• 한국환경성돌연변이발암원학회지
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• 제17권1호
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• pp.7-11
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• 1997

Variations in adriamycin uptake and cytotoxicity were studied in tumor cells that were grown in different growth states and microenvironments. RIF-1 tumor cells were maintained in an RPMI 1640 medium, and grown in either a monolayer or multicell spheroids. For exponentially growing cells, adriamycin cytotoxicity increased with increased dosage up to 2.5 $\mu$g/ml, and this cytotoxicity was reduced when the cells were grown in a plateau phase or in an acidic microenvironment (pH 6.6). This reduced cytotoxicity was correlated with the uptake of the drug. For multicell spheroids, the cytotoxicity of the drug was reduced dramatically, and this reduction was also correlated with a reduced uptake of the drug and an acidic pH inside of the spheroids. When the drug cytotoxicity was evaluated at different locations within the spheroids, the cells in the inner regions were least affected by the drug, suggesting that both an acidic microenvironment and noncycling plateau phase cells are contributing factors in decreasing the efficacy of the drug in an organized tissue, such as multicell spheroids.

• Biomolecules & Therapeutics
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• 제31권4호
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• pp.425-433
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• 2023

During liver injury, hepatic stellate cells can differentiate into myofibroblast-like structures, which are more susceptible to proliferation, migration, and extracellular matrix generation, leading to liver fibrosis. Anaerobic glycolysis is associated with activated stellate cells and glyceraldehyde (GA) is an inhibitor of glucose metabolism. Therefore, this study aimed to investigate the anti-fibrotic effects of GA in human stellate LX-2 cells. In this study, we used cell viability, morphological analysis, fluorescence-activated cell sorting (FACS), western blotting, and qRT-PCR techniques to elucidate the molecular mechanism underlying the anti-fibrotic effects of GA in LX-2 cells. The results showed that GA significantly reduced cell density and inhibited cell proliferation and lactate levels in LX-2 cells but not in Hep-G2 cells. We found that GA prominently increased the activation of caspase-3/9 for apoptosis induction, and a pan-caspase inhibitor, Z-VAD-fmk, attenuated the cell death and apoptosis effects of GA, suggesting caspase-dependent cell death. Moreover, GA strongly elevated reactive oxygen species (ROS) production and notably increased the phosphorylation of ERK and JNK. Interestingly, it dramatically reduced α-SMA and collagen type I protein and mRNA expression levels in LX-2 cells. Thus, inhibition of ERK and JNK activation significantly rescued GA-induced cell growth suppression and apoptosis in LX-2 cells. Collectively, the current study provides important information demonstrating the anti-fibrotic effects of GA, a glycolytic metabolite, and demonstrates the therapeutic potency of metabolic factors in liver fibrosis.

• 문화기술의 융합
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• 제9권6호
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• pp.743-750
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• 2023

본 연구는 사회초년생들을 대상으로 직무소진에 미치는 긍정적인 요인과 부정적인 요인을 탐구하기 위해 수행되었다. 긍정적인 요인으로는 사회적 지원 및 공감을 선정하였고, 부정적인 요인으로는 직무권태와 직무 스트레스를 선별하였다. 이러한 요인들은 여러 연구에서 직무소진에 영향을 미치는 주요 원인으로 다루어졌다. 연구방법으로는 위계적 회귀분석을 이용하여 변수 간 영향력을 더욱 세밀하게 파악해 보았다. 연구결과 부정적 요인들(직무 스트레스, 직무권태)이 긍정적 요인들(공감, 사회적 지원)보다 직무소진에 더 많은 영향을 미치고 있었다. 이에 사회초년생들에게 관심과 배려가 절실히 필요하다는 것을 확인할 수 있었다.

