• Bulletin of the Korean Chemical Society
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• v.31 no.8
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• pp.2322-2328
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• 2010

A multiple solid-phase extraction (SPE) method was used with liquid chromatography, coupled with mass spectrometry (LC/MS), for the analysis of heterocyclic amines (HCAs) in human urine. Separation efficiencies based on the pH of the mobile phase and the types of columns were compared. An amide column showed better baseline separation and narrower HCA peak widths at pH 5.0 for the mobile phase than a $C_8$ column. Each SPE step, HLB, MCX, and HybridSPE, was optimized by controlling the pH conditions. The combined method with the three SPEs effectively removed interfering species that cause ion-suppression during HCA detection. Validation of the method, performed with SIM and SRM detection, showed correlation coefficients above 0.991 in the range 0.3 - 16.7 ng/mL. Recovery rates were 45.4 - 97.3% on the $C_8$ column and 71.8 - 101.4% on the amide column, and method detection limits were 0.11 - 0.65 ng/mL on the $C_8$ column and 0.12 - 0.48 ng/mL on the amide column. This method using multiple SPEs offers significant benefits for high-throughput determination of HCAs in urine.

• Steel and Composite Structures
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• v.19 no.2
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• pp.293-308
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• 2015

Interaction between the external thin-walled steel tube and the internal concrete core significantly increases the bending resistance of composite beams and beam-columns in comparison with the steel or concrete members. There is presented a developed method for design of hollow and solid concrete-filled steel tubular beams based on test data, which gives better agreement with test results than EC4 because its limitation to take an increase in strength of concrete caused by confinement contradicts the recommendation of 6.7.2(4) that full composite action up to failure may be assumed between steel and concrete components of the member. Good agreement between the results of carried out experimental, numerical and theoretical investigations allows recommending the proposed method to use in design practice.

• Journal of the Earthquake Engineering Society of Korea
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• v.26 no.2
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• pp.71-82
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• 2022

This paper presents the effect on the inelastic behavior and structural performance of concrete and filled steel pipe through a numerical method for reliable judgment under various load conditions of the CJS composite structural system. Variable values optimized for the CJS synthetic structural system and the effects of multiple variables used for finite element analysis to present analytical modeling were compared and analyzed with experimental results. The Winfrith concrete model was used as a concrete material model that describes the confinement effect well, and the concrete structure was modeled with solid elements. Through geometric analysis of shell and solid elements, rectangular steel pipe columns and steel elements were modeled as shell elements. In addition, the slip behavior of the joint between the concrete column and the rectangular steel pipe was described using the Surface-to-Surface function. After finite element analysis modeling, simulation was performed for cyclic loading after assuming that the lower part of the foundation was a pin in the same way as in the experiment. The analysis model was verified by comparing the calculated analysis results with the experimental results, focusing on initial stiffness, maximum strength, and energy dissipation capability.

• 한국생물공학회:학술대회논문집
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• 2001.11a
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• pp.85-88
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• 2001

We scaled up a covalent immobilization system of urokinase to the activated Sepharose and used it repeatedly to cleave a fusion protein consisting of human growth hormone and GST fragment. After scale up from 6 ml to 250 ml, the column system still demonstrated basically the same performance in terms of urokinase immobilization and fusion protein cleavage. When the column was washed with 6M guanidine HCl after the cleavage reaction. the immobilized urokinase showed no activity probably because it was fully unfolded. However. as the denaturant was gradually removed from the column the immobilized urokinase fully regained its bioactivity. which indicated it was properly refolded into its native conformation as covalently attached to the solid matrix. After 20 cycles of this 'solid-phase unfolding/refolding', the immobilized urokinase maintained approx. 80% of the initial bioactivity. This method provides an efficient protocol to apply the solid-phase refolding technique to improve the longevity of immobilized enzyme columns.

• Steel and Composite Structures
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• v.20 no.1
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• pp.147-166
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• 2016

The paper presents comprehensive quasi-static stability analysis results for a real funnel-flow cylindrical steel silo composed of horizontally corrugated sheets strengthened by vertical thin-walled column profiles. Linear buckling and non-linear analyses with geometric and material non-linearity were carried out with a perfect and an imperfect silo by taking into account axisymmetric and non-axisymmetric loads imposed by a bulk solid following Eurocode 1. Finite element simulations were carried out with 3 different numerical models (single column on the elastic foundation, 3D silo model with the equivalent orthotropic shell and full 3D silo model with shell elements). Initial imperfections in the form of a first eigen-mode for different wall loads and from 'in-situ' measurements with horizontal different amplitudes were taken into account. The results were compared with Eurocode 3. Some recommendations for the silo dimensioning were elaborated.

