• 제목/요약/키워드: Sole type

검색결과 153건 처리시간 0.027초

글루탐산을 유일한 탄소원과 질소원으로 이용하는 Acinetobacter sp. B-W의 시드로포어 생산 (Production of siderophore from L-glutamic acid as both carbon and nitrogen sole sources in Acinetobacter sp. B-W)

  • 김경자;장주호;양용준
    • 미생물학회지
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    • 제53권2호
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    • pp.97-102
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    • 2017
  • 포도당과 글루탐산을 함유한 배지에서 시드로포어인 2, 3-dihydroxybenzoic acid (DHB)를 생산하는 Acinetobacter sp. B-W 균주를 글루탐산을 유일한 탄소원과 질소원으로 함유한 배지에 배양한 결과, 상등액에서 2, 3-DHB가 아닌 카테콜 형의 시드로포어를 생산하는 것으로 조사되었다. 글루탐산의 농도는 3%에서 시드로포어 생산이 최고로 나타났으며, 3% 보다 높은 농도에서는 감소하는 것으로 조사되었다. 글루탐산을 유일한 탄소원과 질소원으로 함유한 배지에서 자란 균주 B-W는 배양 온도 $28^{\circ}C$에서는 시드로포어를 생산하지만 $36^{\circ}C$에서는 생산하지 않았다. 또한 $10{\mu}M\;FeCl_3$를첨가한 배지에서는 시드로포어 생산이 완전히 억제되었다. 글루탐산 배지에서 생산된 균주 B-W의 시드로포어는 TLC 전개 용매 butanol: acetic acid: water =12:3:5에서 Rf치가 0.32로 나타나 Rf치가 0.82인 2, 3-DHB와는 다른 것으로 조사되었으며, 또한 항산화 활성도 없는 것으로 나타나 항산화 활성을 가진 2, 3-DHB와는 다른 시드로포어인 것으로 추정되었다.

단조용 니켈기지 초내열합금의 조직예측기술 (Microstructure Prediction Technology of Ni-Base Superalloy)

  • 염종택;김정한;홍재근;박노광
    • 한국소성가공학회:학술대회논문집
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    • 한국소성가공학회 2009년도 추계학술대회 논문집
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    • pp.89-92
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    • 2009
  • As a class of materials, Ni-base superalloys are among the most difficult metal alloys to forge together with refractory metals and cobalt-base superalloys. The mechanical properties of Ni-base superalloys depend very much on grain size and the strengthening phases, $\gamma$' ($Ni_3$(Al,Ti)-type) and $\gamma$".($Ni_3$Nb-type). Especially, the control of grain size remains as a sole means for the control of mechanical properties. The grain size and distribution changes of the wrought superalloys during hot working and heat treatment are mainly controlled by the recrystallization and grain growth behaviors. In this presentation, prediction technology of grain size through the computer-aided process design, and numerical modeling for predicting the microstructure evolution of Ni-base superalloy during hot working were introduced. Also, some case studies were dealt with actual forming processes of Ni-base superalloys.

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Shade Avoidance and the Regulation of Leaf Inclination in Rice

  • Shin, Juhee;Park, Phun Bum
    • Rapid Communication in Photoscience
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    • 제3권3호
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    • pp.53-55
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    • 2014
  • The shade avoidance syndrome is a morphological and physiological response when plants are exposed to shade. Recent work in Arabidopsis had begun to define the molecular components of the shade avoidance syndrome in dicotyledonous model plant. However, little is known about the shade avoidance response networks in agriculturally important monocotyledon crops such as rice. Here, we found that the degree of bending at the lamina joint is inversely proportional to the R:FR ratio. To elucidate which phytochrome is involved in this response, we did lamina joint inclination assay with the rice phytochrome-deficient mutants (osphyA, osphyB, and osphyC) and the wild type plants. Whereas the osphyA and osphyC knockout mutants bent at the lamina joint in the far-red rich condition as the wild type plants, the osphyB knockout mutants no longer bent at the lamina joint in the far-red rich condition. These results suggest that PHYB acts as a sole photoreceptor in the lamina joint inclination response in rice.

Selection of Nitrate-nonutilizing Mutants of Hypoxylon atropunctatum, A Fungal Pathogen on Oak Species

  • Chun, Se-Chul;Fenn, P.
    • The Plant Pathology Journal
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    • 제16권5호
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    • pp.247-251
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    • 2000
  • Latent infections of healthy-appearing oaks of Hypoxylon atropunctatum complicates field studies by interfering with inoculation experiments to follow pathogenesis, fungal development and reproduction of this canker rot fungus. Mutants with unique and easily scorable phenotypes would be useful for inoculation studies. There is a broad range in the capacity of wild-type isolates to utilize nitrate as a sole nitrogen sources. Several types of nitrate-nonutilization mutants (nit1, Nit3, NitM) were selected from nitrate-utilizing wild-type isolates. Also, a few mutants of Hypoxylon atropunctatum were selected that could only grow poorly on basal medium supplemented with various nitrogen sources and even on yeast extract agar. These unknown mutants need to be characterized further. Nit mutants of Hypoxylon atropunctatum were readily selected, grew well and were recovered after inoculation into oak stems. These results suggest that nit mutants could be useful for inoculation studies in trees that contain latent infections.

