• Journal of Plant Biotechnology
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• v.45 no.2
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• pp.110-116
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• 2018

This study was conducted to develop a simple, rapid, and reliable method for in vitro propagation of disease-free and true-to-type clones from sucker explants of thornless blackberry (Rubus fruticosus L. ${\times}$ R. parvifolius L.). To induce multiple shoots, the sucker explants were sterilized in 1% NaOCl solution, and then were aseptically cultured on the full and 1/2 MS solid medium supplemented with BAP (0.1, 0.5, 1.0, 2.0 mg/L). After six weeks of culture, the highest frequency (85.4%) of shoot formation from sucker explants was obtained on the full-strength MS medium with 1.0 mg/L BAP. Node explants obtained from multiple shoots were cultured on the various media of full- or half-strength of AD, B5, MS, SH, QL, WPM media, respectively. After 30 days of culture, plant growth was good on the half-AD, half-QL medium. After 90 days of culture, plant growth was good on the full MS and full SH medium. The survival rate of the plantlets after transfer to plastic pots containing soil mixture (sand: soil: vermiculite was 1:1:1, vol.) in the greenhouse was 98%. The results indicate that a multiple-shoot procedure can be applied for an efficient mass propagation of Rubus fruticosus L. ${\times}$ R. parvifolius L.

• Journal of Plant Biotechnology
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• v.45 no.3
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• pp.171-182
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• 2018

Papaya (Carica papaya L.) is one of the crops widely planted in tropical and subtropical areas. The papaya fruit has low calories and are plentiful in vitamins A and C and in minerals. A major problem in papaya production is a plant disease caused by the papaya ringspot virus (PRSV). The first PRSV-resistant GM papaya expressing a PRSV coat protein gene was developed by USA scientists in 1992. The first commercial GM papaya cultivars derived from the event was approved by the US government in 1997. Development of transgenic papayas has been focused on vaccine production and limited agricultural traits, including insect and pathogen resistance, long shelf life, and aluminum and herbicide tolerance. Approximately 17 countries, including the USA and China, produced transgenic papayas and/or commercialized them, which provoked studies on biosafety assessment and development of GM-detection technologies. For the biosafety assessment of potential effects on human health, effects of long-term feeding to model animals have been studied in terms of toxicity and allergenicity. Studies on environmental safety assessment include influence on soil-microbial biodiversity and transfer to soil bacteria of GM selection markers. Many countries, such as Korea, the European Union, and Japan, that have strict regulations for GM crops have serious concerns about unintended introduction of GM cultivars and food commodities using unauthorized GM crops. Transgene- and/or GM event-specific molecular markers and technologies for genomics-based detection of unauthorized GM papaya have been developed and have resulted in the robust detection of GM papayas.

• Korean Journal of Soil Science and Fertilizer
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• v.44 no.6
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• pp.1185-1194
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• 2011

This study was carried out to estimate carbon emission using LCA (Life Cycle Assessment) and to establish LCI (Life Cycle inventory) DB for lettuce production system in protected cultivation. The results of data collection for establishing LCI DB showed that the amount of fertilizer input for 1 kg lettuce production was the highest. The amounts of organic and chemical fertilizer input for 1 kg lettuce production were 7.85E-01 kg and 4.42E-02 kg, respectively. Both inputs of fertilizer and energy accounted for the largest share. The amount of field emission for $CO_2$, $CH_4$ and $N_2O$ for 1 kg lettuce production was 3.23E-02 kg. The result of LCI analysis focused on GHG (Greenhouse gas) showed that the emission value to produce 1 kg of lettuce was 8.65E-01 kg $CO_2$. The emission values of $CH_4$ and $N_2O$ to produce 1 kg of lettuce were 8.59E-03 kg $CH_4$ and 2.90E-04 kg $N_2O$, respectively. Fertilizer production process contributed most to GHG emission. Whereas, the amount of emitted nitrous oxide was the most during lettuce cropping stage due to nitrogen fertilization. When GHG was calculated in $CO_2$-equivalents, the carbon footprint from GHG was 1.14E-+00 kg $CO_2$-eq. $kg^{-1}$. Here, $CO_2$ accounted for 76% of the total GHG emissions from lettuce production system. Methane and nitrous oxide held 16%, 8% of it, respectively. The results of LCIA (Life Cycle Impact assessment) showed that GWP (Global Warming Potential) and POCP (Photochemical Ozon Creation Potential) were 1.14E+00 kg $CO_2$-eq. $kg^{-1}$ and 9.45E-05 kg $C_2H_4$-eq. $kg^{-1}$, respectively. Fertilizer production is the greatest contributor to the environmental impact, followed by energy production and agricultural material production.

