• Title/Summary/Keyword: Soil DNA

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A New Record of Neosartorya aureola Isolated from Field Soil in Korea

  • Adhikari, Mahesh;Kim, Sangwoo;Yadav, Dil Raj;Kim, Changmu;Lee, Hyang Burm;Lee, Youn Su
    • The Korean Journal of Mycology
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    • v.43 no.3
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    • pp.191-195
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    • 2015
  • A new species of Neosartorya was recovered during investigation of the fungal community in soil samples collected from different locations in Korea; Neosartorya aureola KNU14-7 was isolated for the first time from field soil in Korea and identified based on the internal transcribed spacer region of rDNA and morphological characteristics. The species has not been officially reported from Korea and we report it here with description and figures.

Sporothrix stylites : A New Record from Field Soil in Korea

  • Park, Sangkyu;Lee, Seung-Yeol;Back, Chang-Gi;Kang, In-Kyu;Ten, Leonid;Lee, Hyang Burm;Jung, Hee-Young
    • The Korean Journal of Mycology
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    • v.45 no.3
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    • pp.224-228
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    • 2017
  • The fungal strain, designated KNU16-008, was isolated from field soil in Chungcheongnam-do, Korea. The isolated fungi was characterized by morphological and phylogenetic analyses. Isolated fungus showed typical morphological characteristics of the genus Sporothrix. Based on its phylogenic analysis using internal transcribed spacer (ITS) of rDNA and ${\beta}$-tubulin gene sequences, the strain KNU16-008 was identified as Sporothrix stylites. This species has not been previously reported in Korea.

Isolation and Characterization of a Dibenzothiophene Degrading Sulfate-Reducing Soil Bacterium

  • Kim, Hae-Yeong;Kim, Tae-Sung;Kim, Byung-Hong
    • Journal of Microbiology and Biotechnology
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    • v.1 no.1
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    • pp.1-5
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    • 1991
  • Sulfate-reducing bacteria have been isolated from soil and their abilities to degrade dibenzothiophene (DBT) were compared with those of type cultures. Among the strains tested a soil isolate M6 showed the highest ability to degrade DBT. Isolate M6 was characterized as a mesophilic obligatory anaerobe. The morphology of the bacterium was vibrioid with the size of $0.4-0.7{\;}\mu\textrm{m}{\;}by{\;}1.0-1.5{\;}\mu\textrm{m}$. Gram reaction was negative and nonsporulating. Desulfoviridin is present. Lactate, pyruvate, ethanol and malate supported growth of the bacterium in the presence of sulfate. Sulfate, sulfite, thiosulfate and sulfur served as electron acceptors for growth. Hydrogenase was present. The mol% of guanine and cytosine of DNA was determined as 56%. The bacterium produced viscous material. From these results, the isolate M6 was identified as Desulfovibrio desulfuricans.

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Correlation between Phylogeny and Zn-Resistance in Methylobacterium Species Isolated from Non-Polluted Soil Environments

  • Kim, Hong-Ik;Kazunori Nakamura;Hiroshi Oyaizu
    • Proceedings of the Korean Society for Applied Microbiology Conference
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    • 2000.10a
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    • pp.118-124
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    • 2000
  • Zn-resistant Methylosobacterium strains were isolated from several non-polluted soil samples collected in all over Japan. Zn-resistant Methylosobacterium strains were predominantly detected in all soil samples and they were also characterized as a UV-tolerant bacteria. The MIC test revealed that the isolates have high zinc resistance in comparison with that of reference Methylosobacterium strains obtained from culture collections. The 16S rDNA-based phylogenetic analysis showed that all strains were divided into two clusters designated as cluster A and cluster B in the present study. All isolates were distributed only in the cluster A. The phylogenetic clustering also well coincided with the differences in the pattern of carbon source utilization.

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First Report of Neopestalotiopsis australis Isolated from Soil in Korea

  • Park, Sangkyu;Lee, Seung-Yeol;Lee, Jae-Jin;Back, Chang-Gi;Ten, Leonid;Lee, Hyang Burm;Jung, Hee-Young
    • The Korean Journal of Mycology
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    • v.44 no.4
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    • pp.360-364
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    • 2016
  • Fungal strain KNU16-005 was isolated from field soil in Jeollabuk-do, Korea. Based on its morphological characteristics and phylogenic analysis using internal transcribed spacer regions of rDNA, ${\beta}$-tubulin and translation elongation factor 1-alpha gene sequences of KNU16-005 were identified as Neopestalotiopsis australis. This species has not been previously reported in Korea.

