• Title/Summary/Keyword: Soy bean extract

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Comparative Estrogenic Effects of Yak-Kong and Soy Bean on the Proliferation of Human Osteoblastic Cell Line, MG-63 (MG 63 조골세포에서 약콩과 대두의 천연 에스트로겐 효과 비교)

  • 조윤희;박수진;신호정;장기효;강순아;조여원
    • Journal of Nutrition and Health
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    • v.34 no.8
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    • pp.905-911
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    • 2001
  • Phytoestrogens, especially soy-derived isoflavones, are receiving great scrutiny as a food supplement for preventing hormone dependent diseases such as cardiovascular diseases, cancer, and osteoporosis. These beneficial effects of phytoestrogens are caused by functioning as partial agonists or antagonists of estrogens. In contrast to the common usage of soy bean, Yak-kong(Rhynchosia Molubilis ; ) has been used as supplements of estrogen fir preventing postmenopausal osteoporosis in Oriental medicine. To investigate estrogenic effects of Yak-kong and soy bean on the proliferation of MG-63 osteoblastic cells, each bean was extracted with 70% methanol and dried by freeze-drying. Yak-kong treatment of MG-63 cells resulted in an increase of cell proliferation to a maximum of 76% compared to 68% of soy bean treatment. Treatment of MG-63 cells with Yak-kong extract also resulted in an increase of transactivation of an ERE(estrogen response element)-luciferase reporter plasmid and IGF-I expression selectively. Despite increased effects of both bean treatments on the expression of estrogen receptor $\alpha$(ER$\alpha$) and $\beta$(ER$\beta$), soy bean treatment decreased transactivation of an ERE-luciferase reporter plasmid and did not further enhance IGF-I expression. Together, our data demonstrates that the greater estrogenic response of Yak-kong extract for MG-63 cell proliferation is mediated by ER derived transactivation of ERE and selective induction of IGF-I expression.

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The Study on the Detection of Aflatoxins in the Fermentation Products and Cereals (TLC법에 의한 장류 및 공류중의 Aflatoxin검출에 관한 연구)

  • 한양일;김광호;오영복
    • Journal of Environmental Health Sciences
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    • v.5 no.1
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    • pp.46-50
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    • 1978
  • Aflatoxin, a mixture of the at least four toxic and carcinogenic metabolites, is known to be produced by only a few fungi. The toxins were designated aflatoxins because they were produced by the mold Aspergillus flavus(A. flavus). However, at least four other toxins and other species of the genus A. niger, A. parasiticus A. ruber and wentii have been reported to produce aflatoxins. And also the identical compounds may also be produced by molds, the Pencillium. At least four different species of Penicilliurn have been reported to produce aflatoxins (P. citrinurn, P. frequentans, P. puberulurn. and P. variable). So it is now known that the problem of Aflatoxin is not restricted to the single species A. flavus, even though that is a very common mold. Also additional aflatoxins have been discorvered. For sereral years, only four aflatoxins were known: $B_1, B_2, G_1$ and $G_2$, so designated by reason of their fluorescence and chromatographic charateristics. It is now known that there are really two new toxic materials in the milk. During the past year(1966) they were christened aflatoxin $M_1$ and $M_2$, since they were first found in milk. The two other and most recently discorvered aflatoxins were isolated late in 1966 from cultures of A. flavus, and were designated aflatoxin $B_2a$ and aflatoxin $G_2a$. In order to obtain a breaf information about extent of contamination of foodstuffs by aflatoxin which is known to produce eight different mold, aflatoxin detection of cereals and fermented foods on sale, such as polished rice, barley, wheat, wheat flour, lentil, red bean, soy bean, noodle, kochuj ang and Dwenjang (fermented soy bean paste) and chong Kuk, were carried out. The results of this investigation were summarized as follows: The hexane:$CHCl_3$ extracts of polished rice, barley wheat, wheat flour, lentil, red bean, noodle and kochujang yielded fluorescent spots on thin layer plates. However their Rfvalues were different from those of authentic aflatoxins. The fluorescent substances of the extract from soy bean, Dwenjang and chong kuk showed very similar Rf values to those of the standard aflatoxins. By two dimensional thin layer chromatography and comparison of ultra violet absorption spectra, it was found that these fluorescent substances were not aflatoxins. To conclude, aflatoxins themselves were not detected directly in those samples tested.

