• Journal of Plant Biology
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• v.19 no.2
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• pp.49-61
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• 1976

A survey was conducted on the vegetation of Judo and Gamagseum in warm temperate zone of Southern Korea. They are covered with natural vegetation, evergreen forest dominated with Castanopsis cuspiadata in Judo and Machilus thunbergii in Gamagseum. Judo is a small island, ca. 1.75 ha, located within the Wando port. Since the flora of Wando including the Judo was reported in 1924, some investigator have reported evergreen trees of Judo. But the list of plant species of Judo is still unavailable. Gamagseum, located at the 15km morth of Judo, is a small island, ca. 1.45 ha, cosisting of two islands, Dae-o-do and So-o-do in low tide, and the report of its flora and any other survery on its is almost none. The vegetatons of Judo and Gamagseum are an example of natural forest vegetation occured rarely in warm temperate zone because of human disturbance in the southern coast zone of the Korean Peninsula. However, the ecological study of those vegetation has not ever been made, and the ecological or plant geographical situations of their vegetation is not clear. To determine the vegetation type, listing of plant species in the islands, calculation of basal area of trees over DBH=4.5cm, Raunkiaer's life form, leaf size class, Pte.-Q and etc., were studied. Total plant species of Judo was 110 species and that of Gamagseum was 99 species. In Judo, Castanopsis cuspidata was 1384 individuals among 2359 individuals over DBH=4.5cm, and in Gamagseum, Machilus thunbergii was remarkably abundant and Castanopsis cuspidata could not be found.

• Proceedings of the Plant Resources Society of Korea Conference
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• 2019.10a
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• pp.37-37
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• 2019

Cryopreservation is one of the ideal and suitable methods for long-term storage of plant germplasm. The plant contaminated with diseases and pathogens are decreased the multiplication rate, survival rate and high quality of plants after cryopreservation. The aim of this work was to improve a micropropagation method for lily in Korea, which are cultivars and indigenous plant. In the last process of rinsing scales after surface-sterilization, we tried to control the diseases and pathogens lived within the tissue by rinsing in 0.03% sodium hypochlorite (NaClO) instead of sterile distilled water. Bulb scales of Lilium were cultured in vitro on MS medium supplemented with Plant Preservative Mixture (PPM). The newly small bulb formed from bulb-scales was transferred to MS medium. We checked the non-contamination and survival rate after 2 weeks in culture. Non-contamination was shown to be 70 to 90% in formed small bulbs. This study will help to mitigate microbial contamination in Lilium species micropropagation for cryopreservation.

• Asian-Australasian Journal of Animal Sciences
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• v.29 no.2
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• pp.241-249
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• 2016

The supplementation of livestock feed with animal protein is a present cause for public concern, and plant protein shortages have become increasingly prominent in China. This conflict may be resolved by fully utilizing currently available sources of plant protein. We estimated the rumen degradability and the small intestinal digestibility of the amino acids (AA) in rapeseed meal (RSM), soybean meal (SBM), sunflower seed meal (SFM) and sesame meal (SSM) using the mobile nylon bag method to determine the absorbable AA content of these protein supplements as a guide towards dietary formulations for the dairy industry. Overall, this study aimed to utilize protein supplements effectively to guide dietary formulations to increase milk yield and save plant protein resources. To this end, we studied four cows with a permanent rumen fistula and duodenal T-shape fistula in a $4{\times}4$ Latin square experimental design. The results showed that the total small intestine absorbable amino acids and small intestine absorbable essential amino acids were higher in the SBM (26.34% and 13.11% dry matter [DM], respectively) than in the SFM (13.97% and 6.89% DM, respectively). The small intestine absorbable Lys contents of the SFM, SSM, RSM and SBM were 0.86%, 0.88%, 1.43%, and 2.12% (DM basis), respectively, and the absorbable Met contents of these meals were 0.28%, 1.03%, 0.52%, and 0.47% (DM basis), respectively. Among the examined food sources, the milk protein score of the SBM (0.181) was highest followed by those of the RSM (0.136), SSM (0.108) and SFM (0.106). The absorbable amino acid contents of the protein supplements accurately reflected protein availability, which is an important indicator of the balance of feed formulation. Therefore, a database detailing the absorbable AA should be established.

• Korean Journal of Plant Tissue Culture
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• v.27 no.1
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• pp.13-18
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• 2000

To investigate the function of chloroplast small HSP, transgenic tobacco plants (Nicotiana tabacum L. cv. Samsun) that constitutively overexpress the chloroplast small HSP (NtHSP21) from N. tabacum cv. Petit Havana SR1 were generated. Five homozygous lines of transformants showing different constitutive expression levels of the NtHSP21 were selected. To determine whether constitutive overexpression of NtHSP21 protein affects thermotolerance, wild-type and transformants were grown in Petri dishes, heat-stressed at 52$^{\circ}C$ for 45 min, and then incubated in normal growth condition. When heat-stressed wild-type plantlets were incubated at $25^{\circ}C$, leaf color gradually became white and all trio plantlets finally died within a week. As for the transformants, however, more than 70% of them remained green and survived under the conditions in which all the wild-type plants were dying. It was also found that the levels of NtHSP21 were correlated with the degree of thermotolerance. These results suggest that the NtHSP21 protein in transformants is responsible for the increase in thermotolerance.

