• BMB Reports
• /
• 제34권2호
• /
• pp.172-175
• /
• 2001

Catechol 1,2-dioxygenase $I_1$ ($CDI_1$) is the first enzyme of the $\beta$-ketoadipate pathway in Acinetobacter lowffii K24. $CDI_1$ has two cysteines (155, 202) and its enzyme activity is inhibited by the cysteine inhibitor, $AgNO_3$. Two mutants, $CDI_1$ C155V and $CDI_1$ C202V, were obtained by site-directed mutagenesis. The two mutants were overexpressed and the mutated amino acid residues (Cys$\rightarrow$Val) were characterized by peptide mapping and amino acid sequencing. Interestingly, $CDI_1$ C155V was inhibited by $AgNO_3$, whereas $CDI_1$ C202V was not inhibited. This suggests that $Cys^{202}$ is the sole inhibition site by $AgNO_3$ and is close to the active site of the enzyme. However, the results of the biochemical assay of mutated $CDI_1s$ suggest that the two cysteines are not directly involved in the activity of the catechol 1,2-dioxygenase of $CDI_1$.

• 농촌지도와개발
• /
• 제8권2호
• /
• pp.209-221
• /
• 2001

The nature of work which is changing has increased the importance of HRD within organization. Also, these changes are making HRD shift its paradigm : from the segregated, closed, operational & administrative, dependent, utilitarian view to the integrative, open, strategic & facilitative, interdependent, generative view. The shifts of the perspective on HRD cause HRD professionals rethink the purpose and definition of HRD, and the role & competency needed to HRD in 21st century. The findings which are drawn through literature review and researcher’s intuition based on a long field experience are as follows. First, The function of HRD has recently been much more important then that of the past. Second, There is no consensus over the conceptual identity of HRD. Nonetheless, it is apparent that the preferred activities of the HRD function is performance improvement. Third, It is too difficult that we should define clearly the role & competency of HRD because of diverse perspectives on them. But we find much evidence converging to support the fact that the role & competency of HRD should be extended from the sole focus on training & development to adding the additional role & competency of change agent, who includes planning, implementing, and managing change.

• 패션비즈니스
• /
• 제13권5호
• /
• pp.121-134
• /
• 2009

The purpose of this study was to analyze the wearing pattern and design preference of shoes for men, and to develop the possibility and strategy of the shoes market for the shoes marketers and manufacturers. In this study, the data obtained from 285 respondents were analyzed by the descriptive statistics. The results from the data were as follows : The most frequent brand among the 45 shoes brand by 285 respondents described in free style was 'Esquire'. The 268 respondents possessed two shoes or more, the most frequent shoes' color was black, and the most preferred brand was 'Kumkang'. The 195 respondents indicated the discomfort of ready-made shoes, The 198 respondents discarded their shoes 'on the reason of worn-out', the 98 respondents indicated that the most important thing in the purchasing point was 'the comfort of shoes' The respondents preferred shoes with 'slip-on type', 'cow leather', 'semi-rounded toe', 'no-metal ornaments', 'moccasin tip', 'leather-sole', and '3cm heel'. Finally, this study proposed that the best strategy for shoes marketers and manufacturers was to upgrade the comfort of shoes by design(line) and the material with functional textures.

• 한국식품영양과학회지
• /
• 제11권2호
• /
• pp.31-35
• /
• 1982

폐액으로 방류되는 두부 폐수(whey)를 이용하여 alcohol 발효를 실시하였으며 다음과 같은 결과를 얻었다. whey중(中)에 함유(含有)된 환원당은 약 0.71%로서 이당(糖)을 이용(利用)하며 l당(當) 최대(最大) 1.86 g의 ethanol이 생산(生産)되었으나 수률(收率)이 낮아서 경제성은 희박하다. 그러나 발효에 의해서 단백질과 당이 각각 94%, 81%만큼이나 감소한 결과(結果)로 미루어 보아 두부 폐수중(廢水中)의 유기물(有機物)을 alcohol 발효(醱酵)에 이용(利用)할 수 있음을 확인(確認)하였다.

• 미생물학회지
• /
• 제31권2호
• /
• pp.99-104
• /
• 1993

Two strains of the gram-negative acetic acid bacteria, Acetobacter sp. strain CS2- AND CS5, were isolated form the traditional raw rice wine vinegar of Haenam area. The strains oxidized ethanol to acetic acid and over-oxidized acetate and lactate to $CO^{2}$ and $H ^{2}$O. They produced 2-ketogluconic acid from glucose but did not produce .gamma.-pyrones from glucose and dihydroxyacetone from glycerol. The CS strains possessed ubiquinone-9 as a major isoprenoid quinone and contained straight-chain $C_{18 :1}$, $_C{16 : 0}$, and $C _{14 : 0}$ fatty acids. The DNA base composition of the CS2 and CS5 strains was 56.2 and 57.3 mole% G + C, respectively. The isolates were grown well on methanol, gluconate, erythritol, raffinose, dulcitol and xylitol as sole sources of carbon and energy which are different from those of other Acetobacter species and producedd acid from sucrose, glycerol, fructose, inositol, mannitol, and ribose.