A New Record of Pseudallescheria boydii Isolated from Crop Field Soil in Korea

  • Babu, A. Giridhar;Kim, Sang Woo;Yadhav, Dil Raj;Adhikari, Mahesh;Kim, Changmu;Lee, Hyang Burm;Lee, Youn Su
    • Mycobiology
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    • v.42 no.4
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    • pp.397-400
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    • 2014
  • Pseudallescheria boydii KNU13-2 was isolated from crop field soil and identified by analysis of internal transcribed spacer regions of rDNA and morphological characteristics. In the literature, P. boydii has been mentioned as a human pathogen. This is the first record of P. boydii isolated from crop field soil in Korea.

First Report of Simplicillium cylindrosporum Isolated from Soil in Korea

  • Park, Sangkyu;Lee, Seung-Yeol;Lee, Jae-Jin;Lee, Hyang Burm;Ten, Leonid;Jung, Hee-Young
    • The Korean Journal of Mycology
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    • v.45 no.2
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    • pp.139-144
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    • 2017
  • The fungal strain KNU16-006 was isolated from field soil in Daegu, Korea. The isolate was identified as Simplicillium cylindrosporum on the basis of its morphological characterization and phylogenetic analysis using internal transcribed spacer regions of rDNA. This species has not been previously reported in Korea.

Cladophialophora lanosa sp. nov., a New Species Isolated from Soil

  • Das, Kallol;Lee, Seung-Yeol;Jung, Hee-Young
    • Mycobiology
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    • v.47 no.2
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    • pp.173-179
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    • 2019
  • Cladophialophora is characterized by simple hyphomycetes with brown hyphae that give rise to branched chains of pale brown conidia and shows affinities with the Herpotrichiellaceae. A fungal strain belonging to the genus Cladophialophora was isolated from soil in Daegu, Korea. This strain produces numerous greenish to dark black lanose aerial mycelia with hair like structures. It is morphological similar to C. chaetospira, C. inabaensis, and C. multiseptata; however, the conidiophores and conidia sizes of the newly isolated strain (KNU16-032) are clearly different from them. The novelty of the strain was also confirmed based on phylogenetic analysis using the data sets of the internal transcribed spacer region of and the partial sequence of 28S ribosomal DNA region along with the cultural characteristics. Because morphological observations and phylogenetic analysis indicated that the strain is distinct from previously known Cladophialophora species, we propose this species as a new species Cladophialophora lanosa sp. nov., and provide the detailed descriptions in this study.

Morphology and Phylogeny of Two Novel Species within the Class Dothideomycetes Collected from Soil in Korea

  • Das, Kallol;Lee, Seung-Yeol;Jung, Hee-Young
    • Mycobiology
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    • v.49 no.1
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    • pp.15-23
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    • 2021
  • Two fungal strains (KNU-JJ-1827 and KNU-JJ-1829) belonging to the class Dothideomycetes were discovered from Jeju Island, Korea during this investigation of soil microfungi. Strain KNU-JJ-1827 showed fewer conidial septations, larger conidiogenous cells, and smaller conidia compared to the previously identified closest species of Didymocyrtis. Strain KNU-JJ-1829 revealed the similar characteristics of the nearest certain species of the genus Parathyridaria with the production of conidiogenous cells and conidia, because no asexual morphs were detected from the closest type strain Parathyridaria rosae. The novelty of the strains was also confirmed by analyzing molecular data using internal transcribed spacer regions and 28S rDNA. The molecular phylogeny also strongly support the detailed description and illustration for each proposed species as Didymocyrtis septata sp. nov. (KNU-JJ-1827) and Parathyridaria ellipsoidea sp. nov. (KNU-JJ-1829) isolated from soil in Korea.

Clonostachys divergens and Chrysosporium merdarium: Two New Records from Soil in Korea

  • Whee Phaund;Ung Somaly;Kallol Das;Seung-Yeol Lee;Hee-Young Jung
    • The Korean Journal of Mycology
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    • v.51 no.2
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    • pp.91-100
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    • 2023
  • During an investigation of micro-fungi in soil, two fungal isolates belonging to the phylum Ascomycota, namely KNUF-20-NI011 and KNUF-20-NI006, were collected from Gyeongbuk Province and Dokdo Island in Korea and identified as Clonostachys divergens and Chrysosporium merdarium, respectively. The fungal isolates were confirmed through molecular phylogenetic analyses of the internal transcribed spacer regions, 28S rDNA large subunit, and β-tubulin sequences. Cultural and morphological characteristics were observed and determined using different media. These species were identified based on phylogenetic relationships along with their cultural and morphological characteristics. To our knowledge, this is the first report on Clonostachys divergens and Chrysosporium merdarium in Korea.