• 센서학회지
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• 제11권2호
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• pp.93-101
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• 2002

농산물의 품질은 여러 가지 인자에 의해 결정되는데, 이 인자들은 파괴 또는 비파괴 측정방법을 이용하여 측정되고 검사된다. NIR분광분석법은 당도, 산도와 같은 내부품질을 측정하는데 이용되고 있다. 과일의 색, 크기와 같은-외부 품질 측정에는 CCD 카메라가 사용되고 있다. 오늘날 이러한 비파괴 검사법이 널리 연구되고 있다. 컵 위에 자동으로 탑재되어 카메라와 NIR장비로 실시간으로 측정된 과일의 품질과 등급은 내부 외부인자에 의해 결정된다. 본 논문은 과일 선별기를 제작함에 있어서 과일의 칼라영상의 경계를 검출하는 수정된 윤곽선 추적법과 일반적인 방법보다 빠른 속도로 연결점 데이타를 구성하는 방법을 제안한다. 연결점 데이타는 과일 영상의 크기 계산과 하나의 컵에 중복탑재 과일과 컵 사이에 불량 탑재된 과일을 검사하는데 사용된다. 설계된 과일 선별기는 귤의 경우 과인의 당도 산도 및 크기를 검사하면서 초당 8개의 과일을 선별할 수 있다.

• 한국과학교육학회지
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• 제18권4호
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• pp.503-516
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• 1998

본 연구는 형식적 조작기의 초기에 형성되며, 형식적 사고와 과학적 추론의 기초가 되는 비례 논리의 형성에 미치는 학습자 요인과 비례 논리 향상을 위한 프로그램의 적용효과를 분석하는데 그 목적이 있다. 비례 논리 형성에 미치는 요인 분석을 위하여 형식적 사고기의 초기에 해당하는 중학교 1, 2, 3학년 학생 790명을 대상으로 하였다. 표집된 학생들에게 과학적 사고력 검사, WCST, 설계기능검사, 정신용량 검사, 인지양식 검사, 뇌기능 분화 검사, 동시적/연속적 처리 검사, 과학적 추론 검사를 실시하였다. 한편, 비례논리 향상을 위한 프로그램의 적용을 위해서 비례논리가 형성되지 않은 학생 133명에게 처치하여 그 효과를 분석하였다. 본 연구의 결과 학습자의 연령, 과학적 추론 능력, 정신용량, 동시적/연속적 정보처리 점수가 높을수록 비례논리 형성 정도가 높았다. 반면에 보속적 오류가 적을수록 비례논리 형성 정도가 높았다. 비례논리 형성에 높은 예언력을 가지고 있는 대표적인 변인으로는 과학적 추론, 정보처리, 보속적 오류, 연령의 학습자 요인이다. 형식적 사고기의 연령에 있는 학생 중 비례논리가 형성되지 않은 학생을 위해서 비례 논리 형성을 위한 프로그램이 효과가 있는 것으로 나타났다. 본 연구결과를 바탕으로 논리적 사고 형성에 미치는 요인에 대한 분석과 비례논리의 형성이 기타의 논리 형성과 과학 교과목의 성취도에 미치는 영향에 대한 연구가 계속되기를 기대한다.

• 한국치위생학회지
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• 제9권1호
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• pp.125-139
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• 2009

