• Korean Journal of Microbiology
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• v.44 no.4
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• pp.352-357
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• 2008

We evaluated cultivation methods to obtain pure cultures of previously uncultivated bacteria from soil. Soil bacteria (suspensions) were inoculated onto various oligotrophic media with one of the following additives: 1) soil extract; 2) anthraquinone disulfonate (humic acid analogue); 3) acyl homoserine lactones (quorum-signaling compounds); 4) catalase (for the protection of bacteria from exogenous peroxides). After the relatively long period (60 days) of incubation with elevated concentrations of $CO_2$ (5%, v/v), the media containing catalase showed the highest colony count. We purified 147 randomly selected colonies from the media and the isolates were subjected to the phylogenetic analyses of their 16S rRNA gene sequences. Phylogenetic analysis revealed that approximately 30% of the isolates might belong to novel species or novel family, suggesting that the media and incubation conditions used could be useful for the cultivation of as-yet-uncultured bacteria. Especially, bacteria belonging to the phylum Acidobacteria, ubiquitous bacterial taxon known as an uncultured bacterial group (at least difficult to culture from environmental samples), were successfully cultured in this study.

• Journal of the Korean Geosynthetics Society
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• v.14 no.2
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• pp.71-79
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• 2015

This paper describes vegetation based soil-media hydroseeding measures that have been previously applied as slope revegetation methods show problems such as insufficient binding force, drying, and insufficient organic matter. In particular, in the case of slope faces in regions where scattering is severe, a vicious circle exists in which remarkably low vegetation cover rates and increases in withering rates over time lead to further decreases in vegetation cover rates, which lead to further increases in erosion and scattering. Therefore, in the present study, environment friendly soil stabilizers were applied for resistance against erosion or scattering and engineering evaluations such as long-term immersion tests and flow resistance tests were conducted to determine appropriate mixing ratios. According to the results of long-term immersion tests utilizing environment friendly soil stabilizers and existing greening soil based materials, 100% collapse occurred at 30 hours and 40 days in the case of soil stabilizer mixing ratios of 0% and 2%, respectively. While the original form of the samples remained intact until the experiment was completed in the case of mixing ratios exceeding 4% indicating that 2% or higher soil stabilizer mixing ratios could affect the maintenance of forms even under extreme conditions. In addition, artificial rainfall tests were conducted on 40, 45, and 55 degree slope faces to evaluate the structural stability of vegetation based materials. Flow resistance tests were conducted on soil stabilizer mixing ratios of 0, 4, 8% to evaluate erosion resistance capability. Based on the results of the tests, environment friendly soil stabilizers applied for prevention of scattering or resistance against erosion by rainwater are considered to provide large effects to reduce losses and loss rates showed a tendency of decreasing rapidly when soil stabilizers were mixed.

• Weed & Turfgrass Science
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• v.7 no.1
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• pp.54-61
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• 2018

Zoysiagrass (Zoysia spp.) exists spotly in Indonesia and it has potential to be used in parks, golf courses, and football fields. Many football fields and golf course fairways use sand as top soil over native soil. This study aims to analyze growth and quality of two native zoysiagrasses Zis and Zim. Zis is a native zoysiagrass collected in sea-side and Zim is a native zoysiagrass collected in mountain area. Both types of zoysiagrasses were planted at field with altitude of 300 m with various growing media mixes of sand and reservoir's sediment. Thickness of the growing medium was 10 cm over an alfisol clay soil. Experimental plots were constructed using factorial completely randomized design with two native zoysiagrasses and 5 types of growing media. Two ecotypes of native zoysiagrasses showed different in growth habits combined with mixtures of growth media. Zim showed higher growing speed including more vigor with uniformity and texture than Zis. There were not significanthly differences on leaf color and root length between two ecotypes of native zoysiagrasses collected in Indonesia.

• Horticultural Science & Technology
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• v.33 no.5
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• pp.658-667
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• 2015

This research was conducted to investigate the influence of various levels of fused superphosphate as pre-planting fertilizer on the growth of red-leaf lettuce and changes in the chemical properties of the soil solution in three root media, namely coir-dust plus expanded rice hull (8:2, v/v; CD+ERH), carbonized rice hull (6:4; CD+CRH), or ground and aged pine bark (8:2; CD+GAPB). The amounts of fused superphosphate (FSP) incorporated into the three root media during formulation were controlled from 0 to $6.0g{\cdot}L^{-1}$ in $1.5g{\cdot}L^{-1}$ increments. The root media containing fertilizers were packed into 300 mL plastic pots and seedlings of red-leaf lettuce at the 3rd leaf stage were transplanted. After transplanting, the crops were fed with a solution of neutral fertilizer ($100mg{\cdot}L^{-1}$). The growth of red-leaf lettuce was investigated 5 weeks after transplanting and soil solutions were extracted and analyzed every week for pH, EC, and concentrations of macro-nutrients. The elevation of application rates of FSP in the three root media resulted in better growth, and the crops grown in CD+ERH and CD+GRPB had greater fresh and dry weights than those in CD+CRH when compared among the treatments of equal amounts of FSP. The pH and $PO_4{^{-3}}$ concentrations in the soil solution of CD+CRH at 3 weeks after transplant were in the ranges of 4.0 to 4.8 and 20 to $100mg{\cdot}L^{-1}$, respectively. These were lower pH and higher $PO_4{^{-3}}$ concentrations than those in CD+ERH and CD+GAPB. The $K^+$ concentrations were higher in CD+CRH than those in the other two root media, and the elevation of FSP application rates resulted in higher $Ca^{+2}$, $Mg^{+2}$ and $SO_4{^{-2}}$ concentrations in soil solution of the three root media. The $NO_3$-N concentrations in soil solution rose continuously during crop cultivation, implying that the leaching percentage was elevated. The soil solution EC varied, showing the same tendencies as the $NO_3$-N concentrations. The above results indicated that the CD+ERH and CD+GRPB media performed better than CD+CRH, and optimum application rates of FSP in the three root media were 4.5 to $6.0g{\cdot}L^{-1}$ for pot cultivation of red-leaf lettuce.

