• Microbiology and Biotechnology Letters
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• v.40 no.1
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• pp.50-56
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• 2012

The Wnt/${\beta}$-catenin signaling pathway regulates diverse developmental processes and adult tissue homeostasis. Inappropriate regulation of this pathway has been associated with human diseases, such as cancers, osteoporosis, and Alzheimer's disease. Using a cell-based chemical screening with natural compounds, we discovered silybin, a plant flavonoid isolated from the Silybum marianum, which activated the Wnt/${\beta}$-catenin signaling pathway in a synergy with Wnt3a-conditioned medium (Wnt3a-CM). In the presence of Wnt3a-CM, silybin up-regulated ${\beta}$-catenin response transcription (CRT) in HEK293-FL reporter cells and 3T3-L1 preadipocytes through stabilization of intracellular ${\beta}$-catenin protein. Silybin and Wnt3a-CM synergistically reduced expression of important adipocyte marker genes including peroxisome-proliferator-activated $receptor{\gamma}$ ($PPAR{\gamma}$) and CAATT enhancer-binding protein ${\alpha}$ (C/$EBP{\alpha}$) in 3T3-L1 preadipocytes, accompanied by the activation of Wnt/${\beta}$-catenin signaling pathway. Taken together, our findings indicate that silybin is a small-molecule synergist of the Wnt/${\beta}$-catenin signaling pathway and can be used as a controllable reagent for investigating biological processes that involve the Wnt/${\beta}$-catenin signaling pathway.

• Journal of Korean Society of Environmental Engineers
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• v.38 no.6
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• pp.329-333
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• 2016

Disinfection by electrolysis would be useful for small wastewater treatment plant, combined sewer overflow, ballast water, swimming pool, and fish farming, where the transport, storage, and the use of chlorine gas is limited. This study investigated the feasibility of the electrolysis of dilute hydrochloric acid (HCl) for disinfection. The effects of HCl concentration, voltage and reaction time on the generation of oxidizing agents, HOCl, $O_3$, and $H_2O_2$, were examined in a series of batch test. The highest current efficiency was 99.3% which was found at 2.2%, 3 V, and 5 min of HCl concentration, voltage, and reaction time, respectively. Continuous electrolysis at 2.2% HCl, 3 V, and 5 min of the hydraulic retention time showed 97.4% of the current efficiency. Addition of sodium chloride up to 20 g/L linearly increased the oxidizing agents production. 92.2% of total coliforms were removed by the contact with the electrolyzed water.

• Journal of Korean Society of Forest Science
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• v.101 no.1
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• pp.130-137
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• 2012

This study has conducted to develop new gabion wall systems with vegetation base materials for stream bank stability and rapid rehabilitation. Vegetation base materials are primarily compounded with fine soil, organic composts and peat moss as plant fibers, a water retainer and a soil improver. Normally gabion wall systems resist the lateral earth pressures or stream power by their own weight. Therefore, fill material must have suitable weight, compressive strength and durability to resist the loading, as well as the effects of water and weathering. In this project, 100 to 200-mm clean, hard stones are basically specified, and about 50-mm rubbles are also used. Test application of new gabion wall system carried out in the stream bank of a small stream in the Gwangreung experimental forest, belonging to Korea Forest Research Institute (KFRI) in December 16th, 2006. As a result of the analysis of hydraulic stability of new gabion wall system, gabion wall system has highest threshold shear stress when the gabion wall covered by vegetation. New gabion wall system is highly resistant to sliding and overturning because safety coefficients exceed 1.5. As a result of term of slope stability analysis of new gabion wall system by Bishop and Fellenius methods, stability of stream bank was highly increased after the construction of gabion wall. Therefore, new gabion wall system is effective to stabilize unstable stream bank.

• Weed & Turfgrass Science
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• v.5 no.3
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• pp.155-164
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• 2016

Earworm is very useful animal in soil ecosystem, however it is harmful for golf courses because they introduce many cast on turfgrass that reduces turf uniformity and play quality. However, no information has found on earthworm diversity and seasonal fluctuation in different Korean golf courses. In this study, we focused to carry out earthworm species composition and seasonal population structure in turfgrass of golf courses. During spring and fall season survey with direct digging and tea saponin drenching sampling in 5 different golf courses, 6 species under 3 families of earthworms were collected. Earthworm species composition and density was different among the golf courses. Aporrectodea caliginosa in Lumbricidae was dominant species in Anseong and Dongrae Benest Golf Club; however Amynthas carnosus in Megascolecidae was dominant species in Anyang and Glenrose Golf Club. Ap. caliginosa was collected only aclitellum in July and Am. carnosus was collected aclitellum and clitellum in August in golf courses. Seasonal population of earthworm was different depending on earthworm species (Am. hupeiensis was the highest in August and Ap. caliginosa was in April) however small number of collected earthworm species were not dominant trend in golf courses.

