• Korean Journal of Plant Tissue Culture
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• v.21 no.6
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• pp.319-326
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• 1994

For the gene tagging of Armoracia rusticana, maize controlling element Ac and Ds were introduced into A.rusticana via Agrobacterium-mediated transformation method. We established an efficient in via regeneration and transformation system for gene transfer in A. rusticana. The optimum in via regeneration condition has been obtained from leaf, petiole and root organs on modified MS medium supplemented with NAA 0.1 mg/L plus BA 1.0 mg/L for direct shooting and with free growth regulators for root induction for transformation, the leaf, petiole and root explants of A. rusticana were concultivated with Agrobacterium tumefaciens, LBA4404 which carries a binary vector pEND4K containing maize controlling element Ac or Ds, respectively: Selections were performed in the shoot induction medium supplemented with 100 mg/L kanamycin, and 500 mg/L carbenicillin transformation frequency showed about 8 to 10% in case of leaf disks. PCR md Southern blot analyses showed that the Ac and the Ds elements were integrated into the chromosome of donor plants.

• Journal of Plant Biology
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• v.38 no.2
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• pp.203-209
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• 1995

Tobacco (Nicotiana tahacum L. cv. Wisconsin 38) leaf discs were cocultivated with Agrohacterium carrying a leghemoglobin (Lb) cDNA from Canavalia lineata. Seven plants were regenerated from the transformed leaf discs on MS media supplemented with 0.5 mg/L BAP, 0.1 mg/L ${\alpha}-NAA$, 200 mg/L kanamycin and 500 mg/L carbenicillin. Southern hybridization and PCR of genomic DNA from transgenic plants showed that the Lb cDNA was stably integrated into the genome of the tobacco. Total RNA from the transgenic tobacco showed northern hybridization signal at 1,000 nt and PCR of the first strand cDNA synthesized from the total RNA amplified 0.5 kb Lb cDNA. Furthermore, western hybridization using a polyclonal antibody against soybean Lb showed a 15.8 kD LB-like band on SDS-PAGE of proteins from the transformed tobacco. These results demonstrated that the Lb cDNA of C. lineata was not only incorporated into the genome of tobacco, but also transcribed into mRNA and translated into Lb protein in the transformed tabacco.

• Proceedings of the Korean Society of Crop Science Conference
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• 2017.06a
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• pp.142-142
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• 2017

Wheat (Triticum asetivum L.) is one of the major grain crops worldwide. The reduced productivity ascribed by adverse environment is increasing the risk of food security. Wheat cultivars have been actively released by public side since 1960s in Korea. Each variety has been developed for superior regional adaptation, pest resistance and mostly high yield. Heat stress tolerance is one of the major parameters that threaten wheat production in Korea. Heat stress during grain filling period has been conceived as critical level and directly influences on wheat production. We evaluated 11 common wheat cultivars ("Baegjoong", "Dajung", "Goso", "Hanbaek", "Jokyoung", "Joeun", "Jopum", "Keumgang", "Olgeuru", "Sinmichal", "Uri") that were exposed to abnormally high temperature during the grain filling period. Each plant was grown well in a pot containing "Sunshine #4" soil in controlled phytotron facility set on $20^{\circ}C$ and 16 h photoperiod. At 9 day-after-anthesis (DAA9), plants were subjected to a gradual increase in temperature from $20^{\circ}C$ to $33^{\circ}C$ and maintained constantly at $33^{\circ}C$ for 5 days. After the treatment, plants were subjected to gradual decrease to normal temperature ($20^{\circ}C$) and continue to grow till harvest. Seeds were harvested from each tiller/plant. Total chlorophyll contents decrease level as well as grain parameters were measured to evaluate varietal tolerance to heat stress. We also divide each spike into five regions and evaluate grain characteristics among the regions in each spike. The obtained results allow us to classify cultivars for heat stress tolerance. The pedigree information showed that typical wheat lines provide either tolerance or susceptible trait to their off-springs, which enable breeders to develop heat stress tolerance wheat by appropriate parental choice.

• Journal of agriculture & life science
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• v.46 no.4
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• pp.93-99
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• 2012

This study was conducted to investigate photosynthetic characteristics of two-year-old of Adenophora triphylla var. japonicum grown under control (full sunlight) and three different shading treatment (25, 50, and 75% shading treatment). Total chlorophyll contents like chlorophyll a and b content had not significant difference among treatments. Net photosynthetic rate of control and 25% treatment were higher than 50% and 75% treatment. Seedlings grown under full sunlight showed the highest photosynthetic activity such as photosynthetic rate, stomatal conductance and intercellular $CO_2$ concentration except for water use efficiency which was relative higher under 50% and 75% treatment.

• Asian Journal of Turfgrass Science
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• v.25 no.1
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• pp.11-16
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• 2011

This study was conducted to identify the morphological characteristics and variations level by site environment of native bermudagrass (Cynodon spp.) from costal and island region in South Korea. Soil chemical properties and morphological characteristics were investigated. There were significant differences in plant height, leaf width, leaf length, cotton on leaf blade, stolon, and number of seed per spike depending on where they were collected from natural habitat. We have discovered a variety of variation among the investigated traits in Korean native bermudagrass ecotypes. There were broad leaf and fine leaf types of native bermudgarass in S. Korea. Adaptability was in very high on inadequate environment in bermudagrass with broad leaf types, it has also been suggested that management worth in terms of use. These results may provide basic information for bermudagrass breeding development and the collected types during this investigation would be worth being preserved as genetic resources for further breeding purposes.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.49 no.2
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• pp.234-240
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• 2016

