• Korean Journal of Medicinal Crop Science
• /
• v.9 no.4
• /
• pp.310-317
• /
• 2001

The regenerated-bulblets placed in liquid free media resulted in good formation of roots and bulblets. On 1/4 MS free medium, roots and bulblets were predominantly induced. The 1/4 MS liquid medium supplemented with plant growth regulators was the best suitable condition for elongation of leaves and roots. Somatic embryos were frequently developed from embryogenic callus in liquid media with 2,4-D 1mg/ l . On free liquid media, the viability of callus reduced. As the salt strength of MS media reduces, the viability of callus reduced significantly. However, Leaves were induced from several callus clumps. When leaves, roots and bulb-scale segments were placed on MS media containing NAA 1mg/ l or 2,4-D 1mg/ l and various sucrose concentration, the best result about the differentiation, growth of leaf and the differentiation of leaf was obtained on MS media added 1.5% sucrose and 2,4-D 1mg/ l, 3% sucrose and NAA 1mg/ l, and 1.5% sucrose and NAA 1mg/ l, respectively. Also the better result differentiation, growth of root and differentiation of bulb was obtained on MS media with 6% sucrose and NAA 1mg/ l. Spermidine promoted the growth of leaf and the differentiation of bulb. However, spermine promoted the differentiation of leaf, the differentiation and the growth of root in MS solid media. On the MS liquid media, both spermine and spermidine stimulated organogenesis from bulb-scale segments. Regenerated plantlets were acclimatizated and grown in greenhouse in vermiculite + perlite (1 : 1 by volume) well. The optimal soil condition of rooting for plantlets regenerated was in peat moss.

• Applied Biological Chemistry
• /
• v.14 no.1
• /
• pp.9-18
• /
• 1971

In order to obtain basic information on the production of single cell protein from petroleum, more than 400 yeast strains were isolated from various soil samples in Korea utilizing petroleum hydrocarbon as the sole carbon source. A yeast strain showing the highest cell yield among the isolated strains was selected and identified. The optimal culture condition was searched in the flasks shaken throughout the procedure. And the growing characteristics for the selected yeast strain and chemical analysis of the yeast cell component were carried out. The results obtained were as follows: 1. The selected yeast strain was identified as Candida curvata and we named it Candida curvata-SNU 70. 2. The composition of the medium proposed for the present yeast strain is: Light Gas Oil 30ml, Urea 400mg, Ammonium sulfate 100mg, Potasium phosphate (monobasic) 670mg, Sodium phosphate (dibasic) 330mg, Magnesium sulfate 500mg, Calcium carbonate 3g, Yeast extract 50mg, Tween 20 0.05ml, Tap water 1,000ml. 3. Other culture conditions employed for the yeast were pH 5.5-7.0, temp. $30^{\circ}C$ under an affluent aerobic state. 4. Addition of light gas oil in portions to the culture media as the growth proceeded was more effective, especially in the cultivation on the higher oil concentration media. 5. Studies on the propagation of the yeast cells in the light gas oil medium revealed that the yeast has the lag phase lasted 16 hours and the logarithmic growth phase covered 16 to 28 hours. The specific growth rate was about $0.22\;hr^{-1}$ and doubling time was 3.2 hrs. during the logarithmic growth phase. 6. Under the cultural condition employed, the cell yield against the amount of light gas oil (wt%) was 16.1% and the protein content of the dried yeast cells was 48.4%.

• Journal of The Korean Society of Grassland and Forage Science
• /
• v.25 no.4
• /
• pp.251-258
• /
• 2005

This study was conducted to examine the effect of application of pig manure slurry(PMS) for rye cultivation in Paddy-land on rice productivity The field experiments were carried out from 1999 to 2002 on well-grown rice in Paddy-land at Kimje, Chunbuk province in Korea. PMS was manufactured by the fermentation for 6 months in natural condition. Amount of PMS was adjusted equal to total nitrogen value relative to its value of commercial chemical fertilizers. The amount of PMS were spread evenly over the soil surface two times; first in the autumn (before sowing, end of November) and second in the spring(regrowth of rye, middle of March). The field plots were consisted of four kinds (PMS $100\%$ treatment, PMS $200\%$ treatment PMS $100\%$ treatment with half of CF (CF $50\%$), Full of CF treatment (CF $100\%$). The experiment was designed as a randomized complete block. Each treatment was replicated three times. Rices are customarily gown under tropical korean condition as annuals. The results obtained from this study were summarized as fellows. Panicle length of rice was shorter in PMS $100\%$ than other treatments. Ripening grain ratio was the lower in CF treatment. In treatment of PMS $100\%$ and PMS $200\%$, 1000 grain weight was a downward trend as compared to that of full of CF treatments, whereas number of grains per $m^2$ was increased as compared to other treatments. Number of panicles reveals that there is an increase in order; CF $50\% + PMS\;100\%>\;PMS\;100\%\;>\;CF\;100\%\;>\;PMS\;200\%$. Yield of milled rice showed hi인 in CF $100\%$ and $CF 50\%+ PMS 100\%$, but were was no significant difference among treatments. There were did not a regular tendency in the ratio of leaf sheath bright, however the ratio of lodging area was higher in $CF50\%+PMS\;100\%$ treatment than other treatments. Based on the results of this experiment, $CF 50\%+PMS\;100\%$ may be the most effective in rice productivity under application of PMS for rye cultivation in Paddy-land.

