• Journal of Environmental Impact Assessment
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• v.23 no.2
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• pp.119-136
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• 2014

This work aims to select the potentially vulnerable plant species against climate change at alpine and subalpine belts of Mts. Sorak, Jiri, and Halla, from central, southern, southern insular high mountains of the Korean Peninsula, respectively. The selection of global warming related vulnerable plants were performed by adapting various criteria, such as flora, endemicity, rarity, floristically specific and valuable species, species composition at mountain summits, horizontal and vertical ranges of individual species, and their distributional pattern in the Korean Peninsula. Line and quadrat field surveys along the major trails from all directions at height above 1,500 meters above sea level of Mts, Sorak, Jiri and Halla were conducted each year during spring, summer, and autumn from 2010 to 2011. Based upon above mentioned eight criteria, high level of climate change related potentially vulnerable arboral plants, such as Rhododendron aureum, Taxus caespitosa, Pinus pumila, Oplopanax elatus, Vaccinium uliginosum, and Thuja koraiensis are noticed from at subalpine belt of Mt. Sorak. Species of Abies koreana, Rhododendron tschonoskii, Oplopanax elatus, Taxus cuspidata, Picea jezoensis, and Juniperus chinensis var. sargentii belong to climate change concerned vulnerable species at subalpine belt of Mt. Jiri. High level of climate change related species vulnerability is found at alpine and subalpine belts of Mt. Halla from Diapensia lapponica var. obovata, Salix blinii, Empetrum nigrum var. japonicum, Vaccinium uliginosum, Juniperus chinensis var. sargentii, Taxus cuspidata, Rhamnus taquetii, Abies koreana, Hugeria japonica, Prunus buergeriana, and Berberis amurensis var. quelpartensis. Countermeasures to save the global warming vulnerable plants in situ are required.

• Applied Biological Chemistry
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• v.40 no.4
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• pp.271-276
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• 1997

We have prepared several chimeric constructs containing the bacteriophage T7 RNA polymerase gene under control of the wound-inducible potato proteinase inhibitor II (pin2) promoter and have transformed Nicotiana tabacum plants with these constructs. Southern blot analyses indicate that either one or two copies of the gene constructs are present in the transgenic plants. Northern blot analyses indicate that mRNA encoding T7 RNA polymerase is expressed in a wound-inducible manner. We purified T7 RNA polymerase and prepared antiserum. This antiserum was used for Western blot analyses to demonstrate that a protein which is cross reactive with T7 RNA polymerase is produced. The molecular mass of this protein is 80 kDa, a size which is consistant with the nicked form of the polymerase as is often seen when expressed in E. coli. RNA polymerase assays were used to indicate that the nicked form of T7 RNA polymerase is active and capable of incorporating labeled nucleotides into transcripts in vitro. Analysis of transgenic plants did indeed show that wound-inducible activation of the T7 RNA polymerase permits the establishment of a genetic system to overexpress genes in plants using T7 RNA polymerase(Received March 20, 1997; accepted May 2, 1997)

• Journal of Plant Biotechnology
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• v.43 no.3
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• pp.341-346
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• 2016

The objectives of this study were to 1) evaluate the efficiency of the protocol of Agrobacterium-mediated transformation of cucumber to introduce phytoene synthase-2a carotene desaturase (PAC genes); 2) demonstrate the integration of PAC genes into the genome of putative transgenic cucumber based on growth on selection medium, PCR and Southern analysis; 3) evaluate the expression of PAC genes in transgenic cucumber based on the analysis of RT-PCR and Northern blot hybridization. Out of 5,945 cotyledonary-node explants inoculated with Agrobacterium, 65 (1.1%) explants produced 238 shoots. Integration of PAC genes into the genome of the cucumber was demonstrated based on the analysis of gDNA-PCR, 21 out of the 238 plants regenerated; while 6 plants proved positive for Southern blot hybridization. Transgene expression was demonstrated based on analysis of RT-PCR, 6 plants proved positive out of the 6 plants analyzed; while 4 plants out of 6 proved positive during Northern blot hybridization. This study successfully demonstrated the production of transgenic cucumber, integration, and expression of the PAC gene in cucumber.

