• Title/Summary/Keyword: Solanum tuberosum L.

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감자(Solanum tuberosum L.)와 담배 (Nicotiana tabacum L.)의 원형질체 배양 및 융합 (Culture and Fusion of Protoplasts from Potato (Solanum tuberosum L.) and Tobacco (Nicotiana tabacum L.))

  • 정상호
    • Journal of Plant Biology
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    • 제30권4호
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    • pp.287-298
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    • 1987
  • The regenerative capacities of protoplasts isolated from potato (Solamum tuberosum L.) tubers and tobacco (Nicotiana tabacum L.) mesophyll tissues were examined, and then their intergeneric protoplast fusion was carried out. The potato tuber-derived protoplasts proliferated into the calli some of which showed rudimentary shoot-like structures, which had not been attempted before from tubers, while the tobacco protoplasts were regenerated into the whole plants. Intergeneric protoplast fusion between potato and tobacco was carried out and the heteroplasmic fusion products were formed. The first cell division of some of them was observed after 5 days of culture.

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감자(Solanum tuberosum L.) 괴경의 배양세포로부터 원형질체의 분리 (Isolation of Protoplasts from Cultured Cells of Potato (Solanum tubersoum L.) Tuber Tissue)

  • 정상호
    • Journal of Plant Biology
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    • 제29권1호
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    • pp.11-18
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    • 1986
  • Protopasts were isolated from cultured cells of potato (Solanum tuberosum L.) tuber tissue. The ability of callus formation from the culture cells was higher in cultivars Dejima and Superior than in Shimabara and Irish Cobbler on Lam's medium. Therefore, the former was used as sources for protoplast isolation. Friable calli were transferred to liquid media and cells in exponential phase were used for protoplast isolation. In both of Dejima and Superior, the yield of protoplasts was high in the enzyme solution of 2% Onozuka cellulase and 1% macerozyme. Also, viability of isolated protoplasts was very good. Thus, it seems that these protoplasts would be applicable to various aims of research.

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Molecular Cloning and Expression of Dihydroflavonol 4-reductase Gene in Tuber Organs of Purple-fleshed Potatoes

  • Kang, Won-Jin;Lee, Yong-Hwa;Kim, Hyun-Soon;Joung, Hyouk;Jeon, Jae-Heung
    • Journal of Applied Biological Chemistry
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    • 제49권3호
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    • pp.75-81
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    • 2006
  • A full-length cDNA encoding dihydroflavonol 4-reductase (st-dfr) of potato was isolated by rapid amplification of cDNA ends, and their expression was investigated from purple-fleshed potato (Solanum tuberosum L. cv. Jashim). The st-dfr exists as a member of a small gene family and its transcripts was abundant in the order of tuber flesh, stem, leaf, and root. The expressions of st-dfr gene were light inducible and cultivar dependant. Transgenic potato plants harboring antisense st-dfr (AS-DFR) sequences were analyzed. The accumulation of mRNA was nearly completely inhibited as a result of introducing an AS-DFR gene under the control of the 35S CaMV promoter into the red tuber skin Solanum tuberosum L. cv. Desiree. The anthocyanin content of the tuber peels of the transgenic lines was dramatically decreased by up to 70%. The possible production of flavonols in the peels of AS-DFR transgenic potatoes was discussed.

감자 (Solanum tuberosum L.) CycD3유전자의 분리 및 특성 분석 (Isolation and Characterization of a cDNA Encoding CycD3 Gene from Potato(Solanum tuberosum L.))

  • 강인홍;최승호;이홍근;황현식;이석찬;정태영;임학태;배신철
    • Journal of Plant Biotechnology
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    • 제30권4호
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    • pp.329-334
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    • 2003
  • D-type cyclin은 호르몬과 영양분의 영향을 받아 세포주기의 G1기에서 S기로 전환을 조절하는 인자이다. 우리는 감자에서 이 유전자를 분리 해냈고, 염기서열 분석을 통하여 D3 cyclin으로 분류하였다. 그리고 StCycD3;1이라 명명하였다. 다른 D cyclin유전자가 세포 분열이 활발한 조직에서 발현되는 것과 같이 StCycD3;1은 감자의 괴경, 뿌리, 꽃, 잎, 줄기, 뿌리줄기, 복지에서 다양한 발현 양상을 보였고, 영양분의 하나인 sucrose에 의하여 발현이 유도되는 것을 확인하였다.