Objective : This study was performed in order to provide basic data for halitosis prevention and establish a device to efficiently eliminate halitosis and to analyze the factors that affect the halitosis. Methods : Oral examination on the Gingival index, CPITN, Tongue Plaque index, and OHI-S as well as halitosis measurement among 293 rural residents. Results : Gingival index was high at mild on female and at moderate on male(p=0.025). Sorting the result by age, mild was 54.1% in the 40s, and moderate was 49.5%, 42.0% and 70.0% each in the 50s, 60s, and the 70s(p=0.005). The need for dental plaque management was 100%. The need for scaling was high with 78.3%, 93.0%, 89.9%, and 90.0% each for the 40s, 50s, 60s, and 70s. The need for complex periodontal treatment was also high with 32.4%, 47.5%, 48.7% and 60.0%, each for the 40s, 50s, 60s, and 70s(p=0.050). The highest bad condition for OHI-S was 69.5%, and good being 18.9%, and very bad being 11.6%. For moderate tongue plaque index was 74.4% as the highest. As the level of education increased, the slight tongue plague was increased, but in contrast, the moderate and higher tongue plague index was decreased(p=0.010). OG under 50ppm was 61.1% on male and OG over 50ppm was 50.9% on female(p=0.041). In OG over 50ppm, CPITN was 52.1% and 41.9% in scaling and complex periodontal treatment group(p=0.018). OHI-S, in bad and very bad condition with OG, over 50ppm, was 48.7% and 46.9%(p=0.019). The higher tongue plague index showed significant amount of increase at OG and EG above 50ppm(p=0.006). $NH_3$, as the tongue plaque index increased, the wider range of distribution was shown(p=0.000). As for the multiple regression analysis result, there have been selected females and tongue plaque index as factors affecting OG. There have been selected age and tongue plaque index as factors affecting EG and there have been selected females as factors affecting on $NH_3$. Conclusions : With the aforementioned results in mind, the status of halitosis among rural residents is considered to bare a close relation with oral environments. we have to focus on correct tooth brushing methods and tongue brushing, with using tongue cleaner to remove fur of tongue plaque. Also, in order to analyze exactly the factors of individual halitosis, we need continuous and systematic study.

• The Korean Journal of Physiology
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• 제30권2호
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• pp.197-208
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• 1996

Endosomes lower their internal pH by an ATP-driven proton pump, which is critical to dissociation of many receptor-ligand complexes, the first step in the intracellular sorting of internalized receptors and ligands. Endosomes are known to exhibit n great range of pH values that can vary between 5.0 and 7.0 within a single cell although the factors that regulate endosomal pH remain uncertain. To evaluate the morphological and topological differences of endosomes in the different stages, confocal microscopy was used. The early endosomes labeled with fluorescein isothiocyanate-dextran for 10 min at $37^{\circ}C$ were identifiable at the peripheral and tubule-vesicular endosome compartment. In contrast, the late endosomes formed by 10 min pulse and 20 min trace were located deeper in the cytoplasm and showed more vesicular features than early endosomes. For the purpose of determining whether ATP-dependent acidification was heterogeneous and whether the differences in acidification were attributed to differences in the activity of $Na^{+}-K^{+}$-ATPase and/or $Cl^{-}$ channel, endocytic compartments were fractionated into subpopulation using percoll gradient and measured ATP-dependent acidification. While all fractions exhibited ATP-dependent acidification activity, both the initial rate of acidification and extent of proton translocation were lower in early endosomes and gradually increased in late endosomes. Phosphorylation by PKA and ATP enhanced ATP-dependent acidification in both early and late endosomes, hut there was no difference in the degree of enhancement by phosphorylation between two subpopulations. When ATP-dependent acidification was determined in the presence or absence of vanadate ($Na_{3}VO_{4}$) or ouabain, only early endosomes exhibited the vanadate or ouabain dependent stimulation of acidification activity, suggesting the inhibition of $Na^{+}-K^{+}$-ATPase. Therefore, it seems probable that the inhibition of early endosome acidification by $Na^{+}-K^{+}$-ATPase observed in vitro at least in part plays a physiological role in controlling the acidification of early endosomes in vivo.

