Genetic diversity and soybean sprout-related traits were evaluated in a total of 72 soybean accessions (60 Glycine max, 7 Glycine soja, and 5 Glycine gracilis). 100-seed weight (SW) was greatly varied and ranged from 3.2g to 32.3g in 72 soybean accessions. Positive correlation was observed between GR and hypocotyl length (HL), whereas negative correlation was observed between SW and hypocotyl diameter (HD). Re-evaluation by discarding two soybean genotypes characterized with low GR indicated that much higher correlation of sprout yield (SY) with HD and SW. Based on the principal component analysis (PCA) for sprout-related traits, 57 accessions were classified. Soybean genotypes with better traits for sprout, such as small size of seeds and high SY, were characterized with high PCA 1 and PCA 2 values. The seed size in second is small but showed low GR and SY, whereas the third has large seed, high GR and more than 400% SY. In genetic similarity analysis using 60 SSR marker genotyping, 72 accessions were classified into three major and several minor groups. Nine of twelve accessions that were identified as the representatives of soybean for sprout based on PCA were in a group by the SSR marker analysis, indicating the SSR marker selection of parental genotypes for soybean sprout improvement program.
Rhododendron keiskei var. hypoglaucum (Ericaceae) was recently reported in Korea, with a disjunct distribution on the southern islands of the Korean Peninsula. Although chromosome numbers and ploidy variations are important traits in angiosperms, gaining a clear understanding the cytological features of Rhododendron has been hampered by the small size of its chromosomes. We herein report the chromosome number, karyotype structure, and genome size of R. keiskei var. hypoglaucum for the first time. The chromosome number of the investigated plants was 2n = 26 with x = 13 as the base chromosome number, which is the one of the frequently encountered base chromosome numbers in Rhododendron. The karyotype of R. keiskei var. hypoglaucum is composed of metacentric and submetacentric chromosomes similar in length, which ranged from 1.39 to 2.40 ㎛. The DNA 1C-value in all examined accessions was small, ranging from 0.63 to 0.65 pg, further supporting the stable genome size in Rhododendron. These comprehensive cytological results provide a framework for detailed molecular, cytogenetic, and phylogenomic analyses that can be used to interpret the slow species diversification rate in Rhododendron.
Ryu, Sung Woo;Yoon, Kee Bong;Jeon, Gyu Min;Seong, Un Hak
Journal of the Korean Society for Nondestructive Testing
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v.36
no.6
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pp.504-509
/
2016
Defects in dissimilar metal welds are reported to be on the increase during the operating lifespan and aging of nuclear power plants. In Korea, reported cases of defects due to dissimilar metal welds include the drain nozzle of a steam generator and RCS hot tube sampling nozzles. Therefore, there is an urgent need to develop a reliable automated nondestructive inspection technique and a system for the inspection of dissimilar metal welds of small diameter nozzles in a high radiation area of a nuclear power plant. In this study, to ensure effective defect inspection of small diameter nozzles (RCS high-temperature tube sampling nozzle) of a nuclear power plant, three different methods were developed. These include: (1) optimum inspection probe design by beam simulation, (2) multi-directions UT optimum inspection technique for the inspection of small diameters of different welded parts, and (3) remote control automatic inspection system. The developed technique and systems have been verified to be suitable for use in the inspection of defects in smaller diameter nozzles in nuclear power plants.
Journal of the Korean Society of Environmental Restoration Technology
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v.7
no.5
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pp.26-37
/
2004
Most domestic small rivers and streams due to industrialization and urbanization have managed by concrete structures. The environmental functions of the river and stream are disappearing and urban streams play only the role of drainage systems. Also, the researches to restore natural streams are something yet to develop and not established the restoration for ecological functions of a small stream. Therefore the researches are required to develop ecological engineering system for watershed management system to handle various pollutants with restoration for ecological functions of a small stream. To develop this, the ecological engineering system for watershed management system could be developed with ecological conservation. In addition, ecological engineering system for watershed management system should be prior to conserve the habitat of biological resources and water conservation and applied to the original shape of streams. Also, it should be designed to restore the micro-topography of stream, the habitat of plant population in watershed. It is needed to develop the integrated researches to restore a small stream ecosystem.
In this study, we assessed dependency of small hydropower potentials on the two different runoff such as the estimated runoff based on the rainfall amounts and measured runoff. The hydpropower potentials were evaluated using actural power generations taken from Deoksong, Hanseok, and Socheon small hydropower plants over Han and Nakdong river basins, respectively. As a result of comparing the actual power generation amount with the potential amount based on the rainfall amount and the estimated amount based on the observed flow amount by each small hydroelectric power plant, the degree of latent small hydro energy by the observed flow was confirmed to be high. It is confirmed that the potential hydroelectric power generation rate is estimated to be about average 30%Point higher than the actual generation amount as a result of the measured flow rate rather than using the rainfall amount. Based on this, a method for improving the degree of the actual generation amount is proposed.
