• Proceedings of the Korea Water Resources Association Conference
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• 2021.06a
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• pp.128-128
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• 2021

Most of the studies about stormwater low impact development technologies used generalized observations without fully understanding the mechanisms affecting the whole performance of the systems from catchment to the facility itself. At present, these LID technologies have been treated as black box due to fluctuating flow and environmental conditions affecting its operation and treatment performance. As such, the implications of microbial community to the overall performance of the tree-box filter were investigated in this study. Summer season was found to be the most suitable season for microorganism growth since more microorganism were found during this season. Least microorganism count was found in spring because of the plant growth during this season since plant penology influences the seasonal dynamics of soil microorganisms. Litterfall during fall season might have affected the microorganism count during winter since, during this season, the compositional variety of soil organic matter changes affecting growth of soil microbial communities. Microbial analyses of sediment samples collected in the system revealed that the most dominant microorganism phylum is Proteobacteria in all the seasons in both inlet and outlet comprising 37% to 47% of the total microorganism count. Proteobacteria was followed by Acidobacteria, Actinobacteria and Chloroflexi which comprises 6% to 20%, 9% to 20% and 2% to 27%, respectively of the total microorganism count for each season. These findings were useful in optimizing the design and performance of tree box filters considering physical, chemical and biological pollutant removal mechanisms.

• Journal of Soil and Groundwater Environment
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• v.6 no.2
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• pp.49-56
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• 2001

Benzene, one of the aromatic hydrocarbons, can be degraded by physical, chemical and biological processes in aquifers. This study aimed at analyzing separately the three different forms of degradation by performing column tests. Column tests using KCl and benzene as tracers were conducted for four different cases: 1) no hydrogen peroxide and no microorganism, 2) hydrogen peroxide only; 3) microorganism only; 4) hydrogen peroxide and microorganism to investigate the sorption and degradation of benzene. The observed BTCs of KCl and benzene in all cases showed that the arrival times of the peaks of both tracers coincided well but the peak concentration of benzene was much lower than that of KCl. This reveals that a predominant process affecting the transport of benzene in a sandy soil is an irreversible sorption and/or degradation rather than retardation. Decay of benzene through sorption and degradation increased with the addition of hydrogen peroxide and/or microorganism. Dissolved oxygen decreased with the increase of benzene in all cases indicating that degradation of benzene was also influenced by dissolved oxygen. For BTCs with the addition of microorganisms (case 3 and case 4), microorganism showed much lower concentrations compared to the initial levels and an increasing tendency with time although concentrations of benzene returned to zero, indicating a possible retardation of microorganism due to reversible and irreversible sorption to the particle surfaces.

• Korean Journal of Soil Science and Fertilizer
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• v.34 no.5
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• pp.365-372
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• 2001

The objective of this study was to determine the effects of various kinds of composts on the change of soil physical properties and microorganism in upland soils. Field experiments were conducted in the loam and sandy loam soils, while the clay loam and sandy loam soils were used for laboratory experiments. Various kinds of composts such as poultry manure compost(PMC). cow manure compost(CMC). human excrement sludge(HES), and food industrial sludge compost(FISE) were applied annually at rates of 0, 40, and $80Mg\;ha^{-1}$ to soils grown with soybean and maize plants for 4 years during 1994 to 1997. The results of this study were as follows : Bulk density of loam soil decreased with compost application to $1.07{\sim}1.32Mg\;m^{-3}$ compared with $1.49Mg\;m^{-3}$ of control plot, while in sandy loam soil it decreased to $1.00{\sim}1.20Mg\;m^{-3}$ compared with $1.25Mg\;m^{-3}$ of control plot. Bulk density of soil was decreased according to maize cultivation compared with bare control, but soybean cultivation was similar. Population of organic material decomposing microorganisms was increased rapidly at the initial incubation stage at $25^{\circ}C$, and increased more sensitively at the loam soil than sandy loam soil. In the case of the change of microorganisms associated with nitrogen circulation, ammonia oxidizing bacteria was more at the initial incubation stage, and denitrifying bacteria was more at the initial incubation stage, and denitrifying bacteria increased until 1~4 weeks after incubation and increased more at the loam soil than sandy loam soil.

