• Horticultural Science & Technology
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• v.31 no.3
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• pp.328-336
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• 2013

This study was conducted to evaluate imported tomato $F_1$ cultivars as breeding materials for the resistance to Tomato yellow leaf curl virus (TYLCV) by molecular markers and bioassay. From marker genotyping and disease evaluation of 40 $F_1$ cultivars, most of the cultivars declared as TYLCV-resistance carried heterozygous marker genotype for the TYLCV resistance genes Ty-1, Ty-3, or Ty-3a, and showed low disease rates. Whereas, 4 of 5 $F_1$ cultivars declared as intermediate resistance showed marker genotype for susceptibility and disease rates ranged 18.1-33.3%. However, the xx cultivars showed inconsistency in marker genotype and disease rate. Characterization of horticultural traits of the $F_1$ cultivars with TYLCV-resistance indicated that large-size fruit cultivars were higher in yield and similar in sugar contents and solid-acid ratio compared to a control cultivar preferred in the domestic market, although hardness remained to be a problem. On the other hand, cherry tomato cultivars showed lower yield and brix, but longer internode compared to a control cultivar, indicating that breeding for TYLCV-resistance using these cultivars will require more efforts and time compared to large-sized.

• Journal of Bio-Environment Control
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• v.21 no.1
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• pp.12-19
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• 2012

This research was performed to determine the appropriate daily last irrigation time to enhance the plant growth and the water and fertilizer use efficiencies in coir bag culture for tomato plant. The time to finish the daily irrigation was set by 1, 2, 3 and 4 hours before the sunset. The water content in the substrate was greatly affected by the last irrigation time. The earlier the last time, the greater the daily fluctuation of water contents in the substrate. The daily irrigation times were not affected by using irrigation management system controlled by drainage electrodes or the physiochemical properties of coir. The growth characteristics were not significantly different among the treatments. The highest marketable yields were obtained in the treatment finishing two hours before sunset, and the lowest yields were obtained in the the treatment finishing 4 hours before sunset. Based on the result from surveying quantity of irrigated water for 128 days of the experiment period, the water and fertilizer use efficiencies were lowest in the treatment finishing 4 hours before sunset, and the highest in the treatment finishing 2 hours before sunset. In terms of plant growth, yields, water and fertilizer use efficiencies, 2 hours before sunset treatment was determined as the most economical and desirable irrigation schedule.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.49 no.6
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• pp.447-451
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• 2004

This study was conducted to establish the proper techniques of the recirculating wick hydroponics for safe seed tuber proliferation of potatoes (Solanum tuberosum L. CV. Dejima). To achieve these, several intact tubers (5, 10 and 20 g) and cut seed-pieces (two or four) were treated in wick hydroponic system beds. A polystyrene box (31cm in width, 20cm in height, 51cm in length, and $0.031m^3$ in volume) was placed on a styrofoam hox. Eight wicks $(width\;1.5cm\;{\times}\;length\;40cm) $ were put into each polystyrene hox and the boxes were filled with perlite + peatmoss (1 : 2, v/v) medium. Top fresh weight per plant increased with increasing the tuber size from 10 to 30g/tuber. As the tuber size increases from 10 to 30 g/tuher in case of uncut tuber, the marketable tuber (>5g) production per plant increased from 83.8 to 141.8 g/plant and the marketable tuber (>5g) production per plant of cut tuber was slightly higher than that of uncut treatment. Total tuber yield ranged from 4.16 to $6.56kg/m^2$ and the percentage of seed tubers was greater than $97\%$ for all treatments. These results indicate that seed tuber should be cut to produce large tuber $(\geq10g)$ in the recirculating wick hydroponic system.

