• Korean Journal of Microbiology
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• v.21 no.1
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• pp.7-14
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• 1983

In order to study the effects of cropping system and fungicide (Dachigaren) on soil microbes, the seasonal fluctuations of soil microbes in the fields of radish and Chinese cabbage including soil pH, Soil moisture content and soil temperature were investigated on every 15 day interval from the begining of March to late October in 1981. The population of total fungus peaked at the begining of July, while that of total bacteria, at the begining of August. They were affected by soil temperature, however pathogenic microbes seemed to be more related with host plants than the soil temperature, because pathogens showed high density through the whole cultivation period. The pathogenic microbes showed the density of order ; Xanthomonas, Erwinia, Pseudomonas, Agrobacterium and Corynebacterium. Xanthomonas, Erwinia and Pseudomonas, which induced radish and Chinese cabbage diseases were higher than Agrobacterium and Corynebacterium in population densigy. Bacterial soft rot occured at the density of Erwinia $5.9{\sim}6.6{\times}10^5/dry$ soil 1 gram. The density of microbes on continuous fields were higher than that of rotating fields, but there were no significant difference between treated fungicide plot and non treated in the density of microbes, also no difference between Chinese cabbage and radish growing fields.

• Proceedings of the Korean Society of Soil and Groundwater Environment Conference
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• 2003.09a
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• pp.334-337
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• 2003

This study was carried out to investigate the effect of soil properties, such as soil organic matter(SOM) content and water content on die-off and regrowth of indigenous microbes due to in-situ ozonation. Four different soils were collected and the soil samples applied to different ozonation time(0-360 min) were incubated during 4 weeks. Population of the indigenous microbes was monitored during incubation period. The number of indigenous microbes in all samples dramatically decreased (more than 90%) within 30 minutes of ozone injection. With increased ozonation time by 360 minutes, the number of the indigenous microbes decreased by 99.99% in all samples. Die-off of the indigenous microbes due to ozone treatment was inversely proportional to SOM and water content. Especially, sample 3 and Sample 4 containing relatively high SOM content and water content showed high regrowth rate, and this resulted from the increase of water soluble and biodegradable organic fraction in soil water after ozone treatment. Soil sample ozonated for 360 minutes showed minor increase in microbial population during 4 weeks of incubation period.

• Proceedings of the Korean Society of Soil and Groundwater Environment Conference
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• 2002.04a
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• pp.22-25
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• 2002

The effect of in-situ chemical oxidation on the indigenous soil microorganisms (total microbes and PAH-degrading microbes) and contaminant removal were investigated. Field soil contaminated with diesel in gas station was collected and the soil was treated from 0 to 900 minutes by in-situ ozonation as chemical remediation. The treated soil samples were incubated with supplying oxygen during the 9 weeks to understand the characteristics of microbes regrowth, damaged by ozone. The sharp decrease of aromatic fraction and TPH was observed within 60 minutes of ozone application and aromatic fraction and TPH then slowly decreased. The phenanthren-degrading bacteria were the most sensitive to ozonation, because 1 hour of ozonation reduced the microbes from 10$^{6}$ CFU/g-soil to below detection limits.

• Journal of Environmental Health Sciences
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• v.33 no.5
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• pp.462-469
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• 2007

Isolation and application of oil-degradation microbes from the oil-contaminated soil and the determination of optimal operation conditions about the peat moss, the addition for the oil-biodegradation. After all experiments, we have acquired three important conclusions: First, we found out the 4 microbes, Pseudomonas fluorescens, Pseudomonas aeruinosa, Kurtia sp., Bacillus ceres, with excellent capability for the oil-degradation; Second, the optimal operating conditions of the peat moss for TPH treatment were pH $7{\sim}8$, temperature $25{\sim}30^{\circ}C$, water content 20%, mixing 2 times/ day, addition volume 2%; Third, in case of the application to the oil-contaminated soil with 4 mixed microbes, the removal efficiency of TPH was increased from 54% to 83% in oil-contaminated soil and from 65% to 85% in oil-contaminated soil with the peat moss.

• Journal of The Korean Society of Agricultural Engineers
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• v.57 no.3
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• pp.1-7
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• 2015

The objective of this study was to compare fecal microbes survival in soil between compost surface application and soil incorporation. The survival experiment was conducted in six styrofoam beds ($510{\times}325{\times}305(mm)$ in size) filled with sandy loam soil. A half of six boxes were received by compost surface application, while the other half were treated with compost-soil mixture. Duplicated surface and surbsurface soil (20 cm depth) samples were collected at various interval up to 50 days and analyzed for the determination of fecal coliforms and E. coli numbers. As expected, surface applied beds demonstrated two to three magnitudes order greater in both the study microorganisms as compared to soil incorporated beds. Microbial inactivation rate of soil surface was twice as great as subsurface soil condition probably due to exposure to sun light and environmental conditions including moisture loss. When rainfall occurred, microbes on the surface were transported into soil along with water movement. It was concluded that surface compost application may be easier to apply but pose higher risk of human exposure to microbes. Winter compost application may be favorable in alleviating health risk by giving some time for inactivation compared to spring application.

• Toxicological Research
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• v.27 no.4
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• pp.241-246
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• 2011

To obtain soil microorganisms producing antimigratory activity which is important in controlling the metastasis of cancer cells, more than three hundreds of soil microbes were isolated from sixteen soil sources including Namsan mountain and designated as DGU1001-10338. At first, their antibiotic activities were examined by paper-disc method. More than 40 soil microbes produced compounds with antibiotic activity. Then, antimigratory activities of selected soil microorganisms were examined in a sphingosylphosphorylcholine-induced migration assay in PANC-1 cells. Six of 42 soil microorganisms having antibacterial activity also had more than 45% inhibitory activity on migration of PANC-1 cells. These results suggested that selected soil microorganisms were a useful starting point to find compounds for controlling metastasis of cancer cells.