• Korean Journal of Soil Science and Fertilizer
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• v.43 no.6
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• pp.892-897
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• 2010

LCA (Life Cycle assessment) was carried out to estimate on carbon footprint and to establish of LCI (Life Cycle Inventory) database of sweetpotato production system. Based on collecting the data for operating LCI, it was shown that input of organic fertilizer was value of 3.26E-01 kg $kg^{-1}$ and it of mineral fertilizer was 1.02E-01 kg $kg^{-1}$ for sweetpotato production. It was the highest value among input for sweetpotato production. And direct field emission was 2.47E-02 kg $kg^{-1}$ during sweetpotato cropping. The result of LCI analysis focussed on greenhouse gas (GHG) was showed that carbon footprint was 4.05E-01 kg $CO_2$-eq. $kg^{-1}$ sweetpotato. Especially $CO_2$ for 71% of the GHG emission and the value was 2.88E-01 kg $CO_2$-eq. $kg^{-1}$ sweetpotato. Of the GHG emission $CH_4$, and $N_2O$ were estimated to be 18% and 11%, respectively. It might be due to emit from mainly fertilizer production (32%) and sweetpotato cultivation (28%) for sweetpotato production system. $N_2O$ emitted from sweetpotato cultivation for 90% of the GHG emission. With LCIA (Life Cycle Impact Assessment) for sweetpotato production system, it was observed that the process of fertilizer production might be contributed to approximately 90% of GWP (global warming potential). Characterization value of GWP and POCP were 4.05E-01 $CO_2$-eq. $kg^{-1}$ and 5.08E-05 kg $C_2H_4$-eq. $kg^{-1}$, respectively.

• Korean Journal of Soil Science and Fertilizer
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• v.43 no.6
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• pp.898-903
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• 2010

This study was carried out to estimate carbon emission using LCA (Life Cycle Assessment) and to establish LCI (Life Cycle Inventory) database of soybean production system. Based on collecting the data for operating LCI, it was shown that input of organic fertilizer was value of 3.10E+00 kg $kg^{-1}$ soybean and it of mineral fertilizer was 4.57E-01 kg $kg^{-1}$ soybean for soybean cultivation. It was the highest value among input for soybean production. And direct field emission was 1.48E-01 kg $kg^{-1}$ soybean during soybean cropping. The result of LCI analysis focussed on greenhouse gas (GHG) was showed that carbon footprint was 3.36E+00 kg $CO_2$-eq $kg^{-1}$ soybean. Especially $CO_2$ for 71% of the GHG emission. Also of the GHG emission $CH_4$, and $N_2O$ were estimated to be 18% and 11%, respectively. It might be due to emit from mainly fertilizer production (92%) and soybean cultivation (7%) for soybean production system. $N_2O$ was emitted from soybean cropping for 67% of the GHG emission. In $CO_2$-eq. value, $CO_2$ and $N_2O$ were 2.36E+00 kg $CO_2$-eq. $kg^{-1}$ soybean and 3.50E-01 kg $CO_2$-eq. $kg^{-1}$ soybean, respectively. With LCIA (Life Cycle Impact Assessment) for soybean production system, it was observed that the process of fertilizer production might be contributed to approximately 90% of GWP (global warming potential). Characterization value of GWP was 3.36E+00 kg $CO_2$-eq $kg^{-1}$.