• Weed & Turfgrass Science
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• v.5 no.4
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• pp.256-259
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• 2016

An obligate plant pathogenic fungus Puccinia zoysiae Dietel, the causal agent of rust disease, which is implicated in the damage of zoysiagrass (Zoysia spp.) in Korea. The fungus is one of the important pathogens, which attacks and colonizes susceptible zoysiagrass. Zoysia rust disease was observed in Jangseong, Hoengseong and Hapcheon in 2014 and 2015, Korea. The typical symptoms of the disease appeared first as small white spots on the leaf and stem of zoysiagrass plants, and turn brownish, dark brown spores revealed on the lesions. Uredospores were light brown in color, ellipsoid or spherical in shape and $22.0{\sim}25.0{\times}15.0{\sim}17.5{\mu}m$ in size. On the basis of the morphological characteristics, the fungus was identified as P. zoysiae. Pathogenicity of the fungus was proved by artificial inoculation on Z. japonica. The rust disease firstly appeared approx. early or middle June and then increased middle of October in Korea. In field, Z. matrella ecotypes were more resistant than Z. japonica ecotypes against the pathogen. Disease response against the pathogen was different among ecotypes of each species, respectively.

• Korean Journal of Organic Agriculture
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• v.10 no.2
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• pp.111-120
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• 2002

This study was conducted to investigate the effect of foliar application of Chitosan and seedling types on increasing the production of fall-growing potatoes(Solanum tuberosum) at the height of 250m from the sea in Jeju Island. The experiment main-plots consisted of four Chitosan applications, and subplots had three seedling types as cutting plug seedlings, mine-tuber plug seedling planting plot and seed potatoes planting plot. The foliar application of Chitosan of 2000ppm was carried out 0, 1, 3, 5 times at intervals of ten days after ten days of transplanting. Plant height and top dry weight were significantly more vigorous as three to five application of Chitosan. In the small tubers(under 80g), the number of tubers and tuber yield were relatively increased in the seed potatoes planting plot and mine-tuber plug seedling plot, but the large tubers(over 80g) was higher in the cutting plug seedling planting plot. T-N content in leaves was increased as the number of application of Chitosan was increased. A similar tendency was shown in K, P Ca, Mg and Na. The growth and tuber yield in cutting plug seedlings, mine-tuber plug seedling planting plot and seed potatoes planting plot were effectively increased as three to five foliar applications of Chitosan was increased.

• Journal of Food Hygiene and Safety
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• v.17 no.2
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• pp.101-105
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• 2002

A total of 53 vegetables and fruits, consisting of 23 leaf vegetables, 4 fruits vegetables, 4 edible roots,7 spice vegetables and 15 fruits were analyzed for contents of nitrate and nitrite by ion chromatography. Nitrite was detected 10 of 23 kinds of leaf vegetables, which was dominant in lettuce by average 349.9 mg/kg. Nitrate contents of leaf vegetables were average 578.3 mg/kg, 415.7 mg/kg, 348.6 mg/kg in wild plant, mustard leaf and chinese vegetable, respectively. Nitrite was not detected in fruits vegetables and rootcrops but the nitrate contents were abundant and the highest in squash(average, 86.2 mg/kg) and radish(average, 297.5 mg/kg), respectively. In spice vegetables, mate contents were from 0 to 29.8 mg/kg, but not detected in garlic. In welsh onion, its nitrite were higher in small type than large type, while nitrate contents were higher 3 times in the latter. Nitrate contents of fruits were lower in apple(average, 0.5 mg/kg) and higher in plum(average, 76.6 mg/kg) than other samples.

