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Sensory Characteristics of Citrus Vinegar fermented by Gluconacetobacter hanenii CV1 (Gluconacetobacter hansenii CV1에 의해 발효된 감귤식초의 관능적 특성)

  • Kim Mi-Lim;Choi Kyung-Ho
    • Korean journal of food and cookery science
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    • v.21 no.2 s.86
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    • pp.263-269
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    • 2005
  • Citrus juice, a concentrate manufactured by the Jeju Provincial Corporation, was converted into vinegar orderly by alcohol and acetate fermentation. The juice with 6 folds dilution by distilled water was used as the sole nutrient source through out experiments. Diluted juice contained $12.96^{\circ}Brix$ of total sugar, $0.632\%$ of total acid and $20.23{\mu}g/m{\ell}$ of hesperidin. Naringin was not detected from the juice. Citrus wine having $5.6\~6.3\%$ alcohol was produced from diluted juice by 3 days of fermentation at $28^{\circ}C$. A kind of malomelo yeast CMY-28 was used for wine fermentation. The wine was succeedingly fermented for 8 days at $30^{\circ}C$ after inoculation of seed vinegar which contained active cells of acid producing bacteria CV1. Inoculum size of seed vinegar was controlled to $10\%$(v/v) of citrus wine. The wine converted into vinegar by the fermentation. Citrus vinegar, the final product of fermentation, was colored with very thin radish-yellow and transparent. It's acidity ranged between $5.8\~6.2\%$ as acetic acid. The vinegar got the best score by sensory test among several natural fruit vinegars. It was clear from the results that citrus vinegar in high quality could be produced from concentrated citrus juice, however fermentation conditions should be improved to reduce the amount of reducing alcohol.

Changes of Chemical Composition and Microflora in Commercial Kimchi (시판 김치의 발효 온도별 성분과 미생물 변화)

  • Shin, Dong-Hwa;Kim, Moon-Sook;Han, Ji-Sook;Lim, Dae-Kwan;Bak, Wan-Soo
    • Korean Journal of Food Science and Technology
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    • v.28 no.1
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    • pp.137-145
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    • 1996
  • Chemical changes, lactic acid bacteria and yeast counts in kimchi prepared by a commercial manufacturer in large scale were monitored at different fermentation temperature. The optimum pH of kimchi, around pH 4.2, reached within 2 days at $25^{\circ}C$, 3 days at $15^{\circ}C$ and 23 days at $5^{\circ}C$ fermentation, respectively. The optimum acidity calculated as lactic acid was not exactly coincident with pH. The total viable count reached at maximum within 2 days at $25^{\circ}C$, 6 days at $15^{\circ}C$ and 12 days at $5^{\circ}C$ fermentation, respectively. The identified strains of Lactobacilli during kimchi fermentation were L. brevis, L. plantarum and L. acidophilus with 3 unidentified strains. L. brevis, L. plantarum appeared from the first stage of fermentation to the terminal at $15^{\circ}C$ and $25^{\circ}C$ with keeping a constant level of viable number. In case of Leuconostoc species, L. mesenteroides subsp. mesenteroides was identified. This strain increased in viable number at the beginning of fermentation and dropped sharply at all fermentation temperatures. Pediococcus species including P. pentosaceus and one unidentified strain increased at the first stage of fermentation and decreased after on. Streptococcus faecium subsp. casseliflavus which appeared at the middle stage and Aerococcus viridans which was sole strain were also confirmed during kimchi fermentation. Cryptococcus laurenti was identified at all fermentation temperature and disappeared at the first stage of fermentation. It was reappeared 10 days only after fermentation at $25^{\circ}C$.

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Electrophoretic Patterns of Sarcoplasmic Proteins in Mid-West Korean Sea Fishes by Thin Layer Polyacrylamide Gel Isoelectrofocusing (등전점 전기영동에 의한 서해산 해산어의 근형단백질의 전기영동에 관한 연구)

