• 대한약리학회지
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• 제17권1호
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• pp.9-15
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• 1981

부자성분중 강심효과를 나타내는 부자부타놀분획의 작용기전을 심근수축기전이 있어서 중심적인 역활을 하는 칼슘이온과의 상호관계에서 검토하였으며 아울러 동물생체내 혈동력학에 대한 영향을 관찰하였다. 부자부타놀분획 적출 고양이 유두근표본의 수축력증가작용에 있어서 심근외칼슘과 상승적이었으며 부자부타놀분획 $10^{-4}g/ml$는 칼슘 0.06mM과 동등한 심근수측증강 효과를 나타내었다. 심근세포막의 칼슘통로 억제약물인 verapamil 존재하에서 저하된 유두근 수축력은 부자부타놀분획에 의하여 용량의존적으로 회복되었으며, 이로부터 부자부타놀분획의 강심작용기전의 일부는 심근세포막의 완만내향성 칼슘통로를 통한 칼슘유입을 측진하는 것과 관계가 있을 것으로 추측하였다. 정상가토의 혈동력학에 있어서 부자부타놀분획은 디지탈리스배당체와는 달리 용량의존적으로 전신동맥압을 저하시키고, 경동맥혈류량을 증가시켰으며, 심박동수에는 크게 의미있는 변화를 보이지 않았다.

• 대한수의학회지
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• 제29권3호
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• pp.275-281
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• 1989

This investigation was conducted on 249 Jindo dogs aging from 2 to 12 months to determine body type and external appearance of the dogs. The results of this investigation were as follows. 1. The withers height of male dogs growed rapidly during the period between 2 months and 7 months of age, and was 22.94cm and 49.77cm, respectively. Afterward the growth rate slowed down up to 12 months, 53.33cm. The growth rate of female was similar to male, and the withers height was 22.91cm, 46.81cm, 48.70cm at 2, 6 and 12 months of age. 2. For male and female the body length was 27.21, 27.23cm at 2 months, 51.40cm, 51.62cm at 6 months, 58.33, 52.15cm at 12 months, respectively. Growth rate of the body length was very rapid from 2 to 6 months of age, but afterwards the growth rate was slow and somewhat retarded. 3. For male and female, the withers height to body length ratio was 100 : 109.4 and 100 : 107.1 in 12 months old group. 4. Cross over between the sexes in growth rate occurred between 5.3 to 6.5 months of age for wither height, between 6.0 to 7.2 months for hody length, respectively. 5. The average chest girths of male and female were 31.46, 30.46cm at 2 months, 54.92, 52.20cm at 7months, 60.25, 57.90cm at 12months, respectively. The growth rate of chest girth was rapid between 2 to 7 months of age and gradual between 7 to 12 months. 6. The average skull lengths of male and female were 11.76, 11.32cm at 2 months, 21.83, 19.60cm at 12 months, respectively. The average head widths of male and female were 8.37, 7.94cm at 2 months, 15.47, 12.46cm at 12 months. 7. Erected type of ear was completed from oblique type at 6 months in male and 7 months in female. On our detailed examinations, we concluded that Jindo dogs completed their growth at 6 to 7 months of age.

• 한국균학회지
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• 제9권4호
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• pp.199-207
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• 1981

This investigation was undertaken to establish more accurately the clinical features of the desease as seen in Korea, and to investigate susceptibility factors and to study the ecology of P. orbiculare in this condition. All of 50 new patients with Tinea versicolor attending the Ewha University Hospital, during a period of 6 months were studied personally. 1. Clinical feature: The sex ratio of the whole series was 32 males to 18 females. Twenty of 50 patients (40%) were between the age of 20 to 29 years. The duration of disease was more than 4 months in 37 of 50 patients (74%). The cases of first onset in June, July and August were 33 of 50 patients (66%). Whereas, the developed in December, January and February were only 3 of 50 patients (6%). Seasonal fluctuation was remarkable in 16 of 25 patients (66.7%) who had the duration of more than 1 year. The course of disease was rapid in 33 of 50 patients (66%) and slow in 9 of 50 patients (18%). Thirty-three of 50 patients (66%) had not any subjective symptoms. Thirteen patients (26%) had mild itching sensation and 4 patients (8%) had severe itching. The frequency of the first affected were neck (27.4%), axilla (19.2%) anterior chest (15.0%) and groin (11.0%). 7) Hyperpigmented lesions were observed in 35 of 50 patients (70%) and hypopigmented lesions were observed in 11 of 50 patients (22%). Skin lesions were noticed mainly in the anterior chest, neck, back and axilla, and more severe in the anterior chest, back and abdomen than other areas. Tinea versicolor was particularly prevalent in those persons who had occupations of mental work and who perspired every day or every 2 or 3 days. A positive family history of Tinea versicolor was obtained in 7 of 50 patients (14%). Fourteen of 50 patients (28%) had association with superficial fungal infections. 2. Mycological features: Typical appearance of M. furfur was observed in all scrapings from 25 lesions and only 3 scrapings from 20 clinically skin of the patients. P. orbiculare was seen on direct examination of scrapings from 24 of 25 skin lesions, from 19 of 20. clinically normal skin of patients and from 18 of 29 control normal skin. The quantity of P. orbiculare was greater in skin lesions and clinically normal skin area than in control normal skin surfaces.

