• Title/Summary/Keyword: Sole

Search Result 1,488, Processing Time 0.028 seconds

S5 Lipase : An Organic Solvent Tolerant Enzyme

  • Zaliha Raja Noor;Rahman Raja Abdul;Baharum Syarul Nataqain;Salleh Abu Bakar;Basri Mahiran
    • Journal of Microbiology
    • /
    • v.44 no.6
    • /
    • pp.583-590
    • /
    • 2006
  • In this study, an organic solvent tolerant bacterial strain was isolated. This strain was identified as Pseudomonas sp. strain S5, and was shown to degrade BTEX (Benzene, Toluene, Ethyl-Benzene, and Xylene). Strain S5 generates an organic solvent-tolerant lipase in the late logarithmic phase of growth. Maximum lipase production was exhibited when peptone was utilized as the sole nitrogen source. Addition of any of the selected carbon sources to the medium resulted in a significant reduction of enzyme production. Lower lipase generation was noted when an inorganic nitrogen source was used as the sole nitrogen source. This bacterium hydrolyzed all tested triglycerides and the highest levels of pro-duction were observed when olive oil was used as a natural triglyceride. Basal medium containing Tween 60 enhanced lipase production to the most significant degree. The absence of magnesium ions ($Mg^{2+}$) in the basal medium was also shown to stimulate lipase production. Meanwhile, an alkaline earth metal ion, $Na^+$, was found to stimulate the production of S5 lipase.

Degradation of Malic Acid by Issatchenkia orientalis KMBL 5774, an Acidophilic Yeast Strain Isolated from Korean Grape Wine Pomace

  • Seo, Sung-Hee;Rhee, Chang-Ho;Park, Heui-Dong
    • Journal of Microbiology
    • /
    • v.45 no.6
    • /
    • pp.521-527
    • /
    • 2007
  • Several yeast strains degrading malic acid as a sole carbon and energy source were isolated from Korean wine pomace after enrichment culture in the presence of malic acid. Among them, the strain designated as KMBL 5774 showed the highest malic acid degrading ability. It was identified as Issatchenkia orientalis based on its morphological and physiological characteristics as well as the nucleotide sequences of the internal transcribed spacer (ITS) 1-5.8S rDNA-ITS II region. Phylogenetic analysis of the ITS I-5.8S rDNA-ITS II sequences showed that the KMBL 5774 is the closest to I. orientalis zhuan 192. Identity of the sequences of the KMBL 5774 was 99.5% with those of I. orientalis zhuan 192. The optimal pH of the media for the growth and malic acid degradation by the yeast was between 2.0 and 3.0, suggesting that the strain is an acidophile. Under the optimized conditions, the yeast could degrade 95.5% of the malic acid after 24 h of incubation at $30^{\circ}C$ in YNB media containing 2% malic acid as a sole carbon and energy source.

An Experimental Study on the Removal of Chlorophenol by TiO2/H2O2/UV Using Continuous flow Reactor (연속흐름식 반응기를 이용한 TiO2/H2O2/UV에 의한 클로로페놀 제거(除去)에 관한 실험적(實驗的) 연구(硏究))

  • Lee, Sang-Hyup;Park, Joo-Suk;Park, Chung-Hyun;Kim, Dong Ha
    • Journal of Korean Society of Water and Wastewater
    • /
    • v.12 no.3
    • /
    • pp.55-64
    • /
    • 1998
  • The degradation efficiency of chlorophenolic compounds in $TiO_2/H_2O_2$ combined system was compared with that of in $TiO_2$ sole system. As a result, the addition of hydrogen peroxide in photocatalytic oxidation reaction greatly enhanced the degradation efficiency of chlorophenolic compounds due to the availability of the hydroxyl radical formed on the $TiO_2$ surface. The hydrogen peroxide under UV illumination produces hydroxyl radicals that appear to be another source of hydroxyl radical formation. These results indicated the $TiO_2/H_2O_2$ combined system shows higher degradation efficiency than the $TiO_2$ sole system. Compared to another oxidation reaction, hydrogen peroxide assisted photocatalytic oxidation is more promising in practical aspect.

  • PDF

Bilateral Sixth Nerve Palsies as the Sole Manifestation of Atypical Miller-Fisher Syndrome (양측 외전신경마비만을 보인 비전형적 Miller-Fisher 증후군)

  • Nho, Sang-Woo;Kim, Jong-Kuk;Park, Whan-Seok;Jung, Eun-Joo;Kim, Sang-Jin;Kim, Eung-Gyu;Bae, Jong-Seok
    • Annals of Clinical Neurophysiology
    • /
    • v.13 no.1
    • /
    • pp.51-53
    • /
    • 2011
  • Recently, we encountered a man with isolated bilateral sixth nerve palsies and areflexia whose titer of anti-GQ1b IgG antibody was elevated. We propose that bilateral sixth nerve palsies can be the sole manifestation of "anti-GQ1b antibody syndromes" and that patients with isolated bilateral sixth nerve palsies should be administered an anti-GQ1b antibody test for the diagnosis of acute immune-related neuropathy.