To obtain the fundamental informations on the varietal improvement of peanut and to study the ecological variations of the important agronomic traits and to the relationship between the traits studied, an investigation was made on varietal classification of 489 introduced on the basis of their morphological and ecological differences at Crop Experiment Station, Suweon in 1968, and the other study conducted at some location as above in 1969 was to investigate the ecological variations of the materials in accordance with changes of seeding date using classified varietal group under 5 different seeding times from April 16 to July 7 with twenty days interval. The results obtained were summarized as follows: 1. Peanut varieties tested were classified into Spanish, Virginia Erect, Virginia Runner, Southeast Runner. Valencia and Semirunner, on the basis of plant type, flowering time, number of grains per pod and grain size. 2. Characteristics of varietal group classified are as followings. (1) Spanish; erect, small grained and early maturing type. (2) Virginia Erect; erect, large grained and late flowering type. (3) Virginia Runner; runner, large grained and late maturing type. (4) Southeast Runner; runner, small grained and early maturing type. (5) Valencia; erect, small grained and early flowering type with 3-4 grains per pod. (6) Semi runner; semirunner, large grained and late flowering type. 3. Flowering period in respective varietal group was consistently shorted by delayed seeding date and the degree of shortening was more serious in late flowering varietal group. 4. Number of branches per plant was generally decreased in late seeding date in respective group. However, Spanish and Virginia Runner exhibited lower number of branches in the first seeding rather than the second seeding and the lowest number of branches was found in Spanish while the highest were Virginia Erect in all seeding date. 5. Shelling ratio was high in Spanish and Southeast Runner in any seeding date and decreased remarkedly by seeding after May. 6. Number of pod per plant in all varietal groups was remarkedly decreased by delayed seeding date and the degree of decreasing was more serious in large grain varietal group. 7. The higher pod weight per plant was found in second seeding date rather than first seeding and pod weight per plant was decreased obviously in all late seeding after the second. Therefore, among the cultivars tested, Southeast Runner noted the highest pod weight per plant while Virginia Runner showed the lowest. 8. Grain number per plant expressed the similar tendency as the pod weight per plant but was low in large grain group and high in small grain group in all seeding date employed. 9. 100 grain weight was heaviest in second seeding and was decreased remarkedly after the second and even the first seeding date. 10. Yield per 10a noted considerable variations in accordance with seeding date in all groups classified. However, the yield was increased in second seeding date (May 7) and decreased in the others. 11. Length of main stem and branches were exceptionally decreased in the first seeding date compare to the second in Spanish while other varieties were tend to be same between the indicated seeding date, but. these two traits were strikingly decreased in all seeding after the second. This tendency, however, strongly suggested the importance of environmental effects on peanut growth in terms of their changes due to the different seeding date. 12. Highly significant positive correlations were showed between yield and yield componets such as pod weight per plant, 100 grain weight and the number of grains per plant in all varietal groups except, Virginia Runner. However, the other characters were almost not correlated with yield and differences in correlation coefficients among the seeding dates were found. 13. Path coefficients estimated for yield components to yield was higher in number of grains per plant pod weight per plant and 100 grain weight in terms of direct effect and the other components were negligible in all varietal groups. 14. Heritabilities estimated were generally high in pod number per plant, shelling ratio, 100 grain weights and number of grains per pod and the other traits were relatively low.
Ju, Jiwon;Kim, Kangmin;Lee, Kui-Jae;Lee, Wang Hu;Ju, Ho-Jong
The Plant Pathology Journal
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v.33
no.1
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pp.53-65
/
2017
Barley yellow dwarf virus (BYDV) belongs to Luteovirus and is limited only at phloem related tissues. An open reading frame (ORF) 4 of BYDV codes for the movement protein (MP) of BYDV gating plasmodesmata (PD) to facilitate virus movement. Like other Luteoviruses, ORF 4 of BYDV is embedded in the ORF3 but expressed from the different reading frame in leaky scanning manner. Although MP is a very important protein for systemic infection of BYDV, there was a little information. In this study, MP was characterized in terms of subcellular localization and programmed cell death (PCD). Gene of MP or its mutant (ΔMP) was expressed by Agroinfiltration method. MP was clearly localized at the nucleus and the PD, but ΔMP which was deleted distal N-terminus of MP showed no localization to PD exhibited the different target with original MP. In addition to PD localization, MP appeared associated with small granules in cytoplasm whereas ΔMP did not. MP associated with PD and small granules induced PCD, but ΔMP showed no association with PD and small granules did not exhibit PCD. Based on this study, the distal N-terminal region within MP is seemingly responsible for the localization of PD and the induction small granules and PCD induction. These results suggest that subcellular localization of BYDV MP may modulate the PCD in Nicotiana benthamiana.