• 대한약리학회지
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• 제26권1호
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• pp.35-49
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• 1990

A highly purified $(Na^+,\;K^+)-ATPase$ from the rectal gland of Squalus acanthias and from the electric organ of Electrophorus electricus has been used to raise antibodies in rabbits. The 97,000 dalton catalytic subunit and glycoprotein derived from the rectal gland of spiny shark were also used as antigens. The two $(Na^+,\;K^+)-ATPase$ holoenzymes and the two shark subunits were antigenic. In Ouchterlony double diffusion experiments, these antibodies formed precipitation bands with their antigens. Antibodies prepared against the two subunits of shark holoenzyme also formed precipitation bands with their antigens and shark holoenzyme, but not with eel holoenzyme. These observations are in good agreement with inhibitory effect of these antibodies on the catalytic activity of $(Na^+,\;K^+)-ATPase$ both from the shark and the eel, since there is very little cross-reaction between the shark anticatalytic subunit antibodies and the eel holoenzyme. The maximum antibodies titer of the anticatalytic subunit antibodies is found to be 6 weeks after the initial single exposure to this antigen. Multiple injections of the antigen increased the antibody titer. However, the time required to produce the maximum antibody titer was approximately the same. These antibodies also inhibit catalytic activity of $(Na^+,\;K^+)-ATPase$ vesicles reconstituted by a slow dialysis of cholate after solubilization of the enzyme in a presonicated mixture of cholate and phospholipid. In these reconstituted $(Na^+,\;K^+)-ATPase$ vesicles, effects of these antibodies on the fluxes of $Na^+$, $Rb^+$, and $K^+$ were investigated. Control or preimmune serum had no effect on the influx of $^{22}Na^+$ or the efflux of $^{86}Rb^+$. Immunized sera against the shark $(Na^+,\;K^+)-ATPase$ holoenzyme, its glycoprotein or catalytic subunit did inhibit the influx of $^{22}Na^+$ and the efflux of $^{86}Rb^+$. It was also demonstrated that these antibodies inhibit the coupled counter-transport of $Na^+$ and $K^+$ as studied by means of dual labeling experiments. However, this inhibitory effect of the antibodies on transport of ions in the $(Na^+,\;K^+)-ATPase$ vesicles is manifested only on the portion of energy and temperature dependent alkali metal fluxes, not on the portion of ATP and ouabain insensitive ion movement. Simultaneous determination of effects of the antibodies on ion fluxes and vesicular catalytic activity indicates that an inhibition of active ion transport in reconstituted $(Na^+,\;K^+)-ATPase$ vesicles appears to be due to the inhibitory action of the antibodies on the enzymatic activity of $(Na^+,\;K^+)-ATPase$ molecules incorporated in the vesicles. These findings that the inhibitory effects of the antibodies specific to $(Na^+,\;K^+)-ATPase$ or to its subunits on ATP and temperature sensitive monovalent cation transport in parallel with the inhibitory effect of vesicular catalytic activity by these antibodies provide direct evidence that $(Na^+,\;K^+)-ATPase$ is the molecular machinery of active cation transport in this reconstituted $(Na^+,\;K^+)-ATPase$ vesicular system.