The Biomechanical Comparison of Running Shoes According to the Difference of Insole (인솔 차이에 따른 런닝화의 운동역학적 비교)

  • Jin, Young-Wan;Shin, Sung-Hwon
    • Korean Journal of Applied Biomechanics
    • /
    • v.17 no.2
    • /
    • pp.51-59
    • /
    • 2007
  • These studies show that I applied to functional insole (a specific A company) for minimizing shocks and sprain people's ankle arising from running. How to an effect on human body which studied a kinematics and kinetics from 10 college students during experiments. This study imposes several conditions by barefoot, normal running shoes and put functional insole shoes ran under average $2.0{\pm}0.24\;m$/sec by motion analysis and ground reaction force that used to specific A company. First of all, motion analysis was caused by achilles tendon angle, angle of the lower leg, angle of the knee, initial sole angle and barefoot angle. The result of comparative analysis can be summarized as below. Motion analysis showed that statically approximates other results from achilles tendon angle (p<.01), initial ankle angle(p<.05), initial sole angle(p<.001) and barefoot angle(p<.001). Ground reaction force also showed that statically approximates other results from impact peak timing (p<.001), Maximum loading rate(p<.001), Maximum loading rate timing (p<.001) and impulse of first 20 percent (p<.001). Above experiment values known that there was statically difference between Motion analysis and Ground reaction force under absorbing of the functional insole shoes which was not have an effect on our body for kinetics and kinematics.

Isolation and Cultivation of Methanotrophic Consortium for Trichloroethylene Degradation (Trichloroethylene(TCE)의 분해를 위한 메탄자화균총의 분리 및 배양)

  • 이무열;신현재;염상필;양지원
    • KSBB Journal
    • /
    • v.13 no.5
    • /
    • pp.483-490
    • /
    • 1998
  • Two unidentified methanotrophic strains (MM-white and MM-red) secreting soluble methane monooxygenase (sMMO) involved in thrichloroethylene biodegradation have been isolated from mixed methanotrophic consortium (MM) around Taejon area. Subsequently four methanotrophic strains were isolated from MM and named according to their color; white (MS-white), yellow (MS-yellow), pink (MS-pink) and reddish brown (MS-rbrown). All strains except MS-yellow which can take glucose as well as methane, metabolized methane as a sole carbon source. They all showed symbiotic behavior when methane was used as the sole carbon source. Optimum conditions of cell growth for MM were pH of 6.8 - 7.2, temperature of 29 - 32$^{\circ}C$, and gas flow rate of 6 (for methane), 40 (for air), and 4 ml/min (for carbon dioxide). The sMMO activity was expressed as naphthalene oxidation rate (${\mu}$mol/ mg protein/ hr). The sMMO activity for MM grown in flask culture with 1 ${\mu}$M of CuSO4 was 36, while it was 61 without copper. The activity for MM grown in the fermentor without CuSO4 was 1077, but is was 197 after reaction with 5 ppm of TCE. The methanotrophs showed significantly high sMMO activity despite the presence of 1 ${\mu}$M of CuSO4, although most of other strains already known could not express sMMO activity under this condition.

  • PDF

The Effects of Pilates based Breathing on Changes in the Thicknesses of the Abdominal Muscles

  • Kwon, Hae-Yeon;Moon, Hyun-Ju;Kim, Moon-Jeoung
    • Journal of the Korean Society of Physical Medicine
    • /
    • v.11 no.3
    • /
    • pp.59-63
    • /
    • 2016
  • PURPOSE: The purpose of the present study is to examine the effects of sole Pilates based breathing on the thicknesses of the abdominal muscles. METHODS: Twenty adults aged in their 20-30s were randomly selected. The selection criteria were those who had no medical history of pain due to musculoskeletal system, neurologic, or orthopedic diseases within six months before the selection. The thicknesses of the abdominal muscles (the transversus abdominis muscle, internal oblique abdominal muscle, and external oblique abdominal muscle) of the subjects were measured using ultrasonography before and after Pilates breathing. The study was approved by the research ethics committee of the Catholic University of Pusan. RESULTS: After Pilates based breathing, the thicknesses of the transversus abdominis muscle, the internal oblique abdominal muscle, and the external oblique abdominal muscle increased significantly. In particular, the thickness increase rate of the transversus abdominis muscle was shown to be the highest. CONCLUSION: Sole Pilates based breathing is expected to be effective for the improvement of trunk stability through strengthening of abdominal muscles, because it induces the coordinated contraction of the transversus abdominis muscle and other abdominal muscle. It will be need the Longitudinal study for identify to long term effects of Pilates based breathing.