• JOURNAL OF ELECTRICAL WORLD
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• no.7 s.91
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• pp.76-79
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• 1984

• Journal of Plant Biotechnology
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• v.43 no.3
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• pp.332-340
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• 2016

Seed size traits are controlled by multiple genes in crops and determine grain yield, quality and appearance. However, the molecular mechanisms controlling the size of plant seeds remain unclear. We performed functional analysis of BrPATL4 encoding Sec14-like protein to determine the genetic architecture of seed size, shape and their association analyses. We used 60 $T_3$ transgenic rice lines to evaluate seed length, seed width and seed height as seed size traits, and the ratios of these values as seed shape traits. Pleiotropic effects on general architecture included small seed size, erect panicles, decreased grain weight, reduced plant height and increased sterility, which are common to other mutants deficient in gibberellic acid (GA) biosynthesis. To test whether BrPATL4 overexpression is deleterious for GA signal transduction, we compared the relative expression of GA related gene and the growth rate of second leaf sheath supplied with exogenous $GA_3$. Overexpression of BrPATL4 did not affect GA biosynthesis or signaling pathway, with the same response shown under GA treatment compared to the wild type. However, the causal genes for the small seed phenotype (D1, SRS1, and SRS5) and the erection of panicles showed significantly decreased levels in mRNA accumulation compared to the wild type. These results suggest that the overexpression of BrPATL4 can control seed size through the suppression of those genes related to seed size regulation. Although the molecular function of BrPATL4 is not clear for small seed and erect panicles of BrPALT4 overexpression line, this study provides some clues about the genetic engineering of rice seed architecture.

• Journal of the Korean Society of Systems Engineering
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• v.14 no.1
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• pp.13-18
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• 2018

Plant Engineering is a series of activities going through following life cycle phases: planning, basic design, detailed design, procurement and construction, commissioning, operation and maintenance, to produce a target product. From among these phases of plant engineering life cycle, a detailed design phase is an important phase producing final design deliveries. Luckily, through technical co-operation and experiences of constructing plants, large Korean engineering companies have accumulated know-hows of efficient detailed designs. However, smaller engineering companies have less experience of performing detailed designs so there is always a risk of causing design errors in the detailed design phase. To mitigate the risk of design errors in the detailed design phase, it is necessary to systematize a concrete activity model of a detailed design phase. In this paper, we have developed a prototype of a detailed design activity model through a widely used function modeling methodology called IDEF0.

• Proceedings of the Korean Society of Plant Pathology Conference
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• 2003.10a
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• pp.139.1-139
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• 2003

Tissues from woody plant contain higher amount of phenolic compounds and polysaccharides, which give inhibitory effects on reverse transcriptase and/or Taq ploymerase. The common multiple-step protocols using several additives to inhibit polyphenoic compounds during nucleic acid extraction are time consuming and laborious. Sodium sulfite (Na$_2$SO$_3$) was used as inhibitor of polyphenolic oxidases in extraction buffer and compare it's effect between commercial RNA extraction kit and small-scale double-stranded RNA (dsRNA) extraction by RT-PCR. During nucleic acid extraction procedure, addition of 0.5％-1.5％ (w/v) sodium sulfite to Iysis buffer or STE buffer resulted in lighter color change than extracts without sodium sulfite and improve the RT-PCR detection. When commercial RNA extraction kit used, optimal concentration of sodium sulfite were variable according to the host plant. However, using dsRNA as RT-PCR template, 1.5％ sodium sulfite in STE buffer improves the detection of both viruses and unspecific amplifications were reduced significantly, Furthermore, when viruses existed at low titers in host plant, small-scale dsRNA extractions were very reliable.

• Nuclear Engineering and Technology
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• v.51 no.5
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• pp.1307-1313
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• 2019

Recent analysis results with realistic assumptions provide the variability of operator allowable time for the initiation of aggressive cooldown under small break loss of coolant accident or steam generator tube rupture with total failure of high pressure safety injection. We investigated how plant risk may vary depending on the variability of operators' failure probability of timely initiation of aggressive cooldown. Using a probabilistic safety assessment model of a nuclear power plant, we showed that plant risks had a linear relation with the failure probability of aggressive cooldown and could be reduced by up to 10% as aggressive cooldown is more reliably performed. For individual accident management, we found that core damage potential could be gradually reduced by up to 40.49% and 63.84% after a small break loss of coolant accident or a steam generator tube rupture, respectively. Based on the importance of timely initiation of aggressive cooldown by main control room operators within the success criteria, implications for improvement of emergency operating procedures are discussed. We recommend conducting further detailed analyses of aggressive cooldown, commensurate with its importance in reducing risks in nuclear power plants.

• Journal of the Korean Society of Systems Engineering
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• v.18 no.2
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• pp.24-39
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• 2022

For the success of the plant project, it is necessary to review the project's progress through technical and business reviews at an appropriate time, such as design and construction, and determine whether to invest or to proceed with the project to manage risks. In particular, since the plant development projects are not mass-produced, trial and error in design and construction can have severe impacts in terms of cost and schedule. To this end, gate reviews are currently being conducted in plants and other industrial sectors, but there are few studies on how to conduct gate reviews suitable for the plant field. In addition, there is little literature to refer to when conducting gate reviews. So, in this study, we present an overall framework that includes the types of gate reviews to be performed and items to be checked in each gate review on small and medium-sized plant development projects in which the owner directly develops and operates plants.