Malva vein clearing virus (MVCV) is a member of the Potyvirus species, and has a negative impact on the aesthetic development of Alcea rosea. It was first reported in Germany in 1957, but its complete genome sequence data are still scarce. In the present work, A. rosea leaves with vein-clearing and mosaic symptoms were sampled and analyzed with small RNA deep sequencing. By denovo assembly the raw sequences of virus-derived small interfering RNAs (vsiRs) and whole genome amplification of malva vein cleaning virus SX strain (MVCV-SX) by specific primers targeting identified contig gaps, the full-length genome sequences (9,645 nucleotides) of MVCV-SX were characterized, constituting of an open reading frame that is long enough to encode 3,096 amino acids. Phylogenetic analysis showed that MVCV-SX was clustered with euphorbia ringspot virus and yam mosaic virus. Further analyses of the vsiR profiles revealed that the most abundant MVCV-vsiRs were between 21 and 22 nucleotides in length and a strong bias was found for "A" and "U" at the 5′-terminal residue. The results of polarity assessment indicated that the amount of sense strand was almost equal to that of the antisense strand in MVCV-vsiRs, and the main hot-spot region in MVCV-SX genome was found at cylindrical inclusion. In conclusion, our findings could provide new insights into the RNA silencing-mediated host defence mechanism in A. rosea infected with MVCV-SX, and offer a basis for the prevention and treatment of this virus disease.
Dasgupta-Schubert, N.;Tiwari, D.K.;Francis, E. Reyes;Martinez Torres, P.;Villasenor Cendejas, L.M.;Lara Romero, J.;Villasenor Mora, C.
Advances in nano research
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v.5
no.3
/
pp.245-251
/
2017
Multiwalled carbon-nanotubes (MWCNT) and micro-structured carbon, such as biochar or activated carbon (AC), have been seen to significantly increase the growth indices of certain plant species such as maize (Zea mays L.). Seed imbibition is the stage where environmental factors that affect water transport across the seed coat barrier, make a large impact. This work explores the effect on water imbibition by maize seeds when the aqueous environment surrounding the seed is diluted by small concentrations (10 and 20 mg/l) of pristine MWCNT (p-MWCNT), carboxylate functionalized MWCNT (COO-MWCNT) and AC. The degree of sensitivity of the process to (i) large structural changes is seen by utilizing the nano (the MWCNT) and the micro (the AC) allotropic forms of carbon; (ii) to small changes in the purity and morphology of the p-MWCNT by utilizing 95% pure and 99% pure p-MWCNTs of slightly differing morphologies; and (iii) to MWCNT functionalization by using highly pure (97%) COO-MWCNT. Water imbibition was monitored over a 15 hour period by Near Infrared Thermography (NIRT) and also by seed weighing. Seed surface topography was seen by SEM imaging. Analysis of the NIRT images suggests rapid seed surface topological changes with the quantity of water imbibed. While further work is necessary to arrive at a conclusive answer, this work shows that the imbibition phase of the maize seed is sensitive to the presence of MWCNT even to small differences in the purity of the p-MWCNT and to small differences in the physicochemical properties of the medium caused by the hydrophilic COO-MWCNT.
Yoon, Min Soo;Song, Ki O;Lee, Jae Min;Ha, Seung Woo;Cho, Sun Young
Journal of the Korean Society of Safety
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v.33
no.5
/
pp.9-14
/
2018
The piping system of a nuclear power plant plays a role of transferring high energy fluid to equipment and various devices. The safety and soundness of these piping systems are very closely related to the operability of the power plant. In the case of a welded part of a small diameter pipe, it may grow as a microcrack due to a lack of penetration, and it may grow to a size that affects the safety of the pipe due to the influence of mechanical vibration and fatigue load. Resonance refers to an increase in energy as the natural frequency of an object coincides with the frequency applied to the external force. When this resonance occurs, the frequency is the resonance frequency. In this study, when defects exist in the welds of small diameter pipe, the natural frequency of the pipe changes and resonance may occur. Since these resonances are likely to cause fatigue damage to the piping, resonance frequency changes due to the size and shape of the defects are analyzed and evaluated. As a result of the vibration test, the resonance frequency tended to decrease as the depth of the defect deepened, and the influence was larger when the defect existed at the bottom of the top of the trough. Also, it was confirmed that the Transverse cracks had an effect on the resonance frequency in the presence of the cracks in the weld bead, compared to the longitudinal cracks. As a result of this study, it is expected that the cause of the defect and the condition of the pipe can be monitored because the resonance frequency tendency according to the shape of the crack is analyzed.
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