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Effect of Fermented Soy Bean Extract Containing Herbal Medicines (Godjang) on Blood Glucose Levels and Histomorphology in Streptozotocin-induced Diabetic Rat (한약재 함유 대두발효 추출물이 streptozotocin으로 유발된 당뇨 흰쥐의 혈당 및 조직형태학적 변화에 미치는 영향)

  • Jo, Chang Suk;Kim, So Young;Choi, Moon-Yeol;Kim, Mi Hyung;Kim, Mi Ryeo;Seo, Bu-il
    • The Korea Journal of Herbology
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    • v.37 no.1
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    • pp.1-9
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    • 2022
  • Objective : This study was designed to investigate anti-diabetic effects of fermented soy bean extract with herbal medicines (Godjang) in diabetic rat models induced by streptozotocin (STZ) injection. Method : Changes in body weight, drinking water, and food intake were observed for 4 weeks before and after induction of diabetes mellitus in rats. The anti-diabetic capacity of Godjang was analyzed by fasting blood glucose (FBG) every week. Also, after 4 weeks of administration, the oral glucose tolerance test (OGTT) was performed, and then blood levels of insulin were checked. And serum levels of total cholesterol and triglycerides were determined. Histomorphological changes of liver, kidney and pancreatic tissues were also observed in STZ-induced diabetic rats and Godjang administered rats. Result : In Godjang administered group, body weight and water intake were more lower than that of STZ-induced diabetic rats. FBG was decreased in the Godjang administered group than STZ-induced diabetic group. According to OGTT, blood glucose levels at 30 minutes and 60 minutes significantly decreased in Godjang administered group than in STZ-induced diabetic control group. Administration of Godjang extract for 4W significantly decreased levels of serum glucose, total cholesterol (TC), triglycerides (TG) in diabetic rats. In histomorphological analysis of kidney, liver, Godjang administrated groups showed the inhibition of pathological damage. Conclusion : These results suggest that Godjang extract has an anti-diabetic action through decrease in serum glucose, TC, TG levels and recovery of the morphological changes in kidney and liver in STZ-induced diabetic rats.

Improving Effect for Acne with SeleMix AN Composed of Germinating Soy Bean and Magnolia Bark Extract (발아콩 및 목련박피 혼합추출물(SeleMix AN)에 의한 여드름 개선 효과)

  • Ryu, Jong-Seong;Kim, Jin-Hwang;Kwak, Taek-Jong;Kim, Ki-Sun;Kim, Jin-Jun;Lee, Cheon-Koo;Park, Kyung-Chan
    • Journal of the Society of Cosmetic Scientists of Korea
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    • v.33 no.1 s.60
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    • pp.29-32
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    • 2007
  • We investigated new ingredients with real efficacy in both in vitro and in vivo all together. Especially we focused on the real improving effort in the clinical experiments. Because most products containing effective materials evaluated in vitro failed to show a real improving effect in the human with acne. We evaluated the well-known ingredients in a small scale clinical experiment with half-finished goods containing each ingredient. Among these products, product formulating SeleMix AN composed with germinating soy bean and magnolias bark extract remarkably improved acne and acne scar. Moreover skin redness caused by severe acne was improved. There was statistical significance between placebo and sample. Two hundred volunteers participated in our pilot study with written informed consent. After then we performed in vitro efficacy test this ingredient. SeleMix AN inhibited the growth of propionibacterium acnes at a concentration of 0.0125% and suppressed histamine release by 16.9%. Moreover human fibroblast cell activity was increased by 57% compared to control. Lastly, we performed a clinical study. Consisting of groups of 23 volunteers. Although the period of the test was in summer accelerating sebum secretion and recurring a high tate of acne, inflammation lesions were especially improved after applying product containing SeleMix AN for 4 weeks.

