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A Simple Java Sequence Alignment Editing Tool for Resolving Complex Repeat Regions

  • Ham, Seong-Il;Lee, Kyung-Eun;Park, Hyun-Seok
    • Genomics & Informatics
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    • v.7 no.1
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    • pp.46-48
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    • 2009
  • Finishing is the most time-consuming step in sequencing, and many genome projects are left unfinished due to complex repeat regions. Here, we have developed BACContigEditor, a prototype shotgun sequence finishing tool. It is essentially an editor that visualizes assemblies of shotgun sequence fragment reads as gapped multiple alignments. The program offers some flexibility that is needed to rapidly resolve complex regions within a working session. The sole purpose of the release is to promote collaborative creation of extensible software for fragment assembly editors, foster collaborative development, and reduce barriers to initial tool development effort. We describe our software architecture and identify current challenges. The program is available under an Open Source license.

Effect of Cathodic Biofilm on the Performance of Air-Cathode Single Chamber Microbial Fuel Cells

  • Ahmed, Jalal;Kim, Sung-Hyun
    • Bulletin of the Korean Chemical Society
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    • v.32 no.10
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    • pp.3726-3729
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    • 2011
  • Biofilm formation is inevitable in a bioelectrochemical system in which microorganisms act as a sole biocatalyst. Cathodic biofilm (CBF) works as a double-edged sword in the performance of the air-cathode microbial fuel cells (MFCs). Proton and oxygen crossover through the CBF are limited by the robust structure of extracellular polymeric substances, composition of available constituents and environmental condition from which the biofilm is formed. The MFC performance in terms of power, current and coulombic efficiency is influenced by the nature and origin of CBF. Development of CBF from different ecological environment while keeping the same anode inoculums, contributes additional charge transfer resistance to the total internal resistance, with increase in coulombic efficiency at the expense of power reduction. This study demonstrates that MFC operation conditions need to be optimized on the choice of initial inoculum medium that leads to the biofilm formation on the air cathode.

Laboratory Experiment: Synthesis and Characterization of 4-Methyl-N-(phenylacetyl)benzenesulfonamide through Cu(I)-Catalysis

  • Jung, Byunghyuck
    • Journal of the Korean Chemical Society
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    • v.62 no.3
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    • pp.187-190
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    • 2018
  • A three-component coupling reaction of phenylacetylene, p-toluenesulfonyl azide, and water through copper catalysis is described to provide knowledge of spectroscopy and catalytic reactions and to introduce current research topics in organic chemistry for second-year undergraduate students. In the presence of stoichiometric amounts of phenylacetylene, p-toluenesulfonyl azide, and triethylamine, the reaction was performed with 4 mol% CuCl in water as the sole solvent and was completed in 1.5 h. A practical purification method and recrystallization of the crude reaction mixture resulted in the rapid isolation of the desired product with yields of 42~65%. Students characterized 4-methyl-N-(phenylacetyl)benzenesulfonamide by using melting-point determination, infrared spectroscopy, and nuclear magnetic resonance (NMR) spectroscopy. This experimental procedure and spectroscopic data analysis will serve as a platform for students to apply classroom knowledge in practical state-of-the-art research.

Vegetation structure and ecological properties of Picea jezoensis community (가문비나무(Picea jezoensis)군락의 식생구조와 생태특성)

  • Do, Mi-Sole;Lee, Ji-Hye;Gwon, Jae-Hawn;Song, Ho-Kyung
    • Korean Journal of Agricultural Science
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    • v.39 no.4
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    • pp.525-534
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    • 2012
  • This study was conducted to classify community and verify vegetation structure of Picea jezoensis community in subalpine zone of Mt. Kyebang, Mt. Jiri, Mt. Deogyu, 39 releves set up and was carried out to analyze ordination. P. jezoensis Community was classified into Acer ukurunduense subcommunity, Quercus mongolica subcommunity, Syringa reticulata var. mandshurica subcommunity and P. jezoensis typical subcommunity. The order of important value of the forest community with DBH 2cm above plants was P. jezoensis (89.22), Acer komarovii (30.50), Abies koreana (18.81), Rhododendron schlippenbachii (18.10), Sorbus commixta (17.19), Betula ermanii (16.89), Pinus koraiensis (16.80), Taxus cuspidata (12.45), Acer ukurunduense (11.73). The DBH analysis suggests that P. jezoensis will maintain dominant position.

Leader-Following Formation Control of Multiple Robots with Uncertainties through Sliding Mode and Nonlinear Disturbance Observer

  • Qian, Dianwei;Tong, Shiwen;Li, Chengdong
    • ETRI Journal
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    • v.38 no.5
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    • pp.1008-1018
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    • 2016
  • This paper presents a control scheme for the leader-following formation of multiple robots. The control scheme combines the sliding mode control (SMC) method with the nonlinear disturbance observer (NDOB) technique. The formation dynamics suffer from uncertainties because the individual robots are uncertain. Concerning such formation uncertainties, the leader-following formation dynamics are modeled. Assuming that the formation uncertainties have an unknown boundary, an NDOB-based observer was designed to estimate the formation uncertainties. A sliding surface containing the observer outputs has been defined. Regarding the sliding surface, an SMC-based controller was investigated to form uncertain robots. A sufficient condition in the sense of the Lyapunov theory was proven such that the formation system is asymptotically stable. Herein, some comparison results between the sole SMC method and the second-order SMC method are presented to demonstrate the effectiveness and feasibility of the control scheme for multiple robots in the presence of uncertainties.