• Research in Plant Disease
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• v.23 no.3
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• pp.228-233
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• 2017

Six commercial rootstock varieties were tested for resistance to bacterial wilt (Ralstonia solanacearum) by dipping the roots of seedlings in the bacterial suspension at seedling stage. 'Shincheonggang' and 'B-Blocking' ranked top and second in resistance to bacterial wilt, respectively. For disease index of the grafted plants, 'Dotaerang' grafted onto 'Shincheonggang' was the least, followed by 'Ultra', 'Fighting', 'B-Blocking', 'Special', and 'Connection' grafts. Thus, disease development on the grafted plants was correlated with resistance of rootstocks. The effect of rootstocks on horticultural characteristics including days to flower of scion was minimum or negligible. In correlation between horticultural characteristics of rootstocks and scions grafted thereon, statistically significant negative correlation was observed in days to flower and fruit height but no significant correlation was observed in yield per plant, fruit diameter and soluble solid content in the fruits. Thus, selection of resistant rootstock varieties that support the growth and yield of scion well is necessary for effective control of the disease and yield increase.

• Korean Journal of Plant Tissue Culture
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• v.24 no.2
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• pp.71-75
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• 1997

For the establishment of early selection of heat-tolerant clones through in vitro tuberization of true potato seeds, different temperature treatments for in vitro tuberization were investigated. The ratios of tuberization at 2$0^{\circ}C$ on var. Superior and DTO-33 treated with 5 mg/L of BAP and 500 mg/L of CCC, were 85% and 92%, respectively. At 3$0^{\circ}C$, the ratio of tuberization on DTO-33 was 37%, which revealed strongly heat-tolerant clone. In culture system of in vitro tuberization, the number of tubers per flask at 2$0^{\circ}C$ on non-subculture incubation was more than that on subculture incubation. The condition of non-subculture and short-day treatment for 4 weeks was good for production as 10.6 tubers per flask, which was very similar to that of long-day treatment. On the other hand, tuber diameter on long-day treatment was greater as 11.2 mm than on short-day treatment. Therefore, in vitro tuberization from true potato seeds could be induced under the condition of long-day treatment at darkness.

• Korean Journal of Plant Tissue Culture
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• v.24 no.2
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• pp.77-81
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• 1997

For the early selection of heat-tolerant clones, the true potato seeds of 750 clones were obtained by several cross combinations : $CIP\;575015\;{\times}\;katahdin,\;CIP\;575015\;{\times}\;B6603-6,\;84\;I\;35-4\;{\times}\;katahdin,\;CIP\;575015\;{\times}\;NookSack,\;and\;CIP575015\;{\times}\;Superior.$ The ratios of in vitro tuberization at 3$0^{\circ}C$ were decreased by 43% in all cross combinations compared with at 2$0^{\circ}C$. In particular, tuberization rate of $CIP575015\;{\times}\;katahdin$ cross at 3$0^{\circ}C$ was only 21%. On the other hand, the rate of tuberization of $CIP575015\;{\times}B6603-6$ cross was 58 % at 3$0^{\circ}C$, so this cross combination was thought to be good heat-tolerant clone. To screen the heat-tolerant clones in lower land during high temperature period, microtubers were cultivated in Kangnung, and the characters of tubers and productivity were examined. Among screened clones, one clone at 2$0^{\circ}C$ and three clones at 3$0^{\circ}C$ were shown to be heat-tolerant. The yield of 89ML75-8 was 88% more than that of Superior, and dry weight rate of 89 ML64-11 was 18.8%. Therefore, 89ML64-11 clone was considered as a good cultivar for processing.

• Journal of Plant Biotechnology
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• v.34 no.3
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• pp.253-261
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• 2007

This study was conducted to develop an antibiotics marker-free potato (Solanum tuberosum L., cv. Taedong valley) plant having resistance against two herbicides. Agrobacterium tumefaciens strain EHA105, harboring a binary vector plasmid pCAMBIA3300 containing bar gene under the control of a promoter CaMV35S and linked CP4-EPSPS genes driven by CaMV35S promoter, was used in the current study. The leaf segments of newly bred potato variety (cv. Taedong Valley) was co-cultured with Agrobacterium. Then, the regenerated individual shoots were excised and transferred to potato multiplication medium supplemented with 0.5 mg/L phosphinothricin. The shoots were rooted in MS medium without hormone and obtained putative transgenic plant E3-6. Integration of target genes into the E3-6 plant and their expression was confirmed by PCR, Southern analysis, and ELISA test. The tissue necrosis test on young leaf blade and shikimic acid accumulation test using the tissue of E3-6 plant were conducted to investigate the resistance to glufosinate-ammonium and glyphosate, respectively. The transgenic plants (E3-6) simultaneously showed a high resistance to both herbicides. The same results were surely obtained also in the whole plants foliar-treated with alone or mixture of two herbicides, glufosinate-ammonium and glyphosate.