Decrease efficiency of Offensive Odor from Pig Excreta by Yeast Strain, Pichia farinosa NASS-2 Isolated from Soy Bean Paste (된장에서 분리한 효모(Pichia farinosa NASS-2)의 돈분 악취감소효과)

  • Yoo, Jae Hong;Park, In Cheol;Kim, Wan Gyu
    • The Korean Journal of Mycology
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    • v.40 no.4
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    • pp.254-257
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    • 2012
  • The different microbial species were isolated from soy bean paste samples. A yeast strain NASS-2 was effective to decrease odor activity on pig excreata was identified as Pichia farinosa based on nucleotide sequences of 18S ribosomal DNA and Internal transcribed space (ITS). The extracellular fraction of P. farinosa NASS-2 was effective to decrease odor activity of pig excrements. Optimal medium component for decreasing order activity on odor material composed of soluble starch 2.0% (w/v) and yeast extract 0.8% (v/v). The decrease of odor material was maximum at $28^{\circ}C$ for 72 hours with pH 5.5. When the P. farinosa NASS-2 culture broth was treated to pig excrements, the removal efficiency was an average concentration with 1.38 ppm of ammonia gas.

Sensory Evaluation of Chungkukjangs with Herbal Extracts and Clinical Evaluation in Atopy Dermatitis Patients (천연물(알로에, 계피, 감초) 첨가 청국장의 관능평가와 아토피 환자에서의 임상적 효능 평가)

  • 윤성하;이상선;장정은;노건웅
    • Journal of Nutrition and Health
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    • v.37 no.8
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    • pp.669-674
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    • 2004
  • The purpose of this study was to develop the antiallergic and hypoallergic fennented soybean foods without side effect. We manufactured Chungkukjang with addition of herbal (aloe, cinamon, licorice root) extract. Sensory evaluation was performed to evaluate the acceptability by the consumer. Clinical evaluation was performed with 10 atopic dermatitis (AD) patients who showed positive reaction with specific IgE and skin prick test. Cross-over study between nonnal Chungkukjang and Chungkukjang with aloe extract was performed. In sensory evaluation, Chungkukjang with aloe extract obtained best score overall. In clinical evaluation, 7 out of 10 AD patients showed positive reaction to soy-bean and 4 out of 10 AD patients showed positive reaction to normal Chungkukjang. 2 out of 10 AD patients showed positive reaction to Chungkukjang with aloe extract. In conclusion, Chungkukjang could be recommended as functional food with hypoallergic effect. As adding aloe extract to Chungkukjang, hypo allergic effect was increased.

Ion Exchange of Ultrafiltrated Soybean Cooking Water for the Production of Soy-oligosaccharides (대두올리고당 생산을 위한 한외여과 대두침출액의 이온교환)

  • Ku, Kyung-Hyung;Park, Dong-June;Mok, Chul-Kyoon
    • Korean Journal of Food Science and Technology
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    • v.27 no.3
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    • pp.313-317
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    • 1995
  • Ion exchange process was optimized to purify ultrafiltrated bean cooking water(BCW) for the production of soy-oligosaccharides. The ultrafiltrated BCW with cutoff MW(COMW) 20,000 membrane was treated with various ion exchange resins. Protein and ash were mostly removed by anion and cation exchange resins, respectively. Based upon removing capabilities for ash and protein, a cation exchange resin(SK1B) and an anion exchange resin(WA30) were selected. Protein and ash were more efficiently removed at low extract/resin ratios(ERR), but part of the oligosaccharides were concomitantly lost. When 2-step-ultrafiltrated BCW first with COMW 20,000 membrane and successively with COMW 5,000 membrane was treated with a mixed resin(SK1B : WA30 =1 : 2) at ERR 5.0, most oligosaccharides were recovered in a clear protein- and ash-free liquid.

