• Restorative Dentistry and Endodontics
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• 제27권5호
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• pp.535-542
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• 2002

The purpose of this study was to evaluate the effect of EDTA irrigant according to application time and temperature. 31 human mature extracted teeth with a single canal were sectioned with microtome in 3mm thickness and gained 62 samples of root canals. They were distributed randomly into 6 groups of 10 specimens each and control group of 2 specimens. Each specimen was prepared with GT rotary file (Dentsply, Maillefer Co., Swiss) and irrigated with 3 ml sodium hypochlorite every minute. Then smear layer was removed with EDTA solution (PULPDENT$^{\circledR}$, PULPDENT Co., USA.) except two control specimens. Specimens of each group were irrigated with 17% EDTA. The time and temperature of application were as follows : (Table omitted) All specimens were split longitudinally and prepared for examination by scanning electron microscopy. A set of reference micrographs was used to award a debris score as follows: 0 = no smear layer, all tubules clean and open; 1 = no superficial smear layer, tubule openings visible, but some contain debris plug or soft tissue remnants: 2 = moderate smear layer, some tubules open and others closed; 3 = heavy smear layer, most/all tubule openings obscured. Results were evaluated with Kruskal-Wallis test to determine whether there was statistically significant difference among six groups. Pairs of groups were analyzed using the Student-Newman-Keuls Method and Mann-Whitney test. The results were as follows : 1. Control specimens showed heavy smear layer at the canal walls 2. Among the groups applied with EDTA for 2 minutes, group 1 showed the heaviest smear layer, and there was statistically significant difference between group 1 and the other groups(p<0.05). 3. Among the groups applied with EDTA for 5 minutes, group 4 and group 6 showed smear layer but there was no significant difference between them. 4. Among the groups applied with EDTA for the same temperature, group 1 showed heavier smear layer than group 4, and there was statistically significant difference(p<0.05). 5. Among the groups applied with EDTA for the same temperature, group 2 showed heavier smear layer than group 5 and group 3 showed heavier smear layer than group 6. But there was no statistically significant difference among them. From the results above, it could be concluded, EDTA solution is effective in removing of smear layer when it is applied for 5 minutes. If EDTA is applied for 2 minutes, it should be applied above room temperature.

• 대한치과보존학회:학술대회논문집
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• 대한치과보존학회 2002년도 추계학술대회
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• pp.720-720
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• 2002

A number of investigations have shown that the presence of bacteria is prerequisite for developing pulpal and/or periradicular pathosis. Depending on the stage of pulpal pathosis, various species of bacteria can be cultured from infected root canals. Kakehashi et al. showed that exposure of pulpal tissue in germ-free rats was characterized by minimal inflammation and dentinal bridging while exposure of pulpal tissue in conventional rats with normal oral flora was characterized by pulpal necrosis, chronic inflammation, and periapical lesions. Currently used methods of cleaning and shaping, especially rotary instrumentation techniques, produce a smear layer that covers root canal walls and the openings to the dentinal tubules. The smear layer contains inorganic and organic substances that include fragments of odontoblastic processes, microorganisms, their by products and necrotic materials. Because of its potential contamination and adverse effect on the outcome of root canal therapy, it seems reasonable to suggest removal of the smear layer for disinfection of the entire root canal system. Presence of this smear layer prevents penetration of intracanal medications into the irregularities of the root canal system and the dentinal tubules and also prevents complete adaptation of obturation materials to the prepared root canal surfaces. Removal of the smear layer by an intracanal irrigant and placement of an antibacterial agent in direct contact with the content of dentinal tubules should allow disinfection of this complex system and better outcome for the root canal therapy. A new solution, which was a mixture of a tetracycline, an acid, and a detergent(MTAD), was developed in the Department of Endodontics, Dental School. Lorna Linda University, USA. It has been demonstrated that MTAD was an effective solution for the removal of the smear layer and does not significantly change the structure of the dentinal tubules when used as a final irrigant in conjunction with 1 % NaOCl as a root canal irrigant. Studies are in progress to compare the anti- microbial properties of this newly developed solution with those of sodium hypochlorite and EDTA that are currently used to irrigate the root canals and remove the smear layer from the surfaces of instrumented root canals.canals.

