• BMB Reports
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• v.55 no.1
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• pp.48-56
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• 2022

Small extracellular vesicles (sEVs) secreted by most cells carry bioactive macromolecules including proteins, lipids, and nucleic acids for intercellular communication. Given that some immune cell-derived sEVs exhibit anti-cancer properties, these sEVs have received scientific attention for the development of novel anti-cancer immunotherapeutic agents. In this paper, we reviewed the latest advances concerning the biological roles of immune cell-derived sEVs for cancer therapy. sEVs derived from immune cells including dendritic cells (DCs), T cells, natural-killer (NK) cells, and macrophages are good candidates for sEV-based cancer therapy. Besides their role of cancer vaccines, DC-shed sEVs activated cytotoxic lymphocytes and killed tumor cells. sEVs isolated from NK cells and chimeric antigen receptor (CAR) T cells exhibited cytotoxicity against cancer cells. sEVs derived from CD8⁺ T and CD4⁺ T cells inhibited cancer-associated cells in tumor microenvironment (TME) and activated B cells, respectively. M1-macrophage-derived sEVs induced M2 to M1 repolarization and also created a pro-inflammatory environment. Hence, these sEVs, via mono or combination therapy, could be considered in the treatment of cancer patients in the future. In addition, sEVs derived from cytokine-stimulated immune cells or sEV engineering could improve their anti-tumor potency.

• Cartoon and Animation Studies
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• s.17
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• pp.147-161
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• 2009

Colors in design elements are visual non-verbal symbols both sensual and emotional. It is the first priority in the selection of products for the consumers, and also represents differentiated strategies and coherent integration symbols for the brand. Colors in advertising is an important tool to represent the long-term image management for the brand as well as the product image and concept of the advertise. The theme of this paper is to develop creative advertisement analyzing colors from automobile video ads. Analysis results showed that video ads with small cars under 2000cc preferred pastel colors with high saturation and brightness to emphasize on individuality rather than authority. On the other hand video ads with big and luxury cars upper 2000cc preferred dark colors with lower saturation and brightness. Small car video ads focused on product appearance showing the practical value of the car, big luxury car video ads focused on the symbolic image feeling the difference between the representation of the ads. Colors used in advertisement represents the sensitivity of the consumers. In video ads the concept of the color should be set by identifying on who the globule targets are and on what item you show by identifying the corresponding colors sensitivity to suit your advertising strategy will increase the value of the brand and products.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.17 no.11
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• pp.483-490
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• 2016

Automobile door latch is a fine design and assembly techniques are required in order to produce them in a small component assembly shape such as a spring, injection products, a small-sized motor. The door latch is fixed to not open the door of the car plays an important role it has a direct impact on the driver's safety. In this study, during trimming of the terminals of the connector main components of the car door latch, reduce rollover and conducted a research to find a suitable effective shear surface. Using the Taguchi method with orthogonal array of Finite Element Analysis and optimal Design of Experiments were set up parameters for the shear surface quality of the car door latch connector terminals. The design parameters used in the analysis is the clearance, the radius, and the blank holding force, the material of the connector terminal is a C2600. Trimming process optimum conditions suggested by the analysis has been verified by experiments, the shear surface shape and dimensions of a final product in good agreement with forming analysis results.Taguchi method from the above results in the optimization for the final rollover and effective shear surface improved for a vehicle door latch to the connector terminal can be seen that the applicable and useful for a variety of metal forming processes other than the trimming process is determined to be applicable.

• The Journal of Korean Society for Radiation Therapy
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• v.23 no.2
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• pp.109-117
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• 2011

Purpose: To analyze the accuracy of tumor volume dose following field width change, to check the difference of dose change by using self-made moving car, and to evaluate practical delivery tumor dose when tomotherapy in the treatment of organ influenced by breathing. Materials and Methods: By using self-made moving car, the difference of longitudinal movement (0.0 cm, 1.0 cm, 1.5 cm, 2.0 cm) was applied and compared calculated dose with measured dose according to change of field width (1.05 cm, 2.50 cm, 5.02 cm) and apprehended margin of error. Then done comparative analysis in degree of photosensitivity of DQA film measured by using Gafchromic EBT film. Dose profile and Gamma histogram were used to measure degree of photosensitivity of DQA film. Results: When field width were 1.05 cm, 2.50 cm, 5.02 cm, margin of error of dose delivery coefficient was -2.00%, -0.39%, -2.55%. In dose profile of Gafchromic EBT film's analysis, the movement of moving car had greater motion toward longitudinal direction and as field width was narrower, big error increased considerably at high dose part compared to calculated dose. The more field width was narrowed, gamma index had a large considerable influence of moving at gamma histogram. Conclusion: We could check the difference of longitudinal dose of moving organ. In order to small field width and minimize organ moving due to breathing, it is thought to be needed to develop breathing control unit and fixation tool.

• The Journal of the Korea institute of electronic communication sciences
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• v.10 no.3
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• pp.341-347
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• 2015

This study aims to design a chief VDR(Voyage Data Recorder) system which is appropriate to small and medium sized vessels and also implement the data about marine communication devices, sensors, etc. to be stored or printed at the navigator when those data are connected to VDR through data communication between marine navigation and VDR which are based on serial communication or internet in order to prove efficiency of the marine navigator. Also, the design of VDR is intended to be small and light in order to expand to apply it to small and medium vessels, which enables to analyze causes of marine accidents precisely through its characteristic functions which are the same as those at "vehicle mounted black-box" (location of the car, image and voice storage) by which the same roles are played on land.

