KOREAN JOURNAL OF CROP SCIENCE (한국작물학회지)

The Korean Society of Crop Science (한국작물학회)
권호 표지
DOI QR코드

DOI QR Code

Occurrence of Viral Diseases in the Early Growth Stage of Soybean in Korea

우리나라 콩 생육초기 바이러스병 발생 양상

  • Sangmin Bak (School of Applied Biosciences, Kyungpook National University) ;
  • Mina Kwon (School of Applied Biosciences, Kyungpook National University) ;
  • Dong Hyun Kang (School of Applied Biosciences, Kyungpook National University) ;
  • Hong-Kyu Lee (School of Applied Biosciences, Kyungpook National University) ;
  • Young-Nam Yoon (Crop Production Technology Research Division, Department of Southern Area Crop Science, National Institute of Crop Science) ;
  • In-Yeol Baek (Upland Crop Breeding Division, Department of Southern Area Crop Science, National Institute of Crop Science) ;
  • Young Gyu Lee (Highland Agriculture Research Institute, National Institute of Crop Science) ;
  • Jae Sun Moon (Plant System Engineering Research Center, Korea Research Institute of Bioscience and Biotechnology) ;
  • Su-Heon Lee (School of Applied Biosciences, Kyungpook National University)
  • 박상민 (경북대학교 응용생명과학부 ) ;
  • 권민아 (경북대학교 응용생명과학부 ) ;
  • 강동현 (경북대학교 응용생명과학부 ) ;
  • 이홍규 (경북대학교 응용생명과학부 ) ;
  • 윤영남 (국립식량과학원 남부작물부 생산기술개발과 ) ;
  • 백인열 (국립식량과학원 남부작물부 밭작물개발과 ) ;
  • 이영규 (국립식량과학원 고령지농업연구소 ) ;
  • 문제선 (한국생명공학연구원 식물시스템공학연구센터 ) ;
  • 이수헌 (경북대학교 응용생명과학부 )
  • Received : 2022.10.12
  • Accepted : 2022.11.10
  • Published : 2022.12.01
Download PDF
⟨ Previous Next ⟩

Abstract

In this study, we investigated the occurrence of viral diseases in the early growth stage of soybean to establish management practices. We collected 83 soybean samples showing abnormal symptoms, approximately 3-4 weeks after seeding in the breeding field of the National Institute of Crop Science. Viruses were detected in the collected samples using reverse transcription polymerase chain reaction (RT-PCR) and metatranscriptome analysis of all those samples. The incidence of viral diseases in the field was less than 1% overall and up to 50% in certain cultivars and lines. RT-PCR and metatranscriptome analysis detected Soybean yellow mottle mosaic virus (SYMMV), Soybean mosaic virus (SMV), Soybean yellow common mosaic virus, Peanut stunt virus, and soybean geminivirus A (SGVA). Among these detected viruses, SYMMV and SMV were identified as major viruses causing infection in the early growth stage of soybean, with detection rates of 53.7% and 42.6%, respectively. Soybeans infected with SYMMV showed typical mosaic symptoms, whereas those infected with SMV showed a variety of symptoms such as mosaic, mottle, stunt, and chlorotic spots. Transmission characteristics of these viruses are variable, such that SMV is primarily transmitted by seeds, whereas SYMMV could be transmitted by insects, soil, and seeds. In this study, SGVA was detected in the early growth stage of soybean, and research on the current status and its effects on soybean after the early growth stage should be conducted.

