• Bulletin of the Korean Chemical Society
• /
• v.20 no.6
• /
• pp.689-695
• /
• 1999

Supercritical fluid extraction (SFE), ultrasonic extraction (USE), and accelerated solvent extraction (ASE) were compared with the well known Soxhlet extraction for the extraction of polychlorinated biphenyls (PCBs) and polychlorinated dibenzo-p-dioxins(PCDDs) from the XAD-2 resin which was used to adsorb PCDDs in the atmosphere. XAD-2 resin spiked with five PCDDs was chosen as a sample. The optimum conditions for the extraction of PCDDs by SFE were turned out to be the use of CO2 modified with 10% toluene at 100 ℃ and 350 atm, with 5 min static extraction followed by 20 min dynamic extraction. SFE gave a good extraction rate with good reproducibility for PCDDs ranging from 68 to 98%. The ultrasonic extraction of PCDDs from XAD-2 was investigated and compared with other extractions. A probe type method was compared with a bath type. Two extraction solvents, toluene and acetone were compared with their mixture. The use of their mixture in probe type, with 9 minutes of extraction time, was found to be the optimum condition. The average recovery of the five PCDDs for USE was 82-93%. Accelerated solvent extraction (ASE) with a liquid solvent, a new technique for sample preparation, was performed under elevated temperatures and pressures. The effect of tem-perature on the efficiency of ASE was investigated. The extraction time for a 10 g sample was less than 15 min, when the organic solvent was n-hexaneacetone mixture (1 : 1, v/v). Using ASE, the average recoveries of five PCDDs ranged from 90 to 103%. SFE, USE, and ASE were faster and less laborious than Soxhlet extraction. The former three methods required less solvent than Soxhlet extraction. SFE required no concentration of the solvent extracts. SFE and ASE failed to perform simultaneous parallel extractions because of instrumental limitations.

• Journal of the Korean Applied Science and Technology
• /
• v.38 no.1
• /
• pp.292-299
• /
• 2021

In this study, a study was attempted to confirm the yield of the solvent fraction for extracting physiologically active substances of polyphenols and flavonoids in sea cucumber. In the previously reported cases, the 50% ethanol extraction solvent fraction was confirmed as a result of high yield of sea cucumber antioxidants. The determination of the amount of antioxidants extracted from sea cucumber was confirmed as a result of the extraction solvent fraction applied to the extraction. In addition, the necessity of extraction of the solvent fraction was obtained through a previous study that 'there is a significant difference in the content of sea cucumber physiologically active antioxidants extracted depending on the extraction solvent.' The results of extracting high antioxidants from the phenolic substances contained in the sea cucumber extract of the 50% ethanol extraction solvent fraction were demonstrated. In the study case of extraction solvent fraction, the ethyl acetate solvent fraction, which yielded the lowest, yielded a higher phenol content than other solvent fractions, and the antioxidant effect was confirmed. Accordingly, it was confirmed that the effect on the high yield of the antioxidant extract through the yield change according to the application of the extraction solvent fraction was performed. Therefore, it was verified as an optimized sea cucumber physiologically active substance extraction solvent fraction of 50% ethanol solvent.

• The Korean Journal of Food & Health Convergence
• /
• v.5 no.5
• /
• pp.11-25
• /
• 2019

Extraction of oil from Moringa oleifera seed using Response Surface Methodology (RSM) was investigated. Effects of three factors namely: sample mass, particle size and extraction time on the response, Moringa oleifera a volume extracted, were determined. The Box-Behnken design of RSM was employed which resulted in 15 experimental runs. Extraction was carried out in a 250 ml Soxhlet extractor with Hexane and Ethanol as solvent. The Moringa oleifera seed powder was packed inside a muslin cloth placed in a thimble of the Soxhlet extractor. The extraction was carried out at 60℃ using thermostatic heating mantle. The solvent in the extracted oil was evaporated and the resulting oil further dried to constant weight in the oven. This study demonstrates that Moringa oleifera oil can be extracted from its seed using ethanol and acetone as extraction solvent. The optimum process variables for both solvent (ethanol and acetone) was determined at sample weight of 40 g, particle size of 325 ㎛ and extraction time of 8 hours. It can be deduced that using acetone as solvent produces a higher yield of oil at the same optimum variable conditions compared to when ethanol was used.

• Natural Product Sciences
• /
• v.25 no.2
• /
• pp.150-156
• /
• 2019

Conventional extraction of oil and azadirachtin, a botanical insecticide, from Azadirachta indica involves defatting the seeds and leaves using hexane followed by azadirachtin extraction with a polar solvent. In order to simplify the process while maintaining the yield we explored a binary extraction approach using Soxhlet extraction device and hexane and ethanol as non-polar and polar solvents at various ratios and extraction times. The highest oil and azadirachtin yields were obtained at 6 h extraction time using a 50:50 solvent mixture for both neem leaves (44.7 wt%, $720mg_{Aza}/kg_{leaves}$) and seeds (53.5 wt%, $1045mg_{Aza}/kg_{leaves}$), respectively.

