• BMB Reports
• /
• v.41 no.1
• /
• pp.79-85
• /
• 2008

The source of nitric oxide (NO) in plants is unclear and it has been reported NO can be produced by nitric oxide synthase (NOS) like enzymes and by nitrate reductase (NR). Here we used wild-type, Atnos1 mutant and nia1, nia2 NR-deficient mutant plants of Arabidopsis thaliana to investigate the potential source of NO production in response to Verticillium dahliae toxins (VD-toxins). The results revealed that NO production is much higher in wild-type and Atnos1 mutant than in nia1, nia2 NR-deficient mutants. The NR inhibitor had a significant effect on VD-toxins-induced NO production; whereas NOS inhibitor had a slight effect. NR activity was significantly implicated in NO production. The results indicated that as NO was induced in response to VD-toxins in Arabidopsis, the major source was the NR pathway. The production of NOS-system appeared to be secondary.

• ETRI Journal
• /
• v.29 no.5
• /
• pp.655-666
• /
• 2007

There are nodes and edges in a topological map. Node data has been used as a main source of information for the localization of mobile robots. In contrast, edge data is regarded as a minor source of information, and it has been used in an intuitive and heuristic way. However, edge data also can be used as a good source of information and provide a way to use edge data efficiently. For that purpose, we define a data format which describes the shape of an edge. This format is called local generalized Voronoi graph's angle (LGA). However, the LGA is constituted of too many samples; therefore, real time localization cannot be performed. To reduce the number of samples, we propose a compression method which utilizes wavelet transformation. This method abstracts the LGA by key factors using far fewer samples than the LGA. Experiments show that the LGA accurately describes the shape of the edges and that the key factors preserve most information of the LGA while reducing the number of samples.

• KSII Transactions on Internet and Information Systems (TIIS)
• /
• v.8 no.2
• /
• pp.524-539
• /
• 2014

To investigate schemes of secret key generation from Ultra-wideband (UWB) channel, we study a statistical characterization of UWB outdoor channel for a campus playground scenario based on extensive measurements. Moreover, an efficient secret key generation mechanism exploiting multipath relative delay is developed, and verification of this algorithm is conducted in UWB Line-of-sight (LOS) outdoor channels. For the first time, we compare key-mismatch probability of UWB indoor and outdoor environments. Simulation results demonstrate that the number of multipath proportionally affects key generation rate and key-mismatch probability. In comparison to the conventional method using received signal strength (RSS) as a common random source, our mechanism achieves better performance in terms of common secret bit generation. Simultaneously, security analysis indicates that the proposed scheme can still guarantee security even in the sparse outdoor physical environment free of many reflectors.

• KSII Transactions on Internet and Information Systems (TIIS)
• /
• v.8 no.10
• /
• pp.3557-3571
• /
• 2014

We develop a secret key generation scheme using phase estimation in ultra-wideband (UWB) wireless fading channels. Based on the reciprocity theorem, two terminals extract the phase of the channel as a common random source to generate secret bits. Moreover, we study the secret key rate by a pair of nodes observing correlated sources and communicating to achieve secret key agreement over public communication channels. As our main results, we establish a more practical upper bound from Cramer-Rao bound (CRB) and compare it with a universally theoretical upper bound on the shared maximum key rate from mutual information of correlated random sources. Derivation and numerical examples are presented to demonstrate the bound. Simulation studies are also provided to validate feasibility and efficiency of the proposed scheme.

• KSII Transactions on Internet and Information Systems (TIIS)
• /
• v.9 no.2
• /
• pp.620-636
• /
• 2015

Survivability is a necessary property of network system in disturbed environment. Recovery ability is a key actor of survivability. This paper concludes network survivability into a novel composite metric, i.e. Network Recovery Degree (NRD). In order to measure this metric in quantity, a concept of Source-Destination Pair (SD Pair), is created to abstract end-to-end activity based on end nodes in network, and the quality of SD Pair is also used to describe network performance, such as connectivity, quality of service, link degree, and so on. After that, a Survivability Test method in large scale Network based on SD pairs, called STNSD, is provided. How to select SD Pairs effectively in large scale network is also provided. We set up simulation environment to validate the test method in a severe destroy scenario and evaluate the method scalability in different large scale network scenarios. Experiment and analysis shows that the metric NRD correctly reflects the effort of different survivability strategy, and the proposed test method STNSD has good scalability and can be used to test and evaluate quantitative survivability in large scale network.

