• Title/Summary/Keyword: Sodium Bicarbonate

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A role of Sodium Bicarbonate Cotransporter(NBC) in $HCO_3^-$ Formation in Human Salivary Gland Acinar Cells

  • Jin, Mee-Hyun;Koo, Na-Youn;Jin, Mei-Hong;Hwang, Sung-Min;Park, Kyung-Pyo
    • International Journal of Oral Biology
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    • v.33 no.4
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    • pp.143-147
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    • 2008
  • The sodium bicarbonate cotransporter (NBC) protein is functionally expressed in salivary glands. In this experiment, we examined the role of NBC in $HCO_3^-$ formation in human parotid gland acinar cells. Intracellular pH (pHi) was measured in 2'-7'-bis(carboxyethyl)-5(6)-carboxyfluorescein (BCECF)-loaded cells. Acetazolamide (0.1 mM) and 4,4'-diisothio cyanatostilbene-2,2'-disulphonic acid (DIDS, 0.5 mM) were used as specific inhibitors of carbonic anhydrase and NBC, respectively. The degree of inhibition was assessed by measuring the pHi recovery rate (${\Delta}pHi$/min) after cell acidification using an ammonium prepulse technique. In control experiments, ${\Delta}pHi$/min was $1.40{\pm}0.06$. Treatment of cells with 0.5 mM DIDS or 0.1 mM acetazolamide significantly reduced ${\Delta}pHi$/min to $1.14{\pm}0.14$ and $0.74{\pm}0.15$, respectively. Simultaneous application of DIDS and acetazolamide further reduced ${\Delta}pHi$/min to $0.47{\pm}0.10$. Therefore, DIDS and acetazolamide reduced ${\Delta}pHi$/min by 19% and 47%, respectively, while simultaneous application of both DIDS and acetazolamide caused a reduction in ${\Delta}pHi$/min of 67%. These results suggest that in addition to carbonic anhydrase, NBC also partially contributes to $HCO_3^-$ formation in human parotid gland acinar cells.

Biochemical Characterization of 1-Aminocyclopropane-1-Carboxylate Oxidase in Mung Bean Hypocotyls

  • Jin, Eon-Seon;Lee, Jae-Hyeok;Kim, Woo-Taek
    • BMB Reports
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    • v.31 no.1
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    • pp.70-76
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    • 1998
  • The final step in ethylene biosynthesis is catalyzed by the enzyme 1-aminocyclopropane-1-carboxylate (ACC) oxidase. ACC oxidase was extracted from mung bean hypocotyls and its biochemical characteristics were determined. In vitro ACC oxidase activity required ascorbate and $Fe^{2+}$, and was enhanced by sodium bicarbonate. Maximum specific activity (approximately 20 nl ethylene $h^{-1}$ mg $protein^{-1}$) was obtained in an assay medium containing 100 mM MOPS (pH 7.5), $25\;{\mu}M$ $FeSO_4$, 6 mM sodium ascorbate, 1 mM ACC, 5 mM sodium bicarbonate and 10% glycerol. The apparent $K_m$ for ACC was $80{\pm}3\;{\mu}M$. Pretreating mung bean hypocotyls with ethylene increased in vitro ACC oxidase activity twofold. ACC oxidase activity was strongly inhibited by metal ions such as $Co^{2+}$, $Cu^{2+}$, $Zn^{2+}$, and $Mn^{2+}$, and by salicylic acid. Inactivation of ACC oxidase by salicylic acid could be overcome by increasing the $Fe^{2+}$ concentration of the assay medium. The possible mode of inhibition of ACC oxidase activity by salicylic acid is discussed.

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Effect of Bicarbonate and Phosphate Buffer Treatments on the Structure and Thermal Stability of Spent Layer Meat (중 탄산 및 인산염 완층액 처리가 노계육의 조직구조 및 열안정성에 미치는 영향)

  • Yi, Song-Sop;Mast, Morris G.
    • Korean Journal of Food Science and Technology
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    • v.23 no.6
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    • pp.695-701
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    • 1991
  • Spent layer breast meat and leg meat samples washed with 0.05 M sodium bicarbonate solution and 0.04 M phosphate buffer(pH 8.3) showed decreases in heat denaturation temperature indicating the destabilization of myofibrillar proteins. The destabilization was attributed to the solubilization of 95 Kdalton and 55 kdalton proteins from the myofibrils observed in gel-electrophoretograms. Transmission electron microscopy further indicated the breakage of Z-lines.