• Journal of Korean Society of Forest Science
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• v.111 no.3
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• pp.405-417
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• 2022

The purpose of this study was to describe the photosynthetic features of Prunus maximowiczii and Prunus serrulate Lindl. var. pubescens (Makino) Nakai in response to drought stress. Specifically, we studied the effects of drought on photosynthetic ability and photosystem II activity. Drought stress (DS) was induced by cutting the water supply for 30 days. DS decreased the moisture contents in the soil, and between the 10th and 12th days of DS, both species had 10% or less of x., After the 15th day of DS, it was less than 5%, which is a condition for disease to start. We observed a remarkable decrease of maximum photosynthesis rate starting from 10th day of DS; the light compensation point was also remarkable. Dark respiration and net apparent quantum yield decreased significantly on the 15th day of DS, and then increased on the 20th day. In addition, the stomatal transpiration rate of P. maximowiczii decreased significantly on the15th day of DS, and then increased on the 20th day. Water use efficiency increased on the 15th day of DS, and then decreased on the 20th day. The stomatal transpiration rate of P. serrulate decreased significantly on the 20th day of DS, and then increased afterward, while its water use efficiency increased on the 20th day of DS, and then decreased afterward. These results indicate that the closure of stoma prevented water loss, resulting in a temporary increase of water use efficiency. Chlorophyll fluorescence analysis detected remarkable decreases in the functional index (PI_ABS) and energy transfer efficiency in P. maximowiczii after the 15th day of DS. Meanwhile, photosystem II activity decreased in P. serrulate after 20 days of DS. In addition, Ts-Ta, PI_ABS, DI_O/RC, ET_O/RC followed similar trends as those of the soil moisture content and photosynthetic properties, indicating that they can be used as useful variables in predicting DS in trees.

• Korean Journal of Agricultural Science
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• v.8 no.2
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• pp.195-202
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• 1981

The return of water to the atmosphere from water, soil and vegetation surface is one of the most important aspects of hydrological cycle, and the seasonal trend of variation of river basin evaporation is also meaningful in the longterm runoff analysis for the irrigation and water resources planning. This paper has been prepared to show some imformation to estimate the monthly river basin evaporation from pan evaporation, potential evaporation, regional evaporation and temperature through the comparison with river basin evaporation derived from water budget method. The analysis has been carried out with the observation data of Yongdam station in the Geum river basin for five year. The results are summarized as follows and these would be applied to the estimation of river basin evaporation and longterm runoff in ungaged station. 1. The ratio of pan evaporation to river basin evaporation ($E_w/E_{pan}$) shows the most- significant relation at the viewpoint of seasonal trend of variation. River basin evaporation could be estimated from the pan evaporation through either Fig. 9 or Table-7. 2. Local coefficients of cloudness effect and wind function has been determined to apply the Penman's mass and energy transfer equation to the estimation of river basin evaporation. $R_c=R_a(0.13+0.52n/D)$ $E=0.35(e_s-e)(1.8+1.0U)$ 3. It seems that Regional evaporation concept $E_R=(1-a)R_C-E_p$ has kept functional errors due to the inapplicable assumptions. But it is desirable that this kind of function which contains the results of complex physical, chemical and biological processes of river basin evaporation should be developed. 4. Monthly river basin evaporation could be approximately estimated from the monthly average temperature through either the equation of $E_w=1.44{\times}1.08^T$ or Fig. 12 in the stations with poor climatological observation data.

• Korean Journal of Environment and Ecology
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• v.30 no.1
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• pp.110-117
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• 2016

