• Journal of Korean Society on Water Environment
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• v.20 no.5
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• pp.480-487
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• 2004

This study was conducted to find the effect of electron acceptors on the formation of granular sludge by using four different types of electron acceptors. The phosphorous uptake, denitrification, and sulfate reduction in anoxic modes were simultaneously occured because of the presence of the polyphosphate accumultating organism(PAO) that utilize nitrate and sulfate as an electron acceptor in the anoxic zone. Denitrirying phosphorous removal bacteria(DPB) was enriched under anaerobic/anoxic/aerobic condition with a nitrate as an electron acceptor, and desulfating phosphorous removal bacteria(DSPB) was enriched under anaerobic/anoxic/aerobic condition with a sulfate as an electron acceptor. Polyphosphate accumulating organism(PAO) were enriched in the anaerobic/aerobic SBR. PAO took up acetate faster than DPB and DSPB during the aerobic phase. The sludge with nitrate and sulfate as an electron acceptors grew as a granules which possessed high activity and good settleability. In the anaerobic/aerobic modes, typical floccular growth was observed. In the result of bench-scale experiment, simultaneous reactions of phosphorus uptake, denitrification and sulfate reduction were observed under anoxic condition with nitrate and sulfate as an electron acceptors. These results demonstrated that the anaerobic/anoxic modes with nitrate and sulfate as an electron acceptors played an important role in the formation of the sludge granulation.

• Journal of Korean Society of Water and Wastewater
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• v.22 no.2
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• pp.219-225
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• 2008

Evaluating and designing secondary clarifier require to define characteristics of influent MLSS (Mixed Liquor Suspended Solids). In this study, bioflocculation and settling characteristics for MLSS from a Biological Nutrient Removal (BNR) plant located in near Seoul were measured. MLSS concentrations in bioreactor were about 2,500mg/L in summer and about 4,000mg/L in winter, respectively. Tests showed that there was not much bioflocculation occurred in secondary clarifier. Average ESS/DSS (Effluent Suspended Solids/Dispersed Suspended Solids) was 100%. From the settling tests, ZSV (Zone Settling Velocity) and settling constant (n) in Vesilind equation were estimated at different MLSS temperatures. SVI (Sludge Volume Index) and SSVI (Stirred Sludge Volume Index) were also measured at different temperatures. It was found that ZSV was positively correlated with temperature and n was inversely proportional to temperature. SVI and SSVI had very similar values at about $25^{\circ}C$ of MLSS, However, SVI had more than 2 times higher values than SSVI at below $20^{\circ}C$ of MLSS. Temperature effect must be considered to design and evaluate secondary clarifier.

• Journal of Korean Society of Water and Wastewater
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• v.8 no.1
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• pp.18-26
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• 1994

This research investigated efficient operation mode for the successful performance of SBR(sequencing batch reactor) treating fish processing wastewater, and the effect of sodium chloride (NaCl) on treatment efficiency. 2-hour-annerobic, 6-hour-aerobic and 3-hour-anoxic operation during reaction period was found an effective operating method for organic and nitrogen removal from fish processing wastewater in SBR system. The average removal efficiencies of COD, BOD, and total nitrogen in SBR operated continuousely were 91%, 95%, and 67.1%, respectively. The estimated values of biomass yield coefficient(Y), microbial decay coefficient($K_d$), and bioreaction rate constant(K) were $0.35gMLSS/gCOD_{removed}$, $0.015day^{-1}$, and $0.209hr^{-1}$, respectively. As NaCl concentration increased from 5 to 30g/L, sludge settleability was cnhanced but organic removal in the reactor was decreased. NaCl of influent had considerable relationship with COD removal, whereas it did not significant affect nitrogen removal.