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Use of Chemical Treatments to Reduce Tannins and Trypsin Inhibitor Contents in Salseed (Shorea robusta) Meal

  • Mahmood, S.;Khan, Ajmal M.;Sarwar, M.;Nisa, M.;Lee, W.S.;Kim, S.B.;Hur, T.Y.;Lee, H.J.;Kim, H.S.
    • Asian-Australasian Journal of Animal Sciences
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    • v.20 no.9
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    • pp.1462-1467
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    • 2007
  • This study investigated the effect of chemical treatments on tannins (condensed and hydrolysable) and on the trypsin inhibitor (TI) activity in salseed meal. Triplicate samples of ground salseed meal (1 kg) were mixed with 820 ml of either distilled water (pH 5.3), 0.67 M acetic acid (pH 2.4), 0.67 M sodium bicarbonate (pH 8.2) or 2% polyvinyl-pyrrolidone (PVP) solution. The material was placed in airtight plastic containers and incubated at $37^{\circ}C$ for 0, 3, 6, 12, 24, 48 and 72 h. Samples of untreated salseed meal which had not been subjected to soaking or incubation were run through the analysis to serve as control. Addition of water, acetic acid, sodium bicarbonate and PVP solutions to salseed meal and subsequent anaerobic incubation at $37^{\circ}C$ significantly reduced chemically detectable tannins. At each incubation time, alkali solution was more effective than its counterparts. The effect of acidic solution on hydrolysable tannin was least among the treatments. All the treatments reduced TI activity of salseed meal. The reduction in TI activity by these treatments was similar and ranged between 80-84%. Treatment time effected a decrease in the contents of antinutritional substances. However, the effect of the treatment with the reagents, even for zero incubation time, was quite pronounced. It may be concluded from the present results that the treatment of salseed meal with sodium bicarbonate (0.67 M) is more effective in reducing hydrolysable and condensed tannin contents than PVP, water and acid solutions. Treatment with sodium bicarbonate solution is more economical and easier to handle than acid and PVP treatments. Incubation of the treated material for 12 h is reasonably effective, economical and safe from any mould growth.

Conjugated Linoleic Acid in Rumen Fluid and Milk Fat, and Methane Emission of Lactating Goats Fed a Soybean Oil-based Diet Supplemented with Sodium Bicarbonate and Monensin

  • Li, X.Z.;Yan, C.G.;Long, R.J.;Jin, G.L.;Shine Khuu, J.;Ji, B.J.;Choi, S.H.;Lee, H.G.;Song, Man K.
    • Asian-Australasian Journal of Animal Sciences
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    • v.22 no.11
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    • pp.1521-1530
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    • 2009
  • A metabolic study was conducted with four ruminally-cannulated lactating goats (Saanen, 29 weeks lactation, 65${\pm}$5 kg) in a 4${\times}$4 Latin square design with 4 dietary treatments. The goats were fed a basal mixed diet consisting of 80% concentrate and 20% chopped rye grass hay (DM basis, CON). The goats were also fed the CON diet supplemented with soybean oil at a 5% level of the concentrate (SO), the SO diet supplemented with 0.5% of sodium bicarbonate (SO-B) or the SO-B diet supplemented with 30 ppm monensin (SO-BM). The goats were housed in individual pen and the study was conducted for 8 weeks. An increased molar proportion of propionate (C3) was observed at 1 h (p<0.003) and 6 h (p<0.029) post-feeding from all the supplemented diets. Calculated methane emission was markedly decreased prior to morning feeding (p<0.01), and at 1 h (p<0.05) and 6 h post-feeding (p<0.05) in goats fed the supplemented diets. All the supplements increased (p<0.0001) cis9, trans11-CLA content in rumen fluid. Concentrations of both cis9, trans11-CLA (p<0.0001) and trans10, cis12-CLA (p<0.026) were also increased in the milk fat of lactating goats fed the supplemented diets. The SO-B and SO-BM diets further increased CLA content in goat milk compared to the SO diet. All supplements increased unsaturated (UFA, p<0.002), monounsaturated (MUFA, p<0.002) and polyunsaturated fatty acids (p<0.014) and reduced SFA to UFA ratio (p<0.023). The concentration of MUFA was even greater (p<0.002) for SO-BM than for the SO-B diet. In conclusion, feeding soybean oil (5% of concentrate) to lactating goats was a useful way to improve milk fat and to improve fatty acid profile in the milk by increasing potentially healthy fatty acids such as CLA. Supplementation of sodium bicarbonate or sodium bicarbonate with monensin to the soybean oil-based diet increased CLA content further in goat milk. Supplementation of soybean oil may be an effective method to reduce methane emission in lactating goats.