• Proceedings of the Computational Structural Engineering Institute Conference
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• 2008.04a
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• pp.14-19
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• 2008

This paper presents dynamic infinite element formulations which have been developed for soil-structure interaction analysis both in frequency and in time domains by the present authors during the past twenty years. Axisymmetric, 2D and 3D layered half-space soil media were considered in the developments. The displacement shape functions of the infinite elements were established using approximate expressions of analytical solutions in frequency domain to represent the characteristics of multiple waves propagating into the unbounded outer domain of the media. The proposed infinite elements were verified using benchmark examples, which showed that the present formulations are very effective for the soil-structure interaction analysis either in frequency or in time domain. Example applications to actual interaction problems are also given to demonstrate the capability and versatility of the present methodology.

• Journal of the Korea Institute of Military Science and Technology
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• v.2 no.1
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• pp.30-37
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• 1999

Transmissivity and the signal loss in soil are computed. An electric field expression for the inhomogeneous complex media modelled by two layers is shown as an integral form. Volume scattering occurs in inhomogeneous media, and iterative Born approximation is used to analyze this scattering effect. The degree of randomness is controlled by specifying the variance and correlation length. Expression for the transmissivity and the signal loss is presented as the parameter of soil moisture contents, soil particle radius, temperature and frequency. The analysis shows that big deviation in signal loss depends on the temperature variation remarkably and the physical reason of unusual level is explained.

• Journal of the Korean Society of Groundwater Environment
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• v.4 no.4
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• pp.223-230
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• 1997

DRASTIC system developed by U.S.EPA, is widely used for assessing regional groundwater pollution susceptibility by using hydrogeological factors such as depth to water, net recharge, aquifer media, soil media, topography, vadose zone media, hydraulic conductivity. The system can be applied to site selection of well or waste disposal and landuse for groundwater protection. In this study, hydrogeological spatial database of Younggwang area about topography, drainage, well, geology, soil and landuse was constructed using GIS (Geographic Information System) and regional groundwater pollution susceptibility is analyzed using the spatial database and GIS overlay technique.

• Journal of the Korean Institute of Landscape Architecture
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• v.27 no.3
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• pp.109-113
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• 1999

The objective of this research was to develop growth media for artificial ground by blending calcined clay and coconut peat. To achieve this, aggregates of clay particles were mixed with disel oil and heated to high temperature(1150~120$0^{\circ}C$) to expand clays. The particle sizes of expanded clay were controlled to 2~5mm in diameter. Then expanded clayes were mixed with coconut peat and changes of soil physicochemical properties and their effect on plant growth of Hedera L. were determined. The infiltration rate of calcined clay was very high, but the water holding capacity, the cation exchange capacity(CEC), and the nutrient contents were low. The characteritics of coconut peat was vice verse to calcined clay. This indicates that the mixture of calcined clay and coconut peat have the better characteristics than each material. As compared to mineral soil, the infiltration rate, the water holding capacity, the CEC and the nutrient contents increased, but bulk density decreased to about 1/4. And, Hedera L. grown in the mixture of calcined clay and coconut peat(6:4, v/v) had higher plant height, longer leaf length, more total number of leaves per plant and fresh weight than that grown in mineral soil, but statistical differences were not observed between two treatments.

• Journal of Korean Society on Water Environment
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• v.22 no.2
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• pp.209-214
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• 2006

The purpose of this study is to improve the stream water quality by the experimental hybrid constructed wetland system. It consisted of the water layer, sand bed planted reeds, irises and roses, gravel bed, yellow-soil media bed and a flow shifter (FS) which can reverse top and bottom flow in the middle of the wetland. The organic compounds and nitrogen removal efficiencies varied with the seasons, namely temperature change. In summer, the mean efficiencies of COD and TN in the outflow from this wetland system were 63.4 and 48.0% and shown the highest, respectively, whereas, the suspended solids and phosphorus removal efficiencies seemed to be less affected by temperature. As a result of inspecting the decreasing trend of pollutants, nitrification-denitrification in the wetland was the major removal mechanism for nitrogen, the nitrogen reduction was especially enhanced by the application of a FS in the wetland, and phosphorus reduction was mainly occurred as a consequence of adsorption of the yellow-soil media.

• Korean Journal of Plant Resources
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• v.28 no.4
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• pp.533-540
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• 2015

Germination properties, leachate electrical conductivity (EC), and inorganic compound leaching were analyzed to ascertain the storage ability and change of physiological characteristics during storage of Hippophae rhamnoides seeds. Seeds were placed in an incubator at 25℃ and sown in different soil media (sand, vermiculite and horticultural substrate) after being stored for 6, 18 and 30 months at 2℃. All germination properties decreased in accordance to an increase of the seed storage period. Compared with the seed storage for 18 months, germination percentage (GP), germination performance index (GPI), and germination value (GV) of seeds stored for 30 months decreased by more than 50%. When the seeds were sown in different soil media in a greenhouse, those germination properties were similar to the seeds germinated in an incubator, and mean germination time, GPI and GV had a significant difference except GP among soil media. EC and inorganic ion concentration had a strong positive correlation with the seed storage period, but the ratios of inorganic ions from stored seeds revealed that K⁺/Mg²⁺ and Na⁺/Mg²⁺ were inversely correlated with the storage period.