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The Investigation of Rheological Properties Development for Polymer Matrix Including Foaming Agent

  • Lee, Seung Hak;Kim, Dong Gun;Lim, Sung Wook;Park, Eun Young;Park, Tae Sun;Hyun, Kyu
    • Elastomers and Composites
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    • 제51권1호
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    • pp.24-30
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    • 2016
  • Sole in the footwear usually modified with foaming agent on the polymer resin to improve the lightweightness and crush-cushion effect. In this study, we investigated rheological properties for polymer resin filled with the different type and concentration of foaming agent, capsule type foaming agent and organo-chemical foaming agent, under the time sweep test. Curing times of each polymer resin with different kind of foaming agent are delayed than reference material (epoxy resin with curing agent). In case of adding capsule type foaming agent, however, there is appropriate concentration to reduce the curing time, relatively. When foaming agent is activated, foaming force inflates the sample in contrast to condensation force of curing and then axial normal force develop to the (+) direction. Interestingly, by increase concentration of foaming agent, there is a specific point to break down the axial normal force development. The reason for this phenomenon is that coalescence of foams induce the blocking of axial normal force development.

Selective Isolation and Characterization of Schwanniomyces castellii Mutants with Increased Production of a-Amylase and Glucoamylase

  • Ryu, Yeon-Woo
    • Journal of Microbiology and Biotechnology
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    • 제3권2호
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    • pp.95-98
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    • 1993
  • This study was carried out to isolate and characterize the mutant strains of Schwanniomyces castellii NRRL Y-2477. Mutants were prepared with the treatment of ethyl methane sulfonate. 2-deoxy-D-glucose resistant mutants were isolated and two mutants were selected based on their high production of amylolytic enzymes and their ability to ferment starch. The mutants selected had higher a-amylase and glucoamylase activities than the wild type strain from several other carbon sources. Especially, it was revealed that mutant strain M-9, when cultured in the presence of glucose as a sole carbon source, shows relatively high activities of a-amylase and glucoamylase compared to those of the wild type strain. In result, this mutant strain can be considered as a constitutive producer of amylolytic enzymes. To compare the ethanol production ability of wild type strain and of mutant strains selected, an alcohol fermentation was carried out using 100 g/l soluble starch. Mutant strain M-9 did not improve the direct alcohol fermentation of starch, despite its excellent amylolytic activities performance. On the other hand, mutant strain M-6 produced 37.9 g/l (4.8%, v/v) ethanol by utilizing about 82% of substrate.

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활성슬러지에서 단리한 Acinetobacter sp.에 의한 원유탄화수소분해 (Biodegradation of crude oil hydrocarbons by Acinetobacter sp. isolated from activated sludge)

  • Dong-Hyuk CHOI;Dong Hoon LEE
    • 한국토양환경학회지
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    • 제5권1호
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    • pp.97-108
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    • 2000
  • 원유를 탄소원으로 이용할수 있는 그람 음성 박테리아가 도시하수 처리장의 활성슬러지로부터 단리되어 Acinetobacter속으로 동정되었다. 이 균은 원유중의 알칸과 미확인된 다종의 한화수소를 분해할 수 있었으며 소수성 기질인 알칸을 단일 탄소원으로 이용하여 증식할수 있었다. 원유중 탄소수 13-30의 알칸은 동시에 분해가 진행되었으며 한소수의 짝수, 홀수에 따른 분해특성의 차이는 보이지 않았다. 균의 선형적인 증식과 알칸 성분의 분해특성으로부터, 원유상에서 기질의 초기 산화가 진행되는 미생물부위까지 물질전달이 분해과정의 율속이 됨을 알수 있었다. 분해 진행후 반응계내 알칸의 잔류 현상이 관찰되었는데 이는 Acinetobacter 속의 특성으로 알려져있는 세포내 탄화수소 함유체의 영향으로서 원유로 부터의 물질전달을 제어하는 요인이 될수 있음을 시사하였다. 따라서 본 균은 환경중 잔류하는 소수성 오염물질의 분해메카니즘 연구에 중요한 역할을 한다고 사료된다.