The effect of acid and alkali treatment on extracting nutrients from beef bone (소의 사골(四骨) 중(中)의 영양성분 용출에 대한 산, 알카리 처리효과)

  • Park, Dong Yean;Lee, Yeon Sook
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.12 no.2
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    • pp.146-149
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    • 1983
  • An experiment was made to investigate the effect of acid and alkali treatments obfserving the amount of nutrients especially calcium(Ca), phosphorus(P), ${\alpha}$-amino nitrogen(${\alpha}$-amino N), and total nitrogen(total-N)which were extracted from beef bone. 0~0.8% acetic acid and 0~0.5% sodium bicarbonate were used for treatment on different levels. The results are as follows; In case of acid treatment, Ca and P contents in bone stock wer observed to increase significantly according to acid concentration. Ca and P contents in bone stock highly incrased especially when the acid concentration was above 0.3%. Ca and P ratio varied from one to two when the acid concentration was below 0.01%. However contents of ${\alpha}$-amino N and total-N increased significantly when acid concentration was above 0.5%. In case of alkali treatment, contents of Ca and P did not increased significantly on all levels of sodium bicarbonate. The content of ${\alpha}$-amino N, however, increased significantly when the alkali concentration was in 0.05% but total-N did not show any variation in amount. These results suggest that acid treatment can give rise to an increase of the amount of nutrients which are extracted from beef bone but alkali treatment does not.

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Chemical Treatment for the Destruction of Aflatoxins in Laboratory Waste Water (실험실 폐수중 Aflatoxin 감소를 위한 화학적 처리에 관한 연구)

  • 김종규
    • Journal of Environmental Health Sciences
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    • v.18 no.2
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    • pp.52-56
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    • 1992
  • The ability of chemicals, 10% sodium hypochlorite, 28% ammonium hydroxide, 5% sodium hydroxide, 5% sodium bicarbonate, 0.1% hydrochloric acid, 5% hydrogen peroxide, and 5% acetone, to destroy aflatoxins in laboratory waste water containing 3.26 ppb of B$_{1}$ 7.64 ppb of B$_{6}$3 ppb of G$_{1}$, and 11.39 ppb of G$_{2}$ with the total of 29.11 ppb was investigated. High performance liquid chromatograph (HPLC) was used for the separation and quantitation of aflatoxins. Treatment for 2 hours by the chemicals affected the destruction of aflatoxins and the most effective chemical was 10% sodium hypochlorite (p<0.05). Sodium hypochlorite concentrations more than 1% significantly reduced aflatoxin B$_{2}$, G$_{1}$, G$_{2}$ and total aflatoxins and more than 3% reduced B$_{1}$ (p<0.05). No further significant decreases were observed above the concentration of 5% for all 4 aflatoxins. Complete destruction of aflatoxins B$_{2}$, G_{1}$, and G$_{2}$ was achieved by 5% sodium hypochlorite at 48 hours and B$_{1}$ at 72 hours.

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Studies on the Development of Sustained Release Preparation (I) Preparation and Evaluation of CAP Microcapsules of Sodium Ascorbate (지속성 제제의 개발에 관한 연구 (I) 아스코르빈산 나트륨의 CAP 마이크로캅셀의 제조 및 평가)

  • Shin, Sang-Chul;Koh, Ik-Bae
    • Journal of Pharmaceutical Investigation
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    • v.21 no.4
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    • pp.253-262
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    • 1991
  • Microencapsulation of sodium ascorbate with cellulose acetate phthalate(CAP) by coacervation/ phase separation method were carried out. Various factors affecting microencapsulation, i.e., surfactant concentration. CAP concentration, stirring speed and treatment of spermaceti as a sealing agent were studied. Dissolution rate. particle size distribution, surface feature and stability test were investigated. CAP microcapsules prepared using 0.5% span 80 as a surfactant showed smooth and round surfaces. The release of sodium ascorbate was retarded by microencapsulation with CAP and by sealant treatment with spermaceti. When triturated with sodium bicarbonate, CAP microcapsules were more stable than unencapsulated sodium ascorbate under various RH conditions at $37^{\circ}C$.