• Journal of Korean Society of Environmental Engineers
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• v.30 no.2
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• pp.168-174
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• 2008

Diagnosis on setteability based on human operator's experimental knowledge, which could be established by long term operation, is a limit factor to construction of automation control system in wastewater treatment plant. On-line SVI(Sludge Volume Index) analyzer was developed which can measure SV30 automatically by image capture and image analysis method. In this paper, information got by settling process was studied using On-line SVI analyzer for better operation & management of WWTPs. First, SV30 detection algorithm was developed using image capture and image analysis for settling test and it showed that automatic detection is feasible even if deflocculation and bulking was occurred. Second, turbidity assessment model was developed using image analysis.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.8 no.3
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• pp.627-631
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• 2007

The factors affecting on sludge sedimentation are reported as F/M ratio, ingredient, composition of influent substrate, dissolved oxygen concentration, temperature, pH, filamentous bacteria and SRT. Aeration tank applying Bacillus sp. has an important role for maintaining the dominant microorganism species to make steady progress for spore growth affecting sedimentation. This research aims to investigate the affecting factor for the sedimentation in B3 system and RABC system with aeration tank applying tapered aeration. Extracellular polymeric substances(EPS), protein and carbohydrate can be produced for the extreme condition, that is down to 0.2 mg/L of dissolved oxygen in the aeration tank. This research found out the relation between the sedimentation and the EPS production, especially the ratio of protein/carbohydrate. The spore of Bacillus sp. was formed at the low DO then microorganisms produced EPS. The results showed that the production of EPS was 109.95 mgEPS/gSS at 1.6 mg/L of DO, however it was 131.77 mgEPS/gSS at 0.5 mg/L of DO. The sedimentation was affected by protein content in EPS and the ratio of protein and carbohydrate. The settleability of sludge was not affected by the ratio of protein/carbohydrate in B3 process, meanwhile settleability was affected by the ratio of it in RABC process, respectively.

• Journal of Korean Society of Environmental Engineers
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• v.22 no.6
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• pp.1027-1035
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• 2000

This study was carried out to investigate the treatability of landfill leachate having high concentration of ammonium nitrogen with/without the circulation of media in pilot-scale($48m^3basis$) process. Total nitrogen removal efficiency was relatively increased in the media added process (influent ; $1.230{\sim}2,000mg{\cdot}l^{-1}$, effluent ; $120{\sim}250mg{\cdot}l^{-1}$) compared with the control process. The difference of nitrogen removal efficiency between these processes may be due to that stable growth of nitrifiers attached to the media could be achieved 99.3% of ammonium-nitrogen removal efficiency(without ; 98.2%) and 88.5% of total nitrogen removal efficiency(without ; 85.8%) were shown in media added process, respectively. Also, optimum BOD/ $NH_4{^+}$-N ratio was relatively decreased in the media process compared with the control process. Sludge settleability, on the other hand. was shown better in media added process than in control process. This outstanding sludge settleability in the media added process indicates the compatibility of media(zeolite) to the microorganism and the possibility of using media of biofilm process.

• Journal of Korean Society of Water and Wastewater
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• v.8 no.2
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• pp.25-34
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• 1994

In this research, investigations were made on the effect of type and load of organic substrate on phosphorus release. Reactors of three different sizes were operated, being fed on five kinds of organic substrates. The quantitative analyses were made on phosphorus release and substrate utilization under anaerobic condition. The molar ratios of the uptaken organic substrate to the released phosphorus were 0.5 with acetate, 0.6 with glucose, 0.8 with glucose/acetate, and 1.2 with glucose/acids, respectively. The phosphorus release was inhibited at the higher organic load than the normal at stead state. Both acetate and acids/glucose enhanced phosphorus release- as well as uptake-rate, however, the complete phosphorus removal was achieved after the microbial adaptation to the new environment. In case with acetate, operation was hampered by the poor sludge settleability and phosphorus uptake was not enough although the phosphorus release was active. But with milk/starch, the phosphorus release and uptake was well developed even though phosphorus release was not comparatively high. From this study, it was concluded that organic substrates, such as glucose seemed to be converted fatty acids after fast bio-sorption, followed by concurrent uptake of these acids by excess phosphorus removing bacteria.

