor: a node of convergence for jasmonate-mediated and salicylate-mediated signals in plant defense. Plant Cell 6:319-331
12 Du, L. and Chen, Z. 2000. Identification of genes encoding receptor-like protein kinases as possible targets of pathogen- and salicylic acid-induced WRKY DNA-binding proteins in Arabidopsis. Plant J. 24:837-847   DOI   ScienceOn
13 Katagiri, F. 2004. A global view of defense gene expression regulation-a highly interconnected signaling network. Curr. Opin. Plant BioI. 7:506-511   DOI   PUBMED   ScienceOn
14 Bostock, R. M. 2005. Signal crosstalk and induced resistance: straddling the line between cost and benefit. Annu. Rev. Phytopathol. 43:545-580   DOI   PUBMED   ScienceOn
15 Wang, D., Weaver N. D., Kesawani, M. and Dong, X. 2005. Induction of protein secretory pathway is required for systemic acquired resistance. Science 308:1036-1040   DOI   PUBMED   ScienceOn
16 Kim, K. C., Fan, B. and Chen, Z. 2006. Pathogen-induced Arabidopsis WRKY7 is a transcriptional repressor and enhances plant susceptibility to Pseudomonas syringae. Plant Physiology 142:1180-1192   DOI   ScienceOn
17 Yang, B., Srivastava, S., Deyholos, M. K. and Kav, N. N. V. 2007. Transcriptional profiling of canola (Brassica napus L.) responses to the fungal pathogen Sclerotinia sclerotiorum. Plant Sci. 173:156-171   DOI   ScienceOn
18 Chen, C. and Chen, Z. 2000. Isolation and characterization of two pathogen- and salicylic acid-induced genes encoding WRKY DNA-binding proteins from tobacco. Plant Mol. Biol. 42:387-396   DOI   ScienceOn
19 Cormack, R. S., Eulgem, T., Rushton, P. J., Kochner, P., Hahlbrock, K. and Somssich, I. E. 2002. Leucine zipper-containing WRKY proteins widen the spectrum of immediate early elicitor-induced WRKY transcription factors in parsley. Biochim. Biophys. Acta. 1576:92-100   DOI   PUBMED   ScienceOn
20 Deslandes, L., Olivier, J., Theulieres, F., Hirsch, J., Feng, D. X., Bittner-Eddy, P., Beynon, J. and Marco, Y. 2002. Resistance to Ralstonia solanacearum in Arabidopsis thaliana is conferred by the recessive RRS1-R gene, a member of a novel family of resistance genes. Proc. Natl. Acad Sci. USA 99:2404-2409   DOI   ScienceOn
21 Yu, D., Chen, C. and Chen, Z. 2001. Evidence for an important role of WRKY DNA binding proteins in the regulation of NPRI gene expression. Plant Cell 13:1527-1540   DOI   ScienceOn
22 Eulgem, T. and Somssich, I. E. 2007. Networks of WRKY transcription factors in defense signaling. Curr. Opin. Plant Biol. 10:366-371   DOI   ScienceOn
23 AbuQamar, S., Chen X., Dhawan, R., Bluhm, B., Salmeron, J., Lam, S., Dietrich, R. A. and Mengiste, T. 2006. Expression profiling and mutant analysis reveals complex regulatory networks involved in Arabidopsis response to Botrytis infection. Plant J. 48:28-44   DOI   ScienceOn
24 Journot-Catalino, N., Somssich, I. E., Roby, D. and Kroj, T. 2006. The transcription factors WRKY11 and WRKY17 act as negative regulators of basal resistance in Arabidopsis thaliana. Plant Cell 18:3289-3302   DOI   ScienceOn
25 Rushton, P. J., Torres, J. T., Parniske, M., Wernert, P., Hahlbrock, K. and Somssich, I. E. 1996. Interaction of elicitor-induced DNA binding proteins with elicitor response elements in the promoters of parsley PR1 genes. EMBO J. 15:5690-5700   PUBMED
26 Eulgem, T, Rushton, P. J., Schmelzer, E., Hahlbrock, K. and Somssich, I. E. 1999. Early nuclear events in plant defense signaling: rapid gene activation by WRKY transcription factors. EMBO J. 18:4689-4699   DOI   ScienceOn
27 Eulgem, T., Rushton, P. J., Robatzek, S. and Somssich, I. E. 2000. The WRKY superfamily of plant transcription factors. Trends in Plant Sci. 5:199-206   DOI   ScienceOn
28 Dong, J. X., Chen, C. H. and Chen, Z. X. 2003. Expression profiles of the Arabidopsis WRKY gene superfamily during plant defense response. Plant Mol. Biol. 51:21-37   DOI   ScienceOn
29 Ulker, B. and Somssich, I. E. 2004. WRKY transcription factors: from DNA binding towards biological function. Curr. Opin. Plant Biol. 7:491-498   DOI   ScienceOn
30 Oh, S.-K., Baek, K.-H., Park, J. M., Yi, S. Y., Yu, S. H., Kamoun, S. and Choi, D. 2008. Capsicum annuum WRKY protein CaWRKY1 is a negative regulator of pathogen defense. New Phytologist. 177:977-987   DOI   ScienceOn
31 Lim, J. S., Do, K. S., Lee, D. S., Kang, S. G, Suh, S. G. and Park, E. 2008. Identifications of a sprout-rot pathogen Pseudomonas species SN239 and selection resistant soybean line. J. of Life Sci. 18:1771-1774   DOI   ScienceOn