• Journal of Bio-Environment Control
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• v.31 no.4
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• pp.300-310
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• 2022

The objective of this experiment was to investigate the effect of drip irrigation volume on tomatoes (Solanum lycopersicum L.) grown in a greenhouse using perlite medium. Plants were treated by three different irrigation treatment I₀, I₂₅, and I₅₀ (where irrigation volume of I₂₅ and I₅₀ was 25% and 50% higher than I₀, having limited or no leaching). Growth characteristics of plants, yield and water use efficiency were measured. The result showed that plant height, leaf length and leaf width were lowest in the I₀ treated plants. However, these parameters were not statistically significant differences between the plants that were grown in the I₂₅ and I₅₀ treatment. Soluble solids content, acidity and dry matter of 111th, 132nd, and 143rd days harvested tomato were higher in the plants irrigated with lowest volume (I₀) than the higher volume (I₂₅ or I₅₀). In addition, water content was lower in the 111th and 132nd days of harvested tomatoes from the I₀ treatment. The number of big-size tomatoes (>180 g) was significantly higher in the I₂₅ irrigated plants. There was no significant difference in the total number of harvested fruits among the treatments. The average fruit weight and total yield of harvested tomatoes were lowest in the I₀ treated plants. The water consumption of tomato was not significantly different amongst the treatments but water use efficiency was lowest in the I₀ treatment. Principal component analysis revealed that total soluble solid and acidity of tomato showed a positive correlation between each other. These results suggest that I₂₅ was the optimum irrigation treatment for tomato based on its measured growth characteristics, yield and water use efficiency.

• Journal of Life Science
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• v.33 no.6
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• pp.498-505
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• 2023

Solanum tuberosum Linnaeus cv Hongyoung, which represents red potato, was developed in Korea. Hongyoung is known to have anti-oxidant, anti-inflammatory, anti-viral, and anti-tumor properties, but no research has been conducted on the growth inhibition and apoptosis effects of hongyoung in YD-10B oral cancer cells. In this study, the combined treatment of hongyoung ethanol extract (HEE) and cisplatin were examined to determine its ability to inhibit cancer cell growth, induce apoptosis, and inhibit matrix metalloproteinases (MMP)-2 and MMP-9 cancer metastasis. The cell viability was investigated using a 2-(2-methoxy-4-nitrophenyl)-3-(4-nitrophenyl)-5-(2,4-disulfophenyl)-2H- tetrazolium monosodium salt (MTS) assay, and the ability to induce apoptosis was analyzed using an FACS analyzer. The mRNA expression and protein activity of MMP-2 and MMP-9 were measured via RT-PCR and zymography. The YD-10B oral cancer cells showed an increase in growth inhibition as the concentration of HEE increased. The combination of 200 µM cisplatin and 500 ㎍/ml HEE reduced the growth of the YD-10B oral cancer cells by more than 50% compared to cisplatin alone. When phorbol 12-myristate 13-acetate (PMA)-treated YD-10B oral cancer cells were co-treated with 200 µM cisplatin and 500 ㎍/ml HEE, both the mRNA expression and protein activity of the MMP-2 and MMP-9 decreased. In addition, the percentage of the sub-G1 phase, which indicates apoptosis ability, more than doubled when treated in combination with 200 µM cisplatin and 500 ㎍/ml HEE than when cisplatin alone was used. The results of this study therefore suggest the possibility of using a combination of HEE and cisplatin in the development of effective drugs to treat oral cancer.

• The Journal of Natural Sciences
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• v.4
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• pp.103-142
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• 1991