• Journal of the Korea Organic Resources Recycling Association
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• v.31 no.3
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• pp.73-82
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• 2023

This study was conducted to investigate the effect of soil physico-chemical properties and crop growth responses for application of biochar derived from substrate with post harvest of oyster mushroom. The biochar was produced at 450~600℃ using a top-light up draft gasifier (TLUD) production system. As a result of elemental analysis, the biochar used was C 76.2%, H 2.5%, N 3.2%, and H/C was 0.39, which met the international certification standards for biocarbons (IBI) below 0.7. The chemical properties were 10.1 for pH, 1.0% for P₂O₅, 1.8% for K₂O, and 2.5% for CaO. The application rates of biochar were 0, 100, 200, 300, and 500 kg/10a. For cultivation of chinese cabbage and welsh onion, soil organic matter (OM), total nitrogen (T-N), total carbon (T-C), Ex.cation K contents and cation exchange capacity (CEC) in the treatments were increased compared to the no treatment. In addition, the bulk density was lowered and the porosity was increased, improving the soil physical properties in the treated soil. The growth of chinese cabbage and green onion increased with the application of biochar, but the yields of chinese cabbage and green onion did not significantly different among the treatments. Soil carbon sequestration in the treatments enhanced with increasing the amount of biochar application. It is expected to apply the biochar derived from spent oyster mushroom substrate in the eco-friendly farm soil management, improving soil physico-chemical properties.

• Journal of Microbiology and Biotechnology
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• v.30 no.11
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• pp.1688-1696
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• 2020

Soil physical and chemical characteristics, soil potential denitrification rates (PDR), community composition and nirK-, nirS- and nosZ-encoding denitrifiers were studied by using MiSeq sequencing, quantitative polymerase chain reaction (qPCR), and terminal restriction fragment polymorphism (T-RFLP) technologies base on short-term (5-year) tillage field experiment. The experiment included four tillage treatments: conventional tillage with crop residue incorporation (CT), rotary tillage with crop residue incorporation (RT), no-tillage with crop residue retention (NT), and rotary tillage with crop residue removed as control (RTO). The results indicated that soil organic carbon, total nitrogen and NH₄⁺-N contents were increased with CT, RT and NT treatments. Compared with RTO treatment, the copies number of nirK, nirS and nosZ in paddy soil with CT, RT and NT treatments were significantly increased. The principal coordinate analysis indicated that tillage management and crop residue returning management were the most and the second important factors for the change of denitrifying bacteria community, respectively. Meanwhile, this study indicated that activity and community composition of denitrifiers with CT, RT and NT treatments were increased, compared with RTO treatment. This result showed that nirK, nirS and nosZ-type denitrifiers communities in crop residue applied soil had higher species diversity compared with crop residue removed soil, and denitrifying bacteria community composition were dominated by Gammaproteobacteria, Deltaproteobacteria, and Betaproteobacteria. Therefore, it is a beneficial practice to increase soil PDR level, abundance and community composition of nitrogen-functional soil microorganism by combined application of tillage with crop residue management.

• KSBB Journal
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• v.26 no.1
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• pp.1-6
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• 2011

Contamination of soils, groundwater, air and marine environment with hazardous and toxic chemicals is major side effect by the industrialization. Bioremediation, the application of microorganism or microbial processes to degrade environmental contaminant, is one of the new environmental technologies. Because of low water solubility and volatility of diesel, bioremediation is more efficient than physical and chemical methods. The purpose of this study is biodegradation of diesel in sand by using Rhodococcus fascians, a microorganism isolated from petroleum contaminated soil. This study was performed in the column containing sand obtained from sea sides. Changes in biodegradability of diesel with various flow rates, inoculum sizes, diesel concentrations, and pH were investigated in sand column. The optimal condition for biodegradation of diesel by R. fascians in sand column system was initial pH 8 and air flow rate of 30 mL/min. Higher diesel degradation was achieved at larger inoculum size and the diesel degradation by R. fascians was not inhibited by diesel concentration up to 5%.