  • 김종배;최선남;이근우;정용현
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.20 no.5
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    • pp.455-460
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    • 1991
  • In order to identify hard distinct 12 fish species(shiba shrimp Metapenaeus joyneri, fleshy shrimp Penaeus orientalis, ridgetail prawn Palaemon carinicauda, yellow croaker Pseudosciaena manchurica, croaker Niber albiflora, Colichthyes fragilis, brown sole Limanda herzensteini, frog flounder Pleuronichthys cornutrs, Areliscus rhomaleus, stone flounder Kareius bicoloratus, harvest fish Pampus argenteus, flag fish Goniistius zonatus) by seeing with naked eye in Kunsan coastal area, sarcoplasmic protein in the supernatant was used for isoelectric focusing. For getting supernatant, fish muscle tissue was blended with two times deionized water and centrifuged (at $4^{\circ}C$, 12,000rpm for 15min). Isoelectric focusing of sarcoploasmic protein carried out on a LKB Multiphor II using polyacrylamide gel plate (2mm thickness, pH $3.5~10^{\circ}C$, pH 5~8 gradient, at $10^{\circ}C$ for 1.5, 3 hours). In case of uncertain protein pattern, pH gradient was modified to narrow pH gradiet, and excuted 2-D electrophoresis using conventional polyacrylamide gel electrophoresis. Most of fishes except yellow croaker and Collichthyes fragilis were distingushed by isoelectric focusing. The protein maps of 2-D electrophoresis for analyzing two protein bands at aimilar positions(pH 5, 6) between the two fish species showed the diffeences of the estimated molecular weights, 11,700(pH5.0) and 87,000(pH6.0)

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The Historical Status of "Cheonmyeong-Doseol" by Jeong Ji-wun (추만(秋巒) 정지운(鄭之雲) 저술(著述) 『천명도설(天命圖說)』의 사상사적(思想史的) 위상(位相))

  • Park, Sung-soon
    • (The)Study of the Eastern Classic
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    • no.54
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    • pp.253-288
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    • 2014
  • A pen name Chuman(秋巒; a mountain of Fall), Jeong Ji-wun(1509-1561) is a famous person wrote "Cheonmyeong-Doseol" which made two men, Yi Hwang and Ki Dae-$s{\breve{u}}ng$ dispute Sachil-Nobyeon(traditional argument about heart/mind). But except this point, few people of nowadays know who Jeong Ji-wun was. Therefore this paper is made for understanding Jeong Ji-wun in the point of view in history of thought. "Cheonmyeong-Doseol" by Jeong Ji-wun had systemized traditional theory of Cheonil-Habil(sky and human are an organic body), through this theory, Jeong Ji-wun had taken an important role who developed the Neo-Confucian theory in the field of political thought at that time. All Neo-Confucian literati used the theory of heart/mind as a sole useful method in political field from after King Myeonjong and Seonjo. "Cheonmyeong-Doseol" by Jeong Ji-wun was a first step to this scene. Especially "Cheonmyeong-Doseol" was got famous by arguments of Yi Hwang and Ki $Dae-s{\breve{u}}ng$, great scholars in the history of Chosun Dynasty. They firstly developed Sachil-Nobyeon, Sandan(pure morality) comes from li, Chiljeong(emotions) comes from Ch'i, is it right or not? This argument with "Cheonmyeong-Doseol" raised up the level of Neo-Confucian theory at that time. Like this, "Cheonmyeong-Doseol" was on the first step for Neo-Confucian literati to study and develop their theory of political thought which covered politics in Chosun Dynasty.

Culture Conditions of Garlic Resistant Lactic Acid Bacteria for Feed Additives (사료첨가용 생균제 개발을 위한 마늘 내성 유산균의 배양 조건)

  • Kim, Yu-Jin;Jang, Seo-Jung;Park, Jung-Min;Kim, Chang-Uk;Park, Young-Seo
    • Food Engineering Progress
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    • v.14 no.1
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    • pp.65-74
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    • 2010
  • Culture conditions of L. plantarum TJ-LP-002, the garlic resistant strain isolated from pakimchi (green onion kimchi), were investigated for the use of feed additives. Acetic acid, citric acid, lactic acid, and tartaric acid were detected in the culture supernatant, and especially the concentrations of lactic acid and acetic acid significantly increased during cultivation. The antimicrobial activity of L. plantarum TJ-LP-002 was not affected by proteases, calatase or cellulase, which showed that the antimicrobial activity might be due to the production of acids rather than proteinaceous antimicrobial substances. L. plantarum TJ-LP-002 was resistant to neomycin sulfate, spectinomycin dihydrochloride, and lincomycin hydrochloride, sensitive to streptomycin sulfate, and intermediate resistant to ampicillin trihydrate, chloramphenicol, erythromycin, tetracycline hydrochloride, and kanamycin sulfate. The optimum initial pH of medium, fermentation temperature and time for the cell growth and antibacterial activity were pH 7.0, 30${^{\circ}C}$ and 24hr, respectively. The optimal composition of culture medium for the cell growth and antimicrobial activity was 3%(w/v) glucose as a carbon source, 3%(w/v) yeast extract as a nitrogen source, and manganese sulfate and ammonium citrate as inorganic salts. The combinatorial supplementation of these inorganic salts, rather than sole addition as an inorganic salt, resulted in better antibacterial activity.