• Journal of the Korean Society of Food Culture
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• v.11 no.5
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• pp.699-710
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• 1996

The sacrificial foods using at 88 villages in Andong area where the village sacrificial rituals are performed, were investigated in 1994. The finding are as follows; 1. Since the village sacrificial rituals keep ancientry, white Baeksulki without any ingredients is used as Ddock (a steamed rice cake) in 74 villages, and in 5 villages the Baeksulki was served just in Siru (a tool used to prepare Ddock). The type of meat serving in rituals has been changing from large animal to small animal and the type of light from bowl-light to candle. 2. Though the ritual observes Confucianism style, memorial address was offered in 34 villages and only in 8 villages, liquor was served 3 times and the address was offered. In 60 villages, liquor was served only once, and calling village god, bowing twice, and burning memorial address paper were performed in order. Burning paper has been meaningful in rituals. 3. Tang (a stew) was served in 21 villages, and in 18 villages even Gook (a soup) was not used. The numbers of village which do not use cooked rice and Ddock are 14, respectively. 4. Fruits are essential in rituals foods. In 63 villages, 3 kinds of fruits are served and this is contrasted with even number of fruits in Bul-Chun-Wi Rituals. 5. In 25 villages, fish and meat are not served. As meat, chicken in 19 village are served. Meat Sikhae (a fermented rice beverage) prepared with meats or fish are served in 5 villages. Slice of dried meat is served in 73 villages commonly. 6. In gender of god for village shrine, woman in 43 villages, couple in 17 villages, man in 9 villages, mountain god in 2 villages, villages tree, etc were enshrined. In 52 villages liquor is served in rituals (liquor is used in 58 villages including serving god), in 28 villages sweet liquor (Gamju) is served for god (sweet liquor is used in 46 villages), and liquor, water, or sweet liquor is just displayed on table but not served in 8 villages. Incensing is found to be not common in village ritual, only in two villages it is performed. The most appropriate rituals food is thought to be pig for mountain god. Home dishes can not be used in village ritual and spoon is not used and chopstick is prepared from wild plant. Meat and fish are used in the raw.

• Journal of Bio-Environment Control
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• v.10 no.1
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• pp.36-41
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• 2001

This experiment was carried out to investigate temperature distribution in the double layer plastic greenhouse and chilling injury to watermelons grown during a cold season. Temperatures on eastern and western sides were about 6.2% and 14.7%, respectively, lower than that of central section in a south-north oriented greenhouse. Daily mean temperature in the northern part was about 1-2$^{\circ}C$ higher than that in the southern part of the greenhouse. In terms of vertical temperature distribution inside the greenhouse, temperature at ground surface was approximately 1$^{\circ}C$ lower during the day and 0.5$^{\circ}C$ higher during the night than that in the upper part, 2m from the ground surface. Leaf mould medium kept higher ground temperatures as compared to sandy soil, red clay soil, and in the northern and southern sides as compared to the central part of the greenhouse. A symptom of chilling injury on leaves was upward curling, followed by chlorosis and necrosis. A severe symptom of chilling injury to plants was the breakdown of vascular bundles. Root growth was more susceptible than stem or leaf growth to low temperatures. At 3$0^{\circ}C$, main and lateral roots grew vigorously, while lateral root growth was inhibited at 22$^{\circ}C$ and root growth was stopped at 14$^{\circ}C$ and 6$^{\circ}C$. Small and puffy fruits with dark green surface were produced at low temperatures. In cold season cultivation of watermelons, it is suggested that plants be transplanted in the central part and train to sides of the greenhouse in order to reduced chilling injuries.

• Journal of Environmental Impact Assessment
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• v.30 no.6
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• pp.361-379
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• 2021

About 10% of trees damaged by the development projects are to be transplanted when conducting the Environmental Impact Assessment. However, various problems have been raised during transplantation. In this study, we confirm the stakeholder's perceptions of the problems that occur during transplantation. The survey was conducted from October 9 to 25, 2020. Among the stakeholder groups, 36 respondents participated in the consulting institute group, 44 from the review institute group, and 83 from the developer·agency group (total of 163). All three groups responded that it was necessary to transplant some of the damaged trees even if the development charge increased because the damage caused by the development project was serious. The most serious problem was 'high mortality'. The response rate was high that all three groups should plant the same species with the same quantity as an alternative method in case of withering. In order to reduce the mortality rate, small-sized trees were transplanted and transplanted trees were expanded to include planted species and landscape trees. In addition, the number of transplanted trees was high in response to calculating the transplantratio to the number of native tree damaged. The percentage of respondents who said that it was necessary to allocate a separate manager was also high. The results will be used as basic data to improve problems that occur during transplantation of damaged trees.