Biodegration of Formaldehyde-Releasing Preservatives

  • Park, Won-Jae;Yang, Seung-Kak
    • Journal of the Society of Cosmetic Scientists of Korea
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    • v.11 no.1
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    • pp.1-12
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    • 1985
  • A strain of Pseudomonas aeruginosa was isolated from the spoiled product and its characteristics on various formaldehyde-releasing preservatives were investigated. This strain, P. aeruginosa FR, could utilize 1.0% of imidazolidinyl urea and 0.2% of DMDM hydantoin as a sole carbon and nitrogen source in the minimal salts medium. With the growth of the strain in minimal salts medium containing imidazolidinyl urea, formic acid was initially accumulated according to the decrease of formaldehyde concentration. It was suggested that formaldehyde dehydrogenase was involved in this oxidation process and could catalyze formaldehyde, imidazolidinyl urea, DMDM hydantoin and quaternium-15, but not bronopol. MICs of this strain to each preservation were 0.03% in formaldehyde, 1.0% in imidazolidinyl urea, 0.2% in DMDM hydantoin, 0.2% in quaternium-15 and 0.1% of EDTA-2Na. But the MICs were diminished about ten times when 0.01% of EDTA-2Na was added to the preservative systems. In actual challenge test, the eyeliner and the pack which contained paraben and imidazolidinyl urea were not able to be protected from this strain, but when 0.05% EDTA-2Na was added the products were sufficiently preserved.

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증발을 고려한 Wafer Spin Coating 박막 예측에 관한 수치 해석적 연구

  • 노영미;임익태;김광선
    • Proceedings of the Korean Society Of Semiconductor Equipment Technology
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    • 2002.11a
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    • pp.20-26
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    • 2002
  • The fluid flow, mass transfer, heat transfer and film thickness variation during the spin coating process are numerically studied. The model is said to be 1-dimensional because radial variations in film thickness, concentration and temperature are ignored. The finite difference method is employed to solve the equations that are simplified using the similarity transformation. In early time film thinning is due to the radial convective outflow. However that slows during the first seconds of spinning so the film thinning due to evaporation of solvent becomes sole. The time various film thickness is analyzed according to the var ious solvent fraction in the coating liquid and in the bulk of the overlying gas and the temperature variation in the liquid film during the spin coating is estimated.

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The utilization of acetate for the growth and the respiration in Dunaliella tertiolecta.―Enzymes of the tricarboxylic acid cycle and glyoxylate pathway (Dunaliella tertiolecta에 의한 acetate의 이용 -TCA cycle과 glyoxylate pathway의 활성 조사-)

  • 권영명
    • Journal of Plant Biology
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    • v.16 no.1_2
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    • pp.6-11
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    • 1973
  • The utilization of acetate by Dunaliella tertiolecta was examined, and the detections and assays of the enzymes of the tricarboxylic acid cycle and the glyoxylate pathway were described. Acetate could not be utilized as a sole carbon source for the growth. The carboxyl carbon of acetate was incorporated more rapidly into CO2 than the methyl carbon. It was identified that malate, succinate, citrate and etc., were accumulated whne [U-14C] acetate was supplied to the cell free homogenate. The following enzyme activities were measured; acetothiokinase, isocitrate dehydrogenase, fumarase, malate dehydrogenase and aconitase. Though isocitratase, malate synthetase, succinate dehydrogenase and oxoglutarate dehydrogenase could not be detected, 14C from succinate was easily contributed to CO2 and cell component. The evidence suggested that the glyoxylate pathway was not operative and showed that the TCA cycle was the all important pathway in the oxidation of acetate to CO2 in Dunaliella.

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Two Species of Copepoda (Poecilostomatoida . Siphonostomatoida) assoicated with Asteroida in Korea (한국산 불가사리에 공생하는 요각류 2 종)

  • 김일회
    • Animal Systematics, Evolution and Diversity
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    • v.8 no.1
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    • pp.57-68
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    • 1992
  • Only four copepod species associated with sea stars have beenknown from the north Pacific . The examination of Korean sea stars for copepod associates revealed two species ; Scottomyzson gibberum and a new species belonging to the genus Synstellicola. Both species are (re) described. S. gibberum , A sole member of the genus hitherto known only from the northeastern Atlantic , is recorded in this report to be associated with various sea stars and distributed widely in Korean seas.

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A Study of Nasalance for Normal Korean Children Using Nasometer II (정상 소아의 비음도에 관한 연구)

  • Kim, Seong-Il;Jo, Sang-Ki;Ko, Seung-O;Shin, Hyo-Keun
    • Speech Sciences
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    • v.7 no.4
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    • pp.73-82
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    • 2000
  • The perceptual judgement of nasality is still used in the assessment of velopharyngeal incompetence, but it should not be the sole criterion for speech nasality. Objective procedures may be used to assess velopharyngeal function, for example, nasometer, aerodynamics, x-ray, electromyography, nasoendoscopy, and videofluoroscopy can be utilized. The nasometer employs noninvasive measurement methods and measures with high levels of accuracy. The aim of this study was to obtain comprehensive nasalance data for Korean children, aged 7 years, and to investigate any gender differences within that age group. The results were as follow: 1. Statistically, gender has no significant effect on the nasalance of vowels /a/, /i/, /o/, /u/, /je/, /wi/) but the nasalance of the vowels /e/, /ja/) was higher in males (p<0.05). 2. There was no statistically significant effect of gender in plosives /p/, /ph/, /p'/, /t/, /th/, /t'/, /k/, /kh/, /k'/), affricatives /c/, /ch/, /c'/), and fricatives /s/, /s'/, /$\int$/). 3. The nasalance of the nasal consonants, /m/, /n/, /an/) is higher in males and only /n/, /an/ were statistically significant (p<0.05).

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