• Journal of Periodontal and Implant Science
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• 제30권1호
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• pp.121-134
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• 2000

Root conditioning has introduced to dissolve the smear layer and to produce surface demineralization, resulting to exposure of dentin collagen fibril and opening of dentinal tubules. The purpose of the present study was to examine the effect of different concentration and application time of tetracycline-HCL on root conditioning. Total 40 root specimen were prepared from 20 periodontitis-prone human single rooted tooth. The specimen were treated with tetracycline-HCL solution(20mg/ml, 50mg/ml, 100mg/ml)for 20 sec, 3 min, 5 min., and saline for 30 sec. The application method was rubbing method with cotton pellet. Under the scanning electron microscopy(20KV), the extent of smear removal and opening of the dentinal tubules were examined at x 3000. The following results were obtained. 1. Treatment of root specimen with saline did not remove the smear layer and open the dentinal tubules. 2. Treatment of root specimen with different concentration of tetracycline-HCL for 20 sec also did not remove the smear layer completely. 3. Treatment of root specimen with different concentration of tetracycline-HCL for 3 min opened the dentinal tubules and removed smear layer. 4. Treatment of root specimen with 50mg/ml of tetracycline-HCL for 3 min showed collagen fibril within the opened dentinal tubules. In conclusion, the effect of root conditioning with tetracycline-HCL is more dependent on the application time than the application concentration. Root conditioning with 50mg/ml tetracycline-HCL for 3 min is enough for obtaining the periodontal regeneration.

• 대한전자공학회논문지SP
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• 제47권6호
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• pp.107-116
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• 2010

스미어 현상은 CCD 센서의 전하 전달 과정에 기인한다. 정지 영상에서는 기계적 셔터와 같은 하드웨어적인 구조로 이를 제거하지만, 동영상에서는 수행 시간의 제약 등의 문제로 이러한 방식이 적용될 수 없다. 본 논문에서는 신호처리 관점에서 스미어 현상을 제거하여 정지 영상뿐만 아니라 동영상에도 적용이 가능한 스미어 제거 방식을 제안한다. 제안하는 방식은 CCD 센서의 모서리 부분에 존재하는 화소들의 집단인 광학암흑영역(optical black region)을 이용한다. 광학암흑영역은 실제로 빛의 영향을 받지 않아 상이 맺히지 않지만, 스미어가 발생할 경우 전하 전달에 의하여 스미어 신호가 저장된다. 따라서 제안하는 방식은 스미어 신호를 정확하게 추정하기 위해서 광학암흑영역에 발생한 신호로부터 스미어와 잡음에 의한 영향을 구분하고 잡음은 제거하면서 스미어 신호는 유지하는 과정을 선행하며, 이렇게 처리된 광학암흑영역 신호를 영상 전반에 빼주어 스미어 현상을 제거한다. 또한 스미어 현상에 의해 포화가 발생한 경우는 손실된 원 신호의 정보를 주변의 화소 값으로 대체하여 스미어 현상에 의한 시각적 열화를 개선한다. 실험 결과를 통해 제안하는 방법이 기존 방법에 비해 시각적인 면에서 뛰어난 결과를 보임을 확인할 수 있다.

• 한국지반환경공학회 논문집
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• 제13권8호
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• pp.85-94
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• 2012

