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Biodegradation of trichloroacetic acid from organic solvent tolerant bacterium, Pseudomonas savastanoi BCNU 106

  • 김종수;박형철;조수동;이승한;김기욱;문자영;정영기;주우홍
    • 한국생물공학회:학술대회논문집
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    • 한국생물공학회 2003년도 생물공학의 동향(XII)
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    • pp.390-392
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    • 2003
  • Pseudomonas savastanoi BCNU 106은 mineral salt medium에서 30 mM trichloroacetic acid의 농도까지 유일한 탄소원으로 이용하여 생육할 수 있다는 것을 발견하였다. 또한 trichloroacetic acid 뿐만 아니라 monochloroacetic acid, trichloroethylene, p-dichlorobenzene을 유일한 탄소원으로 이용 가능하였다. 그러나 이들 화합물에 대한 내성시험결과 전혀 내성이 없는 것을 알게 되었다.

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Benzene, toluene, ethylbenzene 그리고 세가지 xylene isomer를 분해하는 유기용매 내성세균 Pseudomonas savastanoi BCNU 106의 분리 및 분해 특성

  • 김종수;박형철;조수동;이승한;배윤위;문자영;정영기;주우홍
    • 한국생물공학회:학술대회논문집
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    • 한국생물공학회 2003년도 생물공학의 동향(XII)
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    • pp.382-385
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    • 2003
  • Organic solvent tolerant bacterium, designated as strain BCNU 106 is a gram negative, rod-shaped aerobe and grows on benzene, toluene, ethylbenzene, and xylenes (BTEX) as a sole carbon source. According to 16S rDNA analysis and fatty acid analysis, strain BCNU 106 showed highest similarity to Pseudomonas syringae var. savastanoi (Pseudomonas savastanoi). Strain BCNU 106 was able to utilize toluene, ethylbenzene, both o-, m-, p-xylene , m-cresol and o-cresol. The degradation of o-, m-, p-xylene by strain BCNU 106 is particularly important, since o-xylene is a compound of considerable environmental interest, owing to its recalcitrance; and very few microorganism have been reported to utilize both o-, m-, p-xylene as a sole carbon source.

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해양에서 분리된 Klebsiella sp. KCL-1에 의한 원유분해 특성의 규명 (Characterization of Crude Oil Degradation by Klebsiella sp. KCL-1 Isolated from Sea Water)

  • 차재영;정선용;조용수;최용락;김범규;이영춘
    • 한국미생물·생명공학회지
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    • 제27권6호
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    • pp.452-457
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    • 1999
  • Several bacterial strains utilizing crude oil as their sole carbon and energy sources were isolated from marine. One of the strains named KCL-1 showed the highest degradative activity for crude oil and the best growth rate. This strain was identified as a Klebsiella sp. based on the morphological, biochemical, and physiological characteristics. The optimum cultural conditions were as follows; $32^{\circ}C$~$37^{\circ}C$ for temperature and 7.0 for initial pH. Additionally, the optimal concentration of sodium chloride was 3.0%, indicating that this strain was derived from seawater. KCL-1 could use several kinds of n-alkane hydrocarbons from octadecane to hexacosane as a sole carbon source. The degradation of crude oil by KCL-1 was stimulated by addition of octadecane in the culture. The emulsifying activity by KCL-1 was highest after 3 days of cultivation under the condition of 3.0% sodium chloride, pH 7.0 and $37^{\circ}C$.

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마사이 기린에서 발의 불완전 결출당 치유 1례 (Management of Incomplete Avulsion Injury of the Foot in a Masai Giraffe (Giraffe camelopardalis tippelskirchi))

  • 권수완;황범태;정순옥;권오경
    • 한국임상수의학회지
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    • 제14권1호
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    • pp.118-122
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    • 1997
  • The lateral hoof of right hind limb of a pregnant Masai giraffe was accidentally injured, and its coronet, hoof wall, and sole were detached in a mass. The bleeding was observed from the exposed dermis of the affected foot for several hours, with second scoreof supporting lameness. The bleeding from the digital artery at the exposed dermis was stopped by epinephrine spray and direct pressing. Nitrofurazone ointment was topocally applied for 5 days and sxytetracycline solusion was delivered by a fluid jet in the affected hoof one month. The calf was born 5 months after the avulsion. The horn of the hoof wall was grown from the coronet to sole, and was gradually filled from the heel to the toe. The growth rate and filling rate of the hoof wall were 0.32mm and 0.54mm a day, respectively.

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Cyclohexanol 이용성 세균의 분리 및 특성 (Isolation and Characterization of Cyclohexanol-utilizing Bacteria)

  • 김태강;이인구
    • 한국미생물·생명공학회지
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    • 제27권2호
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    • pp.107-112
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    • 1999
  • A bacterium, which can utilize cyclohexanol as a sole source of carbon and energy, was isolated from sludge in sewage of Ulsan Industrial Complex for Petrochemicals, Korea and identified as Rhodococcus sp. TK6. The growth conditions of the bacteria were investigated in cyclohexanol containing media. The bacteria utilized cyclohexanol, cyclohexanone, cyclohexane-1,2=diol, cyclopentanol, cyclopentanone, and $\varepsilon$-caprolactone but not cyclohexane, cyclohexane-1,2-dione, and cyclooctanone. The bacteria were able to utilize alcohols such as ethanol, 1-propanol, 1-butanol, 1-pentanol, 1-hexanol, 2-methyl-1-propanol, 3-methyl-1-butanol, 2-propanol, and 2-butanol as well as cyclohexanol, organic acids such as adipate, propionate, butyrate, valerate, n-caproate, and 6-hydroxycaproate, and aromatic compounds such as phenol, salicylate, p-hydroxbenzoate, and benzoate as a sole source of carbon and energy. Cyclohexanone as a degradation product of cyclohexanol by Rhodococcus sp. TK6 was determined with gas chromatography.

