• Korean Journal of Soil Science and Fertilizer
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• v.11 no.2
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• pp.105-112
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• 1979

A study was carried out to examine the effects of sulfur dioxide gas on soybean plants; at two concentrations(0.2mg/l and 0.5mg/l) and in different fumigation periods, The grain yield of soybeans, sulfur and chlorophyll contents in leaves, and ratios of destroyed leaf were investigated, 1) At the concentration of $SO_2$ 0.5mg/l, a) The yield decrease were 52% and 76.4% at 5 minutes and one houre fumigation period respectively, b) The sulfur contents in leaves were positively correlated with fumigation period and yield decrease, however the water soluble sulfur was superior than the total sulfur in correlation coefficient. 2) At the concentration of $SO_2$ 0.2mg/l; a) The most severe destruction of leaves were observed at noon exposure (10:00-11:00 O'clock) and no visible damage was observed at night exposure (22:00-23:00) b) The sulfur contents in leaves and stems were the highest at noon exposure and the lowest at evening exposure (18:00-19:00 O'clock) chlorophyll contents were the highest at night exposure but, the lowest at noon exposure, c) The ratio of destroyed leaf was positively correlated with the sulfur content of leaves, but negatively correlated with chlorophyll contents. d) Absorbance spectra of chlorophyll in the chlorolast were greatly decreased at noon exposure but slightly decreased at night exposure. The decrease of absorbance spectra was mainly at $456m{\mu}$ and $663m{\mu}$.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.38 no.2
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• pp.159-165
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• 1993

To clarify the effects of sink demand for assimilate on leaf photosynthetic rate, tissue composition, and leaf senescence of soybean [Glycine max (L.) Merr.] J plants, pod and leaf tissues were removed at growth stage $R^3$. Plant responses were measured every 10days from 2 through 42days following treatment. Leaves of depodded plants exhibited increased starch and chlorophyll contents and specific leaf weight. Stomatal resistance was also increased and leaf photosynthetic rate was reduced. Dry weight of vegetative tissues except leaves was increased by pod removal. Leaf removal resulted in a decreased starch content of leaves from 22 to 42days after treatment and that of roots at all sampling times. Specific leaf weight was decreased while leaf photosynthetic rate was increased. Stomatal resistance and chlorophyll content were little affected. Weight per seed was decreased 3.0% by leaf removal. Except for the seed, tissue protein contents were increased by pod removal but decreased by leaf removal, however, seed protein content was not affected by either. Apparent senescence was delayed by depodding. Both apparent and functional senescence were accelerated by leaf removal.

• Journal of the Korean Society of Food Science and Nutrition
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• v.31 no.3
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• pp.521-526
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• 2002

A number of experimental and epidemiological studies have implicated that antiestrogenic effects of estrogen-like compounds in legumes and plant seeds are responsible for lowering breast cancer risk in human. However, few studies have been conducted to illustrate the possible chemopreventive effects of Korean traditional food materials. This study was performed to determine the cytotoxic and apoptotic effects of yellow soybeans, black soybeans and brown rice extracts on hormone-dependent and hormone-independent human breast cancer cells. Methanol-or acetone-soluble fractions of soybeans or brown rice were incubated with hormone-dependent cells (MCF-7) or hormone-independent cells (MDA-MB-231). Cell cytotoxicity was measured by MTT assay at 24, 48 and 72 hrs of incubation. Apoptotic effects of these extracts toward breast cancer cells were also determined at 48 hrs of incubation by measuring DNA fragmentation. Results indicated that the acetone-soluble fraction of brown rice exerted strongest cytotoxic effect on MCF-7 ceIls, although other fractions also reduced the number of viable MCF-7 cells after 48 hrs of incubation. Both acetone and methanol soluble fractions of all samples exerted a significant cytotoxicity towards MDA-MB-231 cells after 24 hrs of incubation, and acetone and methanol soluble fractions of brown rice were especially effective in these cells. At 48 hrs of incubation, methanol fractions of all three samples induced apopotosis of MDA-MB-231 cells. These results indicate methaol or acetone soluble fractions of yellow soybeans, black soybeans and brown rice induce cytotoxicity in both hormone-dependent and hormone-independent breast cancer cells. Therefore, possible mechanisms of cell cytotoxicity do not necessarily include antiestrogenic effects of soybean or brown rice extract. A possible anticarcinogenic effect of brown rice methanol-soluble fraction may mediated through their apoptotic effect. Further studies are requried to elucidate responsible compounds and mechanisms involved in observed anticarcinogenesis.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.32 no.4
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• pp.409-416
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• 1987