Studies on Phytotoxin in Intensively Cultivated Upland Crops -II. Population and identification of soil microorganisms in rhizosphere of upland crops (연작재배지토양(連作栽培地土壤)의 식물독소(植物毒素)에 관(關)한 연구(硏究) -제(第) II 보(報). 작물근권토양(作物根圈土壤)의 미생물분포(微生物分布)에 관(關)한 연구(硏究))

  • Lee, Sang-Kyu;Suh, Jang-Sun;Kim, Young-Sig;Park, Jun-Kyu
    • Korean Journal of Soil Science and Fertilizer
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    • v.20 no.2
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    • pp.179-183
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    • 1987
  • A series of laboratory experiments were conducted to find out the populations and identification of soil bacteria, fungi and their B/F ratio in the rhizosphere of intensively cultivatad hot-pepper, garlic, flower plants, chinese cabbage, and round onion. The results obtained are summarized as follows: 1. The number of bacteria, fungi and their B/F ratio are remarkably lower than that of normal paddy soils. 2. Nitrate reducers and bacteria which utilized simple sugars for their sole carbon source are predominated in the rhizosphere of intensively cultivated upland crops. 3. Alkaligenetic bacteria predominate in rhizosphere of garlic and tomato cultivated upland soils. 4. Genera of Pseudomonas, Xanthomonas, Bacillus, Arthrobacter, and Achromobacterium are the most common species in the rhizosphere of intensively cultivated upland crops and flower plants. 5. Phytotoxin producers such as Stachybotris sp. were identified in all rhizospheres of intensively cultivated upland crops and flower plants. 6. Most common and highest population of soil fungi were obtained for the genera of Penicillium, Humicola, Phoma and Aspergillus in the rhizosphere of intensively cultivated upland crops and flower plants.

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Functional Dyspepsia (기능성 소화불량증)

  • Song, Kyung Ho
    • Korean Journal of Psychosomatic Medicine
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    • v.24 no.1
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    • pp.3-8
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    • 2016
  • Functional dyspepsia is one of the most common bowel disorders as prevalent of 7.7% Korean population. The cardinal manifestations include bothersome postprandial fullness, early satiation, epigastric burning or pain. These features are chronic and should be presented recurrently with no other compatible organic disease to explain the symptoms. Even though it is not life-shortening, functional dyspepsia usually make the health-related quality of life worse especially if other functional bowel disorder coexist. The coexistence of functional bowel disorders is called as 'overlap syndrome'. Anxiety, somatization and insomnia is more prevalent in overlap syndrome compared with sole functional bowel disorder. Therefore, it is worthwhile that physician interviews and elucidates whether the dyspeptic patient had other kinds of functional bowel disorders, and manages the underlying psychotic pathology. Placebo effect is large in functional dyspepsia, and there is only four kinds of prokinetics that is proven to be superior to placebo. Adverse events relating prolonged administration of prokinetics sometimes fatal or irreversible, physician willing to describe prokinetics should be familiar to the possible adverse effects and the relating risk factors. Pathologic acid reflux is not uncommon in functional dyspepsia, and acid-suppressant is equivalent to the prokientics in most of dyspeptic patients.