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농작업화에 관한 연구 - 신발종류에 따른 열적 부담 비교연구 - (A study of Agricultural fatigue shoes - A comparative study of heat load by shoe type -)

  • 이경숙;최정화
    • 한국농촌생활과학회지
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    • 제7권2호
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    • pp.99-108
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    • 1996
  • This study has intended to suggest fundamental data to develope and choose appropriate shoes for upland farming in order to prevent health deterioration of women workers and improve work effectiveness and reduce fatigue by wearing appropriate shoes. During 1995. 4. 28 - 5. 10, Fifty women workers in hot pepper farming were observed and major shoe types, which were rubber shoes, walking shoes, slippers, and rubber boots, were selected for the study. During 1995. 10. 9 - 31, two subjects were tested by wearing those shoes in the laboratory where the temperature was 24$\pm$1$^{\circ}C$ and relative humidity 50$\pm$5%RH. And the temperature & humidity on sole and in the shoes, the rectal temperature, skin temperature, blood pressure, pulse, lactate concentration of blood, Flickers' value and subjective sensation were measured. The results were as follows : 1. 84% of women workers mentioned that they need shoes improvement and the order of most frequent shoe types to be worn was rubber shoes, walking shoes, slippers, rubber boots. 2. The rate of women who were unsatisfied with shoes for upland farming is 38 percentages. The reason of unsatisfaction was that feet were in a sweat and alien substances were let into shoes. 3. The temperature & humidity on sole were the lowest in rubber boots during experiment(p<0.01). 4. The relative humidity in the shoes was the highest in rubber boots by 90% and the lowest in walking shoes by 72% during rest And the humidity in slippers and walking shoes were significantly low in experiment(p<0.001). 5. Rubber boots showed the highest rise in rectal temperature by 0.2$^{\circ}C$ showing increase of core temperature (p<0.05). 6. The mean skin temperature during experiment was highest in rubber boots by 33.8$^{\circ}C$(p<0.001).

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외부챔버와 유연한 튜브로 연결된 LCD 패널 검사기 방진용 공기 스프링의 열 및 동적 연성거동에 대한 연구: PART I, 이론적 모델링 (Study on the Thermal and Dynamic Behaviors of Air Spring for vibration isolation of LCD panel inspecting machine connected with an External Chamber through a flexible tube: PART I, Theoretical Modeling)

  • 석종원;이주홍;김필기
    • 반도체디스플레이기술학회지
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    • 제10권1호
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    • pp.33-41
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    • 2011
  • Due to the recent quantum leaps forward in bio-, nano-, and information-technologies (BT, NT and IT), the precisionization and miniaturization of mechanical and electrical components are in high demand. In particular, the ITrelated equipments that take a great part in our domestic industry are in the area requiring high precision technologies. As a consequence, the researches on the development vibration isolation systems that diminish external disturbance or internal vibration are highly required. Among the components comprising the vibration isolation system, air spring has become on a focal point for the researchers due to its merits. This air spring is able to support heavy loads, keep a low natural frequency despite of having a lower value of stiffness, and control the performance of vibration isolation. However, sometimes the sole use of air spring is in demand due to some economic reasons. Under this circumstance, the damping effect of sole air spring may not enough to reduce sufficient amount of vibration. In this study, the air spring mount system connecting with an external chamber is proposed to increase or control the damping effect. To investigate its damping mechanism, the thermal and dynamic behaviors of the system is examined through a theoretical modeling approach in this part of research. In this approach, thermomechanical and Helmholtz resonator type models are to be employed for the air spring/external chambers and connecting tube system, respectively. The frequency response functions (FRFs) derived from the modeling effort are evaluated with physical parametric values and the effects of connecting tube length on these FRFs are identified through computer simulations.

도시주거단지에 나타난 색채사용특성에 관한 연구 - 독일 포츠담시 Kirchsteigfeld 주거단지를 중심으로 - (A study on the characteristics of color application indicated from urban housing complex - A Forcuse on the Kirchsteigfeld Housing Complex in Potsdam City -)

  • 이선민;이민석
    • 한국실내디자인학회논문집
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    • 제16권6호
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    • pp.250-257
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    • 2007
  • Characteristics of color application indicated from Kirchsteigfeld is presented the various points of view to be preserved urban housing color. It had been handled as environmental color by view point of community and connectivity rather than color scheme of single housing. First, color to be consisted of basis for urban entirely as background color in urban housing complex has been indicated the bright and cheerful feelings and expressed to be introduced mostly from the single color system. Second, characteristics to be inhibited strongly from housing complex had been indicated by restricted four types of color, formulated the overall context and change through accent and the change from disposition of principal and the accessary of color scheme. In this case, it was not regular formulation, but as a whole category view, it could be provided by orderly regularity system strictly Particularly it had been applied as a whole for lightness change by single color, introduced the different color of building to be centered or remained by a visual point. This had been formed to introduction of color by stand points of housing group not introduction to color by sole concept of building. Third, emphasizing of morphologic feeling by using of difference of lightness, it could be indicated the mild and comfortable stereo-type sensibility from the overall types of housing, we could understand for formation of color code by lightness of slightly differences. Fourth, it had been taken the convenient adaptation method for maintenance of simple graphic system as a environmental color. It has considerable advantage for sustainability of color to indicate on finishing material, form and detail in architecture, as the sole separated color not to be complicated when to be painted over and again or managed continuously.