• Journal of Microbiology and Biotechnology
• /
• v.13 no.3
• /
• pp.463-468
• /
• 2003

Polychlorinated biphenyl are toxic pollutants and their degradation is quite slow in the environment. Recently, interest if bioremediation using PCB-degrading bacteria has increaset,. In a previous report, plant terpenes (p-cymene, (S)-(-)-limonene, ${\alpha}-pynene$, and ${\alpha}-terpinene$) have been found to be utilized by a PCB degrader and to induce the biphenyl dioxygenase gene in pure culture. In this study, Pseudomonas pseudoalcaligenes KF707, a PCB-degrading Gram-negative soil bacterium, was used to determine whether the terpene stimulation of PCB degrader occurred in the natural environment. First, P. pseudoalcaligenes KF707 was genetically tagged using a transposon with gfp (green fluorescent protein) as a reporter gone. The population dynamics of P. pseudoalcaligenes KF707 harboring gfp gene in a PCB-contaminated environment was examined with or without terpenoids added to the microcosm. About 10-100-fold increase was found in the population of PCB degraders when terpene was added, compared with control (non-terpenes samples and biphenyl added samples). It was proposed that the gfp-monitoring system is very useful and terpenes enhance the survivability of PCB degraders in PCB-contaminated environments.

• Journal of Korean Tunnelling and Underground Space Association
• /
• v.9 no.4
• /
• pp.361-375
• /
• 2007

This paper presents the results of investigation on tunnelling-induced ground surface settlement characteristics in water bearing ground using finite element (FE) stress-pore pressure coupled analysis. Fundamental interaction mechanism of ground and groundwater lowering was first examined and a number of influencing factors on the results of the coupled FE analysis were identified. A parametric study was then conducted on the influencing factors such as rock type, thickness of soil layer, permeability of shotcrete lining, among others. The results indicate that the tunneling-induced groundwater drawdown results in a deeper and wider settlement trough than without groundwater drawdown, and that the Error function approach does not yield satisfactory result in predicting a settlement profile. The results of analysis are summarized so that the relationship between the settlement and the influencing factors can be identified.

• Proceedings of the Korean Geotechical Society Conference
• /
• 2008.03a
• /
• pp.955-962
• /
• 2008

This study concerns the effect of drainage in reinforced soil on its stability during rainfall. A series of finite-element analysis based transient seepage analysis were performed on a number of cases with different drainage conditions in terms of layers of geotextiles installed in the reinforced zone. The results were then coupled with the limit-equilibrium slope stability analysis to investigate the variation of global stability factor of safety with rainfall infiltration into the reinforced wall. The results were thoroughly analyzed to get insight into the mechanism of pore water pressure reduction effect of the geotextile and into its effect on overall slope stability. It is shown that layers of geotextile installed in the reinforced zone can prevent decrease in suction in the reinforced zone during rainfall, thereby reducing potential risk of decreasing shear strength of the reinforced zone. Practical implications of the findings were discussed.

• Proceedings of the Korean Geotechical Society Conference
• /
• 2010.03a
• /
• pp.877-882
• /
• 2010

This paper is a result of investigating causes and main characteristics of landslides, occurred at Hongcheon area in Gangwondo during July in 2006, by collecting relevant data and visiting site. The main cause of landslides in this area has been found to be saturation of the ground wetted by a series of precipitations during 10~13 July and the heavy rainfall during 15 July. The pattern of the landslides could be classified as translational failure, occurred at the boundary between the relatively thin weathered residual soil and the mother rock. By analyzing a number of failed slopes based on site visit and reviewing collected data, typical widths of failed slopes are in the range of 10~20m (minimum: 5m, maximum: 70m). Lengths of landslide area are in the wide range of 10~450m. Most of area are less than 20m in width and 100m in length so that their shapes are long and narrow, frequently observed in Korea, and their areas are relatively small size of around $1000m^2$. The inclinations of the failed slopes are in the range of $10{\sim}60^{\circ}$ while the most probable slope angle is about $20{\sim}25^{\circ}$.

• Geomechanics and Engineering
• /
• v.14 no.3
• /
• pp.233-240
• /
• 2018

Pullout tests are usually employed to determine the ultimate bearing capacity of reinforced soil, and the load-displacement curve can be obtained easily. This paper presents an analytical solution for predicting the full-range mechanical behavior of a buried planar reinforcement subjected to pullout based on a bi-linear bond-slip model. The full-range behavior consists of three consecutive stages: elastic stage, elastic-plastic stage and debonding stage. For each stage, closed-form solutions for the load-displacement relationship, the interfacial slip distribution, the interfacial shear stress distribution and the axial stress distribution along the planar reinforcement were derived. The ultimate load and the effective bond length were also obtained. Then the analytical model was calibrated and validated against three pullout experimental tests. The predicted load-displacement curves as well as the internal displacement distribution are in closed agreement with test results. Moreover, a parametric study on the effect of anchorage length, reinforcement axial stiffness, interfacial shear stiffness and interfacial shear strength is also presented, providing insights into the pullout behaviour of planar reinforcements of MSE structures.