• Korean Journal of Environment and Ecology
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• v.23 no.6
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• pp.506-515
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• 2009

This study was carried out to investigate the distribution status and UI(urbanization index) of the naturalized plants and exotic plants. For this purpose 7 cities and counties in the northern South Jeolla Province were selected and investigations were conducted on a total of 14 sites, two sites from each city or county, during the period of March to November 2006. The exotic plants were identified as 111 taxa; 26 families, 79 genera, 105 species, and 6 varieties. As for urbanization index, Hampyeong County had the highest level of urbanization(21.95%) and Gokseong County the lowest level of urbanization(16.38%). Naturalized plants classified as family were as follows: Compositae was 36 kinds(32.43%) Graminae 17 kinds(15.31%) and Leguminosae 7 kinds(6.31%). Major communities of naturalized plants consisted of 21 communities including Robinia pseudoacacia-Amorpha fruticosa community, Erigeron annuus community, Erigeron canadensis community etc. Invasive naturalized plants consisted of 7 taxa: Paspalum disticum var. indutum, Paspalum distichum, Rumex acetocella, Solanum carolinense, Aster pilosus, Ambrosia artemisiifolia var. elatior and Ambrosia trifida. The study shows that the integrated management of exotic plants on a national scale is needed. It also recommend that a systematic and integrated management of exotic plants in the northern South Jeolla Province is crucial to preserve and protect natural species.

• Journal of Plant Biotechnology
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• v.47 no.2
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• pp.157-163
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• 2020

Calmodulin (CaM) mediates cellular Ca²⁺ signals in the defense responses of plants. We previously reported that GmCaM-4 and 5 are involved in salicylic acid-independent activation of disease resistance responses in soybean (Glycine max). Here, we generated a GmCaM-4 cDNA construct under the control of the cauliflower mosaic virus (CaMV) 35S promoter and transformed this construct into potato (Solanum tuberosum L.). The constitutive over-expression of GmCaM-4 in potato induced high-level expression of pathogenesis-related (PR) genes, such as PR-2, PR-3, PR-5, phenylalanine ammonia-lyase (PAL), and proteinase inhibitorII (pinII). In addition, the transgenic potato plants exhibited enhanced resistance against a bacterial pathogen, Erwinia carotovora ssp. Carotovora (ECC), that causes soft rot disease and showed spontaneous lesion phenotypes on their leaves. These results strongly suggest that a CaM protein in soybean, GmCaM-4, plays an important role in the response of potato plants to pathogen defense signaling.

• Research in Plant Disease
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• v.20 no.4
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• pp.253-258
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• 2014

This study was conducted to evaluate tomato plant resistance against bacterial wilt by Ralstonia solanacearum using tomato cultivars or tomato breeding lines maintained in RDA-Genebank of Rural Development Administration and to select resistant tomato lines for breeding purpose. We evaluated the disease responses of a total of 13 cultivars and 39 breeding lines from RDA-Genebank using R. solanacearum SL341 strain, which is a representative strain in Korea. Tomato cultivar Hawaii 7996 and Moneymaker were used as a resistant control plant and a susceptible control plant, respectively. A total of 32 cultivars were susceptible and 10 cultivars showed various disease response suggesting resistant phenotype segregation in the lines. Five commercial cultivars and 5 breeding lines exhibited strong resistance to bacterial wilt by the SL341 strain. These 5 breeding lines might be used for further study of plant defense response against bacterial wilt and cloning of the resistance gene from tomato plants. Ultimately, the selected lines could be used for tomato breeding to generate bacterial wilt resistant tomato plants.