본 연구는 콩 바이러스병 관리대책 수립을 위하여 생육 초기에 발생하는 바이러스병의 감염실태를 조사하였다. 국립식량과학원 육종포장에서 파종 후 약 3-4주 된 콩 이상증상 시료 83점을 채집하였다. 채집한 시료의 감염 바이러스 진단은 12종 바이러스의 RT-PCR 진단과 전체 시료에 대한 메타전사체 분석을 통하여 수행하였다. 1. 포장에서 바이러스병 발병율은 전체적으로 1% 미만으로 조사되었으며 일부 품종 및 라인에서는 최대 50%까지 나타내었다. 2. RT-PCR 진단과 메타전사체 분석 결과 SYMMV, SMV, SYCMV, PSV, SGVA가 검출되었다. 3. 검출된 바이러스 중에서 SYMMV와 SMV는 각각 검출율이 53.7%, 42.6%로 나타내어 콩 생육초기에 발생하는 주요 바이러스로 확인되었다. 4. SYMMV에 감염된 콩은 전형적인 모자이크 증상을 나타내었으며, SMV에 감염된 콩은 모자이크, 얼룩, 위축, 퇴록반점과 같이 다양한 증상을 나타내었다. 5. 바이러스 전염 특성을 고려할 때 SMV는 주로 종자전염을 통하여 발생한 것으로 판단되며, SYMMV는 종자전염과 함께 충매전염 및 토양전염의 가능성이 복합적으로 고려되어야 할 것으로 판단된다. 6. SGVA는 본 연구를 통하여 콩 생육초기 포장에서 검출되었으며, 생육초기 이후 발생실태 및 콩에 미치는 영향에 관한 연구가 신속히 수행되어야 할 것이다.

Keywords

Acknowledgement

본 논문은 농촌진흥청 연구사업(세부과제번호: PJ01486803)의 지원에 의해 이루어진 것임.