The object of this study was to investigate the differences in dry matter production, physiological characteristic and grain yield of soybean (Glycine max (L.) Merr.) as affected by planting dates in southern region of Korea. This experiment was conducted using four planting dates and four varieties over 3 years (1982-1984). The different responses of soybean varieties to different temperature and daylength, days from planting to flowering were ranged from 43 to 55 days when planted on May 10 long daylength and low temperature condition. Flowering duration was ranged from 12 to 27 days and 9 to 13 days for the plantings made on May 10 and July 9, respectively. Therefore, it was the shortest under the short daylength and high tempera-ture condition. It also appeared that the later planting caused the lower dry matter weight and leaf area index which resulted in lower grain yield. But grain yield was not directly correlated with the unbalance of Xink/Source Ratio and the lodging from excessive vegatative growth. The grain yield was the highest in May 30 and the lowest in July 9 planting: it was ranged from 2.26 to 2.55 t/ha in the former and 0.85 to 1.20 t/ha in the latter, respectively. The increase in yield was depended on the increase in the number of pods per plant, the number of seeds per pod and the seed weight. The distribution ratio of dry matter to leaves was the hightest at the sixth trifoliolate leaf stage and gradually declined thereafter in all planting dates, and it also appeared that the later planting, the lower the ratio of dry matter to leaves.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.33 no.s01
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• pp.86-97
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• 1988

Sesame(Sesamum indicum L.) is probably the most ancient oilseed crop known in the world. The seed of sesame is used in a variety of ways as food. The whole seed may be eaten raw, either roasted or parched, or fed to birds and stock. Sesame oil is used as a salad or cooking oil, in shortening, margarine and in the manufacture of soap. Minor uses are as a fixative in the perfume industry and formerly as a carrier for fatsoluble substances in pharmaceuticals such as penicillin. One of the minor constituents of sesame oil, sesamin, is used for its synergistic effect in pyrethrin insecticides, in addition of a small quantity of this substance markedly increases the effectiveness of fly sprays. The meal remaining after oil extraction can be used as and animal feed-stuff or as manure. In general sesame meal is considered to be equal to cottonseed or soybean meal as a protein supplement for livestock and poultry. It is especially high in certain amino-acids such as methionine, which is low in soybean meal, and thus can be combined with it or similar meal to form a more balanced ration. An attempt to summarize the literature review on quality improvement of sesame was made to discuss the accomplishments of the past and perspectives in the future. The reviews on quality improvement of sesame were mainly discussed in connection with the cultural practices and genetic informations in current status. The emphasis focussed on environmental variation of quality in cultural practices, such as harvest time, variety by location, climatic condition, fertilizer application, and growth regulator treatment. On the genetic variation of quality, it was discussed on variety background, mutation breeding, correlations, and inheritance of quality related characteristics. It also was discussed on relationship between quality and plant traits, storage condition or period, and seed coat color. Moreover, current research status were reviewed on some minor elements such as sesamin, oxalic acid, and trypsin inhibitor. As a results of the review, the lack of an effort to quality improvement in each utilization area was indicated as a problem area. More active efforts for the improvement of quality were also insufficient to incorporate the available genes for quality in breeding method or collection and analysis of breeding materials. Therefore, researches in the future would be recommended to emphasize on these problem areas.

