• Title/Summary/Keyword: Solanum

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Biocontrol of Late Blight and Plant Growth Promotion in Tomato Using Rhizobacterial Isolates

  • Lamsal, Kabir;Kim, Sang Woo;Kim, Yun Seok;Lee, Youn Su
    • Journal of Microbiology and Biotechnology
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    • v.23 no.7
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    • pp.897-904
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    • 2013
  • Seven bacterial isolates (viz., AB05, AB10, AB11, AB12, AB14, AB15, and AB17) were derived from the rhizosphere and evaluated in terms of plant growth-promoting activities and the inhibition of Phytophthora infestans affecting tomatoes in Korea. According to 16S rDNA sequencing, a majority of the isolates are members of Bacillus, and a single isolate belongs to Paenibacillus. All seven isolates inhibited P. infestans by more than 60% in vitro. However, AB15 was the most effective, inhibiting mycelial growth of the pathogen by more than 80% in vitro and suppressing disease by 74% compared with control plants under greenhouse conditions. In a PGPR assay, all of the bacterial isolates were capable of enhancing different growth parameters (shoot/root length, fresh biomass, dry matter, and chlorophyll content) in comparison with non-inoculated control plants. AB17-treated plants in particular showed the highest enhancement in fresh biomass with 18% and 26% increments in the root and shoot biomass, respectively. However, isolate AB10 showed the highest shoot and root growth with 18% and 26% increments, respectively. Moreover, the total chlorophyll content was 14%~19% higher in treated plants.

The Characteristics and Flora of Changwon and Nam Stream Located in Gyeongsangnam-do (경상남도 창원천과 남천의 관속식물상과 특성)

  • Park, Kyung-Hun;You, Ju-Han;Yoon, Young-Chul
    • Journal of the Korean Society of Environmental Restoration Technology
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    • v.13 no.5
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    • pp.12-27
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    • 2010
  • The purpose of this study is carried out to present the raw data for restoring and maintaining an urban stream by objective surveying and analysing about flora in Changwon stream and Nam stream, Changwon-si, Gyeongsangnam-do, Korea. The results are as follows. The numbers of vascular plants were summarized as 248 taxa; 71 families, 184 genera, 220 species, 26 varieties and 2 forma. The numbers of vascular plants by streams were 202 taxa in Changwon stream and 206 taxa in Nam stream. The endemic plant was Salix pseudolasiogyne. The rare plants designated by Korea Foret Service were 2 taxa; Aristolochia contorta and Koelreuteria paniculata. The specific plants by floristic region were 12 taxa; Aphananthe aspera, Artemisia selengensis, Indigofera pseudotinctoria and so forth. The naturalized plants were 37 taxa; Ailanthus altissima, Amaranthus patulus, Amorpha fruticosa and so forth. The invasive alien plants designated by Ministry of Environment were 4 taxa; Ambrosia artemisiifolia var. elatior, Aster pilosus, Rumex acetocella and Solanum carolinense. The UI (Urbanized Index) and NI (Naturalized Index) were 13.6% and 18.3% in Changwon stream and 13.6% and 17.9% in Nam stream.

Genome-Wide SNP Calling Using Next Generation Sequencing Data in Tomato

  • Kim, Ji-Eun;Oh, Sang-Keun;Lee, Jeong-Hee;Lee, Bo-Mi;Jo, Sung-Hwan
    • Molecules and Cells
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    • v.37 no.1
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    • pp.36-42
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    • 2014
  • The tomato (Solanum lycopersicum L.) is a model plant for genome research in Solanaceae, as well as for studying crop breeding. Genome-wide single nucleotide polymorphisms (SNPs) are a valuable resource in genetic research and breeding. However, to do discovery of genome-wide SNPs, most methods require expensive high-depth sequencing. Here, we describe a method for SNP calling using a modified version of SAMtools that improved its sensitivity. We analyzed 90 Gb of raw sequence data from next-generation sequencing of two resequencing and seven transcriptome data sets from several tomato accessions. Our study identified 4,812,432 non-redundant SNPs. Moreover, the workflow of SNP calling was improved by aligning the reference genome with its own raw data. Using this approach, 131,785 SNPs were discovered from transcriptome data of seven accessions. In addition, 4,680,647 SNPs were identified from the genome of S. pimpinellifolium, which are 60 times more than 71,637 of the PI212816 transcriptome. SNP distribution was compared between the whole genome and transcriptome of S. pimpinellifolium. Moreover, we surveyed the location of SNPs within genic and intergenic regions. Our results indicated that the sufficient genome-wide SNP markers and very sensitive SNP calling method allow for application of marker assisted breeding and genome-wide association studies.

Development of Calculating System of Solids Level to Harvest High Solids Potato (Solanum tuberosum L.)