Litter fall is a source of nutrients and carbon transfer in terrestrial ecosystems. Litter decomposition provides nutrients needed for plant growth, sustains soil fertility, and supplies $CO_2$ to the atmosphere. We collected the leaf litter of evergreen broadleaf tree, Camellia japonica L., and carried out a decomposition experiment using the litterbag method in Ju-do, Wando-gun, Korea for 731 days from Dec 25, 2011 to Dec 25, 2013. The leaf litter of C. japonica remained 42.6% of the initial litter mass after experiment. The decay constant (k) of C. japonica leaf litter was $0.427yr^{-1}$. The carbon content of C. japonica leaf litter was 44.6%, and the remaining carbon content during the decomposition tended to coincide with the changes in litter mass. The initial nitrogen and phosphorus content was 0.47% and 324.7 mg/g, respectively. The remaining N in decaying litter increased 1.66-fold in the early decomposition stage, then gradually decreased to 1.18-fold after 731 days. The content of P showed the highest value (1.64-fold of initial content) after 456 days, which then fell to a 1.15-fold after 731 days. The remaining Ca, K, Mg and Na content in C. japonica leaf litter tended to decrease during decomposition. The remaining K showed a remaining mass of 8.9% as a result of rapid reduction. The initial C/N and C/P ratio of C. japonica leaf litter was 94.87 and 1368.5, respectively. However, it tended to decrease as decomposition progressed because of the immobilization of N and P (2.78 and 2.68-fold of initial content, respectively) during the leaf litter decaying. The study results showed that N and P was immobilized and other nutrients was mineralized in C. japonica leaf litter during experimental period.

• Korean Journal of Agricultural and Forest Meteorology
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• v.12 no.4
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• pp.307-316
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• 2010

Complex terrain refers to irregular surface properties of the earth that influence gradients in climate, lateral transfer of materials, landscape distribution in soils properties, habitat selection of organisms, and via human preferences, the patterning in development of land use. Complex terrain of mountainous areas represents ca. 20% of the Earth's terrestrial surface; and such regions provide fresh water to at least half of humankind. Most major river systems originate in such terrain, and their resources are often associated with socio-economic competition and political disputes. The goals of the TERRECO-IRTG focus on building a bridge between ecosystem understanding in complex terrain and spatial assessments of ecosystem performance with respect to derived ecosystem services. More specifically, a coordinated assessment framework will be developed from landscape to regional scale applications to quantify trade-offs and will be applied to determine how shifts in climate and land use in complex terrain influence naturally derived ecosystem services. Within the scope of TERRECO, the abiotic and biotic studies of water yield and quality, production and biodiversity, soil processing of materials and trace gas emissions in complex terrain are merged. There is a need to quantitatively understand 1) the ecosystem services derived in regions of complex terrain, 2) the process regulation occurred to maintain those services, and 3) the sensitivities defining thresholds critical in stability of these systems. The TERRECO-IRTG is dedicated to joint study of ecosystems in complex terrain from landscape to regional scales. Our objectives are to reveal the spatial patterns in driving variables of essential ecosystem processes involved in ecosystem services of complex terrain region and hence, to evaluate the resulting ecosystem services, and further to provide new tools for understanding and managing such areas.

• Korean Journal of Plant Resources
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• v.18 no.1
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• pp.1-7
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• 2005

This study was conducted to investigate the possibility of cultivating tea plants planted in the mountain area of west Gyeongnam province, Korea for 2 years from March 27th, 2001 to July 30th 2003. Ninety each plants collected from 5 different sife were cultivated in nursery cup pot$(\phi\;16cm)$. in the greenhouse condition and transplanted in 5 different location and monitored their survival growing state etc. The results obtained are as follow : 1. Survival rate tea plants after transfer to soil was relatively high Gaya-myeon Hanyang-gun with $90.0\%$ and the lowest in Buksang-meyon Geochang-gun with $80.0\%$. The tea plant collected from Gaya-myeon showed the best growing activity at early stage. 2. For the second year of harvesting time, survival rate was the highest in Machun-myeon Hamyang-gun with $92.2\%$ and the lowest was Buksang-myeon Geochang-gun with $76.7\%$. 3. For the 3rd year of harvesting time, it was impossible to data collection because of the most upper parts of plants were killed by severe freezing weather condition. In the Baekjeon-myeon, Hamyang-gun Buksang-myeon, Ungyang-myeon Geochang-gun, which are severly cold$(below\;-10^{\circ}C)$ in winter season, seems not a suitable places for tea plant cultivation since it is very different to harvest the young loaves in growing season. In conclnsion we could select two sites Gaya-myeon, Hapcheon-gun, Machun-myeon, Hamyang-gun, as tea plant cultivation in the mountain area of west-Gyeongnam province, korea.