• Journal of Korean Society of Environmental Engineers
• /
• v.35 no.8
• /
• pp.554-563
• /
• 2013

The purpose of this study is to evaluate the possibility of nutrient release at up and downstream of Kangchun weir, upstream of Yuju and Ipo weir in Namhan River. For this survey, we measured basic characteristics of the sediments (water content, ignition loss, TOC, TP, SRP, TN, phosphorus fractionation) and conducted nutrients release experiments under both aerobic and anaerobic condition. The overlying water from the sediment-water column was analyzed for nutrients (i.e. TP, $PO_4$-P, TN, $NO_3$-N, $NH_3$-N) everyday for 18days. Result of soil texture experiment showed that sediments are Sand. SRP concentration before the release experiment was different with the value after the release experiment. According to this result, we can find that there were more activated release processes in anaerobic condition. $PO_4$-P increased from 1 to 8 days and remained at the maximum value (7~8 days) afterward. The rapidly increase of $PO_4$-P was observed from 1 to 2~3 days whereas the TP continuously increase from 1 to 18 days. The $PO_4$-P release rate calculated by up to 7~8 days data highly correlated with initial SRP concentration with $R^2$=0.8502. $NO_3$-N release rate appears constantly decreasing trend as -5.7~-3.08 $mg/m^2{\cdot}day$, otherwise the $NH_3$-N release rate, by-product of a organic matter decomposition using nitrate as electron acceptor, was 0.57~2.41 $mg/m^2{\cdot}day$. Substantial portion in TN can be induced by organic nitrogen which originated from the tributary passing through non-point pollutant source. Compared with other similar researches, phosphorus and nitrogen release rates obtained in this study can be considered as relatively low values. Since this study targeted the sediments accumulated by one time of flooding season, there are limitation to generalize theses results. Therefore, it is necessary to consistently monitor and investigate the accumulation of nutrients in the sediment for understanding the effect of weir construction on the overlying water quality.

• Journal of Korean Society of Forest Science
• /
• v.11 no.1
• /
• pp.1-24
• /
• 1970

This experiment was carried out to elucidate the behavior of nutrition absorption and the acid tolerance, on varying soil pH levels, of Pinus thunbergii, Pinus densiflora, Pinus rigida, PInus koraiensis, Larix leptolepis and Abies holophylla which are 6 month old seedlings through examinations of the absorption behavior, the acid and alkali tolerance of N,P,K, Ca and Fe in the sand culture media with artificially controlled pH. 1. The increase rate of dry matter conditioned by the behavior of nutrition absorption in a strong acidic condition (pH3) was found in the following decreasing order of Pinus rigida, Pinus thunbergii. This order, therefore, can also be said to apply to the case of acid tolerance. 2. The increase rate of dry matter in a strong alkaline condition (pH9) was found in the following decreasing order of Pinus thunbergii, Pinus rigida, Pinus densiflora, Pinus koraiensis, Abies hoplohylla, Larix leptolepis. This order, therefore can be said to apply to the case of alkali tolerance. 3. Considering the absorption behavior of K and Ca, it is possible to decide the tolerance of acid from the absorption behavior and it can be said that if the absorption quantity is K>Ca the acid tolerance is strong and vice versa. 4. Considering the ratio of K:Fe and Ca:Fe in this experiment, a strong acid tolerance shows a higher ratio of K/Fe and vice versa. And also a strong acid tolerance shows a lower ratio of Ca/Fe and vice versa. It is therefore possible to decide the acid tolerance from the absorption behavior of K, Ca and Fe. 5. Of the tree species subjected to this experiment, Pinus thunbergii was the strongest in both acid and alkali tolerance. 6. In spite of that in all species the dry weight in the root is smailer than that in the top, the quantity of Fe contained in the root was larger than that in the top.