• Korean Journal of Environmental Agriculture
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• v.38 no.4
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• pp.262-268
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• 2019

BACKGROUND: To investigate mineralization characteristics of organic resources in the soil, five materials (rice straw, cow manure sawdust compost, microorganism compost, mixed oil-cake, and amino acid fertilizer) were treated according to the nitrogen content, and an indoor incubation experiment was conducted for 128 days. The results of this analysis were applied to determine the nitrogen mineralization pattern of these organic resources. METHODS AND RESULTS: During the constant temperature incubation period, the nitrogen net mineralization rate of the organic resources was the highest in the amino acid fertilizer with the highest nitrogen content, and the lowest in the rice straw with the lowest nitrogen content. A positive correlation (0.96) was observed between the potential nitrogen mineralization rate and total nitrogen content. The mineralization rate constant, k, was negatively correlated with the organic matter (-0.96) and carbon content (-0.97). The nitrogen mineralization rate during the first cropping season, as estimated by the model, was 6.6%, 11.6%, 30.9%, 70.7%, and 81.0% for the rice straw, the cow manure sawdust compost, the microorganism compost, the mixed oil-cake, and the amino acid fertilizer, respectively. CONCLUSION: The nitrogen mineralization rate varies depending on the type of organic resources or the nitrogen content; thus, it can be used as an index for determining the nitrogen supply characteristics of the organic resource. Organic resources such as compost with low nitrogen content or those undergoing fermentation contain organic nitrogen. Organic nitrogen is stabilized during the composting process. Therefore, as the nitrogen mineralization rate of these resources is lower than that of non-fermented organic resources, it is desirable to use the fermented organic materials only to improve soil physical properties rather than to supply nutrients for the required amount of fertilizer.

• Journal of Soil and Groundwater Environment
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• v.15 no.1
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• pp.1-8
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• 2010

Cometabolic air sparging (CAS) is a new and innovative technology that uses air sparging principles but attempts to optimize in situ contaminant degradation by adding a growth substrate to saturated zone. CAS relies on the degradation of the primary growth substrate and cometabolic substrate transformation in the saturated zone and in the vadose zone for volatilized contaminants. In this study, we have investigated to determine MTBE degradation pattern and microbial activity variation if using propane as a primary substrate at the condition of considering air injection rate and air injection pattern. Laboratory-scale two-dimentional aquifer physical model studies were used and the experimental results were represented that the optimal conditions were as air injection rate of 1,000 mL/min and pulsed air injection pattern (15 min on/off). Over 1,000 mL/min air injection rate and continuous air injection pattern was no affected to increase DO concentration. On the other hand, Injection of propane and propane-utilizing bacteria degraded MTBE partially. And also, injection of propane- and MTBE-utilizing bacteria effectively degraded MTBE and TBA production was observed.

• Korean Journal of Heritage: History & Science
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• v.41 no.1
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• pp.99-108
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• 2008

The paleogenetic analysis has become an increasingly important subject of archaeological, anthropological, biological as well as public interest. Recently, scientific research for human skeletal remains was more activated because of increasing awareness of the valuable archaeological information by the ancient DNA analysis. State of preservation of organic remains vary in different soil and burying environmental condition. Almost all available tissue disappear to analysis ancient DNA of bone in acidic soil caused by climate and geological features in Korea. Many preserved human remains excavated in the 'Heogwakmyo'(limelayered tomb of Chosun Dynasty Period) is able to explain through the relationship between burial conditions and bone survival form the burial method and ceremony. Ancient DNA analysis of excavated human bone form ancient tomb requires to remove contaminants such as microorganism's DNA and soil components that affect authentic results. Particularly, contamination control of contemporary human DNA is major serious problem and should verified by criteria of authenticity. In order to understand migration and culture of ancient population, when possible, ancient DNA studies needs to go abreast both radiocarbon and stable isotope studies because the dietary inferences will suggest ancient subsistence and settlement patterns. Also when the paleogenetic research supported with the arts and humanities research such as physical anthropology and archaeology, more valuable ancient genetic information is providing a unique results about evolutionary and population genetics studies to reconstruct the past.

• KSBB Journal
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• v.24 no.5
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• pp.453-457
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• 2009

Contamination of marine environment with hazardous and toxic chemicals is more common these days. Bioremediation is the application of microorganism or microbial processes to degrade environmental contaminant. Because of low water solubility and volatility of diesel, bioremediation is more efficient than physical and chemical methods. The objective of this study is biodegradation of diesel in sea water by using Rhodococcus fascians which is isolated petroleum-contaminated soil. R. fascians was cultured on sea water containing diesel to determine the diesel degradability. Changes in biodegradability of diesel with various inoculum sizes, diesel concentrations, initial pH, and culture temperature were analyzed by TPH analysis using gas chromatography. The inoculum size 2% was effective for biodegrdation of diesel in sea water by R. fascians. When diesel concentration was 5%, the growth of cell was inhibited by the toxicity of diesel. The optimal temperature and initial pH for degradation of diesel in sea water were $27^{\circ}C$ and pH 8.