References

  1. Cho, E. K. 1981. Strains of soybean mosaic virus with emphasis on the mechanism of aphid transmissibility. Korean J. Pl. Prot. 20(3) : 177-180.
  2. Cho, E. K. 1995. Identification of Soybean Mosaic Virus Strains and a Consideration on Genetics of Soybean for Resistance to SMV Strains. Plant Dis. and Agriculture. 1(2) : 22-25.
  3. Cho, E. K. and B. J. Chung. 1976. Studies on Identification and Classification of Soybean Virus Diseases in Korea. Korean J. Plant Prot. 15(2) : 61-68.
  4. Cho, E. K. and R. M. Goodman. 1982. Field Spread of Soybean Mosaic Virus Strains. Korean J. Plant Prot. 21(2) : 53-60.
  5. Cho, E. K. and S. H. Lee. 2003. Cowpea mosaic virus from Vegetable Soybeans in Korea. Plant Pathol. J. 19(3) : 166-170. https://doi.org/10.5423/PPJ.2003.19.3.166
  6. Cho, E. K., S. H. Choi, and C. Y. Hwang. 1984. Epidemiology of Soybean Mosaic Virus Diseases. Korean J. Plant Prot. 23(4) : 197-202.
  7. Cho, S., J. Kim, M. Li, E. Seo, S. Lim, S. M. Hong, J. S. Moon, J. Hammond, and H. S. Lim. 2013. Occurrence of Three Major Soybean Viruses, Soybean mosaic virus, Soybean yellow mottle mosaic virus and Soybean yellow common mosaic virus Revealed by a Nationwide Survey of Subsistence Farming Soybean Fields. Res. Plant Dis. 19(4) : 319-325. https://doi.org/10.5423/RPD.2013.19.4.319
  8. Fiallo-Olive, E., J. M. Lett, D. P. Martin, P. Roumagnac, A. Varsani, F. M. Zerbini, and J. Navas-Castillo. 2021. ICTV Virus Taxonomy Profile: Geminiviridae 2021. J. Gen. Virol. 102(12) : 1-2.
  9. Kil, E. J., J. Park, H. S. Choi, C. S. Kim, and S. Lee. 2017. Seed Transmission of Tomato yellow leaf curl virus in White Soybean (Glycine max). Plant Pathol. J. 33(4) : 424-428. https://doi.org/10.5423/PPJ.NT.02.2017.0043
  10. Kim, J. S., S. H. Lee, H. S. Choi, M. K. Kim, H. R. Kwak, J. S. Kim, M. Nam, J. D. Cho, I. S. Cho, and G. S. Choi. 2012. 2007-2011 Characteristics of Plant Virus Infections on Crop Samples Submitted from Agricultural Places. Res. Plant Dis. 18(4) : 277-289. https://doi.org/10.5423/RPD.2012.18.4.277
  11. Kim, S. M., J. B. Kim, Y. H. Lee, S. H. Choi, H. S. Choi, J. W. Park, J. S. Lee, G. S. Lee, J. K. Moon, J. S. Moon, K. W. Lee, and S. H. Lee. 2006. First Report of Soybean Dwarf Virus on Soybean (Glycine max) in Korea. Res. Plant Dis. 12(3) : 213-220. https://doi.org/10.5423/RPD.2006.12.3.213
  12. Kim, Y. H., O. S. Kim, B. C. Lee, J. K. Moon, S. C. Lee, and J. Y. Lee. 2003. G7H, a new Soybean mosaic virus strain: Its virulence and nucleotide sequence of CI gene. Plant Dis. 87 : 1372-1375. https://doi.org/10.1094/PDIS.2003.87.11.1372
  13. Lee, H., W. Song, H. R. Kwak, J. D. Kim, J. Park, C. K. Auh, D. H. Kim, K. Y. Lee, S. Lee, and H. S. Choi. 2010. Phylogenetic analysis and inflow route of Tomato yellow leaf curl virus (TYLCV) and Bemisia tabaci in Korea. Mol. Cells. 30 : 467-476. https://doi.org/10.1007/s10059-010-0143-7
  14. Lee, J. D., J. K. Yu, Y. H. Hwang, S. Blake, Y. S. So, G. J. Lee, H. T. Nguyen and J. Grover Shannon. 2008. Genetic Diversity of Wild Soybean (Glycine soja Sieb. and Zucc.) Accessions from South Korea and Other Countries. Crop Science 48 : 606-616. https://doi.org/10.2135/cropsci2007.05.0257
  15. Lee, S. H., Y. M. Choi, J. S. Kim, and B. J. Chung. 1985. Identification of Alfalfa Mosaic Virus from Soybean. Korean J. Plant Pathol. 1(1) : 33-37.
  16. Lee, S. H., M. H. Lee, and H. Tochihara. 1980. An infectious virus isolated from soybeans. Korean J. Plant Prot. 9(3) : 175-179.
  17. Lee, S. H. and C. S. Kim. 2013. Natural Hosts and Disease Cycle of Soybean yellow mottle mosaic virus. Res. Plant Dis. 19(4) : 281-287. https://doi.org/10.5423/RPD.2013.19.4.281
