• Title/Summary/Keyword: Sorting System

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Optimization of Yeast Surface-Displayed cDNA Library Screening for Low Abundance Targets

  • Kim, Juhyung;Kim, Hyung Kyu;Jang, Hye Jeong;Kim, Eunkyung;Kim, Moon Kyu
    • Journal of Microbiology and Biotechnology
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    • v.25 no.4
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    • pp.547-553
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    • 2015
  • The yeast surface-displayed cDNA library has been used to identify unknown antigens. However, when unknown target antigens show moderate-to-low abundance, some modifications are needed in the screening process. In this study, a directional random-primed cDNA library was used to increase the number of candidates for the unknown antigen. To avoid the loss of target yeast clones that express proteins at a low frequency in the cDNA library, a comprehensive monitoring system based on magnetic-activated cell sorting, fluorescence-activated cell sorting, and immunofluorescence was established, and a small number of target yeast cells was successfully enriched. These results showed that our optimized method has potential application for identifying rare unknown antigens of the human monoclonal antibody.

A Study on Improvement and Evaluation Plan of the Korean Postal Code (우편번호 체계 개선 및 평가 방안 연구)

  • Chang, Tai-Woo;Wang, Seung-Jin;Lim, Joon-Mook;Kim, Ho-Yon;Bae, Sung-Min
    • IE interfaces
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    • v.19 no.3
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    • pp.236-244
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    • 2006
  • Postal code is essential for improving efficiency of mail sorting. The Korean postal code, which is introduced in 1970 and had been reformed two times, has controversial issues because of changed postal environments such as modification of administrative districts and logistical transformation into a hub-and-spokes structure. In this study, we propose several improvement and evaluation methods in order to prepare an alternative plan of postal code system. After performing status analysis, we define the customers of postal code and analyze the requirements of them. The 6 proposed new postal code systems are based on each customer groups’ requirements. To evaluate the new systems, we develop several quantitative and qualitative indexes and make a survey of postal experts. Besides, we evaluate the influence of them to the postal environments including delivery sequence sorting process.

Single Buffer types of ATM Switches based on Circulated Priority Algorithm (순환적 순위 알고리즘을 이용한 단일형 버퍼형태의 ATM스위치)

  • Park Byoung-soo;Cho Tae-kyung
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.5 no.5
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    • pp.429-432
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    • 2004
  • In this paper, we propose a new sorting algorithm for ATM switch with a shared buffer which has a sequencer architecture with single queue. The proposed switch performs a sorting procedure of ATM cell based on the output port number of ATM cell with hardware implementation. The proposed architecture has a single buffer physically but logically it has function of multi-queue which is designed at most to control the conflicts in output port. In the future, this architecture will take various applications for routing switch and has flexibility for the extension of system structure. therefore, this structure is expected on good structure in effective transmission.

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A Motion Analysis Study of Casers for Fish Boxes using Computer Simulation (컴퓨터 시뮬레이션을 이용한 어체 상자 제함기 동작 분석에 관한 연구)

  • Jung, Sung-Heon;Jun, Chul-Woong;Sohn, Jeong-Hyun
    • Journal of the Korean Society of Manufacturing Process Engineers
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    • v.18 no.4
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    • pp.56-61
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    • 2019
  • In this country, mackerel landing, sorting, and packing are mostly performed manually, which is time consuming and labor intensive. An unloading automation system saves time and labor by automating the landing, sorting, and packing processes. Casers are devices for manufacturing packing boxes for fish used by unloading automation systems. The caser design in this study is for mackerel packing boxes. This caser makes a packing box based on a press using the caser's slide crank. When the caser makes a packing box, the manufacturing sequence is determined by the caser's production guide and assisting rod. The caser design in this study is simulated using a multi-body dynamics program. The simulation is used to analyze the caser and to visualize the box-making sequence.

The Simulation Design and Analysis Method of Integrated Logistics System using an Aspect Oriented Approach (Aspect-Oriented Approach를 이용한 통합 물류 시스템의 시뮬레이션 설계 및 분석 방법)

  • Kim, Tae-Ho;Um, In-Sup;Lee, Hong-Chul
    • IE interfaces
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    • v.20 no.4
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    • pp.438-447
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    • 2007
  • This paper presents an aspect-oriented approach to simulation design and analysis in system design phase for integrated logistics system simulation. The integrated logistics system composed of AS/RS (Automated Storages and Retrieval System), AGVs (Automated Guided Vehicle System), STVs (Sorting Transfer Vehicle System) and Conveyor System is designed by using the aspect-oriented approach and UML (Unified Modeling Language). The multi-factorial design of experiments and regression analysis are used for design parameters of the system and Evolution Strategies is used to verify each parameter. Aspect-oriented approach for the integrated logistics system simulation shows the advantages of code reusability, extendible, modulation, easy improvement and a better design technique.