These studies were carried out to select somatic hybrid using selectable marker genes of Nicotiana glauca transformed by NPTII gene and Solanum tuberosum transformed by T- DNA, and to study characteristics of transformant. The results are summarized as follows. 1. Crown gall tumors and hairy roots were formed on potato tuber disc infected by A. tumefaciens Ach5 and A. rhizogenes ATCC15834. These tumors and roots could be grown on the phytohormone free media. 2. Callus formation from hairy root was prompted on the medium containing 2, 4 D 2mg/I with casein hydrolysate lg/l. 3. The survival ratio of crown gall tumor callus derived from potato increased on the medium containing the activated charcoal 0. 5-2. 0mg/I because of the preventions on the other hand, hairy roots were necrosis on the same medium. 4. Callus derived from hairy root were excellently grown for a short time by suspension culture on liquid medium containing 2, 4-D 2mg/I and casein hydrolysate lg/l. 5. The binary vector pGA643 was mobilized from E. coli MC1000 into wild type Agrobacteriurn tumefaciens Ach5, A. tumefaciens $A_4T$ and disarmed A. tuniefaciens LBA4404 using a triparental mating method with E. ccli HB1O1/pRK2013. Transconjugants were obtained on the minimal media containing tetracycline and kanamycin. pGA643 vectors were confirmed by electrophoresis on 0.7% agarose gel. 6. Kanamycin resistant calli were selected on the media supplemented with 2, 4-D 0.5mg/1 and kanamycin $100\mug$/ml after co- cultivating with tobacco stem explants and A. tumefaciens LBA4404/pGA643, and selected calli propagated on the same medium. 7. The multiple shoots were regenerated from kanamycin resistant calli on the MS medium containing BA 2mg/l. 8. Leaf segments of transformed shoot were able to grow vigorusly on the medium supplemented with high concentration of kanamycin $1000\mug$/ml. 9. Kanamycin resistant shoots were rooting and elongated on medium containing kanamycin $100\mug$/ml, but normal shoot were not. 10. For the production of protoplast from potato calli transformed by T-DNA and mesophyll tissue transformed by NPTII gene, the former was isolated in the enzyme mixture of 2.0% celluase Onozuka R-10, 1.0% dricelase, 1.0% macerozyme. and 0.5M mannitol, the latter was isolated in the enzyme mixture 1.0% Celluase Onozuka R-10, 0.3% macerozyme, and 0.7M mannitol. 11. The optimal concentrationn of mannitol in the enzyme mixture for high protoplast yield was 0.8M at both transformed tobacco mesophyll and potato callus. The viabilities of protoplast were shown above 90%, respectively. 12. Both tobacco mesophyll and potato callus protoplasts were fused by using PEG solution. Cell walls were regenerated on hormone free media supplemented with kanamycin after 5 days, and colonies were observed after 4 weeks culture.

• Journal of Biosystems Engineering
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• v.33 no.2
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• pp.106-114
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• 2008

This study was performed to analyze the distribution of air current speed, $CO_2$ concentration, and photosynthetic photon flux (PPF) in a closed transplants production system (CTPS) for producing quality transplants. And the effect of PPF on the growth of potato (Solanum tuberosum L. cv. Dejima) plug seedlings was analyzed. Uniformity of the air current speed in CTPS was improved by installing perforated floors in duct for air circulating and by adjusting of air flow rate of the fan connected to air conditioning unit used in this study, Measured $CO_2$ concentrations were measured $409{\pm}13$, $950{\pm}25$, and $1,550{\pm}35\;{\mu}mol{\cdot}mol^{-1}$ for setting values of 400, 950, and $1,550\;{\mu}mol{\cdot}mol^{-1}$, respectively. Uniformity of PPF by adding each one the single fluorescent lamp of 20 W at both ends of the single fluorescent lamps of 40 W was highly improved. While the average PPF measured under the twin fluorescent lamps of 55 W installed at regular intervals of 10 cm was decreased by increasing the vertical distance from the lighting sources, the ratio of average PPF measured at both ends to PPF measured in the center was 74-79%. Five levels ($100{\pm}9$, $150{\pm}14$, $200{\pm}17$, $250{\pm}24$ and $300{\pm}31{\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}$) of PPF were provided to investigate the effect of PPF on plant height, fresh weight and dry weight of potato plug seedlings produced in CTPS. Plant height was decreased by increasing PPF. Maximum fresh weight and dry weight were shown under PPF of $250{\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}$. Thus PPF of $250\;{\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}$ was enough to produce quality potato transplants under air temperature, photoperiod, and relative humidity of $20^{\circ}C$, 16/8 h, and 70%, respectively. It was concluded that quality indices such as plant height, fresh weight and dry weight could be improved by illuminating of adequate PPF from artificial lighting sources.

