• 식물병연구
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• 제15권2호
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• pp.57-62
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• 2009

우리나라 벼에 발생하는 주요 바이러스 중 애멸구에 의하여 전염하는 벼줄무의잎마름병(RSV)과 벼검은줄오갈병(RBSDV)에 대한 간편한 유전자 진단법인 VCHT-PCR 방법을 개발하였다. 벼 잎의 경우 즙액 추출 완충액은 0.5% sodium sulfite를 첨가한 0.01 M 인산완충액(pH 7.0)을 기본 완충액으로 이용하고 애멸구를 진단할 경우에는 기본 완충액에 2% PVP을 첨가하였을 때 VC/RT-PCR 진단이 잘 되었다. VC/RT-PCR을 이용한 진단에 적합한 RSV와 RBSDV 프라이머를 선발하였고 이것을 이용하여 동시진단으로 경기도 김포, 평택, 시흥지역에서 채집한 애멸구의 RSV와 RBSDV의 보독충을 쉽고 경제적으로 진단 할 수 있었다. ELISA에 의한 진단결과와 비교할 때 세 지역을 합하여 RSV에 대한 평균 보독충률은 9.2%로 동일하였으나 보독충 중 일부가 ELISA와 VC/RT-PCR 두 방법에 의한 진단결과가 다르게 나온 것은 RSV의 혈청학적, 유전적 계통 존재 가능성을 제시하고 있다. 포장에서 채집한 애멸구의 VC/RT-PCR 진단효율은 RSV와 RBSDV를 동시에 진단하므로 써 RSV만 진단이 가능한 ELISA 결과 보다 3.2% 높았다.

• 대한전기학회:학술대회논문집
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• 대한전기학회 1986년도 하계학술대회논문집
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• pp.173-176
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• 1986

• 한국원자력학회:학술대회논문집
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• 한국원자력학회 2004년도 추계학술발표회 발표논문집
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• pp.545-546
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• 2004

• 한국수자원학회:학술대회논문집
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• 한국수자원학회 2000년도 학술발표회 논문집
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• pp.779-783
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• 2000

• Journal of Electrical Engineering and Technology
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• 제9권5호
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• pp.1511-1519
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• 2014

The objective of this paper is to propose a modelling of a small compressed air energy storage system, which drives an induction generator based on a field-oriented control (FOC) principle for a renewable power generation. The proposed system is a hybrid technology of energy storage and electrification, which is developed to use as a small scale of renewable energy power plant. The energy will be transferred from the renewable energy resource to the compressed air energy by reciprocating air compressor to be stored in a pressurized vessel. The energy storage system uses a small compressed air energy storage system, developed as a small unit and installed above ground to avoid site limitation as same as the conventional CAES does. Therefore, it is suitable to be placed at any location. The system is operated in low pressure not more than 15 bar, so, it easy to available component in country and inexpensive. The power generation uses a variable speed induction generator (IG). The relationship of pressure and air flow of the compressed air, which varies continuously during the discharge of compressed air to drive the generator, is considered as a control command. As a result, the generator generates power in wide speed range. Unlike the conventional CAES that used gas turbine, this system does not have any combustion units. Thus, the system does not burn fuel and exhaust pollution. This paper expresses the modelling, thermodynamic analysis simulation and experiment to obtain the characteristic and performance of a new concept of a small compressed air energy storage power plant, which can be helpful in system designing of renewable energy electrification. The system was tested under a range of expansion pressure ratios in order to determine its characteristics and performance. The efficiency of expansion air of 49.34% is calculated, while the efficiency of generator of 60.85% is examined. The overall efficiency of system of approximately 30% is also investigated.

• 한국유기농업학회지
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• 제27권4호
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• pp.437-452
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• 2019

소규모 식육포장처리장은 일반규모 작업장에 비해 종업원의 숫자가 적기 때문에 전반적인 위생상태 개선 및 HACCP System의 적용을 위해서 일반 규모 작업장에 비해 간단히 적용할 수 있도록 해야 할 필요가 있는 것은 사실이다. 하지만 여전히 축산식품 안전사고가 많이 나타나고 있는 시점에 국민건강 안전을 위해서라도 식육포장처리장에서 많이 발생하는 문제점, 그리고 그 위해의 심각도가 커서 사회·경제적으로 파급 여파가 큰 중요 관리항목에 대해서는 규모가 작은 업소에서도 철저히 준수해야 할 필요가 있다고 판단되며, 이는 HACCP 심사에도 연계하여 그에 대한 평가를 진행할 필요가 있다고 사료된다. 본 연구결과 도출을 위해 지난 3년간의 HACCP 심사 시 지적사항 발생빈도를 분석하고, 기존 논문자료 등을 참고하여 중요도를 결정하여 새로운 소규모 HACCP 평가항목을 도출하였다. 다만, 본 연구에서 개발된 소규모 식육포장처리업 HACCP 평가항목은 통계적, 문헌적 자료를 검토하여 도출한 것으로 실제 소규모 식육포장처리장 작업 현장에서 꼭 평가가 필요한 항목인지에 대해서는 추후 현장적용을 통해 추가적인 검토가 필요할 것으로 사료되고, 현행 『식품 및 축산물 안전관리인증기준』 고시에 따른 일반규모 축산물 HACCP 평가는 업종별 특성에 맞추어 평가항목이 다르게 적용되어 있으나, 소규모의 경우에는 식육판매업 및 식용란수집 판매업을 제외한 다른 업종은 전부 동일한 평가표를 이용하여 평가를 하도록 되어 있어 해당 업종의 특성에 맞도록 평가항목을 분리하는 등의 연구도 필요할 것으로 판단된다. 아울러 인력 부족 등으로 인하여 소규모 식육포장처리장에서 쉽게 적용하기 어려운 위해분석 등 HACCP 관리 평가항목에 대해서는 정부에서 쉽게 적용할 수 있는 모델 개발 등을 통해 소규모 식육포장처리장에서 무리 없이 HACCP을 적용할 수 있도록 유도하는 것이 중요한 것으로 판단된다.