• Clinical and Experimental Reproductive Medicine
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• 제30권3호
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• pp.241-248
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• 2003

Objective: The purpose of this study was to evaluate the survival rate of vitrified blastocyst according to the freezing vessels, equilibration time in cryoprotectant and artificial dehydration method. Methods: Human blastocysts were vitrified after loading onto the plastic straw, open-pulled straw (OPS), electron microscopy grid (EM grid) for 1.5 min or 3 min. They also were directly plunged into LN2 within 30sec. For artificial shrinkage of blastocysts, 36 gauge fine needle was pushed at the cellular junction of the trophectoderm into the blstocoele cavity until it shrank without damage of inner cell mass. Results: The survival rate of vitrified blastocysts on plastic straw, OPS, EM grid as freezing vessels were 26.7, 13.0 and 60.5%, respectively. The survival rate of EM grid was significantly higher than that of plastic straw and OPS (p<0.05). For 1.5 min equilibrium, the survival rates of early blastocyst (EB), middle blastocyst (MB) and late blastocyst (LB) were 64.4, 81.0, and 20.0% respectively. For 3 min equilibrium, the survival rates of EB, MB, and LB were 69.9, 50.0 and 57.5% respectively. The survival rates of EB and MB were significantly higher than that of LB in 1.5 min equilibrium group (p<0.05), however, the significance was not observed in 3 min equilibrium groups. In cytoplasmic shrinkage before vitrification, the survival rates of EB, MB and LB were 92.9, 100 and 75.9% respectively. The survival rate of MB was significantly higher than that of LB (p<0.05). The survival rates of vitrified blastocysts by artificial dehydration and slow-frozen blastocysts were not significantly different as 88.9 and 66.7%, respectively. Conclusion: This study showed that the vitrification of human blastocysts using EM grid and artificial dehydration is an effective method. Therefore, these methods would be an useful techniques for blastocyst cryopreservation.

• 대한수의학회지
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• 제26권1호
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• pp.175-185
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• 1986

The measurement of angled erythrocyte sedimentation rate (ESR), as a replacement for perpendicular ESR, for cattle blood was scrutinized since it has been well known that perpendicular ESR in cattle is too slow to be adopted as an effective clinical test. Samples of blood were taken from 186 Korean native cattle over 2 years old. The results obtained in the experiment were summarized as follows. 1. Average values of perpendicular ESR/24hrs in 15 apparently healthy cattle, as measured by Wintrobe, Westergren and capillary tubes, were $5.8{\pm}2.2$, $11.1{\pm}3.7$ and $10.4{\pm}4.5%$ respectively, which were found to be similar to the values of perpendicular ESR/hr of normal blood of human. 2. The ESR was determined in the tubes held at 90, 75, 60, 45, 30 and 15-degree angles, using 3 types of tubes. For the diagnostic purposes, the best results were obtained from the tubes held at 45-degree angle. 3. The angled ESR values increased as the diameters of the tube-bores decreased. 4. The tube length did not affect the angled ESR(%). 5. The angled ESR values increased with the increased environmental temperature during the ESR measurement. 6. The storage temperature at $5^{\circ}C$, $20^{\circ}C$ and $35^{\circ}C$, of the blood for 24 hours did not affect the angled ESR. 7. Samples of blood were treated with 4 kinds of anticoagulants (heparin, $K_2$-EDTA, double oxalate and sodium citrate) and the ESR was determined at 45-degree angle, using capillary hematocrit tubes. The ESR values were higher in the blood samples treated with sodium citrate than in those treated with other anticoagulants. 8. By using the autologous plasma, the PCV was adjusted to be 5, 10, 20, 30, 40 and 50ml/100ml and the ESR was determined in the capillary hematocrit and Wintrobe tubes held at 45 degrees. In both of the methods the ESRs increased as the values of PCV decreased. The regressions of ESR to PCV in both 45-degree-angled capillary and Wintrobe tubes were curvilinear. For the capillary hematocrit tubes the second degree polynomial $Y=61.9779-2.3533x+0.0228x^2$ (r=0.9999) fits the data. And in the case of Wintrobe tubes the second degree polynomial $Y=27.9767-1.1314x-0.0117x^2$ (r=0.9998) fits the data. 9. The 45-degree angled ESR was determined in the blood of 71 healthy Korean native cows using capillary hematocrit tubes. The average PCV was $35.4{\pm}3.6ml/100ml$. The observed ESR/hr averaged $7.2{\pm}2.7%$, while the corrected ESR/hr to a PCV of 36ml/100ml averaged $6.6{\pm}1.3%$. From these results it was concluded that to obtain the best results the ESR/hr of Korean native cattle should be determined at 45-degree angle at room temperature($20^{\circ}C$) using capillary hematocrit tubes.