We examined the diffusion of contaminants released from the southern coast around Fukushima, Japan, during the passage of typhoons using a three-dimensional numerical model (POM) to track diffusing radioactivity (RA) released from the nuclear power plant at Fukushima following the accident caused by the giant tsunami event in March 2011. Radioactive contaminants released during the passage of typhoons may have significantly affected not only Japanese but also Korean coastal waters. The model domain covered most of the northwestern Pacific including marginal seas such as the East/Japan Sea and the Yellow Sea. Several numerical experiments were conducted case studies focusing on the westward diffusion from the southern coast of Japan of contaminants derived from the source site (Fukushima) according to various attributes of the typhoons, such as intensity, track, etc. The model produced the following results 1) significant amounts of contaminants were transported in a westward direction by easterly winds favorable for generating a coastal air stream along the southern Japanese coast, 2) the contaminants reached as far as Osaka Bay with the passage of typhoons, forced by a 5-day positive sinusoidal form with a (right-) northward track east of Fukushima, and 3) the range of contamination was significant, extending to the interior of the East/Japan Sea around the Tsugaru Strait. The model suggests that contaminants and/or radioactivity released from Fukushima with the passage of typhoons can affect Korean waters including the northeastern East/Japan Sea around the Tsugaru Strait, especially when the typhoon tracks are favorable for generating a westward coastal air stream along the southern Japanese coast.

• Nuclear Engineering and Technology
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• v.54 no.11
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• pp.4265-4271
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• 2022

Determination of ¹³⁷Cs, ⁴⁰K and ²¹⁰Po in water, bottom sediments and suspended matter of river systems of the Can Gio Biosphere Reserve, Vietnam was carried out. The average activity concentration of ¹³⁷Cs in waters of Ca Gau and Long Tau was 0.89 ± 0.14 and 1.08 ± 0.15 Bq m^-3 and was comparable to the levels of this radioisotope in waters of the East Sea. The activity concentration of ¹³⁷Cs in bottom sediments was 2.23 ± 0.81 and 3.63 ± 1.24 Bq kg^-1. The activity concentration of ¹³⁷Cs in water and bottom sediments could be characterized as low. So, the water areas of the Ca Gau and Long Tau rivers could be attributed to areas with insignificant pollution by technogenic radionuclides. The ²¹⁰Po activity concentration in bottom sediments of the Ca Gau and Long Tau rivers ranged from 9.2 ± 1.2 to 25.5 ± 2.1 Bq kg^-1, which is typical for river bottom sediments. Such values indicate the absence of anthropogenic enhancement of the entry of this radionuclide into the Can Gio river systems. The ⁴⁰K activity concentration varied within 467 ± 42-651 ± 39 Bq kg^-1 and represented typical values of potassium content in the bottom sediments of coastal water bodies, subject to a significant influence of the lithogenic component of suspended matter.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.48 no.5
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• pp.376-380
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• 2003

In order to examine the possibility that oil seed rape could be used as a forage fodder crop and to select the most suitable variety of forage rape at the southern area of Korea, two varieties of oil seed rape currently grown for oil production and six introduced varieties of forage rape with relatively high yield and high nutritional value were grown at the same condition their and yield components were observed. Forage rape was superior to oil seed rape in terms of yield components, plant fresh weight and plant dry mater weight. Velox was superior to any other variety of forage rape in these characters. When plant dry matter weight of the rape was subdivided into four components such as a main stem, branch stems, main stem leaves and branch leaves, contribution of these components to plant dry matter weight was in the order of branch stems, branch leaves, the main stem and main stem leaves. Dry matter percentage of the rape ranged from 9.32 to 11.08 percent, which was somewhat low value. There was no significant difference between two groups of the rape in terms of dry matter percentage. Velox showed somewhat higher value in dry matter percentage.

• Journal of Plant Biotechnology
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• v.41 no.1
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• pp.50-55
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• 2014

Plant-based vaccines possess some advantages over other types of vaccine biotechnology such as safety, low cost of mass vaccination programs, and wider use of vaccines for medicine. This study was undertaken to develop the transgenic maize as edible vaccine candidates for humans. The immature embryos of HiII genotype were inoculated with A. tumefaciens strain C58C1 containing the binary vectors (V662 or V663). The vectors carrying nptII gene as selection marker and scEDIII (V662) or wCTB-scEDIII (V663) target gene, which code EIII proteins inhibite viral adsorption by cells. In total, 721 maize immature embryos were transformed and twenty-two putative transgenic plants were regenerated after 12 weeks selection regime. Of them, two- and six-plants were proved to be integrated with scEDIII and wCTB-scEDIII genes, respectively, by Southern blot analysis. However, only one plant (V662-29-3864) can express the gene of interest confirmed by Northern blot analysis. These results demonstrated that this plant could be used as a candidated source of the vaccine production.

• Journal of The Korean Society of Grassland and Forage Science
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• v.25 no.4
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• pp.267-274
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• 2005

To develop transgenic orchardgrass (Dactylis glomerata L.) resistant to high temperature, a thermptolerance gene, DgP23, was introduced into orchardgrass using Agrobacterium - mediated transformation method. PCR and Southern blot analyses using genomic DNA showed specific DNA band on agarose gel and hybridization signal on X- ray film in transgenic orchardgrass harboring the recombinant DgP23 gene, but not in the wild type and empty vector control plants. RT-PCR and Southern blot analyses using total RNA also showed specific DNA band and hybridization signal. Transgenic orchardgrass did not showed ny morphological aberration both in the green house and field cultivation. Thermotolerance of transgenic plants was not detected in laboratory test. but may detected in field test.