• Journal of Korean Society on Water Environment
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• v.20 no.2
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• pp.190-195
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• 2004

A new technology for advanced wastewater treatment was developed using a modified Rotating Biological Contactor (RBC) process, named as Rotating Activated Bacillus Contactor (RABC) process that utilizes Bacillus sp., the facultatively anaerobic or activated microaerophilic bacteria on multiple-stage reticular rotating carriers, as a predominant species. The RABC process for a municipal wastewater with relatively low concentrations of organics, nitrogen, and phosphorus showed stable and high removal efficiencies, less than $BOD_5$ 10 mg/L, T-N 15 mg/L, and T-P 1.5 mg/L in final effluent. The performance load of RABC process was shown to be $1.23kg{\cdot}BOD/m^2{\cdot}day$ for the first stage (average $0.31kg{\cdot}BOD/m^2{\cdot}day$ for the total stages) based on both removed BOD and converted disc area corresponding to the reticular one. The sludge produced in the RABC process is characterized by low generation rate (about $0.18kg{\cdot}MLSS/kg{\cdot}BOD$) and excellent settleability. The number ratio of Bacillus ($2.4{\times}10^6CFU/ml$) to heterotrophic bacteria ($3.6{\times}10^7CFU/ml$) inhabiting in the biofilms of the RABC process was 6.7 %, indicating that Bacillus sp. was a predominant species in the biofilms. The RABC process with reticular rotating carriers showed its excellent performance for the advanced wastewater treatment without any offensive odor problem due to organic overloading.

• Journal of Korean Society of Environmental Engineers
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• v.32 no.3
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• pp.234-240
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• 2010

The effectiveness of adding powdered zeolite and granular activated carbon (GAC) before starvation into biological reactor for recovering its performances was investigated. Two types of carrier addition in Sequencing Batch Reactor (SBR) system for non-saline and saline wastewater were evaluated after starvation periods. During the experiment, settleablity (SVI), floc size, fractal dimension, $COD_{Mn}$, T-N, T-P removal efficiencies and recovery time were monitored. When the wastewater feeding was resumed after starvation period for 5days, the SVI increased at the beginning of resumption and then decreased with time in both types. And the larger the floc size and fractal dimension of floc, the more increased removal efficiency for $COD_{Mn}$, T-N and T-P was also. Its performance recovery was strongly correlated with floc size and fractal dimension of activated sludge. After resuming the wastewater feeding, the SVI, floc size, fractal dimension, $COD_{Mn}$, T-N, T-P removal efficiency of SBR with carrier improved and reached its initial value faster compared to those of SBR without carrier.

• Journal of Environmental Science International
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• v.3 no.3
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• pp.273-284
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• 1994

The primary objective of this study was to examine the toxic effects of PCP on activated sludge and to analyze its metabolic responses while treating wastewater containing pentachlorophenol (PCP) in a sequencing batch reactor (SBR) system operating under different control strategies. This study was conducted in two phases 1 and 2 (8-hr and 12-hr cycles). Each phase was operated with two control strategies I and II. Strategy I (reactor 1) involved rapid addition (5 minutes to complete) of substrate to the reactor with continuous mixing but no aeration for 2 hours. Strategy ll (reactor 2) involved adding the feed continuously during the first 2 hours of the cycle when the system was mixed but not aerated. During both phases each reactor was operated at a sludge age of 15 days. The synthetic wastewater was used as a feed. The COD of the feed solution was about 380 mg/l. After the reference response for both reactors was established, the steady state response of each system was established for PCP feed concentrations of 0.1 mg/l, 1.0 mg/l, and 5.0 mg/l in SBR systems operating on both 8-hr and 12-hr cycles. Soluble COD removal was not inhibited at any feed PCP concentrations used. At 5.0 mg/l fined PCP concentration and in SBR systems operating on phase 2, the concentrations of MLVSS were decreased; selective pressure on the mixed biomass might be increased, narrowing the range of possible ecological responses; the settleability of activated sludge was poor; the SOURS were increased, showing that the systems were shocked. Nitrification was made to some extent at all concentrations of feed PCP in SBR systems operating on phase 2 whereas in SBR systems operating on phase 1 little nitrification was observed. Then, nitrification will be delayed as much as soluble COD removal is retarded due to PCP inhibition effects. Enhanced biological phosphorus removal occurring in the system operating with control strategy I during phase 1 of this work and in the presence of low concentrations of PCP was unreliable and might cease at anytime, whereas enhanced biological phosphorus removal occurring in the system operating with either control strategy I or II during phase 2 of this work and in the Presence of feed PCP concentrations up to 1.0 mg/l was reliable. When, however, such processes were exposed to 5.0 mg/l PCP dose, enhanced phosphorus removal ceased and never returned.