• Journal of the Korean Academy of Child and Adolescent Psychiatry
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• 제11권1호
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• pp.91-99
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• 2000

목 적：주의력결핍 과잉운동 장애의 진단과 치료 효과를 평가하기 위해 전산화된 한국판 주의력 장애 진단 시스템(ADHD Diagnostic System：ADS)을 개발한 후, 연령별 규준을 산출하였다. 방 법：서울, 경기도, 강원도 지역의 유치원, 초등학교, 중학교에 재학중인 5세에서 15세 연령범위의 총 847명(남자 429명, 여자 418명)의 정상 아동 및 청소년이 규준집단으로 연구에 참여하였다. 또한 ADS의 신뢰도와 변별 타당도를 검증하기 위해 서울대학병원 소아정신과에서 DSM-IV 기준에 의해 ADHD로 진단받은 아동 30명이 본 연구에 포함되었다. 정상 집단의 아동들에게는 그들이 다니는 학교의 컴퓨터실에서 ADS를 실시하였고, ADHD 아동들에게는 소아정신과 외래 임상심리실에서 ADS를 실시한 후, 정상 아동의 자료에 입각해서 연령별 규준을 산출하였고, 신뢰도와 타당도 분석을 하였다. 결 과：ADS의 내적 일치도 계수(Cronbach's ${\alpha}$)는 .85이었다. 정상 집단과 ADHD 집단간에 오경보 오류를 제외한 모든 측정치에서 유의미한 차이가 있었다. 요인분석 결과, ADS 시각, 청각 검사에서 모두 3개의 요인이 산출되었다. 요인 1은 '주의력 요인'으로 누락오류, 오경보 오류, 민감도(d')가 포함되었고, 요인 2는 '정보처리 속도 요인'으로 정반응시간, 정반응시간의 표준편차가 여기에 해당되며, 요인 3은'충동성 요인'으로 반응기준(${\beta}$)이 포함되었다. 요인 1, 2, 3은 ADHD의 핵심적 증상과 정확하게 일치하므로, 이상과 같은 요인분석 결과는 ADS가 주의집중력 장애를 도구로써 구성 타당도가 있음을 입증해주었다. 판별분석 결과, 시각, 청각 ADS가 정상 아동과 ADHD 아동의 96.7%를 정확하게 변별해 주는 것으로 나타났다. 논 의：ADS가 주의력 결핍-과잉 운동 장애를 일관성이 있게 평가하는 신뢰롭고, 타당한 검사로 입증되었고, ADS를 통해 정상 집단과 ADHD 집단을 정확하게 분류할 수 있음이 시사되었다.

• 한국응용곤충학회지
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• 제47권1호
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• pp.9-15
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• 2008

2003년부터 2006년까지 충남 공주, 부여, 청양 지역에서 구입한 밤에서 탈출하는 밤바구미(Curculio sikkimensis) 유충의 탈출시기, 토양으로 잠입시간, 토중 분포상황 및 월동유충의 생존율과 성충의 우화시기 및 우화율, 성비 및 생존일수를 조사하였다. 밤바구미 유충의 탈출 시기는 조생종이 9월 중순부터 10월 초순, 중생종이 9월 하순부터 10월 중순, 만생종이 10월 초순부터 11월 중순에 탈출하였고, 탈출 후 35분 내에 100% 토양 속으로 잠입하였다. 밤바구미의 월동 유충은 지표면으로부터 48 cm 이내의 깊이에서 서식하였고, 18-36 cm의 깊이에서 74.1%의 분포를 보였다. 월동 유충의 생존율은 1년차에 38.0%, 2년차에 16.0%, 3년차에 2%로 조사되었다. 밤바구미 성충의 우화시기는 8월 초순부터 10월 초순까지로 우화최성기는 9월 초 중순이었다. 우화율은 1년차에 8.4%, 2년차에 3.6%, 3년차에 0.8%로 감소하였다. 성충의 성비는 $0.51{\sim}0.55$ 범위로 나타났고 암컷의 비율이 높았다. 생존일 수는 암컷이 평균 9.9일로 수컷의 8.9일보다 길어 암컷의 수명이 더 긴 것으로 나타났다.