• Structural Engineering and Mechanics
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• v.77 no.6
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• pp.745-751
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• 2021

Concrete filled double skin tubular members (CFDST) consist of double concentric circular or square steel tubes with concrete filled between the two steel tubes. The CFDST members, having a hollow section inside the internal tube, are generally lighter than ordinary concrete filled steel tubular members (CFT) which have a solid cross-section. Therefore, when the CFDST members are applied to bridge piers, reduction of seismic action can be expected. The present study aims to investigate, experimentally, the behavior of CFDST stub columns with double concentric square steel tubes filled with concrete (SS-CFDST) when working under centric compression. Two test parameters, namely, inner-to-outer width ratio and outer square steel tube's width-to-thickness were selected and outer steel tube's width-to-thickness ratio ranging from 70 to 160 were considered. In the results, shear failure of the concrete fill and local buckling of the double skin tubes having largest inner-to-outer width ratio were observed. A method to predict axial loading capacity of SS-CFDST is also proposed. In addition, the load capacity in the axial direction of stub column test on SS-CFDST is compared with that of double circular CFDST. Finally, the biaxial stress behavior of both steel tubes under plane stress is discussed.

• International Journal of Naval Architecture and Ocean Engineering
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• v.13 no.1
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• pp.718-733
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• 2021

A prediction method, based on the Morison equation as well as Froude-Krylov formula, is presented to simulate the loads acting on the columns and caissons of the semi-submersible platform induced by Internal Solitary Wave (ISW) respectively. Combined with the experimental results, empirical formulas of the drag and inertia coefficients in Morison equation can be determined as a function of the Keulegan-Carpenter (KC) number, Reynolds number (Re) and upper layer depth h₁/h respectively. The experimental and calculated results are compared. And a good agreement is observed, which proves that the present prediction method can be used for analyzing the ISW-forces on the semi-submersible platform. Moreover, the results also demonstrate the layer thickness ratio has a significant effect upon the maximum horizontal forces on the columns and caissons, but both minimum horizontal and vertical forces are scarcely affected. In addition, the incoming wave directions may also contribute greatly to the values of horizontal forces exerted on the caissons, which can be ignored in the vertical force analysis.

• Korean Journal of Environmental Agriculture
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• v.11 no.3
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• pp.201-208
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• 1992

Municipal landfill leachate, a major source of soil contamination and ground water pollution, causes serious environmental problems. To investigate the removal efficiency of pollutants in the leachate by sand, briquet ash, fly ash, and activated carbon columns, COD and some pollutants in the leachate passed through each column for 8 weeks were examined. Average COD removal efficiency for 8 weeks was 83%, 45%, and 43% by activated carbon, briquet ash and fly ash columns, respectively. COD was not effectively reduced by sand column. Average ${NH_4}\;^+$ removal efficiency for 8 weeks was more than 60% by ail columns. Hardness was effectively removed for 8 weeks by fly ash and activated carbon columns. Anoins including $PO_4\;^{3-}$, $CI^-$ and $SO_4^{2-}$ were not removed by all columns.

• Korean Chemical Engineering Research
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• v.47 no.4
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• pp.459-464
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• 2009

Gas-liquid mass transfer characteristics were investigated in pressurized three-phase slurry bubble columns with variation of column diameter. Effects of gas velocity, operating pressure, liquid viscosity, solid content in the slurry phase and column diameter on the gas-liquid volumetric mass transfer coefficient($k_La$) were determined. The effects of operating variables on the mass transfer coefficient tended to change with variation of column diameter. The mass transfer coefficient increased with increasing gas velocity or operating pressure but decreased with increasing column diameter, liquid viscosity or solid concentration in the slurry phase. The increase trend of $k_La$ value with increasing gas velocity and the decrease trend of $k_La$ value with increasing liquid viscosity, tended to decrease gradually with increasing column diameter. However, the effects of operating pressure and solid concentration in the slurry phase on the $k_La$ value did not change considerably with variation of column diameter. The values of $k_La$ were well correlated with operating variables with in this experimental conditions as $k_La=0.02D^{-0.26}U_G^{0.28}P^{0.43}{\mu}_L^{-0.04}S_c^{-0.35}$.

• Journal of the Korean Chemical Society
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• v.34 no.4
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• pp.352-359
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• 1990

An effecient gas chromatographic method is described to be used routinely for the rapid profiling and identification of biochemically important organic acids. It involves the solid-phase extraction of organic acids from aqueous samples using Chromosorb P as the solid sorbent and diethyl ether as the eluting solvent, with subsequent triethylamine treatment. The resulting triethylammonium salts of acids were directly converted to volatile tert-butyldimethylsiyl derivatives, which were simultaneously analyzed by two capillary columns of different polarity, DB-5 and DB-1701, under the identical temperature programming condition. The retention index (RI) and area ratio (AR) values of each peak measured on DB-5 and DB-1701 enabled rapid identification of acids by computer RI library search and AR comparison. The application of the present method to the organic acid profiling of various complex samples is demonstrated.