• Journal of Microbiology and Biotechnology
• /
• v.20 no.11
• /
• pp.1471-1480
• /
• 2010

An extremely thermostable xylanase gene, xynB, from the hyperthermophilic bacterium Thermotoga maritima MSB8 was successful expressed in Kluyveromyces lactis. The response surface methodology (RSM) was also applied to optimize the medium components for the production of XynB secreted by the recombinant K. lactis. The secretion level (102 mg/l) and enzyme activity (49 U/ml) of XynB in the optimized medium (yeast extract, lactose, and urea; YLU) were much higher than those (56 mg/l, 16 U/ml) in the original medium (yeast extract, lactose, and peptone; YLP). The secretory efficiency of mature XynB was also improved when using the YLU medium. When the mRNA levels of 13 characterized secretion-related genes in the K. lactis cultured in YLP and YLU were detected using a semiquantitative RT-PCR method, the unfolded protein response (UPR)-related genes, including ero1, hac1, and kar2, were found to be up-regulated in the K. lactis cultured in YLU. Therefore, the nutrient ingredients, especially the nitrogen source, were shown to have a significant influence on the XynB secretory efficiency of the host K. lactis.

• International Journal of Naval Architecture and Ocean Engineering
• /
• v.11 no.1
• /
• pp.70-81
• /
• 2019

An improved three dimensional Rankine source method is developed to solve numerically water wave problems in time domain. The free surface and body surface are both represented by continuous panels rather than a discretization by isolated points. The integral of Rankine source 1/r on free surface panel is calculated analytically instead of numerical approximation. Due to the exact algorithm of Rankine source integral applied on the free surface and body surface, a space increment free surface source distribution method is developed and much smaller amount of source panels are required to cover the fluid domain surface than other numerical approximation methods. The proposed method shows a higher accuracy and efficiency compared to other numerical methods for various water wave problems.

• Journal of Microbiology and Biotechnology
• /
• v.26 no.1
• /
• pp.139-144
• /
• 2016

Synthetic oscillators are gene circuits in which the protein expression will change over time. The delay of transcription, translation, and protein folding is used to form this kind of behavior. Here, we tried to design a synthetic oscillator by a negative feedback combined with a positive feedback. With the mutant promoter P_LacC repressed by LacIq and P_Lux activated by AHL-bound LuxR, two gene circuits, Os-LAA and Os-ASV, were constructed and introduced into LacI-deleted E. coli DH5α cells. When glucose was used as the carbon source, a low level of fluorescence was detected in the culture, and the bacteria with Os-ASV showed no oscillation, whereas a small portion of those carrying Os-LAA demonstrated oscillation behavior with a period of about 68.3 ± 20 min. When glycerol was used as the carbon source, bacteria with Os-ASV demonstrated high fluorescence value and oscillation behavior with the period of about 121 ± 21 min.

• Journal of Power Electronics
• /
• v.13 no.3
• /
• pp.400-408
• /
• 2013

This paper presents a discrete state-space controller using state feedback control and feed-forward decoupling to provide a desirable control bandwidth and control stability for dynamic voltage restorers (DVR). The paper initially discusses three typical applications of a DVR. The load-side capacitor DVR topology is preferred because of its better filtering capability. The proposed DVR controller offers almost full controllability because of the multi-feedback of state variables, including one-beat delay feedback. Feed-forward decoupling is usually employed to prevent disturbances of the load current and source voltage. Directly obtaining the feed-forward paths of the load current and source voltage in the discrete domain is a complicated process. Fortunately, the full feed-forward decoupling strategy can be easily applied to the discrete state-space controller by means of continuous transformation. Simulation and experimental results from a digital signal processor-based system are included to support theoretical analysis.

• Nuclear Engineering and Technology
• /
• v.54 no.10
• /
• pp.3803-3810
• /
• 2022

OpenMC is a community-driven open-source Monte Carlo neutron and photon transport simulation code. The Weight Window Mesh (WWM) function and an automatic Global Variance Reduction (GVR) method was recently developed and implemented in a developmental branch of OpenMC. This WWM function and GVR method broaden OpenMC's usage in general purposes deep penetration shielding calculations. However, the Local Variance Reduction (LVR) method, which suits the source-detector problem, is still missing in OpenMC. In this work, the Weight Window Generator (WWG) function has been developed and benchmarked for the same branch. This WWG function allows OpenMC to generate the WWM for the source-detector problem on its own. Single-material cases with varying shielding and sources were used to benchmark the WWG function and investigate how to set up the particle histories utilized in WWG-run and WWM-run. Results show that there is a maximum improvement of WWM generated by WWG. Based on the above results, instructions on determining the particle histories utilized in WWG-run and WWM-run for optimal computation efficiency are given and tested with a few multi-material cases. These benchmarks demonstrate the ability of the OpenMC WWG function and the above instructions for the source-detector problem. This developmental branch will be released and merged into the main distribution in the future.