실내시험을 통하여 현장여건을 고려한 스미어 영향을 평가하기 위하여 3종류(C:M=1:0, C:M=0.5:0.5, C:M=0:1)의 재성형 시료를 이용하여 다양한 조건의 3차원 실내 모형시험을 30종 실시하여 스미어 존의 발생범위를 측정하고 대표적인 위치에서 시료를 채취하여 스미어 존 내에서의 투수계수의 변화특성을 검토하였다, 연구결과, C:M=1:0(점토 100%)의 경우 맨드렐 관입길이, 맨드렐의 크기, 토압고려여부, 단위중량 및 맨드렐의 형상의 순으로 스미어 존의 발생범위에 큰 영향을 미치는 것을 알았다. 따라서, 현장에서 맨드렐의 관입, 인발의 영향을 크게 받는 장척의 배수재를 설치하는 경우에는 기존의 실내시험을 통한 연구결과에 비해 스미어 존이 더욱 크게 형성되며, 스미어 존(smear zone)의 직경 ($d_s$)은 맨드렐(mandrel) 환산직경($d_m$)의 약 1.89~2.48배로 나타났다. 지반밀도와 맨드렐관입특성이 동일한 조건인 경우에 실트의 함유량이 높은 지반일수록 스미어 존의 발생범위는 넓어지는 것을 알았다. 현장의 토압이 크게 고려될수록, 맨드렐 사이즈가 클수록, 단위중량이 작을수록 스미어 존의 발생범위는 적게 나타났다. 맨드렐 관입, 인발에 의한 투수계수 비($k_{hs}/k_{ho}$)는 재성형시료를 사용한 본 실험에서 평균적으로 약 0.70~0.85의 범위를 나타냈으며, 투수계수저하의 영향요인과 영향치는 스미어 존의 발생범위와 유사하게 나타나는 것을 알았다.

• 한국방재학회:학술대회논문집
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• 한국방재학회 2007년도 정기총회 및 학술발표대회
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• pp.542-545
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• 2007

The vertical drain method generate the disturbed zone, because the drainage should be penetrated into the in-situ ground. The characteristics of the smear zone generated cause the problems that the coefficient of permeability decreases and the consolidation time anticipated in the design is not properly shortened. In this study, in order to understand such influence, the laboratory test were carried out so as to reasonably determine the coefficient of consolidation in silt. For that procedure, mandrel of a diversity were penetrated into the soil tube with silt, and then the variation of pore water pressure was measured with the pore pressure meter, Then, the range of smear effect on silt was estimated with monitoring data through the laboratory test, and also analyzed with inputting the coefficient of consolidation into the theoretical equation.

• Restorative Dentistry and Endodontics
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• 제16권1호
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• pp.167-180
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• 1991

The purpose of theis study was to evaluate the effectiveness of ultrasonic instrumentation in removing debris and smear layer from the root canal walls. 54 extracted, permanent single rooted teeth were randomly divided into 6 groups of 9 teeth. After canal preparation, the teeth were longitudinally sectioned. The sepcimens were then naturally dried for 2 days, given a maximun thickness gold coating, and examined under the SEM (JSM-35C type, JAPAN). Photographs of all specimens were then taken of the middle and the apical third of the root canal wall. The results were as follows: 1. In all groups, debris and the smear layer were not completely removed from the canal wall 2. There were no significant differences between at the apical third and at the middle third in removing debris and the smear layer in all groups. 3. There were no significant difference between the step - back group and the ultrasound group in removing debris and smear layer. 4. In general, the step - back/ultrasonund groups showed greater canal debridement than the step - back group or ultrasound group. 5. The step - back/ultrasound group with a No. 25 file for 3 min. showed significantly greater canal debridement than the step-back group (p<0.05), or the other step - back/ultrasound groups(p<0.05).

• Journal of Preventive Medicine and Public Health
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• 제37권2호
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• pp.166-173
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• 2004