Transcriptome profiling of rubber tree (Hevea brasiliensis) discovers candidate regulators of the cold stress response

  • Gong, Xiao-Xiao;Yan, Bing-Yu;Hu, Jin;Yang, Cui-Ping;Li, Yi-Jian;Liu, Jin-Ping;Liao, Wen-Bin
    • Genes and Genomics
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    • v.40 no.11
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    • pp.1181-1197
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    • 2018
  • Tropical plant rubber tree (Hevea brasiliensis) is the sole source of commercial natural rubber and low-temperature stress is the most important limiting factor for its cultivation. To characterize the gene expression profiles of H. brasiliensis under the cold stress and discover the key cold stress-induced genes. Three cDNA libraries, CT (control), LT2 (cold treatment at $4^{\circ}C$ for 2 h) and LT24 (cold treatment at $4^{\circ}C$ for 24 h) were constructed for RNA sequencing (RNA-Seq) and gene expression profiling. Quantitative real time PCR (qRT-PCR) was conducted to validate the RNA-Seq and gene differentially expression results. A total of 1457 and 2328 differentially expressed genes (DEGs) in LT2 and LT24 compared with CT were respectively detected. Most significantly enriched KEGG pathways included flavonoid biosynthesis, phenylpropanoid biosynthesis, plant hormone signal transduction, cutin, suberine and wax biosynthesis, Pentose and glucuronate interconversions, phenylalanine metabolism and starch and sucrose metabolism. A total of 239 transcription factors (TFs) were differentially expressed following 2 h or/and 24 h of cold treatment. Cold-response transcription factor families included ARR-B, B3, BES1, bHLH, C2H, CO-like, Dof, ERF, FAR1, G2-like, GRAS, GRF, HD-ZIP, HSF, LBD, MIKC-MADS, M-type MADS, MYB, MYB-related, NAC, RAV, SRS, TALE, TCP, Trihelix, WOX, WRKY, YABBY and ZF-HD. The genome-wide transcriptional response of rubber tree to the cold treatments were determined and a large number of DEGs were characterized including 239 transcription factors, providing important clues for further elucidation of the mechanisms of cold stress responses in rubber tree.

An Analysis on Determinants that Affect the Sale Price of an Office Building in Seoul after Focusing on Strata Property Sales (서울 오피스 빌딩 매매가격 결정요인 분석 : 부분매매를 중심으로)

  • Yu, Myeong Han;Lee, Chang Moo
    • Korea Real Estate Review
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    • v.28 no.2
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    • pp.7-20
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    • 2018
  • This paper has statistically analyzed the determining factors that affect office building sale prices by focusing on strata property sales through the hedonic price function. In this study, 1,171 office building transaction cases were analyzed in Seoul from 2000 to 2017. To determine the influence of various factors on office building sale prices, independent variables included factors that represented macroeconomic characteristics, locational characteristics, physical characteristics, and deal characteristics. The analysis of the strata property sales, which is a major concern in this study, showed that strata property sales enjoyed a discount of about 1.56 million won per pyeong out of the entire sales. In terms of the discount rate, strata property sales were at a 12.6% discount compared to entire property sales, so it was found that strata property sales significantly influenced office building selling price. This is due to the fact that the owner of the strata property encounters more difficulties in distributing cost than the sole proprietor in terms of property rights and the exercise of management rights. The results of this study are expected to contribute in securing transparency in transactions and risk management strategies in the future.

A Comparative Study on the Characteristics of Friction with/without shoes by Analyzing Bio-signals during walking (보행 시 생체신호분석을 통한 신발 착용 유무에 따른 마찰 특성 비교)

  • Oh, Seong-geun;Kim, Jin-Hyun
    • Journal of Convergence for Information Technology
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    • v.8 no.6
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    • pp.59-66
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    • 2018
  • The utilized coefficient of friction (UCOF) as a ratio of the shear force to the normal force on the ground during walking is used to identify the point at which slip is likely to occur. Shoe walking will change the utilized coefficient of friction by shoe design such as sole thickness and hardness, heel shape, and outsole pattern. In this study, subjects are 21 adults (10 female, 11 male, age: $25.2{\pm}2.3yrs$, height: $165.6{\pm}7.2cm$), analysis variables were walking speed, GRF, when the UCOF is maximal, and Tangent of CoP-CoM angle, and correlation analysis with the utilized friction coefficient (UCOF). As a result, First, for the shod walking the time point which UCOF is maximum about heel strike was faster and the magnitude was larger than for barefoot walking. Second, the correlation between the tangent of CoP-CoM and UCOF of right foot was higher at the left heel striking point (UCOF2_h) which occurred in the post propulsion phase than at the right heel striking point (UCOF1_h). This suggests that the right foot UCOF is related to the braking phase of left foot( which is the propulsion phase of right foot) rather than the braking phase of right foot.