양액재배방식이 '대지' 감자(Solanum tuberosum L.)의 생육 형 소괴경 형성에 미치는 영향 (Effect of Solution Culture System on Growth and Mini-tuber Yield of Hydroponically Grown Potato(Solanum tuberosum L. cv. Dejima))

  • 김기택;박용봉
    • 생물환경조절학회지
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    • 제6권3호
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    • pp.198-204
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    • 1997
  • 감자 소괴동 산을 위해 적합한 양담재배방식을 구명코자 분무경, NFT, perlite 및 scoria 배지비을 이용하여 '대지' 품종을 공시하고 생육과 소엽도의 연성 및 준양에 미치는 영향을 조사한 결과는 다음과 같다. 1. 분무병 방식이 NFT 방식이나 Perlite 및 scoria 배지경에 비해 정식 후 40일과 60일의 지상부 생조이 좋았고, 정식후 90일에는 동윤이 65.4cm, 주당자육가 7.3개로서 가장 많았으며, scoria 배지경은 기윤이 49.5cm, 도상기육 3.2개로서 가장 적었다. 2. 포지장은 분무비에서 30.4cm로 가장 길었고, 주당각지육는 NFT 방식에서 10.5개로 많았다. 3. 정식 후 90일에 주당 괴경수는 분무경에서 67.1개, NFT 62.5개, Perlite 배지경 20.1개, scoria 배지경 18.0개였다. 4. 3g 이상의 괴대 크기 분포는 분무경 72.7%, NFT 62.6%, perlite 배지비 84.1%, scoria 배지경 83.3%로 고협배지경이 높은 분포를 보였으나 주당 괴동수가 적기 때문에 총수양 은 적었다. 5. Perlite 배지경에서 황산된 소괴경은 피일발이이 적고 표피가 매끄러워서 보질의 괴대이 생산되었다.

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Cloning and Characterization of GDP-mannose Pyrophosphorylase from Solanum Tuberosum L.

  • Hyun, Tae-Kyung;Lim, Jung-Dae;Kim, Jae-Kwang;Seong, Eun-Soo;Lee, Jae-Geun;Yoon, Byeong-Sung;Kim, Myong-Jo;Cho, Dong-Ha;Yu, Chang-Yeon
    • 한국약용작물학회지
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    • 제13권5호
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    • pp.276-283
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    • 2005
  • Ascorbic acid is a great antioxidant and helps protect the body against pollutants. GDP-mannose pyrophosphorylase (GMPase) is a key enzyme in manufacturing GDP-mannose, a glycosyl donor for ascorbate and cell wall biosynthesis as well as for protein glycosylation. In this study, we described molecular cloning of a full-length cDNA from Potato (Solanum tuberosum L. cv. Jasim), using tuber. The cDNA isolated encoded a GDP-mannose pyrophosphrylase. The nucleotide sequence of pGMPC showed about 95%, 89% and 80% homology with S. tuberosum (AF022716), N. tabacum (AB066279) and A. thaliana (AF076484) cDNAs clone known as GMPase, respectively. We detected the expression of GMPase using RT-PCR. The highest expression of GMPase was found in stems, and the largest amount of ascorbic acid was also presented in stems. In contrast, the leaf showed minimal level of GMPase transcript and ascorbic acid content. We propose that GMPase expression patterns were similar to the changes of ascorbic acid content in the leaves treated with diverse stresses.

Characterization of Polyphenoloxidase extracted from Solanum tuberosum Jasim

  • Jang, Jae-Won;Ma, Yu-Hyun;Shin, Ju-Mi;Song, Kyung-Bin
    • Food Science and Biotechnology
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    • 제14권1호
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    • pp.117-122
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    • 2005
  • Polyphenoloxidase (PPO) was extracted from Solanum tuberosum Jasim by various chromatographic methods and was subsequently purified and characterized. PPO was purified upto 78-fold from the crude extract. SDS-PAGE profile of the enzyme showed a major subunit of PPO with molecular weight of 40 kDa. The optimum pH and temperature for the maximum activity of PPO was 6.5 and $25^{\circ}C$, respectively. The enzyme was found to be quite stable between 10 and $40^{\circ}C$, whereas it was almost inactivated at $70^{\circ}C$ when incubated for 30 min. Substrate specificity study indicated that catechol was the most suitable substrate for PPO isolated from purple-fleshed potato with a $K_m$ value of 21.1 mM. The most effective inhibitor was ascorbic acid, followed by L-cysteine, citric acid, EDTA, and boric acid. Studies on the effect of metal ion on PPO activity showed that magnesium and copper were inhibitory, while iron and zinc ions increased the activity of PPO.