  18. Lee, S. H., J. B. Lee, S. M. Kim, H. S. Choi, J. W. Park, J. S. Lee, K. W. Lee, and J. S. Moon. 2004. The Incidence and Distribution of Viral Diseases in Pepper by Cultivation Types. Res. Plant Dis. 10(4) : 231-240. https://doi.org/10.5423/RPD.2004.10.4.231
  19. Lee, S. H., M. Nam, H. S. Choi, J. S. Lee, J. S. Kim, I. Y. Baek, and J. K. Moon. 2011. Specific primers for multiple detection of the viruses in soybean, and uses thereof. R.O.K Patent 10-1250388.
  20. Lee, T. S. 2009. The Life Cycle of SYMMV Infected in Soybean. Master's thesis, Jeonbuk National University.
  21. Lee, Y. H. and T. S. Park. 2006. Origin of Legumes Cultivation in Korea Peninsula by Viewpoint of Excavated Grain Remains and Genetic Diversity of Legumes. Korean Agricultural History Association 5(1) : 1-31.
  22. Lee, Y. H., S. T. Lim, Y. N. Yoon, M. G. Jeon, H. T. Yun, J. M. Ko, S. H. Lee, K. W. Lee, and I. Y. Baek. 2012. Incidence of Soybean Viral Diseases in Korea. Korea Soybean Digest. 29(1) : 7-15.
  23. Lee, Y. H., Y. N. Yoon, H. T. Yun, I. Y. Baek, S. Lim, J. S. Moon, and S. H. Lee. 2015. First Report of Bean common mosaic virus Infecting Soybean in South Korea. Plant Dis. 99(8) : 1189.
  24. Lim, S., Y. H. Lee, D. Igori, F. Zhao, R. H. Yoo, S. H. Lee, I.Y. Baek, and J. S. Moon. 2014. First Report of Peanut mottle virus Infecting Soybean in South Korea. Plant Dis. 98(9) : 1285.
  25. Lim, W. S., Y. H. Kim, and K. H. Kim. 2003. Complete Genome Sequences of the Genomic RNA of Soybean mosaic virus Strains G7H and G5. Plant Pathol. J. 19(3) : 171-176. https://doi.org/10.5423/PPJ.2003.19.3.171
  26. MAFRARK. 2021. Statistical Yearbook of Agriculture, Food and Rural Affairs, Sejong, Republic of Korea.
  27. Nam, M., J. S. Kim, S. J. Park, C. Y. Park, J. S. Lee, H. S. Choi, J. S. Kim, H. H. Kim, S. Lim, J. S. Moon, and S. H. Lee. 2012a. Biological and molecular characterization of Soybean yellow common mosaic virus, a new species in the genus Sobemovirus. Virus Res. 163(1) : 363-367. https://doi.org/10.1016/j.virusres.2011.08.005
  28. Nam, M., S. M. Kim, L. L. Domier, S. Koh, J. K. Moon, H. S. Choi, H. G. Kim, J. S. Moon, and S. H. Lee. 2009. Nucleotide sequence and genomic organization of a newly identified member of the genus Carmovirus, soybean yellow mottle mosaic virus, from soybean. Arch Virol. 154(10) : 1679-1684. https://doi.org/10.1007/s00705-009-0480-z
  29. Nam, M., S. J. Park, Y. J. Kim, J. S. Kim, C. Y. Park, J. S. Lee, H. S. Choi, J. S. Kim, and S. H. Lee. 2012b. First Report of Peanut stunt virus on Glycine max in Korea. Plant Pathol. J. 28(3) : 330.
  30. Park, C. Y., H. G. Min, H. K. Lee, R. Maharjan, Y. Yoon, and S. H. Lee. 2018. Development of a Multiplex Reverse Transcription-Polymerase Chain Reaction Assay for the Simultaneous Detection of Three Viruses in Leguminous Plants. Res. Plant Dis. 24(4) : 348-352. https://doi.org/10.5423/RPD.2018.24.4.348
  31. Sandra, N., A. Tripathi, H. K. Dikshit, B. Mandal, and R. K. Jain. 2020. Seed transmission of a distinct soybean yellow mottle mosaic virus strain identified from India in natural and experimental hosts. Virus Res. 280 : 197903.
  32. Seo, J. K., J. Y. Park, H. R. Kwak, M. K. Kim, M. Nam, S. H. Lee, J. S. Kim, and H. S. Choi. 2014. Severe Outbreak of the Emerging Soybean Viruses in Korea: An Increasing Threat. Plant Health Prog. 15(3) : 122.
  33. Shin, J. C., M. K. Kim, H. R. Kwak, H. S. Choi, J. S. Kim, C. Y. Park, and B. J. Cha. 2014. First Report of Clover yellow vein virus on Glycine max in Korea. Plant Dis. 98(9) : 1283.
  34. Yoon, Y. N., Y. Jo, W. K. Cho, H. Choi, Y. Jang, Y. H. Lee, J. Y. Bae, and B. C. Lee. 2017. First Report of Tomato spotted wilt virus Infecting Soybean in Korea. Plant Dis. 102(2) : 461.