• Korean Journal of Soil Science and Fertilizer
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• v.17 no.4
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• pp.415-427
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• 1984

This experiment was carried out to investigate the distribution of associative nitrogen fixers, Azospirillum spp. and their nitrogenase activities measured by ARA in the rhizosphers of rice, soybean, and weed grown in the rice paddy field at ear formation stage of rice. Nitrogenase activities produced by Azospirillum spp. enriched from histophere ranged from 16 to 53 n mole/tube/hr.. High nitrogen fixing activities more than 30 n mole/tube/hr. were observed in the histophere of the Echinochloa crus-galli L., Finbristylis miliacea L., and Monochoria vaginalis var.. Nitrogen fixing activities of Azospirillum spp. obtained from single colonies which originated from the rhizoplane of rice (pot-kwang var.), Finbris tylis miliacea L., Monochoria vaginaliz var., Glycine max L. were higher over 100 n mole/tube/hr. than those histophere. Genus of Azopsirillum isoltated from roots of the Graminease (Oriza sativa L., $C_3$-plant, Echinochloa crus=galli L., $C_4$-plant, Cyperus difforuis L.. $C_4$-plant), and Aeschynomene indica L. (Leguminosae, $C_3$-plant) was identified as A. brasilense. However, both strains, A. lipoferum and A. brasilense ($nir^-$ or $nir^+$ strain) were isolated from other plant roots, Both $nir^-$ and $nir^+$ strains of A. brasilense were associated with the same host plant.

• Journal of Plant Biotechnology
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• v.36 no.1
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• pp.87-95
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• 2009

The recombinant DNAs, pGBF, pGTF, and pZ4F, using soybean ferritin gene have constructed with the promoters derived from seed proteins, glutelin, globulin, and zein. The recombinant ferritin genes were transformed into rice plant by Agrobacterium-mediated transformation. Iron contents and agronomic traits have been evaluated in the transgenic progenies. The embryogenic calli survived from second selection medium were regenerated at the rates of 19.2% with pGBF, 15.0% with pGTF, and 18.4% with pZ4F in Donganbyeo and 6.7% with pGBF, 11.7% with pGTF, and 3.4% with pZ4F in Hwashinbyeo. The introduction of ferritin gene in putative transgenic rice plants was confirmed by PCR and Southern blot analysis and also the expression of ferritin gene was identified by Northern blot and Western blot analysis. The iron accumulation in transgenic rice grains of the transgenic rice plant, T1-2, with zein promoter and ferritin gene contained 171.4 ppm showing 6.4 times higher than 26.7 ppm of Hwashinbyeo seed as wild type rice, but the transgenic plants with globulin and glutelin showed a bit higher iron contents with a range from 2.1 to 3.0 times compare to wild type grain. The growth responses of transgenic plants showed the large variances in plant height and number of tillers. However, there were some transgenic plants having similar phenotype to wild type plants. In the T1 generation of transgenic plants, plant height, culm length, panicle length, and number of tillers were similar to those of wild type plants, but ripened grain ratio ranged from 53.3% to 82.2% with relatively high variation. The transgenic rice plants would be useful for developing rice varieties with high iron content in rice grains.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.53 no.spc
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• pp.89-95
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• 2008

This study was carried out to examine growth characteristics and seed qualities of 1,296 Korean soybean landraces. The range of days to flowering, and days to maturity was 38 to 83 days and 47 to 102 days, respectively. The range of growth days were 105 to 160 days, and 38% was belonged to maturity group III. The 100 seed weight was 19.5g, showing the range of 2.4 g to 40.4 g, and 19.5 g in mean. 35.3% was in the range from 13.1 g to 20.0 g, and 29.4% in the range from 20.1 g to 25.0 g. Mean contents of crude protein was 41.8%, showing the range from 32.7% to 49.2%. Mean contents of crude oil was 18.0%, showing the range from 11.8% to 22.2%. The composition of unsaturated fatty acids were from 81.8% to 94.2%, and 85.4% in mean. Sucrose contents were in the range from 1.24% to 7.91% with the mean 5.21%, and oligo-saccharide contents from 2.45% to 11.13% with the mean 8.01%. Total isoflavone contents were in the range from $278.4\;{\mu}g/g$ to $2,736.9\;{\mu}g/g$ with the mean $1,066.8\;{\mu}g/g$. Among isoflavone contents, daidzein, glycitein, and genistein contents were in the range from $48.8\;{\mu}g/g$ to $1,709.6\;{\mu}g/g$ with the mean $483.2\;{\mu}g/g$, from $0.98\;{\mu}g/g$ to $892.3\;{\mu}g/g$ with the mean $111.6\;{\mu}g/g$, and from $79.8\;{\mu}g/g$ to $1242.3\;{\mu}g/g$ with the mean $472.0\;{\mu}g/g$, respectively.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.7 no.1
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• pp.139-144
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• 1969