• Plant Resources
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• v.6 no.1
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• pp.57-75
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• 2003

This study was performed to investigate the flora and plant resources of Mt. Woosanbong (537.8 m) from April to October 2002. The collected vascular plants were composed of all 389 taxa including cultivated species, and classified into 329 species, 2 subspecies, 53 varieties, and 5 forms of 248 genera under 81 families. Six taxa of the Korean endemic plants and 4 taxa of the rare and endangered plants were also distributed in this mount. Resource plants were categorized into edible 181, pasturing 160, medicinal 136, stainable 94, ornamental 77, timber 22, fiber 5 and industrial 4 taxa, respectively. Floristic geography of the investigated area was regarded as the boundary between middle and southern parts in floristic pattern of the Korean Peninsula.

• The Plant Pathology Journal
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• v.23 no.4
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• pp.266-271
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• 2007

The genomes of different allexiviruses were isolated and cloned from virus-infected garlic plants (Allium sativum), which were collected from farm fields in the southern provinces in Korea. The partial nucleotide sequences of the genomes from different allexiviruses were clearly identified in the virus-infected garlic plants. The cloned partial genomes of viruses in garlic plants showed a greater than 90% homology to previously identified allexiviruses and classified into species of GarV-A, -B, -C, -D, -E, and -X, demonstrating that species of allexivirus found in the other countries in the world are also widely distributed in the garlic plants in Korea.

• Proceedings of the Plant Resources Society of Korea Conference
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• 2010.05a
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• pp.7-7
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• 2010

Korea is one of the Northeast Asian countries in the Northern climatic zone. It is wide spread from north to south so that can be affected by various kinds of plants flora of the continent of Asia. Korea has complicated topography, mountains and hills included by an alpine belt. According to the distribution of plants, it can be classified to 5 areas; Northern part, Central part, Southern part, JeJuDo(濟州道) and UlLungDo(鬱陵島). Nakai of Japan, reported Plants in Korean peninsula as 3176 species, 841 varietal species and 174 varieties in "A Synoptical sketch of Korean flora". Lee of Korea, reported 3409 species, 6 sub-species, 756 varietal species and 287 varieties in "Korean Plants Resources". Isidoja(石戶谷) of Japan, simply described crude drug names, scientific names, effects, etc. of 45 species of Korean Medicinal Plants in the book "Journal of Jo-Seon Pharmacy(朝鮮藥學會會報)" third edition (published in 1925) and also explained 250 species of crude drug collected in Manchuria, Mongolia and Korean peninsula in the book "Medicinal plants in Northern Asia(北支那의 藥草)"(1931). Im and Jung organized 227 species of Medicinal Plants in "Wild Medicinal Plants from Jo-Seon(北支那의 藥草)" and it is said that 1000 species of plants can be used for medicinal purposes in Korea.

• Journal of the Korean Institute of Landscape Architecture
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• v.42 no.2
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• pp.33-40
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• 2014

The purpose of this study is to examine vegetation of the outer walls of the Jeongyi Town Wall in Jeju and analyze the characteristics and problems identified, for suggesting preliminary data for selecting species of plants appropriate for the greening of the walls in the southern temperate climate region, including Jeju. The result of this study is as follows. The number of plants growing naturally around the walls of the town was identified to be 52 taxa. Based on the list of naturalized plants, there are 5 taxa; Sonchus oleraceus, Houttuynia cordata, Crassocephalum crepidioides, Erigeron annuus and Lamium purpureum. The number of species by district was from 3 to 14 taxa with 7.1 taxa on average. Analyzing by the constancy class, plants in class III included Sedum bulbiferum, Trichosanthes kirilowii, Hedera rhombea and Boehmeria nivea. Manipulation of the species composition table shows that the number of plant species growing naturally around the walls of Jeongyi Town Wall is a total of 52 taxa, including 11 taxa by differential species of community and 41 taxa by companion species. The types of plants very useful for the covering of the walls are evergreen climbing vine, such as Hedera rhombea, Ficus thunbergii and Euonymus fortunei and deciduous climbing vine, such as Parthenocissus tricuspidata, Trichosanthes kirilowii and Paederia scandens. In addition, Ficus stipulata is identified as a vegetation more appropriate for the southern-ward lattice-blocked walls. Woody plants, such as Akebia quinata, Celastrus flagellaris, Ampelopsis brevipedunculata for. citrulloides, Rubus hirsutus, Clematis apiifolia and herbaceous plants, such as Dioscorea tenuipes, D. quinqueloba, D. nipponica, Cayatia japonica and Paederia scandens var. angustifolia are highly useful materials for climbing plants for covering the walls of the southern province. Pteridophyte, such as Lemmaphyllum microphyllum, Pteris multifida, Cyrtomium falcatum and Lygodium japonicumare suggested as very useful for increasing unique regional characteristics of the southern province, including Jeju.