• 한국지하수토양환경학회:학술대회논문집
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• 한국지하수토양환경학회 2001년도 총회 및 춘계학술발표회
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• pp.182-186
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• 2001

An acid forest surface soil as a land farming medium was treated with a water plant alum sludge at 0 to 18%. Indian mustard was grown in the treated soil in a greenhouse for 5 weeks and watered with pH 4 tap water adjusted with a mixed acid (1HNO$_3$: 2H$_2$SO$_4$) during plant growth. Changes in soil property, leachate chemistry, plant growth, and plant uptake of elements by the sludge treatment were determined. The alum sludge treatment increased buffer capacity to acidity, hydraulic conductivity, water holding capacity, and phosphate adsorption of the soil and decreased bulk density and mobility of small particles. The sludge treatment reduced leaching of Al, Mg, K, Na, and root elongation. Plant did uptake less amount of the cations and P but more Ca with the sludge treatment.

• The Plant Pathology Journal
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• 제17권2호
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• pp.88-93
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• 2001

Oxidative stress is one of the major limiting factor in plant productivity. Reactive oxygens species (ROS) generated during metabolic processes damage cellular functions and consequently lead to disease, senescence and cell death. Plants have evolved an efficient defense system by which the ROS is scavenged by antioxidant enzymes such as superoxide dismutase (SOD) and ascorbate peroxidase (APX). Attempts to reduce oxidative damages under the stress conditions have included the manipulation of 갠 scavenging enzymes by gene transfer technology. Increased SOD activities of transgenic plants lead to increased resistance against oxidative stresses derived from methyl viologen (MV), and from photooxidative damage caused by high light and low temperature. Transgenic tobacco plants overexpressing APX showed reduced damage following either MV treatment of photooxidative treatment. Overexpression of glutathion reductase (GR) leads to increase in pool of ascorbate and GSH, known as small antioxidant molecules. These results indicate through overexpression of enzymes involved in ROS-scavenging could maintain or improve the plant productivities under environment stress condition. In this study, the rational approaches to develop stress-tolerant plants by gene manipulation of antioxidant enzymes will be introduced to provide solutions for the global food and environmental problems in the $21^\textrm{st}$ century.

• Journal of Plant Biotechnology
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• 제7권2호
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• pp.87-95
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• 2005

The tomato ripening associated membrane protein (TRAMP) (Fray et al., 1994) is a member of the major intrinsic protein (MIP) family, defined as channels facilitating the passage of water and small solutes through membranes. During normal fruit ripening the TRAMP mRNA levels were increased whereas the expression levels of TRAMP in low ethylene ACO1-sense suppressed lines, Nr and rin fruits, were lower than at the breaker stage of wild type fruit. TRAMP mRNA is inhibited by $LaCl_3$, which is an inhibitor of $Ca^{2+}$-stimulated responses, treatment but drought condition did not affect TRAMP expression. The levels of TRAMP mRNA transcripts were substantially higher in the dark treated seedlings and fruits. These suggest that TRAMP function as a water channel may be doubted because of several reasons; no water content was changed during ripening in wild type, antisense and overexpression lines, TRAMP expression under light condition was lower than dark condition and TRAMP expression was not changed in drought condition. Co-suppression plant, 3588 was one of sense suppression lines, which contain CaMV 35S promoter and sense pNY507 cDNA, produced small antisense RNA, approximately 21-25 nucleotides in length, mediated post-transcriptional gene silencing. Therefore, TRAMP expression was inhibited by small antisense and multiple copies might induce gene silencing without any production of double strand RNA. Total seven selected volatile productions, isobutylthiazole, 6-methyl-5-hepten-2-one, hexanal, hexenal methylbutanal, hexenol, and methylbutanol, were highly reduced in sense line whereas total volatile production was increased in TRAMP antisense line. These results suggested TRAMP might change volatile related compounds.

• Journal of Plant Biotechnology
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• 제44권3호
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• pp.287-295
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• 2017

Water temperature is one of the major factors that impacts the growth and life cycle of Pyropia tenera, one of the most valuable and cultivated marine red algae belonging to Bangiales (Rhodophytes). We analyzed transcriptome from gametophyte of P. tenera under normal and high temperature conditions, and identified four small heat shock proteins (sHSPs). They have no significant amino acid sequence homology with known proteins in public databases except PhsHSP22 from Pyropia haitanensis. PtsHSP19.3 gene responded to high temperature but slightly or not to desiccation, freezing or high salt condition. When the PtsHSP19.3 gene was overexpressed in Chlamydomonas reinhardtii, transformed Chlamydomonas lines revealed much higher growth rate than that of control cells under heat stress condition. Transformed cells also grew well in those of the control cell onto the medium containing high salt or $H_2O_2$. When the PtsHSP19.3 was fused to GFP and introduced into tobacco protoplast, fluorescence was detected at several spots. Results indicate that PtsHSP19.3 may form super-molecular assembles and be involved in tolerance to heat stress.