  • Jung, Jae-Youn;Suh, Sang-Gon
    • Horticultural Science & Technology
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    • v.31 no.1
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    • pp.103-109
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    • 2013
  • Estimating the high tuber solids needs a simulation system on potato growth, and its development should be obtained by using agricultural elements which analyze the relationship between crop growth and agricultural factors. An accurate simulation to predict solids level against climatic change employs a calculation of in vivo energy consumption and bias for growth and induction shape in a slight environmental adaptation. So, to calculate in vivo energy consumption, this study took a concept of estimate of the amount of basal metabolism in each tuber. In the validation experiments, the results of measuring solid accumulation of potatoes harvested at dates suggested by simulation agreed with the actual measured values in each regional field during the growth period of years from 2006 till 2010. The mean values of tuber solids level and inter-annual level variation in validation experiments were predicted well by the simulation model. And also, the results of validation experiments represent that concentration of tuber solids were due mainly to the duration of sunshine, above 190 hours per a month, and the cumulative amount of radiation, above 2,200 $MJ{\cdot}m^{-2}$, of the effective growth period.

A High Yield and Processing Potato Cultivar 'Taedong Valley'

  • Dhital, Shambhu P.;Lian, Yu J.;Hwang, Won N.;Lim, Hak T.
    • Korean Journal of Breeding Science
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    • v.42 no.3
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    • pp.207-211
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    • 2010
  • 'Taedong Valley' is a high yielding and processing potato cultivar, which is a clonal selection resulting from a cross between 'W870' and 'A88431-1'. It is a medium maturating cultivar with medium plant height and light green foliage. 'Taedong Valley' has profuse flowering habit and light purple flowers. Tubers are smooth, round, and with yellow skin, light yellow flesh, medium eye depth. Tubers have medium dormancy and good keeping quality. 'Taedong Valley' has stable yield under wide range of climatic conditions. It is resistant to common scab and potato virus Y, but moderately susceptible to late blight. It is also resistant to most of the disorders, particularly dehiscence, hollow heart and internal brown spots. This cultivar has high level of tuber uniformity and capable of yielding 43.6 t/ha which is about 9.0% higher than the control potato cultivar 'Atlantic' under optimum agronomical practices.

Analysis of Genes Activated by Salt and ER Stress in bZIP17 and bZIP28 Gene Transgenic Potato Plants

  • Kim, Kyung Hwa;Choi, Man Soo;Chun, Jae Buhm;Jin, Mi Na;Jeong, Nam Hee;Kim, Dool Yi
    • Proceedings of the Korean Society of Crop Science Conference
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    • 2018.10a
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    • pp.179-179
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    • 2018
  • Potato (Solanum tubersosum L.) is susceptible to various environmental stresses such as salt, high temperature, and drought. Especially, potato tuber growth is greatly affected by drought that causes not only yield reduction but also loss of tuber quality. Since unpredictable global weather changes cause more severe and frequent water limiting conditions, improvement of potato drought tolerance can minimize such adverse effects under drought and can impact on sustainable potato production. Genetic engineering can be utilized to improve potato drought tolerance, but such approaches using endogenous potato genes have rarely been applied. We were obtained AtbZIP28 gene transgenic potato plants. It is identified transcript levels at various stress conditions, polyethylene glycol (PEG), NaCl, (ABA). Also, For identification to regulate ER stress response genes in AtbZIP28 gene transgenic potato plant, we screened seven potato genes from RNA-seq analysis under TM treatment. Five and two genes were up- and down-regulated by TM, respectively. Their expression patterns were re-examined at stress agents known to elicit TM, DTT, DMSO and salt stress.

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Encapsulation of Anthocyanin from Purple Potato by the Application of Food Polymers

  • Azad, Obyedul Kalam;Cho, Dong Ha;Park, Cheol Ho
    • Proceedings of the Korean Society of Crop Science Conference
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    • 2018.10a
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    • pp.42-42
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    • 2018
  • Anthocyanins has a strong antioxidant capacity but exhibit poor stability in water. Therefore, stability of anthocyanin from purple potato (Solanum tuberosum L.) was encapsulated by the application of food polymers. Solid formulation of purple potato was prepared using whey protein, tapioca and lecithin by capillary rheometer at $80^{\circ}C$. The ratio of the polymer and potato powder was 2:8. Total phenolic compound, total flavonoid, total anthocyanin and antioxidant activity was investigated by the spectrophotometer. Result revealed that total phenolic compound (TP) ($5321{\mu}g/100g$), total flavonoid (TF) ($1352{\mu}g/100g$) total anthocyanin (TA) ($764{\mu}g/100g$) and free radical antioxidant activity (DPPH) (86%) was higher in 0.01 M acetic acid mediated lecithin based formulation compared to control (Potato powder) (TP: $1357{\mu}g/100g$; TF) ($634{\mu}g/100g$, TA) ($264{\mu}g/100g\;DPPH$) (64%). Lecithin is a strong emulsifier having capacity to extract bioactive compound and encapsulate extracted compound by nonpolar tail and negatively charged head. Therefore, it would be concluded that lecithin might be used as an encapsulating agent for the bioactive compound from purple potato.