• 한국환경과학회지
• /
• 제10권5호
• /
• pp.359-364
• /
• 2001

Pseudomanas sp. EL-04J was previously isolated from phenol-acclimated activated sludge. This bacterium was capable of degrading phenol and cometabolizing trichloroethylene (TCE). After precultivation in the mineral salts medium containing phenol as a sole carbon source, Pseudomonas EL-04J degraded 90% of TCE $25 \mu\textrm{M}$ within 20 hours. Thus, phenol-induced Pseudomonas sp. EL-04J cells can bdegrade TCE. Followsing a transient lag period, Pseudomonas sp. EL-04J cells degraded TCE at concentrations of at least $250 \mu\textrm{M}$ with no apparent retardation in rate, but the transformance capacity of such cells was limited and depended on the cell concentration. The degradation rate of TCE followed the Michaelis-Menten kinetic model. The maximum degradation ratio ($V_{max}$) and saturation constant ($K_{m}$) were $7nmo {\ell}/min{\cdot}mg$ cell protein and $11 \mu\textrm{M}$, respectively. Cometabolism of TCE by phenol fed experiment was evaluated in $50m {\ell}$ serum vial that contained $10m {\ell}$ of meneral sals medium supplemented with $10 \mu\textrm{M}$ TCE degradation was inhibited in the initial period of 1 mM phenol addition, but after that time Pseudomonas sp. EL-04J cells degraded TCE and showed cell growth.

• Nutrition Research and Practice
• /
• 제10권2호
• /
• pp.237-244
• /
• 2016

BACKGROUND/OBJECTIVES: The current South Korean government policy on food irradiation technology should be reformed based on an in-depth investigation of the communications aspect, because the issue is no longer of a technological nature, given the proven safety and efficacy of the processes. SUBJECTS/METHODS: The target population of the education program consisted of elementary, middle, and high school students attending 310 schools in South Korea (2013: 63 schools, 2014: 104 schools, 2015: 143 schools). Data subjected to analysis were 13,327 pre-education and 12,641 post-education questionnaires received from 7,582 elementary, 2,671 middle, and 3,249 high school students who participated in the education program from May 2012 to April 2015 (n = 12,831), after the exclusion of inadequately filled-in questionnaires. RESULTS: Analysis of the three-year educational effect trend was conducted by comparing levels of variables before and after food radiation education. The analysis yielded the finding that the post-education levels were significantly higher for all variables. That is, for interest in education, perception (necessity, safety, subjective knowledge, and information acquisition), objective knowledge, and attitude, with the sole exception of objective knowledge in 2013. CONCLUSIONS: Given that post-education levels of perception, knowledge, and attitude concerning irradiated foods increased considerably compared to pre-education levels, behavior change should be induced by providing continuous education to enhance, these primary variables.

• 한국생활과학회지
• /
• 제23권2호
• /
• pp.205-217
• /
• 2014

This study examined attitudes of grandmothers who had experience in grandchild care in order to explore their subjectivity. Research questions were 1) to categorize the attitudes toward grandchild care, and 2) to analyze each type's characteristics and elucidate the analyses. The results are as follows. Grandmothers' attitudes toward grandchild care were categorized into three types: (1) adjusting-to-role-change type, (2) recognizing-the-role-burdens type, (3) enjoying-the-traditional-role type. Type 1, the adjusting-to-role-change type was aware of positive facets of grandchild care, and actively accepted the caring responsibility, but did not think it was a grandmother's duty to care for the grandchildren or that it was the sole pleasure in a grandmother's life. Type 2, the recognizing-the-role-burdens type, had a negative view regarding grandchild care, perceiving the cost too high relative to the reward. On the other hand, Type 3, the enjoying-the-traditional-role type considered grandchild care as a pleasure and a duty, and downplayed negative aspects such as having no time to herself, assuming the traditional grandmother role and enjoying it. These results imply that the attitudes of grandmothers nowadays are undergoing a change from the traditional grandmother role attitude.

• 한국안광학회지
• /
• 제12권3호
• /
• pp.137-142
• /
• 2007

이 연구는 굴절성 약시에서 가림 치료를 대신할 수 있는 방법들 중 안경 교정에 의한 약시치료효과와, 부등굴절력과 난시와의 관계를 연구하였다. 안경교정치료는 기존의 가림 치료 효과를 주도록 정상안에 대해 저교정을 하여 차폐 효과를 주는 것으로 원시성약시를 교정하는 추가적 처방 없이 안경 착용만으로 치료하였다. 안경교정에 의한 약시치료 결과 원시성약시에서 부등도수와의 관계 연구에서 다음과 같은 결과를 얻었다. 1. 양안의 부등굴절력이 클수록 치료 전 약시안의 교정시력이 낮았다. 2. 부등굴절력이 치료 후 교정시력에 영향을 주지 않았다. 3. 치료시작 나이가 늦을수록 난시굴절력이 높게 나타났다. 4. 차폐 없이 안경교정으로 약시치료의 효과를 얻을 수 있었다.

• Journal of Microbiology and Biotechnology
• /
• 제8권2호
• /
• pp.134-140
• /
• 1998

Eubacterium limosum KIST612 was cultured on phosphate-buffered basal medium (PBBM) with carbon monoxide (CO) as the sole energy and carbon source. The initial growth rate of this strain was approximately 0.17~0.25 $h^-1$/ and the $K_s$ value for dissolved substrate was 0.14 mM. CO was limiting during the growth of the bacterium when the CO partial pressure was less than 0.6 atm (0.5 mM dissolved CO). The bacterial growth rate was reduced in the presence of acetate. When sufficient CO was supplied using a gas-lift reactor, the acetate concentration went up to 90 mM in 116 h. Based on these findings, it is suggested that a pressurized reactor be used to develop a process to convert CO-rich gases into multi-carbon compounds.