• Korean Journal of Plant Resources
• /
• v.18 no.1
• /
• pp.1-7
• /
• 2005

This study was conducted to investigate the possibility of cultivating tea plants planted in the mountain area of west Gyeongnam province, Korea for 2 years from March 27th, 2001 to July 30th 2003. Ninety each plants collected from 5 different sife were cultivated in nursery cup pot$(\phi\;16cm)$. in the greenhouse condition and transplanted in 5 different location and monitored their survival growing state etc. The results obtained are as follow : 1. Survival rate tea plants after transfer to soil was relatively high Gaya-myeon Hanyang-gun with $90.0\%$ and the lowest in Buksang-meyon Geochang-gun with $80.0\%$. The tea plant collected from Gaya-myeon showed the best growing activity at early stage. 2. For the second year of harvesting time, survival rate was the highest in Machun-myeon Hamyang-gun with $92.2\%$ and the lowest was Buksang-myeon Geochang-gun with $76.7\%$. 3. For the 3rd year of harvesting time, it was impossible to data collection because of the most upper parts of plants were killed by severe freezing weather condition. In the Baekjeon-myeon, Hamyang-gun Buksang-myeon, Ungyang-myeon Geochang-gun, which are severly cold$(below\;-10^{\circ}C)$ in winter season, seems not a suitable places for tea plant cultivation since it is very different to harvest the young loaves in growing season. In conclnsion we could select two sites Gaya-myeon, Hapcheon-gun, Machun-myeon, Hamyang-gun, as tea plant cultivation in the mountain area of west-Gyeongnam province, korea.

• Journal of the Korean earth science society
• /
• v.39 no.5
• /
• pp.419-435
• /
• 2018

The purpose of this study is to understand meteorological and climatological factors that have influence on the garlic product in Seosan and Taean, and to analyze the economic value according to the use of climatical information data for garlic farmers. The climatological characteristics and trends in this area are analyzed using the meteorological data at the Seosan local meteorological agency from 1984 to 2013, the national statistical data for the product of garlic from 1989 to 2013, and the scenario data for climate change (RCP 4.5 and 8.5) for the period from 2001 to 2100. The results are as follows. First, the condition of lower temperature for garlic growth in winter season is satisfied with the mean air temperature. The wind speed are lower and stronger in Seosan and Taean than other garlic area. The suitable condition for the growth of northern type of garlic shows the decreasing trend in the accumulated precipitation in May. However, the area of growing the northern type garlic in the future is likely diminished because mean air temperature, accumulated precipitation, and mean wind speed are strong in the harvest time of garlic. Second, the seedtime of the northern and southern type of garlic using climate change scenarios (RCP 4.5, 8.5) in Seosan and Taean is getting late as time passes. and the harvest time gets faster, which indicates s that the period of garlic cultivation becomes shorter from 50 days to around 90 in the next 100 years. Third, the beginning days of white rot and delia platura of garlic are estimated by applying to the meteorological algorithm using mean air temperature and soil humidity. Especially, the beginning day of white rot garlic is shown to be faster according to the scenarios of RCP 4.5 and RCP 8.5. Fourth, the product of garlic (kg/10a) shows a high correlation with the minimum air temperature of a wintering time, the mean wind speed of a wintering time, the accumulated precipitation of a corpulent time, and the mean relative humidity of corpulent time of garlic. On the other hand, the analysis of garlic product when using the meteorological information data in cultivating garlic in Seosan and Taean reveals that the economic value increases up to 9% in total.

• Applied Biological Chemistry
• /
• v.16 no.1
• /
• pp.1-11
• /
• 1973

In order to obtain the basic informations on the production of single cell protein from ethanol, 145 yeast strains utilizing ethanol as a sole carbon source were isolated from 32 soil samples in Korea. A yeast strain showing the highest cell yield among the isolated strains was selected and identified. The optimum culture condition, utilization of other carbon sources and the cultural characteristics for the selected yeast, and the chemical analysis of the yeast cell composition, and utilization of ethanol by the selected yeast were investigated. All the culture was carried out in the shaking flasks. The results obtained were as follows: 1. The selected yeast strain was identified as Debaryomyces nicotianae-SNU 72. 2. The optimum composition of the medium for the selected yeast is : Ethanol 40 ml, Urea 0.5 g, Potassium phosphate (dibasic) 0.5 g, Ammoium phosphate (monobasic) 0.15 g, Magnesium sulfate 0.05 g, Calcium chloride 0.01g, Yeast extract 0.005 g, Tap water 1000 ml. 3. The optimum pH was 5.0-5.5, the optimum temperature $30-33^{\circ}C$ and the aerobic state was unimportant. 4. Utilization of methanol, n-propanol, iso-propanol, n-butanol, iso-butanol, tert-amyl alcohol and acetic acid by the selected yeast was very weak. So substitution of the subtrate was thought to be impossible. 5. Studies on the propagation of the yeast cells showed that the lag phase of the yeast cells lasted 16 hours, and the logarithmic growth phase extended 16 to 28 hours. The specific growth rate was about $0.19\;hr^{-1}$ and the doubling time was 3.6 hours during the logarithmic growth phase. 6. As the result of the chemical analysis of the dry yeast cells, the content rate of the crude protein was 55.19 %, the content of others was similar to the average content of the yeast component. 7. After 34 hours cultivation, under the optimum culture condition investigated, the dry cell yield against the amount of the added ethanol was 53.4 % (W/V%), the dry cell yield against the amount of the utilized ethanol was 73.6 % (W/V%), the evaporation rate of ethanol was about 19.1 %.

