• The Plant Pathology Journal
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• v.21 no.1
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• pp.47-54
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• 2005

Identification of host genes involved in disease progresses and/or defense responses is one of the most critical steps leading to the elucidation of disease resistance mechanisms in plants. Soybean mosaic virus (SMV) is one of the most prevalent pathogen of soybean (Glycine max). Although the soybeans are placed one of many important crops, relatively little is known about defense mechanism. In order to obtain host genes involved in SMV disease progress and host defense especially for virus resistance, two different cloning strategies (DD RT-PCR and Subtractive hybridization) were employed to identify pathogenesis- and defenserelated genes (PRs and DRs) from susceptible (Geumjeong 1) and resistant (Geumjeong 2) cultivars against SMV strain G7H. Using these approaches, we obtained 570 genes that expressed differentially during SMV infection processes. Based upon sequence analyses, differentially expressed host genes were classified into five groups, i.e. metabolism, genetic information processing, environmental information processing, cellular processes and unclassified group. A total of 11 differentially expressed genes including protein kinase, transcription factor, other potential signaling components and resistant-like gene involved in host defense response were selected to further characterize and determine expression profiles of each selected gene. Functional characterization of these genes will likely facilitate the elucidation of defense signal transduction and biological function in SMV-infected soybean plants.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.67 no.4
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• pp.253-264
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• 2022

In this study, we investigated the occurrence of viral diseases in the early growth stage of soybean to establish management practices. We collected 83 soybean samples showing abnormal symptoms, approximately 3-4 weeks after seeding in the breeding field of the National Institute of Crop Science. Viruses were detected in the collected samples using reverse transcription polymerase chain reaction (RT-PCR) and metatranscriptome analysis of all those samples. The incidence of viral diseases in the field was less than 1% overall and up to 50% in certain cultivars and lines. RT-PCR and metatranscriptome analysis detected Soybean yellow mottle mosaic virus (SYMMV), Soybean mosaic virus (SMV), Soybean yellow common mosaic virus, Peanut stunt virus, and soybean geminivirus A (SGVA). Among these detected viruses, SYMMV and SMV were identified as major viruses causing infection in the early growth stage of soybean, with detection rates of 53.7% and 42.6%, respectively. Soybeans infected with SYMMV showed typical mosaic symptoms, whereas those infected with SMV showed a variety of symptoms such as mosaic, mottle, stunt, and chlorotic spots. Transmission characteristics of these viruses are variable, such that SMV is primarily transmitted by seeds, whereas SYMMV could be transmitted by insects, soil, and seeds. In this study, SGVA was detected in the early growth stage of soybean, and research on the current status and its effects on soybean after the early growth stage should be conducted.

• Proceedings of the Korean Society of Plant Pathology Conference
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• 2003.10a
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• pp.76.2-77
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• 2003

The complete nucleotide sequences of genomic RNA of an isolate of soybean mosaic virus (SMV-CN18), which has ability to overcome Rsv resistance of soybean, have been determined. A large open reading frame encodes a polyprotein of 3068 amino acids with a predicted Mr of 350 kDa. Based on comparison with the proposed cleavage site of other potyviral polyproteins, nine mature proteins are predicted as a following order, P1, HC-Pro, P3, CI, 6K, VPg, NIa, NIb and coat protein (CP). The mature proteins of the strain share various amino acid identity with known SMV-G2, -G7 and -N strain, with the greatest variability occurring in the P1 (91 ％, 88 ％, 96％)and the lowest variability in the CP (100 ％, 99 ％, 100 ％). In addition, 5' untranslated region determined by 5' RACE is much more various than any coding regions. Difference in amino acid sequences throughout the genome is discussed in relation to resistance and susceptibility of soybean cultivars to SMV-CNl8.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.29 no.3
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• pp.280-284
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• 1984

Association of SMV infection in soybean plants with seedcoat mottling and with seed transmission of SMV was examined. Seedcoat mottling was increased positively in proportion to the SMV severity in variety Clark. No correlation was found between the incidence of mottled seed and the seed's position on the plant. Susceptible variety with virus symptoms gave rise to mottled seeds but only a small percentage of these mottled seeds could transmit the virus. SMV could be transmitted even by nonmottled seeds harvested from SMV infected plants in similar rate with mottled seed. It seemed that the amount of mottled seed could be used as an indicator of the amounts of SMV infection in a seed production field.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.45 no.6
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• pp.353-355
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• 2000

The seasonal occurrence of aphids was investigated in the soybean Held to increase the control efficiency of aphid vectors of Soybean Mosaic Virus. The patterns of aphids occurrence were different according to planting time. There were two peaks in late June and mid-August in aphid population in optimum seeding (May 20), whereas the peak was around mid-June in early seeding(Apr, 20). Acyrthosiphon solani was dominant Species in early seeding, while Aphis glycines was dominant in optimum seeding. In early seeding, SMV incidence increased rapidly between 20 June and 30 June, suggesting that virus spread was strongly correlated with increased colonization of aphids. Imidacloprid WP, benfuracarb EC and acephate WP showed a good effect for the control of aphids without phytotoxicity. In the plot with infurrow treatment of imidachloprid G until 52 days after shooting, aphids were controled effectively. Acephate WP was applied as a foliar spray at V4, V6, or V4/V6 stage and all the treatments were effective on reducing: SMV incidence.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.38 no.4
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• pp.336-342
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• 1993