• Korean Journal of Environment and Ecology
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• v.27 no.3
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• pp.280-304
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• 2013

This study was carried out to investigate the flora of Mt. Gilsangsan (Ganghwa-Isl.). The vascular plants were collected 14 times (from March to November 2012), and were identified as 595 taxa; 122 families, 359 genera, 529 species, 7 subspecies, 51 varieties, 2 forms and 3 hybrids. The plant formation of Mt. Gisangsan is the deciduous broad-leaved and conifer mixed forest which is the common one in the middle part of Korean peninsula. All most mountain covered with young secondary forest which is mainly composed of Pinus and Quercus. The family represented by the largest number of species diversity were Asteraceae (11.%), Poaceae (10.7%), Cyperaceae (6.38%), Fabaceae (3.86%) and Rosaceae (3.52%) respectively. Korean endemic plants of this area were 12 taxa; Asarum misandrum, Clematis brachyura, Philadelphus schrenkii, Forsythia koreana, Polygonatum infundiflorum, etc. The red list of vascular plants according to IUCN valuation basis were examined, endangered species (EN) Abies koreana, Near Threatened species (NT) both Wikstroemia trichotoma and Sparganium erectum, Least Concern species (LC) Platycladus orientalis, Swertia diluta var. tosaensis and Iris minutoaurea and by Not Evaluate species (NE) Polygonatum infundiflorum. The floristic regional indicator plants found in this area were 45 taxa comprising two taxa of grade V and VI, nine taxa of grade III, three taxa of grade II, 29 taxa of gradeI. The naturalized plants were identified as 64 taxa and the percent of naturalized index (NI) was 10.7% of total 595 taxa vascular plants. Ecosystem disturbance wild plants were consisted of eight taxa; Rumex acetosella, Sicyos angulatus, Solanum carolinense, Ambrosia artemisiifolia, Ambrosia trifida, Aster pilosus, Hypochaeris radicata and Lactuca scariola. Furthermore, therophytes (26.5%), hemicryptophytes (26.0%), Geophyte (14.0%) showed high proportional ratio in life form spectrum.

• Horticultural Science & Technology
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• v.19 no.4
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• pp.455-458
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• 2001

To screen potato clones with high resistance to hollow heart (HH) and internal brown spot (IBS), field conditions were set up to induce high frequencies of physiological disorders in 'Atlantic' potato through various treatments of mulching, periodic shadings, and plant growth regulators such as trinexapac-ethyl (Tr-E) and dicloprop-triethanol mine (DTA). IBS occurrence was as high as 67.5% in the field plot mulched with transparent film and shaded for 10 days beginning at 80 days after planting. The highest level of HH was 22.9% in the plot mulched with transparent film and shaded for 10 days beginning at 60 days after planting. Very high level of IBS (66.3%) also occurred in the plot treated with 1500 mg/L of Tr-E 40 days after planting, while HH occurred by 21.3% in the plot treated with 1000 mg/L of DTA 70 days after planting. In the plots which were treated with 1,500 mg/L of Tr-E after 40 days of planting and 1000 mg/L of DTA after 70 days of planting, 'Superior' (moderately highly resistant) and 'Atlantic' (very susceptible) could be clearly distinguished to be resistant and susceptible. High occurrence condition set up in this study could be applied for the potato breeding program to screen potato clones with high resistance to HH and IBS.

• Horticultural Science & Technology
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• v.19 no.4
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• pp.459-464
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• 2001

Leaf disks from cultivar 'Kennebec' and one selection line (ND 860-2) were cultured on Murashige-Skoog medium with various combinations of indole acetic acid (IAA) and zeatin riboside. Shoots, roots and callus were induced at various combinations of plant growth regulator levels. The medium containing $3.5mg{\cdot}L^{-1}$ IAA and $4.0mg{\cdot}L^{-1}$ zeatin riboside produced the most plantlets. Rooted regenerants were grown in the greenhouse. The growth of regenerated plants obtained from the MS medium supplemented with $7.0mg{\cdot}L^{-1}$ IAA and $3.0mg{\cdot}L^{-1}$ zeatin riboside was significantly greater than those grown from nodal expalnts. In ND 860-2, a leaf chimera with chlorophyll deficient (light yellow) sectors was found in plants regenerated fiom leaf disks (grown on MS medium supplemented with $3.5mg{\cdot}L^{-1}$ IAA and $3.0mg{\cdot}L^{-1}$ zeatin riboside) but not in plants grown from nodal explants. The phenotypic variability was also observed in the tuber number, size and weight.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.60 no.2
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• pp.231-238
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• 2015