• Journal of Soil and Groundwater Environment
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• v.21 no.5
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• pp.8-15
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• 2016

Bioremediation, which uses microbes to degrade most organic pollutants in soil and groundwater, can be used in solving environmental issues in various polluted sites. In this research, a wind-driven bioventing system is built to degrade about 20,000 mg/kg of high concentration diesel pollutants in soil-pollution mode. The wind-driven bioventing test was proceeded by the bioaugmentation method, and the indigenous microbes used were Bacillus cereus, Achromobacter xylosoxidans, and Pseudomonas putida. The phenomenon of two-stage diesel degradation of different rates was noted in the test. In order to interpret the results of the mode test, three microbes were used to degrade diesel pollutants of same high concentration in separated aerated batch-mixing vessels. The data derived thereof was input into the Haldane equation and calculated by non-linear regression analysis and trial-and-error methods to establish the kinetic parameters of these three microbes in bioventing diesel degradation. The results show that in the derivation of μ_m (maximum specific growth rate) in biodegradation kinetics parameters, K_s (half-saturation constant) for diesel substance affinity, and K_i (inhibition coefficient) for the adaptability of high concentration diesel degradation. The K_s is the lowest in the trend of the first stage degradation of Bacillus cereus in a high diesel concentration, whereas K_i is the highest, denoting that Bacillus cereus has the best adaptability in a high diesel concentration and is the most efficient in diesel substance affinity. All three microbes have a degradation rate of over 50% with regards to Pristane and Phytane, which are branched alkanes and the most important biological markers.

• Korean Journal of Microbiology
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• v.6 no.4
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• pp.131-140
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• 1968

The soil of the reclaimed tidal land, located in Chogi-ri, Is. Kanghwa, Korea was used in this experiment. The experimented soil samples were collected from 18 sites with its time elapsed after the shore-protection works, soil-depth and the vegetation of saline plants, and at each site samplings were conducted monthly from March through October, 1968, for the purposes of examining the changes of microbial populations for the microbes such as bacteria, actinomycetes and fungi, by using the dilution plate method. The numbers of the microbes in these soils generally showed lower levels comparing with those of other soils. The more time elapsed after the reclamation, the higher numbers of the microbes inhibited the soils. Higher populations were there in the surface soils than in the lower part of the area. The surface soils included comparatively better conditions in aeration and contents of organic matter than in the lower part, and this fact was. same as in general soils. However, not so was this in the case of March, April and October due to the higher soil temperatures in the lows. At the experimental sites where the halophytes such as Salicorniu were grown vigourously, the more densly the plants grew, the higher populations of actinomycetes and fungi were, but not in the case of bacterial population. This means, in this soil with dense Salicornia, it is difficult to obtain good-natured soils in short time without a higher population of bacteria. For the rapid utilization of the land soil, in this view of point, the methods increasing the number of bacteria in the soil are needed as well as the cultivation and harvesting Salicorniu which indicated in the privious paper(Hong, et al., 1969a). According to the results of this experiment, the changes of soil-microbial populations in the reclaimed tidal land soil containing high salinity depend deeply upon the interrelations of many environmental factors such as soil-salinity, soil-components and contents, concentration of organic matters, pH, aeration, and air and soil temperatures, as in the general soils.

• Journal of Soil and Groundwater Environment
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• v.14 no.5
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• pp.10-17
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• 2009

Bioremediation has been applied as a proven technology in remediation of TPH contaminated soil. However, the efficiency of biodegradation is dependent on temperature as microbial activity is depressed at lower temperature ranges ($30^{\circ}C{\sim}80^{\circ}C$). The objective of this study was to develop microbes with enhanced activities at the stated temperature conditions and to evaluate the remediation effectiveness of these microbes in TPH contaminated soil. Experiments were conducted to isolate hydrocarbon degradable microbial consortia cultured under different temperature conditions. It was found that there were 5 strains of mesophilic ($30^{\circ}C$) and 3 strains of psychrophilic ($80^{\circ}C$) microbes. The TPH concentration was reduced from 4,044 mg/kg to 1,084 mg/kg, (73.2%) in 10 days by using mesophilic microbial consortia and from 5,427 mg/kg to 1,756 (67.6%) in 50 days with psychrophilic microbial consortia in laboratory cultures under controlled conditions. This rate determination excluded physical degradation such as venting and dilution. A field study was then performed to examine the feasibility of applying these microbes in the land-farming process. In this case, 87.1% of the 2,560 mg/kg TPH contaminated soil was degraded in 56 days. The biodegradation rate coefficient (k) was $0.0374\;day^{-1}$. Findings of this study provide viable options for applying microbes for bioremediation of TPH in lower temperature conditions.

• Journal of Environmental Health Sciences
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• v.30 no.2
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• pp.108-114
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• 2004

Observation of microorganisms collected from contaminated soils has been mainly conducted by using microscopy. Microscopic measurement is occupied an important part of the microorganism experiment, and is used as an important tool to count microorganisms as well as to observe cellular form and mode of life in the field of soil microbe observation. In general, observation equipments for soil microbes consist of electron microscope, camera, frame grabber (image acquisition baud), and image analysis software. Because image analysis software should be linked with frame grabber most equipments have to be imported as the package form. Therefore, the observation system is very expensive and difficult to be operated. In this study, soil microbes＇ observation equipment with the vision system which is easy operated and cheaper than imported one was developed and tested. The efficiency of image capturing and data aquisition with developed frame grabber and software in this experiment was good enough to analyze the image of soil microorganism.