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호기적 Trichloroethylene 공동대사 세균의 분리 및 특성 (Isolation and Characterization of Aerobic Trichloroethylene Cometabolizing Bacterium)

  • 김호성;박근태;손홍주;박성훈;이상준
    • 한국환경과학회지
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    • 제10권2호
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    • pp.99-103
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    • 2001
  • Several microorganisms which degrade phenol and trichloroethylene(TCE) were isolated from the activated sludge of a wastewater treatment plant. Among them, one isolate EL-04J showed the highest degradability and was identified as a Pseudomonas species according to morphological, cultural and biochemical properties. The phenol-induced cells of Pseudomonas EL-04J, which were preincubated in the mineral salts medium containing phenol as a sole carbon source, degraded 90% of 25$\mu$M TCE within 20h. This strain could also utilize some of methylated phenol derivatives (o-cresol, m-cresol and p-cresol) as the sole source of carbon and energy. Cresol-induced cells of Pseudomonas EL-04J also cometabolized TCE.

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이족 로봇을 위한 동적 보행 해석 (Dynamic Walking Analysis for Biped Robot)

  • 박인규;김진걸
    • 대한전기학회:학술대회논문집
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    • 대한전기학회 2000년도 하계학술대회 논문집 D
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    • pp.2804-2807
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    • 2000
  • This paper suggests a method of the forward dynamic analysis for the computer simulation on the analysis of the dynamic behavior for biped walking robot. The equations f motion of the system or the simulation are constructed by using the Method of the multibody dynamics which is powerful method for modeling of the complex biped system. For the simplicity of simulation, we consider that the sole of the contacting foot is affected by the reaction forces for tree structure system topology instead of the addition or deletion of the kinematic constraints. The ground reaction forces can be modeled using the simple spring and damper model at the three contacting points on the sole of the foot. For minimizing the errors of numerical integration, the number of equations of motion is minimized by adding the driving constraints or a controller instead of the direct driving torques.

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Stable Isotope Labeled Cytochrome $c_3$ from Desulfovibrio vulgaris on a Defined Medium as Sole Nitrogen Source

  • Kim, Andre;Shim, Yoon-Bo;Kang, Shin-Won;Park, Jang-Su
    • BMB Reports
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    • 제33권6호
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    • pp.506-509
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    • 2000
  • To obtain Cytochrome $c_3$ labeled with a stable isotope, the conditions of cultivation and the composition of medium for DvMF were examined. The growth of DvMF was steady and reproducible under purging with $N_2$ and under pH control. DvMF was able to go on a defined medium without natural products. The composition of the medium containing a small amount of $NH_4Cl$ as sole nitrogen source was established. Then, uniformly $^{15}N-labeled$ Cytochrome $c_3$ was obtained during the culture of DvMF in a defined medium with $^{15}NH_4Cl$; it was confirmed by $^{1}H-^{15}N$ HMQC.

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개산형 방산계약에서의 원가이전에 관한 실증연구 (Study on Cost-shifting in Cost Reimbursement type of Defense Contracts)

  • 이정동;이춘주;정경인
    • 한국국방경영분석학회지
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    • 제31권2호
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    • pp.1-12
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    • 2005
  • Defense firms' excess profitability by shifting common overhead costs from non-defense sector to defense sector in the award of cost reimbursement type of defense contracts is a widespread conclusion in prior researches. In this paper, we reviewed cost-shifting incentives in defense-contracting firms and extended the analysis of McGowan and Vendrzyk(2002) by applying modified model to Korea Defense Contracts. We formulated six hypotheses to test the relation of cost-shifting and excess profitability regarding market openness, auditing system, ranking in defense contracts, and firm types(sole defense contractors, sole non-defense contractors, and defense and non-defense mixed contractors). But, we could not find any evidence that defense firms shifted costs from non-defense sector to defense sector for the period 1997-2002. The results suggest that the excess profitability of Korea defense firms may not be from the cost-shifting but from other reasons.

Degradation of Phenanthrene by Sphingomonas sp. 1-21 Isolated from Oil-Contaminated Soil

  • Ryeom, Tai-Kyung;Lee, Il-Gyu;Son, Seung-Yeol;Ahn, Tae-Young
    • Journal of Microbiology and Biotechnology
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    • 제10권5호
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    • pp.724-727
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    • 2000
  • A Phenanthrene-degrading bacterium, Strain 1-21 was isolated from oil-contaminated soil. This strain was a Gram-negative, aerobic, and rod-shaped bacterium, and exhibited a 99% sequence similarity of 16S rDNA to that of Sphingomonas subarctica. The major cellular fatty acid was a summed feature 7(18:1 w7c, 18:1 w9t, 18:1 s12t), which is a characteristic of the Sphingomonas species. When 200 and 1,000 ppm of phenanthrene was added as the sole carbon source, Strain 1-21 degraded 98% and 67% after 10 days of incubation, respectively. Futhermore, this strain was also able to utilized naphthalene and fluorene as sole carbon and energy sources.

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