This study was carried out to determine environmental factors affecting the anthocyanin content of color-fleshed potato (Solanum tuberosum L.) tubers. After planting of two color-fleshed potato cultivars of 'Hongyoung' and 'Jayoung' in different 14 locations, their soil chemical properties and meteorological data were evaluated, and anthocyanin contents of tubers were analyzed after harvest, additionally their relationship among them was analyzed through correlation analysis. In comparison with two cultivars, purple-fleshed 'Jayoung' potatoes showed higher anthocyanin content than red-fleshed 'Hongyoung' in almost locations. When locations were divided to three categories (highland, sub-highland and lowland) according to altitude, in general, highland-grown tubers had the higher content of anthocyanin compared to those grown in lowland. An analysis of the results of chemical components of soil showed that anthocyanin content of color-fleshed potato tubers was negatively correlated with the pH of soil. In addition, mean temperature and minimum temperature from 80 to 100 days after planting most significantly affected on the accumulation of anthocyanin in color-fleshed potato tubers, that is, higher content of anthocyanin was observed in tubers produced in locations with lower mean temperature and minimum temperature from 80 to 100 days after planting. This information can be useful to producers and industries in selection of proper fields for the production of color-fleshed potato tubers having high quality in Korea.

• Research in Plant Disease
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• v.18 no.4
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• pp.304-309
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• 2012

Fusarium wilt, caused by three races of Fusarium oxysporum f. sp. lycopersici, is one of the most important disease of tomato (Solanum lycopersicum) worldwide. A total of 1,906 tomato accessions were screened for the resistance to Fusarium wilt using I-3 SNP marker and high resolution melting analysis. Results showed that 97 accessions were homozygous resistant, 8 accessions were heterozygous resistant and 1,801 were homozygous susceptible. Accessions containing resistance were identified in 65 accessions of S. lycopersicum var. lycopersicum, 13 accessions of S. lycopericum var. cerasiform, 8 accessions of S. pimpinellifolium, 3 accessions of S. habrochaites, 3 accessions of S. corneliomulleri, 1 accession of S. galapagense, 3 accessions of S. peruvianum, 1 accession of S. chilense. For accurate evaluation of the Fusarium wilt resistance, however, screening to race 1 and race 2 and bio-assay still remain to be evaluated.

• Journal of Sasang Constitutional Medicine
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• v.9 no.2
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• pp.301-312
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• 1997

Since new continental was discovered, potatoes and sweet potatoes originated from America have been known worldwide. Through literary consideration about potatoes and sweet potatoes, several conclusons were obtained as enumerated below. 1. Based on work done by taxonomist in Orient, both of sweet potatoes and potatoes which were classified to the families of Convovulaceae and Solansceae repectively were included in Seo-rue(薯類). In contrast, both plants' identities were differed systematically. 2. Because potatoes fulfilled with like-cold keep warm, potatoes help So-um-in(少陰人) achieve Yang-nan-ji-gi(陽煖之氣). Meanwhile, the characteristic of sweet potatoes typically in wild living style assists Tae-um-in(太陰人) to own Ho-san-ji-gi(呼散之氣). That sweet potatoes affect on bodies as much as Mar(山藥=마) does makes this pant be defined as good food. 3. The inside contents of Nam-bang-bon-cho-sang(南方本草狀) cited from sweet potatoes chapter in the book of Bon-cho-gang-mok(本草綱目) indicated that the word of sweet potatoes was not found, but called as a kind of Mar(山藥=마) which lived wildly in a mountain Unfortunately, due to that medical scientist cited contents of Nam-bang-bon-cho-sang(南方本草狀) without pursuing carefully, sweet potatoes have been misunderstood. 4. According to the book of Sasang Constitutional medicine, sweet potatoes are good for Tae-um-in(太陰人) who has constipation, and potatoes are good for So-um-in(少陰人) who suffers form stomach illnesses like ulcer and hotburn. Therefore two plants have been used for curing those illness. ln addition, the taking oriental medicine with those plants are recommenced for dietary treatment according to the Constitution.