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Cholinergic Control of Pancreatic Secretion: The Effects of Atropine on Plasma Cholecystokinin and Secretin Release

  • Jo, Yang-Hyeok;Rhie, Duck-Joo;Chang, Young-Soon;Hahn, Sang-June;Sim, Sang-Soo;Kim, Myung-Suk;Kim, Chung-Chin
    • The Korean Journal of Physiology
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    • v.25 no.1
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    • pp.27-35
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    • 1991
  • Generally, it has been known that cholecystokinin (CCK) release into the plasma is under cholinergic control, but secretin release is not. Thus in anesthetized dogs we studied the effect of atropine $(50\;{\mu}g/kg\;followed\;by\;50\;{\mu}g/kg/hr)$ on pancreatic secretion and plasma concentrations of bioactive CCK and immunoreactive secretin in response to intraduodenal perfusion of sodium oleate (1, 3 and 9 mmol/hr). The volume, protein output and bicarbonate output of the secretion were increased by sodium cleats and this oleate-induced secretion was decreased significantly by atropine administration. However the increased plasma CCK and secretin levels by sodium oleate were not changed by atropine. These results indicate that atropine suppressed sodium oleate-induced pancreatic secretion through inhibiting cholinergic mechanism directly rather than decreasing the release of pancreatic secretory hormones. In another set of experiments, bilateral cervical vagi were stimulated electrically to observe the changes of pancreatic secretion and the above two plasma hormone levels in the presence or absence of atropine. In the vagally stimulated dogs, the volume, protein output and bicarbonate output of the pancreatic secretion were increased significantly. Both plasma secretin and CCK were concomitantly released significantly by vagal stimulation. Atropine significantly depressed the pancreatic secretory response as well as the release of these two pancreatic secretory hormones. Therefore, we conclude that in the presence of atropine the depressed pancreatic response to vagal stimulation is at least, in part, due to decreased release of endogenous CCK and secretin. In the vagally stimulated animals, however, the involvement of direct cholinergic influence on pancreatic exocrine gland remains to be answered.

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Studies on Transport Mechanisms of Turtle Bladder I . Epithelium of Urinary Bladder (Turtle bladder의 수송기작(輸送機作)에 관한 연구 : I. 방광(膀胱)의 상피조직(上皮組織))

  • Jeon, Jin-Seok
    • Applied Microscopy
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    • v.19 no.2
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    • pp.119-137
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    • 1989
  • It has been shown in this and earlier investigation that the turtle bladder mucosa has three main cell types on their mucosal surface. They are the granular cells, ${\alpha}$ CA cells, and ${\beta}$ CA cells. The three major transport mechanisms that occurs in the turtle bladder are sodium reabsorption, proton secretion, and bicarbonate secretion. In the present work the trans-port mechanisms by bladder epithelial cells of freshwater turtle, Pseudemys scripta, are summarized as follows. 1. The granular cells play an important role in sodium transport, while the ${\alpha}$ and ${\beta}$ CA cells do not appear to play a determining role in sodium transport. 2. It appears that the active sodium transport in the granular cells occurs in two-step process, implying that first, sodium diffuses into the cells, followed by an energy-dependent efflux step, which is catalyzed by the ouabain-sensitive Na-K ATPase. 3. The ${\alpha}$ type of CA cells are responsible for the proton secretion using the proton pump on the apical plasma membrane, while the ${\beta}$ type of CA cells are believed to be responsible for bicarbonate secretion. 4. When looked at under freeze-fracture electron microscopy, the apical plasma membrane of ${\alpha}$ cells have a characteristic population of rod-shaped intramembranous particles which are believed to be components of the proton pumps. Conversely, ${\beta}$ type of CA cells show rod-shaped particles in their basolateral plasma membranes, which is consistent with the proton absorptive, bicarbonate secretory mechanism. 5. In the turtle bladder, the ${\alpha}$ and ${\beta}$ type of cells are believed to be both responsible for proton transport, but in opposite directions.

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