• Journal of Plant Biotechnology
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• v.34 no.1
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• pp.31-36
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• 2007

Though higher plants car not metabolize D-amino acid, many prokaryotes and eukaryotes have the D-amino acid metabolism. Therefore, we transformed tobacco plants with D-amino acid oxidase (DAO), which can metabolize D-amino acid, and confirmed that transgenic tobacco plants might metabolize D-amino acid. Transgenic tobacco plants were survived a high concentration of D-serine, however non-transgenic plants were not grown on D-serine medium. From Southern and Northern blot analysis, transgenic tobacco plants selected on D-serine medium were confirmed by insert and expression of transgene. $T_{1}$ tobacco seeds derived $T_{0}$ tobacco plants selfing were grown on D-serine medium and showed normal phenotype compared to wild tobacco plants. Transgenic tobacco plants displayed the metabolic capability of D-serine. Therefore, we suggested that DAO is useful selectable marker gene for plant transformation.

• Proceedings of the Korean Society of Crop Science Conference
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• 2017.06a
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• pp.98-98
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• 2017

Leaf senescence is the process of aging in plants. Chlorophyll degradation during leaf senescence has the important role translocating nutrients from leaves to storage organs. The functional stay-green with slow leaf yellowing and photosynthesis activity maintenance has been considered one of strategy for increasing crop productivity. Here, we have identified two QTLs on chromosome 9 and 10 for leaf senescence with chlorophyll content of RIL population derived from a cross between Hanareum 2, early leaf senescence Indica-type variety, and Unkwang, delayed leaf senescence Japonica variety. Among these QTLs, we chose qPLS1 QTL on chromosome 9 for further study. qPLS1 was found to explain 14.4% of the total phenotypic variation with 11.2 of LOD score. Through fine-mapping approach, qPLS1 QTL locus was narrowed down to about 25kb in the marker interval between In/del-4-7-9 and In/del-5-9-4. There are 3 genes existed within 25kb of qPLS1 locus: LOC_Os09g36200, LOC_Os09g36210, and LOC_Os09g36220. Among these genes, transcript level of LOC_Os09g36200 was increased during the leaf senescence stage and the expression level of LOC_Os09g36200 in Indica was higher than in Japonica. Finally, we chose LOC_Os09g36200 as candidate gene and renamed it as OsPLS1-In and OsPLS1-Jp from Indica- and Japonica-type rice, respectively. OsPLS1-In and OsPLS1-Jp overexpressing transgenic plants showed both early leaf senescence phenotype. These results indicate that OsPLS1 functions in chlorophyll degradation and the difference of expression level of OsPLS1 cause the difference of leaf senescence between Indica and Japonica in rice.

• Korean Journal of Environment and Ecology
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• v.3 no.1
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• pp.16-27
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• 1989

The flora of Kayasan National Park which was located at the southern part of Korea was investigated between April 30-31. August 10-14, and October 9-10. 1989. The number of vascular plants investigated in the present study was summarized as 96 families, 292 genera, 465 species, 72 varities, and 4 forms. The middle and Southern types of distribution pattern were the largest as of 9 families and 12 species. It was very interesting that the warm temperate types of species, as well as southern coast and island types of plant were introduced to this region.