• Journal of Biosystems Engineering
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• 제26권3호
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• pp.271-278
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• 2001

Many workers in the garlic peeling factory are separating the sound peeled garlics from the unpeeled and defective ones in a manual way. In order to reduce the seasonal labor requirement and operating cost, the mechanized garlic sorting system such as the vision-based garlic sorter should be developed. This study was conducted as one of basic studies on developing quality evaluation technique for peeled garlics, especially to developed the system for acquiring the whole surface images of garlics with a CCD camera. The following results were obtained from this study. 1. The belt-type garlic rotation system was devised to apply for the vision-based garlic sorter and was tested to decide the criteria of design and optimum conveying speed. 2. To evaluate the performance of the developed garlic rotation system, feeding rate and rotating rate were measured under the conditions of four experimental factors such as the inclined angle of rotating belt, the inclined angle of feeding belt, the height of plate arrays on feeding belt and the conveying speed of belts. And the capacity of the system according to mixture ratios of peeled garlics and unpeeled garlics was analyzed as a feasibility test. 3. For the inclined angle of rotating belt 20°and height of plate array on feeding belt 22㎜, the maximum rotating rate for garlic samples including unpeeled ones was 81.1% at the conveying speed of 4.2 garlic/sec. And under these condition, the maximum feeding rate was 85% at the inclined angle of feeding belt 6.5°. 4. The capacity of the developed garlic rotation system was almost constant regardless of mixture ratio of peeled garlics and unpeeled garlics and its range was 2.95∼3.92 garlic/sec. At the conveying speed of 4.2 garlic/sec, the capacity of the garlic rotation system was calculated ad 58∼64 kg/hr. 5. To improve performance of the garlic rotation system, it is recommended to develop a device to slide garlics into feeding belt.

• 한국의류산업학회지
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• 제18권3호
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• pp.327-337
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• 2016

The purpose of this study is for size suitability and fitness improvement for Korean 20's women. This study has classified hip types of 20's women by the 6th Korean Anthropometric Survey(Size Korean), then compared their lower body-type by hip types each other. The results are as follows. Factor analysis in sorting hip types can be explained 77.72% by nine factors. And cluster analysis can be divided into three types. Type 1 is referred to as a general type which has the largest size in the 'Hip circumference', but the buttocks's horizontal sizes are in the middle size besides the hip line. Type 2 is referred to as a protruding type in upper buttocks which is large in protruding volume of upper buttocks, but the buttock's horizontal sizes are small including 'Hip circumference', and buttock's horizontal section form close to circle. Type 3 is referred to as a type which is contrary to Type 2. Type 3 is small in protruding volume of upper buttocks, but large in lower buttocks, and the protrusions in whole buttocks are small, so the side view of Type 3 made gentle fit shape. By these results, the study could classify lower body of 20's women, and it can be basic data for new sizing systems and pattern development of bottoms.

• 한국실내디자인학회논문집
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• 제22권2호
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• pp.58-67
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• 2013

The methods used in this study included investigation of media facades used for landmarks in downtown areas in previous studies, visits to these areas, and recording of media facades. The changes in the colors or the size of buildings were analyzed among the recorded cases and 12 of these cases were selected for further study. The sensibility preference of the media facades was evaluated by sorting the 12 kinds of media facades, and providing them as materials to a group of 60 participants (40 undergraduate and 20 graduate students majoring in architecture and design) consisting of an equal proportion of males and females. This study addressed the following four_stage questions: 1) Five questions of recognition evaluation about media facades and the cognitive evaluation items of emotional vocabulary and color preference in each research case; 2) sensibility preference items regarding the media facade color design; and 3) design evaluation items of the media facades; 4) Video clips and still images were recorded from a middle distance at 7p.m to 11p.m. in central New York, Singapore, Seoul, and Beijing. The participants looked at the changes in colors through the video clips in each case and evaluated their preferences through 23 pairs of emotional vocabulary items using system dynamics. Construction of an emotional vocabulary followed, based on previous studies about media facades and color design. To evaluate the sensibility preference and the perceived representative colors of the media facade, this study suggests new emotional responses that depended on the color emotional vocabulary of light in the LED lighting technical evaluation methodology. A media facade with a moving change of colors, unlike a fixed landscape color design, suggests a new communication method based on architectural factors. New architectural color coordination can be presented for urban landscapes at night. Designs that factor in the pedestrians' emotional vocabulary or preference should take precedence over the use of high luminance and various colors.