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Physiological and biochemical studies on legunme nodule bacteria, Rhizobia -I. Some charateristics of isolated strains of Rhizobia and inoculation test on soy bean. (두과작물 근류균에 대한 생리 및 생화학적 연구 -I. 근류균 균주의 특성과 접종시험-)

  • Lim, Sun-Uk
    • Applied Biological Chemistry
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    • v.13 no.1
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    • pp.51-57
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    • 1970
  • On the basis of the specific interrelationship between the species or variety of leguminous crops and the species or strain of nodule bacteria, Rhizobia, the rhizobial species and strain must be effectively chosen for the successful inoculation. The present paper describes on some results of the isolation and taxonomic study on the native rhizobial strains isolated from the nodules of five species of leguminous crops such as numerous varieties of soy bean, lespedeza, birdfoot trefoil, ladino and red clovers. The isolated strains of soy bean nodule bacterium, Rhizobium japonicum were grouped through the inoculation test on variety Changdanbaikmock into the effective, noneffective and toxic strain for the nodule formation. In the study of the effect of some inorganic and organic nitrogenous compounds on the growth of Rhizobium japonicum strain Ac 20, a promotive response was showed by asparagine, and glutamine, but hydroxylamine, nitrite, hydrazine and azide was inhibitory at the concentration of $10^{-2}M/l$ in mannitol-yeast extract basal medium. In the physiological characteristics each strain showed somewhat different activities of the indole-3-actic acid formation and hydrogenase and discussed with these characters in relation to nodule forming ability.

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Research regarding curcumin and soybean extract's influence on anti-inflammatory and acne and skin-repairing effect on RAW 264.7 macrophages (커큐민과 대두 추출물의 RAW 264.7 대식세포에서의 항염증 효과 및 여드름 피부 개선효과에 관한 연구)

  • Youa, Seon-hee;Moon, Ji-sun
    • Journal of the Korean Applied Science and Technology
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    • v.33 no.2
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    • pp.311-323
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    • 2016
  • By studying bioactivity in the aspect of skin care via curcumin, the main component of turmeric, and isoflavones, the soybean extract, this research tried to prove the possibilities as cosmetic ingredients. This research identified the curcumin and soybean extract's anti-inflammatory and toxic effects on skin cells through cell experiment, and using HPLC, the moisture, oiliness, and changes in erythema were measured when the soybean extract was applied to the skin. The result showed that curcumin had the least toxin on RAW 264.7 cell and the anti-inflammatory effect was identified. There were statistically significant changes on the moisture, oiliness and erythema when cosmetics containing curcumin and soybean extract were used. Therefore, it showed the possibilities of the utilization of curcumin and soybean extract to be used as cosmetics for acne skin.

Fungal bioconversion of Korean food wastes for the production of animal feed additive enzymes

  • Jeong, Yun-Seung;Jeong, Sang-Won;Jo, A-Ra;Gwon, Sun-U;Han, Seung-Ho
    • 한국생물공학회:학술대회논문집
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    • 2001.11a
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    • pp.529-532
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    • 2001
  • Korean food waste, one of the abundantly available but environmentally problematic organic wastes in Korea, was utilized as solid-substrate by fungal strain Aspergillus niger ATcC 6275 for the production of enzymemixture containing amylase, cellulase and xylanase. The enzyme mixture can be used as high value-added animal feed. Solid-state fermentation method yielded a 84-fold enhancement in xylanase activity compared with submerged fermentation method. The effect of incubation period, incubation temperature, pH of medium, moisture content, inoculum size and enrichment of the medium with nitrogen and carbon sources were observed for optimal production of these enzymes The optimal amylase activity of 33.10 U/g, cellulase activity of 24.41 U/g, xylanase activity of 328.84 U/g were obtained at 8 days incubation with 50%(w/w) soy bean flake, with incubation temperature of $25^{\circ}C$, pH of 6.38, optimal moisture content of 55% and with inoculum size of $3.8{\times}10^6$spore/g. Enzyme activities were enhanced when ImM $CaSO_4$, 2% Malt extract and 2% galactose were added as mineral, nitrogen and carbon enrichment respectively.

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