A study on Parallel Interference Cancellation scheme based sorting method for a Multi-carrier DS/CDMA System (MC-DS/CDMA 시스템에서 정렬기법을 이용한 병렬형 간섭제거기법의 성능개선에 관한 연구)

  • Park Jae-Won;Park Yong-Wan
    • Journal of the Institute of Electronics Engineers of Korea TC
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    • v.42 no.1
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    • pp.17-27
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    • 2005
  • In this paper, we introduce a Parallel Interference Canceller (PIC) based sorting method to improve performance in the MC-DS/CDMA environment. A conventional PIC estimates and subtracts out all of the MAI (Multiple Access Interference) for each user in parallel. The parallel process ensures the low delay for the detection of all users. Also this scheme requires more stages for having better performance. Since the performance of PIC is strongly related to the correct MAI estimation, we introduce the IC (Interference Cancellation) scheme to estimate the accurate weaker signal group than the desired signal using conventional PIC. The principle of the proposed receiver sorts in descending order by the strength of signal and subtracts the MAI of the strong interferers from the desired signal for the accurate estimate of the weaker signals. Following this, the proposed scheme cancels out the improved weaker interference from the desired signal, using the output of the pre-step. In this result, the proposed system obtains better BER performance than the conventional PIC because the accuracy of the strong signal is improved. However, a disadvantage exists in that the processing time has slightly longer delay than the PIC owing to the power sorting and the MAI estimation process. The system performance evaluates and compares other non-liner It according to the number of sub-carriers in the limited-bandwidth.

A Study of the Information Structuring of an Integrated Navigation System (INS) Based on User Experience using a Card Sorting Test (카드 소팅 분석을 통한 사용자 경험 기반의 통합항해시스템 정보 구성에 관한 연구)

  • Bora, Kim;Yun-sok, Lee;Young-Joong Ahn
    • Journal of the Korean Society of Marine Environment & Safety
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    • v.29 no.2
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    • pp.160-167
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    • 2023
  • An INS is a composite navigation system providing "added value" so defined if work stations provide Multi-Function Displays(MFDs) integrating information and functions for navigational tasks. Even though the minimum requirements for an INS are defined by IMO performance standards, a generic list of the devices and functions that constitute an INS does not exist, so the configuration of the INS is different for each manufacturer, and guidelines based on users' perspectives are also insufficient. This study was conducted to enhance the usability of the INS by analyzing the information required by users according to the ship's operating status and tasks and effectively structuring it in the MFD of the INS. By analyzing INS-related international standards and manufacturers' component equipment lists, mandatory navigation information was selected and card sorting tests were conducted on ship operators with experience in using MFDs to group the information required for each INS task. The results of the study can serve as a basic guideline for manufacturers to structure information based on users' experience when designing products.

A Study on the Export Logistics System Improvement of Fresh Agriculture Products (신선농산물 수출 물류시스템 개선에 관한 연구)

  • Roh, Kyung-Ho
    • Management & Information Systems Review
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    • v.24
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    • pp.155-185
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    • 2008
  • This study tries to find out the resonable improvement method of the export logistics system for fresh agriculture products. Export logistics system improvement of fresh agriculture products rationalized the export network covering the export and import of fresh agriculture products from producers to end customers and realized cost reduction and profit maximization. We are also operating our very own comprehensive logistics system that maximizes customer satisfaction. Supporting the entire logistics process starting from the collection, unloading, sorting, packaging, customs clearing, and information up to comprehensive logistics services, the export logistics system of fresh agriculture products realized a comprehensive transport system that uses 12 feet cool container. We have successfully reduced logistics costs through efficient operation in the export logistics system of fresh agriculture products.

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An optimal design of wind turbine and ship structure based on neuro-response surface method

  • Lee, Jae-Chul;Shin, Sung-Chul;Kim, Soo-Young
    • International Journal of Naval Architecture and Ocean Engineering
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    • v.7 no.4
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    • pp.750-769
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    • 2015
  • The geometry of engineering systems affects their performances. For this reason, the shape of engineering systems needs to be optimized in the initial design stage. However, engineering system design problems consist of multi-objective optimization and the performance analysis using commercial code or numerical analysis is generally time-consuming. To solve these problems, many engineers perform the optimization using the approximation model (response surface). The Response Surface Method (RSM) is generally used to predict the system performance in engineering research field, but RSM presents some prediction errors for highly nonlinear systems. The major objective of this research is to establish an optimal design method for multi-objective problems and confirm its applicability. The proposed process is composed of three parts: definition of geometry, generation of response surface, and optimization process. To reduce the time for performance analysis and minimize the prediction errors, the approximation model is generated using the Backpropagation Artificial Neural Network (BPANN) which is considered as Neuro-Response Surface Method (NRSM). The optimization is done for the generated response surface by non-dominated sorting genetic algorithm-II (NSGA-II). Through case studies of marine system and ship structure (substructure of floating offshore wind turbine considering hydrodynamics performances and bulk carrier bottom stiffened panels considering structure performance), we have confirmed the applicability of the proposed method for multi-objective side constraint optimization problems.