• Journal of Ecology and Environment
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• v.29 no.4
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• pp.361-364
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• 2006

The objective of the current study was to characterize and apply multiple regression model relating to vegetation values of the plant species over salt marshes. For each salt marsh community, vegetation and soil variables were investigated in the western coast and the southern coast in South Korea. Osmotic potential of soil and $Cl^-$ content of soil as independent variable had positive and negative influences on vegetation values. Multiple regression model showed that vegetation values of 14 coastal plant communities were determined by pH of soil, osmotic potential of soil and sand content. The multiple regression equation may be applied to the explanation of distribution and abundance of plant communities with exiting ordination plots.

• Korean Journal of Breeding Science
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• v.51 no.4
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• pp.496-503
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• 2019

The soybean cultivar, 'Saegeum', has been developed for preparing soy-paste and tofu. The soybean cultivars 'Daepung' and 'SS98207-3SSD-168' were crossed in 2003 to obtain 'Saegeum'. Single seed descent method was used to advance the generation from F₃ to F₅, and the plant lines with promising traits were selected from F₆ to F₇ by pedigree method. The preliminary yield (PYT) and advanced yield trials (AYT) were conducted from 2009 to 2010, and the regional yield trial (RYT) was conducted in 12 regions between 2011 and 2013. The morphological characteristics of 'Saegeum' were as follows: determinate plant type, white flower, tawny pubescence color, and brown pod color. Flowering and maturity dates were August 2, XXXX and October 17, XXXX, respectively. Plant height, first pod height, number of nodes, number of branches, and number of pods were 79 cm, 18 cm, 16, 2.3, and 44, respectively. The seed characteristics of 'Saegeum' were as follows: yellow spherical shape, yellow hilum, and the 100-seed weight was 25.4 g. 'Saegeum' was resistant to bacterial pustule and SMV in the field test, and its lodging resistance was mildly strong, whereas its shattering resistance was excellent. The ability of this cultivar to be processed into tofu, soybean malt, and other fermented products was comparable with that of 'Daewonkong'. The yield of 'Saegeum' in the adaptable regions was 3.02 ton ha^-1. Thus, 'Saegeum' is adaptable to mechanized harvesting because of its high first pod height, as well as lodging and shattering resistance. (Registration number: 5929)

• Journal of Plant Biotechnology
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• v.34 no.3
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• pp.253-261
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• 2007

This study was conducted to develop an antibiotics marker-free potato (Solanum tuberosum L., cv. Taedong valley) plant having resistance against two herbicides. Agrobacterium tumefaciens strain EHA105, harboring a binary vector plasmid pCAMBIA3300 containing bar gene under the control of a promoter CaMV35S and linked CP4-EPSPS genes driven by CaMV35S promoter, was used in the current study. The leaf segments of newly bred potato variety (cv. Taedong Valley) was co-cultured with Agrobacterium. Then, the regenerated individual shoots were excised and transferred to potato multiplication medium supplemented with 0.5 mg/L phosphinothricin. The shoots were rooted in MS medium without hormone and obtained putative transgenic plant E3-6. Integration of target genes into the E3-6 plant and their expression was confirmed by PCR, Southern analysis, and ELISA test. The tissue necrosis test on young leaf blade and shikimic acid accumulation test using the tissue of E3-6 plant were conducted to investigate the resistance to glufosinate-ammonium and glyphosate, respectively. The transgenic plants (E3-6) simultaneously showed a high resistance to both herbicides. The same results were surely obtained also in the whole plants foliar-treated with alone or mixture of two herbicides, glufosinate-ammonium and glyphosate.

• Journal of Plant Biotechnology
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• v.29 no.2
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• pp.93-98
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• 2002

A VP6 fragments was subcloned with BamHI in the binary pMBP-1 vector under Califlower Mosaic Virus (CaMV) 355 promoter and neomycin phosphotransferase II (npt II) gene. The recombinant binary vector was mobilized into Agrobacterium-tumefaciens LBA4404 by the freeze-thaw method and potato (Solanum tubensum L. cv Desiree) was transformed by modified leaf-disc cocultivation. Shoots were induced on MS medium with 0.01 mg/L NAA, 0.1 mg/L GA$_3$, 2.0 mg/L Zeatin, 100.0 mg/L kanamycin, 500.0 mg/L carbenicillin. In order to identify the copy number of VP6 into potato plant, total genomic DNA was isolated from transgenic potato and analysed by Southern blotting. Genomic DNA and total mRNA analysis demonstrated the incorporation of the foreign gene into the potato genome, as well as their transcription.