• Journal of Korea Water Resources Association
• /
• v.51 no.10
• /
• pp.905-916
• /
• 2018

The purpose of this study is to evaluate the climate change impact on watershed hydrology and flow duration in Geum River basin ($9,645.5km^2$) especially by extreme scenarios. The rainfall related extreme index, STARDEX (STAtistical and Regional dynamical Downscaling of EXtremes) was adopted to select the future extreme scenario from the 10 GCMs with RCP 8.5 scenarios by four projection periods (Historical: 1975~2005, 2020s: 2011~2040, 2050s: 2041~2070, 2080s: 2071~2100). As a result, the 5 scenarios of wet (CESM1-BGC and HadGEM2-ES), normal (MPI-ESM-MR), and dry (INM-CM4 and FGOALS-s2) were selected and applied to SWAT (Soil and Water Assessment Tool) hydrological model. The wet scenarios showed big differences comparing with the normal scenario in 2080s period. The 2080s evapotranspiration (ET) of wet scenarios varied from -3.2 to +3.1 mm, the 2080s total runoff (TR) varied from +5.5 to +128.4 mm. The dry scenarios showed big differences comparing with the normal scenario in 2020s period. The 2020s ET for dry scenarios varied from -16.8 to -13.3 mm and the TR varied from -264.0 to -132.3 mm respectively. For the flow duration change, the CFR (coefficient of flow regime, Q10/Q355) was altered from +4.2 to +10.5 for 2080s wet scenarios and from +1.7 to +2.6 for 2020s dry scenarios. As a result of the flow duration analysis according to the change of the hydrological factors of the Geum River basin applying the extreme climate change scenario, INM-CM4 showed suitable scenario to show extreme dry condition and FGOALS-s2 showed suitable scenario for the analysis of the drought condition with large flow duration variability. HadGEM2-ES was evaluated as a scenario that can be used for maximum flow analysis because the flow duration variability was small and CESM1-BGC was evaluated as a scenario that can be applied to the case of extreme flood analysis with large flow duration variability.

• Journal of Sericultural and Entomological Science
• /
• v.49 no.2
• /
• pp.51-55
• /
• 2007

Investigation of atmospheric data and rearing results was conducted to analyze the effects of atmospheric factors such as temperature and precipitation on silkworm in 8 rearing places in which local adaptation test was being conducted with different mulberry growth condition, soil and atmosphere during spring and autumn rearing season of 2006. The atmospheric characteristics of spring rearing time are as follows. The average temperatures of young silkworm, old silkworm, mounting were $17.7^{\circ}C$, $19.8^{\circ}C$, $21.5^{\circ}C$ respectively, and $1^{\circ}C$ higher than normal year. The precipitation of young silkworm, old silkworm, mounting were 15.1 mm, 6.9 mm, 7.0 mm, respectively, and 22.9 mm lower than normal year in old silkworm and mounting. The daylight hours in larval stage was 1.7 hour shorter than normal year, but no difference in mounting. Thus precipitation was lower and temperature was higher than normal year in 2006. The rearing results of 2006 were 1 kg lower than normal year in cocoon yields per 10,000 3 rd molted larvae, single cocoon weight and cocoon shell percentage were a little higher. The atmospheric characteristics of spring rearing season are as follows. The average temperatures of young silkworm, old silkworm, mounting were $25.1^{\circ}C$, $20.5^{\circ}C$ and $19.9^{\circ}C$ respectively, temperature in young silkworm was $1^{\circ}C$ higher than normal year, and temperature in old silkworm was $1.3^{\circ}C$ lower than normal year. The precipitation of young silkworm, old silkworm, mounting were 110.2 mm, 4.6 mm, 3.7 mm, respectively and there were little differences compared to normal year. The atmospheric condition of 2006 which was similar to normal year did not affect the autumn rearing results of 2006. Namely, the single cocoon weight and cocoon shell weight were not different from normal year, and the same was cocoon shell percentage.