Objectives : Cervical cancer is the second most frequent cancer among women in Busan. The Pap smear test could have a significant effect on detecting cervical cancer, and enhancing their rate of use is an important strategy for reducing the incidence and mortality of cervical cancer. This study aimed to evaluate the factors associated with the past use of the Pap smear test in Korean women. Methods : A population-based survey was carried out in Busan between November 1999 and March 2000. 1,673 participants were randomly selected from 2,684 women in Busan, using a 2-stage cluster sampling method, and interviewed in their homes. Their socio-demographic characteristics, smoking, drinking, familial cancer history, Pap smear screening history, reproductive and menstrual factors, sexual habits and use of contraceptive methods data were collected by a trained interviewer using a questionnaire. The use of the screening test was defined by a self-report from the participants on how many times they had had a Pap smear test in their lifetime, and when they had received their latest examination. Results : Of the 1,673 respondents (62.3% response rate), 57.6% had had a Pap smear test during her life (mean number, 2.3). Among the health examination participants(1,064), 961(90.3%) reported having sexual experience and 70.9% of these had had a Pap smear test. In a multiple logistic regression analysis, statistically significant relationships were observed for age groups and the Pap smear test rate (odds ratio, OR for 35-44 years=2.45; OR for 45-54 years=3.41; OR for 55 years=2.60; reference, under 34 years). The married or cohabiting women were more likely to have used the Pap smear test than those separated or widowed (OR=1.73). Among the reproductive behavioral measures, the number of births (OR for 3 births=4.22; OR for 2 births=3.95; OR for 1 births=3.38; reference, 0 births) and husband's extra-marital affairs (OR=1.50) were associated with the rates of use of Pap smear tests. Conclusion : It appears that the most important contributing factors to cervical cancer screening were age, marital status and number of births. A positive association was also observed for the husband's extra-marital affairs. This study enabled us to systematically assess the relationship between Pap smear rates and risk factors for cervical cancer. It is hoped that this study will make a significant contribution to the accumulating scientific evidence on the identification of factors associated with cervical cancer screening in Korea.

• Journal of Radiation Protection and Research
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• 제37권4호
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• pp.226-230
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• 2012

방사선 사고시 피폭환자로부터 채취된 스메어 시료의 전알파 방사능 측정법을 수립하였으며 이를 바탕으로 피폭환자의 후속조치 절차를 확립하였다. 국내방사선사고 대응기관에서 사용중인 스메어용 면봉을 이용하여 계측 절차를 검증하였다. 액체섬광계수기를 이용하여 표준선원을 점적한 시료의 계측 결과 20% 이내에서 인증값과 잘 일치하였으며, 채취 조건은 세정제 등을 이용하는 것이 더 높은 스메어 효율을 보였다. 액체섬광계수법 특성상 소광현상의 영향을 배제하기 위해 건조 등의 최소한의 전처리가 필요 할 것으로 판단되었다. 계측결과를 바탕으로 방사선비상시 피폭환자에 대한 의료적 처치 기준 및 선량평가 절차를 수립하였다.

• Restorative Dentistry and Endodontics
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• 제25권1호
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• pp.116-122
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• 2000

On the instrumented root canal wall, amorphous, irregular smear layer can be observed with Scanning Electron Microscope(SEM). The purpose of this study was to evaluate the effect of the presence or absence of smear layer on the adhesion of Staphylococcus aureus to the dentin of the root canal. Human incisors, extracted within 7 days, with no caries, no fracture, no calcification of canal, were selected. After cutting crown portion at cemento-enamel junction, root canal preparation was done by modified crown-down technique using Profile and Gates - Glidden Drill. During canal preparation, 10ml physiologic saline solution(group1&3) or 10ml 3.5% NaOCl(group2&4) was used as irrigation solution. And 10ml physiologic saline solution(group1&3) or 10ml 0.5M EDTA(group2&4) was applicated for final flush. After vertical sectioning and ethylene oxide gas sterilization, samples(group1&2) were immersed into BHIYHM broth inoculated with Staphylococcus aureus (ATCC 31153) and incubated for 3hrs at $37^{\circ}C$. All samples were prepared for and observed with SEM(JEOL JSM840S). The data were analyzed by Mann-Whitney rank sum test. The conclusions are as follows ; 1. Smear layer covers entire root canal surface after root canal preparation. 2. Smear layer has been removed away and the entrances of dentinal tubules have opened widely, when applying 0.5M EDTA and 3.5% NaOCl. 3. A significantly higher number of bacteria were adhered to the root canal dentin without smear layer(p<0.0001). 4. Smear layer produced during root canal preparation impedes bacterial adhesion and colonization to dentin matrix, therefore inhibits canal reinfection.