To obtain the basic information necessary for the development of soybean varieties well adaptable to upland-paddy field rotational croppings, the difference of growth characteristics between upland and paddy-field including yield potentials of current recommending soybean varieties were evaluated. The growth characteristics, both above and under-ground, which were measured at flowering stage were generally greater in paddy-field but the number of root nodules was much greater in upland, thus the artificial inoculation was practically recommended for soybean growing in paddy-fields. Mean seed yield was generally higher in paddy-fields than in upland. All soybean varieties showed higher seed yield in the early planting date, April 20, were somewhat susceptible to soybean mosaic virus(SMV), thus they could be escaped from the disasterous endemic necrotic soybean mosaic virus(SMV-N). Soybean varieties showed over 4.0 tons/ha seed yield in the paddy-field were Williams 79, Union, SS77053, and Namhaekong. At the same time, Jangyeobkong and Danyeobkong were the most stable soybean varieties among the tested soybean varieties with less than 10% of coefficient variation values in all planting dates in paddy fields. Compared with Hwangkeumkong which is most widely being cultivated on farmer's fields, soybean varieties showed high yields in paddy-field were higher in plant height, less in the number of branches, and more in the number of nodes on main stem. At the same time, they had medium seed size which would bring the good germination and stands. Disease resistance especially for necrotic soybean mosaic virus was also one of the most decisive factors in seed yields for the early planted soybeans.

• Korean journal of applied entomology
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• v.23 no.4 s.61
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• pp.197-202
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• 1984

Two soybean cultivars Hanan and Kwanggyo were used to monitor SMV spread in the field. The Haman producing mosaic symptoms by infection with SMV strain G7 was planted in $2.2m^2$ at the coater of $320.7m^2$ field and Kwanggyo producing necrosis by infection with SMV-G7 was planted around the Haman one day after inoculation of Heman with SMV-G7. The most severe incidence of the necrotic disease in soybean cultivar Kwanggyo occurred on 43 days after planting(July 13) whereas aphid population reached peak around 22 days after planting(June 22). Total incidence of the necrotic disease was $20.4\%$, in the whole field and $56\%$, in eight $mall plots around the spreader of SMV. The spread of S MV outward from the spreader source was greater downwind than it was upwind. Spread also showed a signigicant gradieut pattern leeward from the infection focus.

• Korean journal of applied entomology
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• v.15 no.2 s.27
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• pp.61-68
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• 1976

Leading soybean cultivars such as Kwanggyo, Yugu No.3, Dongbugtae, Gangrim, and Eundaedu were heavily diseased by a virus in Korea. The disease was most severe in the northern provinces where soybean mosaic virus also occurrs, but the disease has also been observed in other provinces where soybean diseases are less prevalent. The disease symptoms were similar to bud blight caused by tobacco ringspot virus; but this was not confirmed in inoculation tests on indicator plants and serological experiments. There were some differences in varietal susceptibility to the disease, with symptom variation depending on the soybean cultivar and source of inoculm. Disease symptoms on infected soybean plants were mottling and necrosis. The present results, therefore, indicate some strains of SMV or a mixture of legume viruses may or may not be responsible for the disease.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.44 no.3
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• pp.253-255
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• 1999

Reverse transcription and polymerase chain reaction (RT-PCR) assay was used to detect SMV strains. A pair of oligonucleotide primers were designed to include the cylindrical inclusion (CI) coding region between 4,176 to 5,560 nt. Amplification from the total RNA extracted from infected plants with SMV yielded a 1,385 bp DNA fragment. RT-PCR was shown to be $10^3$ times more sensitive than the ELISA assay and it could detect a virus in $10^{-6}$ dilution. Restriction enzyme analysis of RT- PCR products using EcoR I showed that SMV isolates were classified into six groups according to the patterns of restriction fragments.

• Korean journal of applied entomology
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• v.25 no.2 s.67
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• pp.113-120
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• 1986

Soybeans collected from different locations in Korea were planted for tests of seed transmission of soybean mosaic virus (SMV). The percentage of seed transmission ranged from 20.5 to 29.5% in 14 seedlots including soybean cultivar Namhe and 12 to 19% in other 44 seedlots. However, no seed transmission of SMV was observed in soybean cultivar Younkiyongho. SMV was detected from embryo and cotyledon of soybean seeds. The infection of SMV was highly detected from premature seeds than from fully mature seeds, and higher from seeds harvested from plants infected before June 20 than plants infected around July 20 and August 20. No significant relationship was observed between seed transmission of SMV and mottling of seeds. The incidence of soybean mosaic disease followed by one month after peak of aphid population. The number of aphids was less on leaves of soybeans with short and dense trichomes whereas it was higher on leaves of soybean with long and sparse trichomes. Generally, the number of aphids was decreased on leaves with long and dense trichomes as the growth progressed. Soybean cultivar Columbus and 14 cultivars were susceptible, Chief and 14 cultivars were moderate, but Jangbaek and 17 other cultivars were resistant to SMV when inoculated with one isolate of SMV.