• 한국응용약물학회:학술대회논문집
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• 한국응용약물학회 2001년도 추계학술대회 및 정기총회
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• pp.95-95
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• 2001

Ciinidipine (FRC-8635) is a newly synthesized novel DHP type of organic Ca$\_$2＋/channel blockers that have been developed so far in Japan (Yoshimoto et al., 1991 : Hosono et at., 1992). It also has a blocking action on L-type voltage-dependent Ca$\^$2＋/channel (VDCCs) in the rabbit basilar artery (Oike et al., 1990) and a slow-onset and long-lasting hypotensive action in clinical and experimental studies (Ikeda et al., 1992 ; Tominaga et al., 1997). Recent electrophysiological data indicate that cilnidipine might be a dual-channel antagonist for peripheral neuronal N-type and vascular L-type Ca$\^$2＋/channels (Oike et al., 1990 ; Fujii et al., 1997; Uneyama et at., 1997). However, little is known about the involvement of N-type VDCCs in contributing to the muscarinic receptor-mediated CA secretion. Therefore, the present study was attempted to investigate the effect of cilinidipine on secretion of catecholamines (CA) evoked by ACh, high K$\^$＋/, DMPP and McN-A-343 from the isolated perfused rat adrenal gland. Cilnidipine (1-10 ${\mu}$M) perfused into an adrenal vein for 60 min produced dose- and time-dependent inhibition in CA secretory responses evoked by ACh (5.32${\times}$10$\^$-3/M), DMPP (10$\^$-4/ M for 2 min) and McN-A-343 (10$\^$-4/ M for 2 min). However, lower dose of lobeline did not affect CA secretion by high K$\^$＋/(5.6${\times}$10$\^$-2/ M), higher dose of it reduced greatly CA secretion of high K$\^$＋/. Cilnidipine itself did also fail to affect basal catecholamine output. Furthermore, in adrenal glands loaded with cilnidipine (10 ${\mu}$M), CA secretory response evoked by Bay-K-8644 (10 ${\mu}$M), an activator of L-type Ca$\^$2＋/channels was markedly inhibited while CA secretion by cyclopiazonic acid (10 ${\mu}$M), an inhibitor of cytoplasmic Ca$\^$2＋/-ATPase was no affected. Moreover, $\omega$-conotoxin GVIA (1 ${\mu}$M), given into the adrenal gland for 60 min, also inhibited time-dependently CA secretory responses evoked by ACh and high K$\^$＋/.

• Clinical and Experimental Reproductive Medicine
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• 제24권1호
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• pp.111-118
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• 1997

Intracytoplasmic sperm injection (ICSI) recently has been utilized widely as the most successful technique to overcome the unfertilization problem in cases of severe male infertility in couples who could not be treated by conventional IVF. Recently, indications of ICSI have been extended further and more fertilized oocytes become available. Thus, it is necessary to examine the efficiency of freezing the surplus embryos obtained from ICSI. We compared the survival rate and the future outcome of cryopreserved embryos obtained either after conventional IVF or ICSI during the same period. After ICSI or IVF, five best-quality embryos from each patient were transferred in the stimulation cycle and the surplus pronuclear (PN) stage oocytes or multicellular embryos were cryopreserved by slow freezing protocol with 1,2-propanediol (PROH) as a cryoprotectant. A total of 792 embryos from ICSI trial were thawed and 65.2% (516/792) survived. The survival rates of PN stage oocyte, multicellular embryo and PN + multicellular embryo were 63.5%, 68.2%, 64.0%, respectively. After 111 transfers, 34 pregnancies were achieved, corresponding to a clinical pregnancy rate of 30.6% per transfers. We thawed 1033 embryos from IVF trials and 57.5% (594/1033) survived. In IVF cycle, the survival rates of PN stage oocyte, multicellular embryo and PN + multicellular embryo were 58.2%, 65.2%, 40.2%, respectively. Thirty eight clinical pregnancies were established after 134 transfers, corresponding to a pregnancy rate of 28.4% per transfer. The cleavage rate of thawed PN stage oocytes from ICSI trial (61.3%) was significantly higher than those from conventional IVF (53.4%). The developmental rates of good embryo (${\geqq}$ grade II) in thawed PN stage oocytes obtained from conventional IVF and ICSI were 63% and 65%, respectively. We concluded that PN stage oocytes, multicellular embryos resulting from ICSI procedure can be successfully frozen/thawed with reasonable clinical pregnancy rates comparable to those of IVF.