• Food Science and Biotechnology
• /
• v.14 no.6
• /
• pp.836-840
• /
• 2005

Extraction characteristics of Bonus species of Brassica oleracea var. capita and functional properties of corresponding extract were monitored by response surface methodology (RSM). Maximum extraction yield of 44.07% was obtained at ratio of solvent to sample of 27.94 mL/g, ethanol concentration of 24.35%, and extraction temperature of $55.21^{\circ}C$. At ratio of solvent to sample, ethanol concentration, and extraction temperature of 21.11 mL/g, 58.53%, and $68.83^{\circ}C$, respectively, maximum electron-donating ability was 48.44%. Maximum inhibitory effect on tyrosinase was 68.94% at ratio of solvent to sample, ethanol concentration, and extraction temperature of 24.08 mL/g, 10.49%, and $78.71^{\circ}C$, respectively. Superoxide dismutase (SOD) showed maximum pseudo-activity of 24.78% at ratio of solvent to sample of 22.66 mL/g, ethanol concentration of 45.69%, and extraction temperature of $93.81^{\circ}C$. Based on superimposition of four-dimensional RSM with respect to extraction yield, electron-donating ability, and pseudo-activity of SOD, optimum ranges of extraction conditions were ratio of solvent to sample of 20-30 mL/g, ethanol concentration of 35-65%, and extraction temperature of $50-80^{\circ}C$.

• Journal of the Korean Solar Energy Society
• /
• v.31 no.6
• /
• pp.66-71
• /
• 2011

Various single solvents were tested to find the effective solvent for the extraction of algae oil from wet-form Chlorella minutissima. In the case of single solvents, their extractabilities for algae oil were increased with their polarity because the water in wet algae cell is to form a solvent shell around the lipids. Based on these results, the wet-form algae samples were treated with a polar alcohol solvent and then a nonpolar solvent was added in algae residue. In the algae oil extraction by ethanol/n-hexane, total lipid contents were 40-50% and composition of triglyceride in extracted oil was 46.50%. Considering solvent toxicity of conventional solvent mixture such as chloroform and methanol for algae oil extraction, the ethanol/n-hexane system was identified as the effective one for the oil extraction from wet-form Chlorella minutissima.

• KSBB Journal
• /
• v.15 no.4
• /
• pp.346-351
• /
• 2000

Several solvents or combinations of solvents were tested for the extraction of wet or dried biomass at different extraction mode from plant cell cultures. Methanol gave the highest paclitaxel recovery with the least amount of solvent usage. before extraction drying of biomass wass helpful to decrease solvent usage in extraction step./ in this case drying method was very important to obtain high yield from dried biomass. In thid mode of operation counter-current extraction process can be able to decrease solvent usage but paclitaxel recovery was almost same with both batch and counter-current mode of operation. The number of extraction times was at least four to obtain high yield(>99%) from cell and one to obtain highyield(>96%) from cell debris in batch mode. Equilibrium (i.e. the ratio of paclitaxel in biomass to paclitaxel in the extraction solvent) was reached within 5 minutes. The minimum methodal concentration (90%) and solvent amount(biomass : solvent=1 Kg : 1L) are enough to obtain high yield(>98%) for extraction from biomass.

• Polymer(Korea)
• /
• v.25 no.5
• /
• pp.665-670
• /
• 2001

The solvent extraction of polyethylene glycol (PEG) by ethanol has been studied for the powder injection molded compacts of Sr-ferrite/PEG/carnauba wax/HDPE. The extraction rate of PEG is sensitively proportional to the solvent temperature whereas it is inversely proportional to the contents of PEG, the molecular weight of PEG. and the specimen thickness. The high extraction rate of PEG is achieved under the following conditions: solvent temperature of $70^{\circ}C$, PEG contents of 30%, and PEG molecular weight of 400 g/mol. In the early stage of solvent extraction, the non-linear relationship between the extraction rate and the square root of the extraction time indicates that the extraction of PEG does not follow the Fickian behavior. At extraction times longer than 180 min. however, the extraction of PEG follows the Fickian behavior. The extraction fate of PEG by ethanol is about$1.0{ imes}10^{-6}g/cm^2sec$ enough for common applications.

• Journal of Microbiology and Biotechnology
• /
• v.14 no.5
• /
• pp.932-937
• /
• 2004

In situ extraction by organic solvent was studied in order to improve the recovery yield of hydrocarbon from the culture of Botryococcus braunii, a green colonial microalga. When the solvent mixture of octanol as an extractive solvent and n-octane as a biocompatible solvent was added to a two-phase column, the algal growth was seriously inhibited, even at a low concentration of polar octanol. Therefore, a two-stage cell-recycle extraction process was proposed to improve the contact area between the organic phase and the aqueous phase. The hydrocarbon recovery with in situ cell-recycle extraction showed a three-fold increase (57% of cell) in yield over that with two-phase extraction. In addition, over 60% of the hydrocarbon could be recovered without serious cell damage by downstream separation when this process was applied to the culture broth after batch fermentation.

• Bulletin of the Korean Chemical Society
• /
• v.19 no.12
• /
• pp.1351-1355
• /
• 1998

A simple apparatus for adding a modifying solvent to supercritical CO2 extractant was described. Small, fixed volumes (typically 100 μL) of liquid modifying solvents were delivered during the extraction process by use of an in-line high pressure loop injector and an air pump. Without disconnecting the extraction cell from the supercritical fluid extraction system, the modifying solvent was repeatedly delivered. The solvent modification device was optimized during the extraction of carbaryl and bis(acetylacetonato) copper(Ⅱ). Extraction recoveries from spiked filter paper and soil samples ranged between 22% and 109%, depending on the analyte and matrix components. The addition of polar modifying solvents were necessary to improve the extractability of the nonpolar CO2.