• Journal of Ecology and Environment
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• v.29 no.6
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• pp.489-494
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• 2006

For the ecosystem conservation and the effective management of naturalized plant species on 261 uninhabited islands distributed in southern and western sea in Korea, we tested the interrelationship between the pattern of distribution and dispersal of the naturalized plant species and the factors of geographical environment of uninhabited islands such as island area, distance from mainland, latitude, longitude, human impacts and habitat diversity. Out of 261 uninhabited islands surveyed, 106 species of the naturalized plants occurred on 229 (87.7%) islands. The naturalized plant species per island averaged 4.6 (SD=4.07) species, and plant species number were higher in southern group (87 species) than in western group (64 species) of islands. Plant species occurred on more than 100 islands were three species : Rumex crispus L. (153 islands), Erigeron annuus Pers. (130 islands), and Conyza canadensis (L.) Cronquist (121 islands), the highest grade '5' of 'degree of naturalization'. The occurrence frequency of 106 naturalized plant species was significantly correlated with island area, distance to mainland, strength of human impacts, and latitude and longitude. Correlation coefficient (r=-0.330) between occurrence frequency and distance from mainland was higher than between occurrence frequency and island area (r=-0.182). The result of path analysis confirmed that plant species number was significantly affected by island area p=0.336) and distance from mainland (p=-0.490), but in this analysis the effect of human impacts on plant species number was very weak (p=0.003). On the basis of these results, strategies on the conservation of ecosystem and the management of naturalized plant species in the uninhabited islands of Korea were discussed.

• Proceedings of the Plant Resources Society of Korea Conference
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• 2003.04a
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• pp.126-126
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• 2003

Sucrose-phosphate synthase (SPS) is a key regulatory enzyme in sucrose synthesis. To investigate the role of SPS in carbon partitioning, we produced transgenic rice plants overexpressing a cyanobacterial SPS from Synechocystis sp. PCC 6803. The gene was expressed under the control of the maize Ubil promoter in transgenic plants. Southern and Northern blot analyses confirmed the integration and the expression of the transgene in four transgenic rice lines. All of the four transgenic! lines analyzed showed abnormal vegetative and reproductive developments. Analysis of SPS activities and primary metabolites in the transgenic rice plants will be presented.

• Mycobiology
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• v.32 no.3
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• pp.123-127
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• 2004

Sweet persimmons have been increasingly cultivated in the southern part of Korea. However, anthracnose disease caused by Colletotrichum species is one of the major hindrances in cultivation and productions. In this study, we used polymerase chain reaction(PCR) to detect Colletotrichum species with the AFLP(amplified fragment length polymorphism) method. In AFLP, we used E3(5'-GACTGCGTACCAATTCTA-3') and M1(5'-GATGAGTCCTGAGTAACAG-3') primer combination and, as a result, 262 bp segment was observed in Colletotrichum species only. Specific PCR primers were designed from the sequence data and used to detect the presence of the fungus in genomic DNA isolated from symptomless sweet persimmon plants. Based on sequence data for specific segments, Co.B1(5'-GAGAGAGTAGAATTGCGCTG-3') and Co.B2(5'-CTACCATTCTTCTA GGTGGG-3') were designed to detect Colletotrichum species. The 220 bp segment was observed in Colletotrichum species only, but not in other fungal and bacterial isolates.

• Proceedings of the Botanical Society of Korea Conference
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• 1999.04a
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• pp.14-14
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• 1999

• Journal of Plant Biotechnology
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• v.34 no.4
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• pp.323-329
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• 2007

Transgene expression was analyzed in tomato plants. Four lines of neomycin phosphotransferase II gene (NPTII) and the trehalose biosynthetic fusion gene (TPSP) transformed $T_0$ plants showed kanamycin resistance on selection medium. However, the analysis of phenotype (kanamycin resistance) and mRNA expression in $T_1$ plants indicated that the expression of the NPTII and TPSP transgenes was down-regulated to an undetectable level in two independent lines 1 and 11. Southern analysis demonstrated that the lines 1 and 11 had multicopies of the transgenes, whereas the typical transgenic lines 2 and 10 had 1 or 2 copies. DNA methylation analysis using methylation sensitive enzyme detected accumulated CpG DNA methylation on TPSP coding region and CaMV35S promoter region in the line 11, but not the typical transgenic line 2. These results suggest that multicopy transgene in plants is attributed to down-regulation of the transgene expression via transcriptional gene silencing.

• Korean Journal of Plant Resources
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• v.37 no.1
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• pp.71-78
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• 2024

This study was in order to select soybean (Glycine max (L.) Merrill) cultivars that are suitable for cultivating in paddy fields of the southern Korea and beneficial for mechanized harvesting with combine harvesters. Five soybean cultivars were cultivated in paddy fields to survey growth characteristics and seed yield, and evaluate the nutritional value of seed. Plant height was longer than 55 cm for 'Cheongja-3ho', 'Daewon' and 'Seonpung'. First pod height was higher than 12 cm for 'Daewon', 'Seonpung', and 'Seonyu-2ho'. Seed yields were 280 kg/10a or more for 'Daechan', 'Seonpung' and 'Daewon'. The protein content of 'Daechan' were 40.7%, which was significantly higher than those of the other cultivars, and isoflavone content was higher than 200 ㎍·g^-1 for 'Seonpung', 'Cheongja-3ho', 'Daechan', and 'Daewon'. These results suggest that 'Daechan', 'Seonpung', 'Daewon' could be suitable for cultivating in paddy fields, and 'Daewon' and 'Seonpung' could be more beneficial for mechanized harvesting with combine harvesters. Furthermore, 'Daechan' and 'Seonpung' have not only high seed yields, but also high protein and isoflavone contents, suggesting that they could be used for production of various food products.