1. In order to obtain useful mutants such as early maturity, resistance to lodging, high protein and oil content, and capability of high yield, dormant seeds of two soybean varieties, Jang Dan Baik Mok and Clark, were treated with ${\gamma}$-ray, Ethyl Methane Sulfonate(EMS), Ethylene Imine(EI)and combinations of ${\gamma}$-ray and EMS or EI. 2. The germination rate and survival rate in a variety Jang Dan Baik Mok were significantly decreased with ${\gamma}$-ray treatment while it was not the same in the Clark variety. A significant decrease for seedling height measured at 14 and 21 days after sowing was found with the increase of ${\gamma}$-ray dose in both varieties. 3. Germination rates in both varieties were significantly decreased as EI concentration increases, particularly severe damage in germination was observed at 0.008 Mo. concentration. Germination rate damages were found with EMS concentration increases in the variety Jang Dan Baik Mok while no regular responses in seedling height were observed in the variety Clark. 4. Germination rate was significantly lowered with the combined treatment of EMS and ${\gamma}$-ray 24KR than that of EMS alone. In the treatments of ${\gamma}$-ray with three levels of EI concentration, the combined treatments except 24KR+EI 0.002 Mol. resulted in better germination than of EI alone. In both varieties, significant reduction in seedling height was observed in the combined treatments of ${\gamma}$-ray with various concentrations of EMS, whereas stimulation effect on seedling height was found with treatment of ${\gamma}$-ray EI +0.08Mol. 5. Germination rate, survival rate, and plant height as criteria of radio sensitivity, variety Jang Dan Baik Mok is moze sensitive to ${\gamma}$-ray, EMS, and EI than the variety Clark, and the varietal difference in responsibility to mutagen may be due to the genetic-constitution of the varieties.

• Korean Journal of Environmental Agriculture
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• v.19 no.4
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• pp.358-363
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• 2000

This study was carried out to obtain the basic data for selecting the applicable crops in reclaimed land during desalinization period. A pot experiment was conducted with 5 different electrical conductivities of the saturated extracts $(ECe\;1,\;3,\;9,\;14,\;and\;16\;dS{\cdot}m^{-1})$ of soils taken from the Dae-Ho reclaimed tidal lands. Eight crops (Chinese cabbage, radish, tomato, red pepper, buckwheat, soybean, sesame, and green perilla) were grown for 37days. Plant height and number of leaves were surveyed on 2 and 4 weeks after seeding, and on harvest time (5 weeks). After harvest, dry weights of harvested crops were measured and soil chemical properties were analyzed. Emergence rates of crops were comparatively high except sesame. For sesame, there was no emergence at ECe over $3\;dS{\cdot}m^{-1}$. Growth and dry weight decreased significantly as increasing ECe. The ECe which decreased 50% of dry weight index were $14.2\;dS{\cdot}m^{-1}$ for radish, $11.4\;dS{\cdot}m^{-1}$ for Chinese cabbage, $10.2\;dS{\cdot}m^{-1}$ for red pepper, $8.9\;dS{\cdot}m^{-1}$ for buckwheat and green perilla, $8.6\;dS{\cdot}m^{-1}$ for soybean, and $8.9\;dS{\cdot}m^{-1}$ for tomato. At higher ECe that start the growth inhibition, increasing $1\;dS{\cdot}m^{-1}$ in ECe, 7.7, 6.5, 5.9, 5.6, 5.2, and 4.9% of dry weight decreased for buckwheat, green perilla, Chinese cabbage, radish, soybean, and tomato (red pepper), respectively. The critical value of ECe for crop survival except sesame was $15.4\;{\sim}\;23.1\;dS{\cdot}m^{-1}$.