• Journal of Veterinary Clinics
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• v.24 no.3
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• pp.422-425
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• 2007

A 6-month-old intact male Jindo dog was underwent surgery for hip fracture caused by hit by a car. Routine laboratory tests performed prior to surgery found no significant abnormalities, which might increase risks associated with general anesthesia. The dog was premedicated with atropine, induced general anesthesia with thiopental sodium and maintained with isoflurane. Forty minutes after surgery, the dog was suddenly bradycardic. Atropine (18 ug/kg) was slowly infused intravenously to normalize heart rate. However, paradoxically the dog showed slower heart rate with intermittent atrioventricular block ($2^{nd}$ degree type I) after atropine infusion. The dog's rhythm was returned to normal rate 7 minutes after ephedrine was infused. This is a rare case of paradoxical atrioventricular block induced by high dose of atropine in a dog.

• Journal of the Korean Society of Marine Environment & Safety
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• v.24 no.1
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• pp.126-132
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• 2018

This paper discusses about results of advanced buckling strength design for several kinds of perforated plated in the twin-hull car-ferry. For medium / small sized high speed vessels with a length of more than 50 meters and a length / width ratio of more than 12, such as car-ferries, it is highly possible that the buckling strength becomes weak due to the relatively thin thickness and the use of low strength capacity such as mild steel. Especially, it becomes big problem about weak buckling rigidity around the opening to access purpose in the perforated. As regarding safety design point of view for perforated plate, it is necessary to clarify buckling strength and ultimate strength by the distribution of in-plane load distribution around the opening. In this study, nonlinear series analysis using ANSYS was performed to clarify the influence of parameters such as aspect ratio, opening ratio and opening shape affecting the buckling and ultimate strength characteristics of the perforated plate under axial compression and we are derived the optimum design as buckling strength point of view. Based on these results, the governing factor determining the buckling strength of the perforated plate was the opening ratio, and the aspect ratio and the shape of the hole were not influenced.

• Molecules and Cells
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• v.38 no.3
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• pp.259-266
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• 2015

The regulation of flowering time has crucial implications for plant fitness. MicroRNA156 (miR156) represses the floral transition in Arabidopsis thaliana, but the mechanisms regulating its transcription remain unclear. Here, we show that two AGAMOUS-like proteins, AGL15 and AGL18, act as positive regulators of the expression of MIR156. Small RNA northern blot analysis revealed a significant decrease in the levels of mature miR156 in agl15 agl18 double mutants, but not in the single mutants, suggesting that AGL15 and AGL18 co-regulate miR156 expression. Histochemical analysis further indicated that the double mutants showed a reduction in MIR156 promoter strength. The double mutants also showed reduced abundance of pri-miR156a and pri-miR156c, two of the primary transcripts from MIR156 genes. Electrophoretic mobility shift assays demonstrated that AGL15 directly associated with the CArG motifs in the MIR156a/c promoters. AGL18 did not show binding affinity to the CArG motifs, but pull-down and yeast two-hybrid assays showed that AGL18 forms a heterodimer with AGL15. GFP reporter assays and bimolecular fluorescence complementation (BiFC) showed that AGL15 and AGL18 co-localize in the nucleus and confirmed their in vivo interaction. Overexpression of miR156 did not affect the levels of AGL15 and AGL18 transcripts. Taking these data together, we present a model for the transcriptional regulation of MIR156. In this model, AGL15 and AGL18 may form a complex along with other proteins, and bind to the CArG motifs of the promoters of MIR156 to activate the MIR156 expression.

• Journal of Korean Society for Atmospheric Environment
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• v.31 no.1
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• pp.54-62
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• 2015

Small scale paint overspray booths are being operated nationwidely, for repair of passenger car body parts. paint aerosols are emitted from the paint overspray booth in operations. In paint overspray booth operations without ventilation system and air pollutants collection unit, it may land on nearby equipment. In this study a removal of sticky paint aerosol for application of the small-scale overspray paint booth. it's cause the surface of filter bag from generated sticky paint aerosol. To remove adhesion of paint aerosol the agglomerating agents are injected and mixed with sticky paint aerosols prior to reach the filter bag. The paint spray rate was set as $10{\pm}5g/min$ from air-atomized spray guns in the spray booth, injection rate of agglomerating was $10{\pm}5g/min$ in the mixing chamber. The filtration velocity including air pollutants varied from 0.2 m/min to 0.4 m/min. Bag cleaning air pressure was set as $5.0kg_f/min$ for detaching dust cake from surface of filter bag. Bag cleaning interval at the filtration velocity of 0.2 m/min was around 3 times longer than that of the 0.4 m/min. The residual pressure drop maintained highest value at the highest filtration velocity. Fractional efficiency of 99.952%~99.971% was possible to maintain for the particle size of 2.5 microns. Total collection efficiency at the filtration velocity of 0.2 m/min was 99.42%. During this study we could confirm high collection efficiency and long cleaning intervals for the test with filtration velocity of 0.2 m/min indicating an optimal value for the given dimensions of the test unit and test operating conditions.

• Tunnel and Underground Space
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• v.27 no.1
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• pp.50-57
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• 2017

In this study, the experiment for determining shock loss at the branch is conducted for the design of network double-deck road tunnel ventilation. The shock loss coefficient that determines the quantity of shock loss has been considered only regarding the constant aspect ratio of circular or rectangular section. However the suggestion of shock loss coefficient is needed since the aspect ratio of double-deck road tunnel for small vehicle is considered around 1:3 with the low height in Korea. The experiment model was made with the scale of around 1:23 applying Reynolds similarity law, so that the shock loss coefficient on the branch of the large aspect ratio was measured. The result of the study showed that shock loss coefficients of both split branch and straight branch were measured two to three times higher than those calculated from the theoretical equation or design values of previous studies. Therefore the study resulted the effect of large aspect ratio on shock loss coefficient was huge, and it is expected that precise design value can be suggested for the design of network double-deck tunnel ventilation.