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Encapsulation of Anthocyanin from Purple Potato by the Application of Food Polymers

  • Azad, Obyedul Kalam;Cho, Dong Ha;Park, Cheol Ho
    • Proceedings of the Korean Society of Crop Science Conference
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    • 2018.10a
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    • pp.274-274
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    • 2018
  • Anthocyanins has a strong antioxidant capacity but exhibit poor stability in water. Therefore, stability of anthocyanin from purple potato (Solanum tuberosum L.) was encapsulated by the application of food polymers. Solid formulation of purple potato was prepared using whey protein, tapioca and lecithin by capillary rheometer at $80^{\circ}C$. The ratio of the polymer and potato powder was 2:8. Total phenolic compound, total flavonoid, total anthocyanin and antioxidant activity was investigated by the spectrophotometer. Result revealed that total phenolic compound (TP) ($5321{\mu}g/100g$), total flavonoid (TF) ($1352{\mu}g/100g$) total anthocyanin (TA) ($764{\mu}g/100g$) and free radical antioxidant activity (DPPH) (86%) was higher in 0.01 M acetic acid mediated lecithin based formulation compared to control (Potato powder) (TP: $1357{\mu}g/100g$; TF) ($634{\mu}g/100g$, TA) ($264{\mu}g/100g\;DPPH$) (64%). Lecithin is a strong emulsifier having capacity to extract bioactive compound and encapsulate extracted compound by nonpolar tail and negatively charged head. Therefore, it would be concluded that lecithin might be used as an encapsulating agent for the bioactive compound from purple potato.

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Changes in flavor-relevant compounds during vine ripening of tomato fruit and their relationship with ethylene production

  • Wang, Libin;Luo, Weiqi;Sun, Xiuxiu;Qian, Chunlu
    • Horticulture, Environment, and Biotechnology : HEB
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    • v.59 no.6
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    • pp.787-804
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    • 2018
  • Flavor quality is import for determining consumer perception and acceptance of tomato products. In this study, 'Fendou' tomato fruit were harvested at six ripening stages and sampled to investigate the development of flavor-relevant compounds during vine ripening. Results showed that upon the initiation of ripening there was an increase in respiration rate and concomitant ethylene evolution that was associated with increased membrane permeability. In accordance with these physiological changes, flavor-relevant compounds demonstrated different expression patterns as fruit ripened, which contributed to 'red-ripe' flavor characteristics of red-ripe fruit. Based on correlation analysis between ethylene evolution and the flavor-relevant compounds during 'Fendou' tomato ripening and the other researchers' reports, the activation of System 2-dependent autocatalytic ethylene production plays an important role in the development of most flavor-relevant compounds during tomato vine ripening. Overall, our results suggested that most flavor-relevant compounds that accumulated the most during tomato fruit ripening at red stage could be under ethylene regulation and were among the most important contributors to the 'red-ripe' flavor. Due to the development of these compounds, the flavor quality at late ripening stages is different from that of fruit at early ripening stages.

Naturalized Plants and Their Characteristics in Nakdong River Ecological Park in Busan Metropolitan City - Focused on Eulsukdo, Maekdo and Samnak ecological parks - (부산광역시 낙동강 생태공원의 귀화식물상과 특성 - 을숙도생태공원, 맥도생태공원, 삼락생태공원을 중심으로 -)

  • Gwak, Su-Bin;Jeong, Jae-Hyun;You, Ju-Han
    • Journal of the Korean Society of Environmental Restoration Technology
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    • v.24 no.1
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    • pp.81-96
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    • 2021
  • The purpose of this study was conducted in order to provide the necessary basic data, to establish management solutions and to improve biodiversity by calculating similarity index, urbanization index (UI), and disturbed index (DI) to understand current status of naturalized and invasive alien plants in Eulsukdo, Maekdo and Samnak ecological parks in Busan, South Korea. The numbers of naturalized plants identified in these parks were 76 taxa; 20 families, 53 genera, and 76 species. As a result of the similarity index analysis, the most similarity level (83.0%) was obtained at Eulsukdo and Maekdo parks. The numbers of invasive plants identified in the two parks were 11 taxa; Rumex acetosella L., Sicyos angulatus L., Solanum carolinense L., Ambrosia artemisiifolia L., Ambrosia trifida L., Hypochaeris radicata L., Lactuca serriola L., Solidago altissima L., Symphyotrichum pilosum (Willd.) G.L.Nesom, Paspalum distichum L., and Humulus scandens (Lour.) Merr. Overall, UI and DI were 28.6% and 66.7%, respectively, indicating that the ecosystem disruption was serious.