• Biotechnology and Bioprocess Engineering:BBE
• /
• v.3 no.1
• /
• pp.44-47
• /
• 1998

Maximum cellulase production was sought by comparing the activities of the cellulases produced by different Trichoderma reesei strains and Aspergillus niger. Trichoderma reesei Rut-C30 showed higher cellulase activity than other Trichoderma reesei stains and Aspergillus niger that was isolated from soil. By optimizing the cultivation conditions during shake flask culture, higher cellulase production could be achieved. The FP(filter paper) activity of 3.7U/ml and CMCase (Carboxymethylcellulase) activity of 60U/ml were obtained from shake flask culture. When it was grown in 2.5L fermentor, where pH and DO levels are controlled, the enzyme activities were 133.35U/ml (CMCase) and 11.67U/ml(FP), respectively. Ammonium sulfate precipitation method was used to recover enzymes from fermentation broth. The dried cellulase powder showed 3074.9U/g of CMCase activity and 166.7U/g of FP activity with 83.5% CMCase recovery.

• Structural Engineering and Mechanics
• /
• v.73 no.5
• /
• pp.599-612
• /
• 2020

A combination of a gravity wall and an anchor beam is widely used to support the high soil deposit on rock mass. In this study, two groups of shaking table test were performed to investigate the responses of such combined retaining structure, where the rock masses were shaped with a flat surface and a curved surface, respectively. Meanwhile, the dynamic numerical analysis was carried out for a comparison or an extensive study. The results were studied and compared between the combined retaining structures with different shaped rock masses with regard to the acceleration response, the earth pressure response, and the axial anchor force. The acceleration response is not significantly influenced by the surface shape of rock mass. The earth pressure response on the combined retaining structure with a flat rock surface is more intensive than the one with a curved rock surface. The anchor force is significantly enlarged by seismic excitation with a main earthquake-induced increment at the first intensive pulse of Wenchuan motion. The value of anchor force in the combined retaining structure with a flat rock surface is generally larger than the one with a curved rock surface. Generally, the combined retaining structure with a curved rock surface presents a better seismic performance.

• Korean Journal of Remote Sensing
• /
• v.23 no.3
• /
• pp.153-160
• /
• 2007

Forest cover density studies using high resolution satellite data and object oriented classification are limited in India. This article focuses on the potential use of QuickBird satellite data and object oriented classification in forest density mapping. In this study, the high-resolution satellite data was classified based on NDVI/pixel based and object oriented classification methods and results were compared. The QuickBird satellite data was found to be suitable in forest density mapping. Object oriented classification was superior than the NDVI/pixel based classification. The Object oriented classification method classified all the density classes of forest (dense, open, degraded and bare soil) with higher producer and user accuracies and with more kappa statistics value compared to pixel based method. The overall classification accuracy and Kappa statistics values of the object oriented classification were 83.33% and 0.77 respectively, which were higher than the pixel based classification (68%, 0.56 respectively). According to the Z statistics, the results of these two classifications were significantly different at 95% confidence level.

• Journal of Species Research
• /
• v.11 no.4
• /
• pp.310-320
• /
• 2022

As a subset study to discover indigenous prokaryotic species in Korea, we isolated 20 bacterial strains and assigned them to the phyla Actinobacteria, Bacteroidota, Firmicutes, and Proteobacteria. From the high 16S rRNA gene sequence similarity (≥98.7%) and formation of a robust phylogenetic clades, we determined that each strain belonged to independent, predefined bacterial species. There are no official reports of these 20 species in Korea; therefore, 7 strains of the Actinobacteria, 2 strain of the Bacteroidota, 3 strains of the Firmicutes, and 8 strains of the Firmicutes are described in Korea for the first time. Gram reaction, colony and cell morphology, basic biochemical characteristics, and isolation sources are also described in the species description section.

• Proceedings of the Plant Resources Society of Korea Conference
• /
• 2020.12a
• /
• pp.79-79
• /
• 2020

Halophyte is a plant that has evolved to grow well in salty places, and is mainly distributed in coastal wetlands, sand dunes, salt fields, and reclaimed lands in tidal flats and river estuaries. Because it grows in the soil where seawater enters and exits, it is very abound in natural minerals and produces certain metabolites to survive sustainably in the surrounding environment. In this study, anti-inflammatory studies were conducted using 15 kinds of halophyte to secure data on halophytes of infinite value as functional materials. The aim of this study was to select a group of halophytes that regulate iNOS expression, which is involved in the secretion of inflammatory cytokines and nitric oxide production in LPS-induced macrophages. Among the 15 species of halophyte, except for Triglochin maritimum, Suaeda japonica, and Carex pumila, NO production was reduced in 12 species of halophytes, and 7 species of halophyte (Suaeda asparagoides, Artemisia fukudo, Spergularia marina, Aster tripolium, Suaeda australis, Atriplex subcordata, Calystegia soldanella) significantly decreased the expression levels of TNF-α and IL-1β.